ABSTRACT
The lectins of associative nitrogen-fixing bacteria Azospirillum brasilense Sp7 and its mutant A. brasilense Sp7.2.3 were shown to have different effects on the components of the wheat seedling root signal system, namely to regulate the levels of cAMP, nitric oxide, diacylglycerol, and salicylic acid, as well as to induce the activities of superoxide dismutase and lipoxygenase. Our results make it possible to consider azospirilla lectins as inducers of the signal systems in wheat seedling roots, since they cause development of several flows of primary signals. These data are of general biological importance, since lectins are present in all living organisms and most ot the functions of lectins remain insufficiently understood.
Subject(s)
Azospirillum brasilense/physiology , Lectins/metabolism , Plant Roots/metabolism , Seedlings/metabolism , Triticum/microbiology , Azospirillum brasilense/genetics , Diglycerides/metabolism , Lectins/pharmacology , Lipoxygenase/metabolism , Nitric Oxide/metabolism , Plant Roots/drug effects , Plant Roots/microbiology , Seedlings/drug effects , Seedlings/microbiology , Signal Transduction , Superoxide Dismutase/metabolism , Triticum/drug effects , Triticum/metabolismABSTRACT
Some optimized methods of purifying actin from animal, plant, and bacterial cells were developed. Variants of the preparation of antiactin antibodies are described which use both traditional methods of immunization and phase display technology and antigen adsorption on colloidal gold particles. The conjugates of colloidal gold with phalloidin and heavy meromyosin as well as with antibodies were proposed. It was shown that these markers make it possible to reliably identify actins of different origin by the methods of light, and electron microscopy and dot-analysis.
