[Assay of adenine nucleotide level in platelet storage pool for evaluation of the course of hemoblastosis]. / Issledovanie urovnia adeninnukleotidov pula khraneniia trombotsitov dlia otsenki techeniia gemoblastozov.

The content of adenine nucleotides (ATP, ADP) was studied in dense granules of platelets in hemoblastosis to estimate the character of the pathological process course. Varying biochemical defects were observed at the levels of ATP and ADP depending on the severity of the pathological process in chronic myeloid leukemia, acute lymphoblastic leukemia, acute myeloblastic and acute myelo-monoblastic leukemias. Adenine nucleotide values can be used for the diagnosis of varying stages of the above diseases, for the evaluation of anomalous platelets and characterization of the adequacy of the bone marrow hemopoiesis.

[Importance of studying the level of platelet adenine nucleotide levels in various phases of chronic myeloleukoses]. / Znachenie issledovaniia urovniia adeninnukleotidov trombotsitov v raznykh fazakh zabolevaniia khronicheskim mieloleikozom.

Content of adenine nucleotides was studied in various compartments of blood platelets obtained from donors and patients with chronic myeloleukosis at various steps of the disease. Dissimilar biochemical defect was found in content of ATP and ADP, which varied in thrombocyte storage granules depending on severity of the disease. The data on adenine nucleotides content may be used for diagnosis of various steps of chronic myeloleukosis, for detection of thrombocytes anomaly and for evaluation of the state of medullary hemopoiesis.

[Comparative characteristics of the catalytic properties of platelet adenosine deaminase in normal states and in chronic myeloid leukemia]. / Sravnitel'naia kharakteristika kataliticheskikh svoistv adenozindezaminazy trombotsitov v norme i pri khronicheskom mieloleikoze.

Properties of adenosine deaminase from thrombocytes of healthy persons and of patients with chronic myeloleukosis were studied in presence of various structural analogues of purines and metal ions as well as at various temperature. Catalytic properties of the enzyme in thrombocytes of patients with chronic myeloleukosis were distinct from those of thrombocytes of healthy donors. These differences in the enzymatic properties (contrary to standard estimation of the enzyme activity) might be used both as a criterion of adenosine deaminase anomalies and for detection of the initial steps of the disease.

[The role of adenine nucleotides in the functional activity of platelets in hemophilia A]. / Znachenie issledovaniia adeninnukleotidov v funktsional'noi aktivnosti trombotsitov pri gemofilii A.

Content of adenine nucleotides was studied in various pools of thrombocytes from donors and from patients with hemophilia A. Biochemical deficiency of adenine nucleotides appears to occur in the pool of thrombocytes obtained from patients with hemophilia A; estimation of ADP and ATP in the storage granules as well as of the nucleotides specific radioactivity may serve as a criterion of thrombocyte disfunction.

[Adenine phosphoribosyltransferase and hypoxanthine phosphoribosyltransferase of blood platelets in hereditary coagulopathies]. / Adeninfosforiboziltransferaza i gipoksantin-guaninfosforiboziltransferaza trombotsitov krovi pri nasledstvennykh koagulopatiiakh.

Activities of adenine phosphoribosyltransferase (EC 2.4.2.7 APRT) and hypoxanthine-guanine phosphoribosyltransferase (EC 2.4.2.8 HGPRT) were studied in thrombocytes of healthy donors, patients with hemophilia A and B and of women--heterozygote carriers of the pathologic gene. The data obtained suggest that HGPRT test may be used as a genetic marker of hemophilia as well as to detect the heterozygote carriers; estimation of APRT activity is suitable test for differentiation of hemophilia forms.

[Platelet adenosine desaminase in various hematological diseases]. / Adenozindezaminaza trombotsitov krovi pri razlichnykh gematologicheskikh zabolevaniiakh.

Activity of adenosine deaminase (EC 3.5.4.4) was studied in thrombocytes of donors and patients with various hematological diseases. The enzymatic activity was decreased in acute leukemia, chronic myeloleukemia, chronic leukemia and blast transformation myeloma, microspherocytic and hypoplastic anemias. Variable level of the activity was observed in chronic lympholeukemia and non-Hodgkin disease. In all the diseases studied functions of thrombocytes were altered after treatment with various aggregating agents (ADP, thrombin, collagen, adrenaline, ristomycin).

[Thrombocyte purine nucleoside phosphorylase in various blood diseases]. / Purinnukleozidfosforilaza trombotsitov pri razlichnykh zabolevaniiakh krovi.

Activity of purine nucleoside phosphorylase (EC 2.4.2.1) was estimated in thrombocytes of donors and of patients with various hematological diseases. The enzymatic activity was decreased in chronic lymphoid leukosis chronic myeloleukemia, chronic myeloleukemia and blast transformation, acute lymphoblast and myeloblast leukemia, myeloma. Dissimilar level of purine nucleoside phosphorylase activity was found in non-Hodgkin disease. The activity was only slightly increased in Waldenström's macroglobulinemia; it was similar to normal level in hypoplastic and autoimmune hemolytic anemias. A decrease in the enzymatic activity was observed in thrombocytes of the patients with hereditary forms of hemorrhagic diatheses (hemophilia A, Willebrand disease). Normal level of the enzymatic activity was noted in Glanzmann thrombasthenia.

[Thrombocyte adenase of healthy monkeys and monkeys with different degrees of hemoblastosis]. / Adenaza trombotsitov krovi zdorovykh obez'ian i obez'ian s raznoi vyrazhennost'iu gemoblastoza.

Sensitive radioactive method was used to study the activity of blood platelet adenase in healthy monkeys and in monkeys with hemoblastosis of different severity. A considerable increase of the specific activity of the enzyme in hemoblastosis with an expressed clinical picture and a reduction of the enzymatic activity at the stage of remission and in mild form of the disease was demonstrated. No adenase was revealed in the platelets of healthy monkeys.

[Adenase inhibitor in thrombocytes of healthy humans]. / Ingibitor adenazy trombotsitov zdorovykh liudei.

An inhibitor of adenase, which is absent in leukemic patients, was studied in thrombocytes of healthy persons. The inhibitor, localized in the granular fraction, was isolated by ultracentrifugation in sucrose gradient.

[Adenase of formed elements of blood of donors and of patients with different blood diseases]. / Adenaza formennykh elementov krovi donorov i liudei s razlichnymi zabolevaniiami krovi

The adenase activity was determined in erythrocytes, leucocytes and thrombocytes of donors and of patients with chronic lympholeucosis, chronic myeloleucosis and acute leucosis. A decrease in specific activity of the enzyme was observed in erythrocytes of the patients. In the leucocytes the adenase activity was not found both in normal persons and in all the patients examined. It was shown that the adenase is present in thrombocytes of the patients. Complete absence of the adenase activity was noted in the thrombocytes of healthy donors.