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1.
J Mol Microbiol Biotechnol ; 24(4): 249-61, 2014.
Article in English | MEDLINE | ID: mdl-25228469

ABSTRACT

AIM: To gain a greater understanding of the ecology and metabolic potential of the rumen microbiome with the changes in the animal diet. METHODS: Diet composed of varying proportion of green and dry roughages along with grains was given to 8 Mehsani buffaloes, and rumen metagenome was sketched using shotgun semiconductor sequencing. RESULTS: In the present study, the Bacteroidetes were found to be dominant at the phyla level and Prevotella at the genus level. The ratio of Firmicutes to Bacteroidetes was found to be higher in the solid fraction as compared to the liquid fraction. In the solid fraction of the dry roughage group, the significant increment (p < 0.05) in Bacteroidetes abundance was observed with increment of roughage concentration. At the genus level, Clostridium significantly increased with the increment in roughage concentration. A comparison of glycoside hydrolase and cellulosome functional genes revealed more glycoside hydrolase 3 encoding genes with higher fiber diet and significant difference in carbohydrate-active enzymes family composition between green and dry roughage groups of the liquid fraction. CONCLUSION: The present study provides a base to understand the modulating behavior of microbiota which can be manipulated to improve livestock nutrient utilization efficiency and for targeting the efficient catabolism of complex carbohydrate molecules as well.


Subject(s)
Bacteria/classification , Bacteria/genetics , Diet/methods , Metagenome , Microbiota , Rumen/microbiology , Animals , Buffaloes , Sequence Analysis, DNA
2.
Vet Res Commun ; 37(1): 29-35, 2013 Mar.
Article in English | MEDLINE | ID: mdl-23054415

ABSTRACT

The methylation of DNA at cytosine residues within CpG dinucleotides is associated with transcriptional repression and is implicated in maintaining genomic stability and also the silencing of repetitive elements. These imprinted genes are unique as they are expressed exclusively from one parental allele. The present study was carried out to detect methylation status in H19 gene promoter CTCF III region in three Indian buffalo breeds (Jaffarabadi, Surti and Mehsani) by bisulfite sequencing. Methylation percent in Jaffarabadi, Surti and Mehsani buffaloes were found to be 50.19, 70.85 and 52.24, respectively, with mean incidence of methylation percent in H19 in all three breeds as 57.36. Apart from CpG methylation, unexpected nucleotide conversion (T>C, A>G, G>A) and deletion (A and G) after bisulfite sequencing were also observed. We observed no significant relationships in milk yield and milk fat per cent with methylation pattern in H19 gene in any of the three breeds.


Subject(s)
Buffaloes/physiology , DNA Methylation , Milk/chemistry , Milk/metabolism , RNA, Long Noncoding/metabolism , Animals , Buffaloes/genetics , CCCTC-Binding Factor , Cloning, Molecular , CpG Islands , Lactation , Polymerase Chain Reaction/veterinary , Promoter Regions, Genetic , RNA, Long Noncoding/genetics , Repressor Proteins/genetics , Repressor Proteins/metabolism , Sequence Analysis, DNA/veterinary , Sulfites/chemistry
3.
Anim Genet ; 44(4): 442-5, 2013 Aug.
Article in English | MEDLINE | ID: mdl-23216283

ABSTRACT

Evaluations of genetic diversity in domestic livestock populations are necessary to implement region-specific conservation measures. We determined the genetic diversity and evolutionary relationships among eight geographically and phenotypically diverse cattle breeds indigenous to west-central India by genotyping these animals for 22 microsatellite loci. A total of 326 alleles were detected, and the expected heterozygosity ranged from 0.614 (Kenkatha) to 0.701 (Dangi). The mean number of alleles among the cattle breeds ranged from 7.182 (Khillar) to 9.409 (Gaolao). There were abundant genetic variations displayed within breeds, and the genetic differentiation was also high between the Indian cattle breeds, which displayed 15.9% of the total genetic differentiation among the different breeds. The genetic differentiation (pairwise FST ) among the eight Indian breeds varied from 0.0126 for the Kankrej-Malvi pair to 0.2667 for Khillar-Kenkatha pair. The phylogeny, principal components analysis, and structure analysis further supported close grouping of Kankrej, Malvi, Nimari and Gir; Gaolao and Kenkatha, whereas Dangi and Khillar remained at distance from other breeds.


