A Study of Zn-Ca Nanocomposites and Their Antibacterial Properties.

This study aimed to develop Ca2+ doped ZnO nanoparticles (NPs) and investigate their antibacterial properties against microorganisms of dental interest. Zn-Ca NPs were synthesized by the sol-gel method with different concentrations of Ca2+ (1, 3, and 5 wt. %) and subsequently characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), UV-vis spectroscopy and Fourier transform infrared spectroscopy (FT-IR). The Kirby-Bauer method was used to measure antibacterial effects. NPs showed the wurzite phase of ZnO and bandgap energies (Eg) from 2.99 to 3.04 eV. SEM analysis showed an average particle size of 80 to 160 nm. The treatments that presented the best antibacterial activity were Zn-Ca 3% and Zn-Ca 5%. ZnO NPs represent an alternative to generate and improve materials with antibacterial capacity for dental applications.

Neuropatía de fibras pequeñas: una revisión / Small fibre neuropathy: a review

La neuropatía de fibras pequeñas es una afectación del sistema nervioso periférico cuya principal manifestación es un cuadro de dolor neuropático crónico, acompañado generalmente de alteraciones del sistema nervioso autónomo. Esta enfermedad afecta a fibras nerviosas mielinizadas (Adelta) y no mielinizadas (C), de pequeño calibre. Sus causas pueden ser primarias o secundarias a trastornos del metabolismo, infecciones, enfermedades autoinmunes, neurológicas o tóxicos. Los estudios neurofisiológicos habituales suelen ser negativos. El diagnóstico puede realizarse con exploraciones complementarias de difícil acceso desde la atención primaria, como la microneurografía, test con estímulos sensitivos y otras, aunque la prueba más utilizada es la biopsia cutánea. El tratamiento debe dirigirse a las causas y/o a los síntomas neurológicos. Dada la variabilidad de síntomas con los que puede iniciarse, creemos que es importante conocer esta entidad para aumentar la sospecha clínica, orientar el diagnóstico y poder ofrecer la posibilidad de tratamiento

Small fibre neuropathy is a disorder of the peripheral nervous system for which main clinical manifestation is chronic neuropathic pain, often accompanied by alterations of the autonomic nervous system. This disease affects the small diameter myelinated (ADelta) and non-myelinated nerve fibres (C). Its causes are primary or secondary, such as metabolism defects, infection, and autoimmune, neurological or toxic diseases, among others. Routine neurophysiological tests are usually negative. Diagnosis can be made with complementary tests which are difficult to access from Primary Health Care. These include microneurography, and the sensory stimulation test; but the most used technique is the skin biopsy. Treatment is directed at the causes and the neurological symptoms. Due to the variability of symptoms that can be presented, the importance of being aware of this condition is emphasised in order to reach a diagnosis and offer the appropriate treatment

Alexithymia and obesity: controversial findings from a multimethod assessment.

OBJECTIVE: The aim of the study is to assess alexithymia levels in obese patients using a multimethod measurement (TAS-20 and TSIA) to evaluate both possible differences between the two instruments and their relationship with body weight. PATIENTS AND METHODS: 54 obese patients, seeking surgical treatment, were enrolled. They completed a socio-demographic questionnaire, 20-items Toronto Alexithymia Scale and the Toronto Structured Interview for Alexithymia. RESULTS: Data analysis showed a significant positive association between TAS-20 and TSIA total scores (r=.28, p<.05), but only the TSIA score was positively related to body weight (r=.39; p<.001). Multivariable linear regression models showed the predictive effects of TSIA total score (beta=.41; p<.001) and difficulty in identifying feelings (DIF) (beta=.56; p<.001) respectively on weight. CONCLUSIONS: The findings showed a different association between body weight and alexithymia according to the instrument employed to evaluate alexithymia, supporting the importance of a multimethod assessment in some clinical conditions.

[Small fibre neuropathy: A review]. / Neuropatía de fibras pequeñas: una revisión.

Small fibre neuropathy is a disorder of the peripheral nervous system for which main clinical manifestation is chronic neuropathic pain, often accompanied by alterations of the autonomic nervous system. This disease affects the small diameter myelinated (Aδ) and non-myelinated nerve fibres (C). Its causes are primary or secondary, such as metabolism defects, infection, and autoimmune, neurological or toxic diseases, among others. Routine neurophysiological tests are usually negative. Diagnosis can be made with complementary tests which are difficult to access from Primary Health Care. These include microneurography, and the sensory stimulation test; but the most used technique is the skin biopsy. Treatment is directed at the causes and the neurological symptoms. Due to the variability of symptoms that can be presented, the importance of being aware of this condition is emphasised in order to reach a diagnosis and offer the appropriate treatment.

