ABSTRACT
We present the first molecular-based report on ungulate malaria parasites from water buffalo in Nepal. Fifty-six blood samples were collected from different groups of water buffalo (wild, feral, and domestic) and PCR assays were conducted using Plasmodium spp. cytb specific primers. Two positive cases were detected, one each from feral and domestic individuals. Complete mitochondrial genome sequence (5987â¯bp) was obtained and examined for nucleotide variations. Sequence analysis revealed identity with type II water buffalo malaria parasites, reported previously, with one A to T nucleotide difference at position 5344. Prevalence, as well as possible economic impacts of water buffalo malaria, should be determined on a wider set of samples from buffalo across Nepal.
Subject(s)
Buffaloes , Malaria/veterinary , Plasmodium/isolation & purification , Animals , Cytochromes b/analysis , Female , Malaria/epidemiology , Male , Nepal/epidemiology , Polymerase Chain Reaction/veterinary , PrevalenceABSTRACT
A birth during the day by a capped langur (Trachypithecus pileatus Blyth, 1843) was recorded at Pakhui Wildlife Sanctuary, Arunachal Pradesh, India. The birth took 43 min. Allomothering was observed 3 h after the birth. An average of 9% of daily active time was shared by four allomothers (three adults, one subadult) during the first 15 days of the infant's life. Total time allomothering was proportional to the age of the allomothers (241 min for oldest; 214 min for youngest).