Subject(s)
Cattle/genetics , Genetic Variation , Genetics, Population , Microsatellite Repeats/genetics , Alleles , Animals , Bayes Theorem , Breeding , Cattle/classification , Cluster Analysis , Genetic Markers , Genotype , Geography , India , Phenotype , Phylogeny , Principal Component Analysis , Species Specificity
4.
Am J Hum Genet ; 79(3): 580-5, 2006 Sep.
Article in English | MEDLINE | ID: mdl-16909398

ABSTRACT

Nonsyndromic cleft lip with or without cleft palate (CL-P) is a common congenital anomaly with incidence ranging from 1 in 300 to 1 in 2,500 live births. We analyzed two Indian pedigrees (UR017 and UR019) with isolated, nonsyndromic CL-P, in which the anomaly segregates as an autosomal dominant trait. The phenotype was variable, ranging from unilateral to bilateral CL-P. A genomewide linkage scan that used approximately 10,000 SNPs was performed. Nonparametric linkage (NPL) analysis identified 11 genomic regions (NPL>3.5; P<.005) that could potentially harbor CL-P susceptibility variations. Among those, the most significant evidence was for chromosome 13q33.1-34 at marker rs1830756 (NPL=5.57; P=.00024). This was also supported by parametric linkage; MOD score (LOD scores maximized over genetic model parameters) analysis favored an autosomal dominant model. The maximum LOD score was 4.45, and heterogeneity LOD was 4.45 (alpha =100%). Haplotype analysis with informative crossovers enabled the mapping of the CL-P locus to a region of approximately 20.17 cM (7.42 Mb) between SNPs rs951095 and rs726455. Thus, we have identified a novel genomic region on 13q33.1-34 that harbors a high-risk variant for CL-P in these Indian families.


Subject(s)
Chromosomes, Human, Pair 13/genetics , Cleft Lip/genetics , Cleft Palate/genetics , Genetic Linkage , Genome, Human , Haplotypes , Humans , India , Pedigree , Physical Chromosome Mapping
5.
Am J Med Genet A ; 140(13): 1384-95, 2006 Jul 01.
Article in English | MEDLINE | ID: mdl-16691619

ABSTRACT

Split-hand/split-foot malformation (SHFM, also called ectrodactyly) is a clinically variable and genetically heterogeneous group of limb malformations. Several SHFM loci have been mapped, including SHFM1 (7q21), SHFM2 (Xq26), SHFM3 (10q24), SHFM4 (3q27) and SHFM5 (2q31). To date, mutations in a gene (TP63) have only been identified for SHFM4. SHFM3 has been shown by pulsed-field gel electrophoresis to be caused by an approximately 500 kb DNA rearrangement at 10q24. This region contains a number of candidate genes for SHFM3, though which gene(s) is (are) involved in the pathogenesis of SHFM3 is not known. Our aim in this study was to improve the diagnosis of SHFM3, and to begin to understand which genes are involved in SHFM3. Here we show, using two different techniques, FISH and quantitative PCR that SHFM3 is caused by a minimal 325 kb duplication containing only two genes (BTRC and POLL). The data presented provide improved methods for diagnosis and begin to elucidate the pathogenic mechanism of SHFM3. Expression analysis of 13 candidate genes within and flanking the duplicated region shows that BTRC (present in three copies) and SUFU (present in two copies) are overexpressed in SHFM3 patients compared to controls. Our data suggest that SHFM3 may be caused by overexpression of BTRC and SUFU, both of which are involved in beta-catenin signalling.


Subject(s)
Chromosomes, Human, Pair 10/genetics , F-Box Proteins/genetics , Foot Deformities, Congenital/genetics , Hand Deformities, Congenital/genetics , In Situ Hybridization, Fluorescence/methods , Polymerase Chain Reaction/methods , Female , Foot Deformities, Congenital/epidemiology , Gene Duplication , Hand Deformities, Congenital/epidemiology , Humans , India/epidemiology , Male , Pedigree , Repressor Proteins/genetics , Switzerland/epidemiology , beta-Transducin Repeat-Containing Proteins/genetics
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