Evaluation of tetracycline in milk following extra-label administration of topical tetracycline for digital dermatitis in dairy cattle.

Digital dermatitis (DD) is a painful infectious foot lesion commonly treated topically with extra-label tetracycline. Our objectives were to determine the concentrations of tetracycline in milk and plasma and to calculate a withdrawal interval following topical application at various doses. Another objective was to evaluate agreement between tests for measuring tetracycline in milk. A randomized block trial was conducted on 2 farms, where 50 cows with active DD lesions on 2 feet were allocated to 1 of 5 treatment groups (n = 10 cows per group). Treatment groups consisted of topical applications of tetracycline hydrochloride, in a paste or as a powdered form under a bandage, at 3 different dosing levels (2, 5, and 25 g) on each of the 2 affected feet. Following enrollment and treatment, samples were collected from milk, teat skin, and blood every 8 to 24 h for up to 7 d postdosing. Concentrations of tetracycline were measured by liquid chromatography-mass spectrometry and milk samples were further tested using the Charm ROSA TET test (Charm Sciences Inc., Lawrence, MA). Tetracycline was present in milk, plasma, and teat skin from all treatment groups. Tetracycline concentrations varied depending on time of sampling, method of application, and dosing level. At 8 h post-treatment, 11% of cows had tetracycline present in milk higher than 100 ng/mL (ppb) but none higher than 300 ng/mL. The 25-g treatment group had the longest estimated withdrawal interval, the highest observed concentrations (210-244 ng/mL) of tetracycline present in milk, and the longest observed consecutive period of tetracycline presence (from 8 to 72 h) among all treatment groups. Compared with liquid chromatography-mass spectrometry, the Charm test had a sensitivity of 77 and 100% for measuring tetracycline in milk at ≥30 and ≥100 ng/mL, respectively. Post-treatment samples of the teat skin were taken from 15 cows on 6 occasions, and every cow had tetracycline present in at least 1 of those 6 samples. This confirms an association between topical DD treatment with tetracycline and contamination of the teat. A total of 22% of blood samples had detectable tetracycline, and the majority (63%) occurred at 8 h post-treatment. At 100 ng/mL, the estimated cow-level milk withdrawal interval ranged from 0 to 70 h. At 300 ng/mL, the estimated cow-level withdrawal interval ranged from 0 to 34 h, and was 0 h at the bulk tank level. We recommend that conservative measures be adopted after extra-label use of topical tetracycline for DD treatment, including using a low dose and strategic post-treatment testing for tetracycline-class drugs in milk.

Contra-réplica a "Visibilidad de los institutos de investigación sanitaria a través de la base de datos Web of Science" / Rejoinder to "Visibility of healthcare research institutes through the Web of Science database"

No disponible

Evaluation of agreement among digital dermatitis scoring methods in the milking parlor, pen, and hoof trimming chute.

Digital dermatitis (DD) is the most common infectious foot lesion affecting welfare and productivity of dairy cattle. The key to DD control programs is routine and frequent identification of DD lesions. The objective was to evaluate accuracy of detecting and scoring DD lesions in 3 milking parlor designs and in 3 alternative settings compared with scoring in the hoof trimming chute as reference. A total of 552 cows and 1,104 hind feet from 17 freestall farms were scored by 1 observer in the milking parlor and in 1 other setting: pen, headlocks, or management rail. After being scored in the milking parlor and at least 1 other setting, cows were examined in the hoof trimming chute, considered the gold standard. In every setting, all hind feet were inspected visually using a flashlight and without prior washing of feet. Agreement of the scoring settings was assessed using the 5 M-stage scoring system and a dichotomous absence (M0 or M0/M1) or presence (M1 to M4.1 or M2 to M4.1) system. At trimming chute inspection, 44% of feet had a DD lesion, with estimates of 11, 5, 2, 10, and 16% for M1, M2, M3, M4, and M4.1 lesions, respectively. Apparent DD foot-level prevalence at the milking parlor, pen, management rail, and headlocks was 28, 22, 16, and 22%, respectively. M-stages were less discernible in the pen, management rail, and headlocks (apparent prevalence of M1, M2, M3, and M4.1 was ≤1%) compared with the trimming chute and milking parlor. Agreement beyond chance between any scoring setting and trimming chute scoring ranged from 0.48 to 0.70 for the dichotomous scoring system (absence = M0/M1 vs. presence = M2 to M4.1). Diagnostic test performance varied greatly among DD scoring settings but, in general, it had low sensitivity (<70%) and high specificity (>93%) for detecting any DD lesion. Agreement and test characteristics were not affected by the type of milking parlor. Although the milking parlor and headlocks were the most reliable settings in which to detect DD, none of the settings were reliable enough to replace inspection of feet in the trimming chute. However, scoring the presence or absence of DD in the milking parlor, pen, management rail, and headlocks could be used to estimate within-herd DD prevalence, to improve DD surveillance through routine monitoring, and to evaluate effects of interventions at the farm level.

Visibilidad de los institutos de investigación sanitaria a través de la base de datos Web of Science / Visibility of healthcare research institutes through the Web of Science database

Objetivos. La importancia estratégica de los institutos de investigación sanitaria (IIS) en la investigación en ciencias de la salud en España motiva este análisis sobre la viabilidad de estudiar su contribución a la producción científica española a través de su presencia como institución firmante en las publicaciones. Material y métodos. Se identifica la producción de los IIS en la base de datos Web of Science comparando su producción observada (el instituto figura de forma explícita en el lugar de trabajo de los autores) y potencial (estimada en función de los hospitales vinculados). Resultados y conclusiones. Los estudios basados en publicaciones científicas no permiten identificar de forma fiable la contribución de los IIS, ya que su producción observada es muy inferior a la potencial, aunque su visibilidad tiende a aumentar a lo largo del tiempo. Se señala el interés de que los miembros de los IIS incluyan al instituto entre sus direcciones de trabajo para aumentar la visibilidad de estas estructuras y posibilitar estudios orientados a valorar su actividad en el contexto nacional e internacional (AU)

Objectives. The strategic importance of healthcare research institutes (HRIs) in health sciences research in Spain has motivated this analysis of the feasibility of studing their contribution to the Spanish scientific output through their presence as a signatory institution in the publications. Material and methods. We identified the output of the HRIs in the Web of Science database, comparing their observed output (the institutes are explicitly listed in the authors’ workplace) and potential output (estimated based on the linked hospitals). Results and conclusions. The studies based on scientific publications do not help us reliably identify the contribution of the HRIs because their observed production is much lower than the potential output, although their visibility tends to increase over time. This article highlights the importance of HRI members including the institute among their work addresses to increase the visibility of these organisations and to facilitate studies aimed at assessing their activity in the national and international context (AU)

Producer estimates of prevalence and perceived importance of lameness in dairy herds with tiestalls, freestalls, and automated milking systems.

Lameness is one of the most important welfare and productivity concerns in the dairy industry. Our objectives were to obtain producers' estimates of its prevalence and their perceptions of lameness, and to investigate how producers monitor lameness in tiestall (TS), freestall with milking parlor (FS), and automated milking system (AMS) herds. Forty focal cows per farm in 237 Canadian dairy herds were scored for lameness by trained researchers. On the same day, the producers completed a questionnaire. Mean herd-level prevalence of lameness estimated by producers was 9.0% (±0.9%; ±SE), whereas the researchers observed a mean prevalence of 22.2% (±0.9%). Correlation between producer- and researcher-estimated lameness prevalence was low (r = 0.19) and mean researcher prevalence was 1.6, 1.8, and 4.1 times higher in AMS, FS, and TS farms, respectively. A total of 48% of producers thought lameness was a moderate or major problem in their herds (TS = 34%; AMS =53%; FS = 59%). One third of producers considered lameness the highest ranked health problem they were trying to control, whereas two-thirds of producers (TS = 43%; AMS = 63%; FS = 71%) stated that they had made management changes to deal with lameness in the past 2 yr. Almost all producers (98%) stated they routinely check cows to identify new cases of lameness; however, 40% of producers did not keep records of lameness (AMS = 24%; FS = 23%; TS = 60%). A majority (69%) of producers treated lame cows themselves immediately after detection, whereas 13% relied on hoof-trimmer or veterinarians to plan treatment. Producers are aware of lameness as an issue in dairy herds and almost all monitor lameness as part of their daily routine. However, producers underestimate lameness prevalence, which highlights that lameness detection continues to be difficult in in all housing systems, especially in TS herds. Training to improve detection, record keeping, identification of farm-specific risk factors, and treatment planning for lame cows is likely to help decrease lameness prevalence.

Visibility of healthcare research institutes through the Web of Science database. / Visibilidad de los institutos de investigación sanitaria a través de la base de datos Web of Science.

OBJECTIVES: The strategic importance of healthcare research institutes (HRIs) in health sciences research in Spain has motivated this analysis of the feasibility of studing their contribution to the Spanish scientific output through their presence as a signatory institution in the publications. MATERIAL AND METHODS: We identified the output of the HRIs in the Web of Science database, comparing their observed output (the institutes are explicitly listed in the authors' workplace) and potential output (estimated based on the linked hospitals). RESULTS AND CONCLUSIONS: The studies based on scientific publications do not help us reliably identify the contribution of the HRIs because their observed production is much lower than the potential output, although their visibility tends to increase over time. This article highlights the importance of HRI members including the institute among their work addresses to increase the visibility of these organisations and to facilitate studies aimed at assessing their activity in the national and international context.

Immediate effects of thoracic spinal mobilisation on erector spinae muscle activity and pain in patients with thoracic spine pain: a preliminary randomised controlled trial.

OBJECTIVES: To investigate the activity of the thoracic erector spinae muscles and perceived pain intensity immediately after central postero-anterior (PA) mobilisation of the thoracic spine. DESIGN: Randomised, placebo-controlled, experimental design. PARTICIPANTS AND INTERVENTIONS: Thirty-four participants with non-specific thoracic pain were randomised to the experimental group [grade III central PA mobilisation performed for 3minutes at the level of the seventh thoracic vertebra (T7)] or the placebo group (less than grade I central PA mobilisation performed for 3minutes at T7). MAIN OUTCOME MEASURES: Before and immediately after PA mobilisation, surface electromyography (EMG) was recorded from the thoracic erector spinae muscles as the participants performed 10° spine extension from a prone position for 10seconds. Each participant rated their pain intensity as an investigator performed grade III central PA over the most symptomatic thoracic segment, and the pressure pain threshold (PPT) was evaluated bilaterally over the erector spinae muscles. RESULTS: The EMG amplitude of thoracic erector spinae activity was reduced significantly after the intervention in the experimental group (P<0.05), but not in the placebo group. The difference between the groups was significant {pre-post change: placebo -14 [standard deviation (SD) 50]mV, experimental 28 (SD 48)mV; mean difference -42mV; 95% confidence interval of the difference -76 to 7; P<0.05} albeit small (Grissom=0.44). However, both groups showed a significant reduction in pain immediately after the intervention, and both groups showed a similar pre-post change in PPT. CONCLUSION: These preliminary findings indicate that grade III central mobilisation over the most symptomatic thoracic segment reduces thoracic erector spinae activity during extension of the trunk in people with non-specific thoracic spine pain. CLINICAL TRIAL REGISTRATION NUMBER: ISRCTN47601528.

Effectiveness of a standardized footbath protocol for prevention of digital dermatitis.

A footbath is the most common herd-level approach to control digital dermatitis (DD) in intensive dairy farming. However, wide variation in footbath design and protocols suggests a gap between evidence-based management practices and on-farm implementation. The objective was to evaluate effectiveness of a standardized footbath protocol in decreasing prevalence of active DD lesions in lactating dairy cattle. The protocol was based on current scientific literature, including footbath design and management. The M-stage classification system was used to score DD lesions. Scores were also combined into a simplified scoring system: no lesions (M0), active lesions (M1, M2, and M4.1), and chronic lesions (M3 and M4). A controlled intervention trial was conducted on 9 farms over 22 wk. Each farm served as its own control with data collected for 10 wk before and 12 wk after intervention. A total of 1,978 lactating cattle were assessed biweekly for DD lesions and leg cleanliness in the milking parlor. Lactating cattle were also inspected in the trimming chute at 3 time points: start, intervention, and end of trial. Intervention consisted of implementing an automated footbath that measured 3 m long, 0.50 m wide, held a fluid depth of 0.15 m, along with a weekly footbath protocol using 5% CuSO4 for 4 consecutive milkings, with footbath content replaced at a maximum of 200 cow passes. Multilevel logistic regression models for repeated measures were used to evaluate effects of the standardized footbath protocol in preventing active DD lesions. For the purpose of analysis, farms' within-herd prevalence of active DD lesions (at baseline) was assessed and categorized as low (<15%) or high (≥15%). Farms with low or high within-herd prevalence of active DD lesions at trial outset had a mean cow-level prevalence of active DD lesions of 8% (range, 2 to 13) and 31% (range, 18 to 43), respectively. At milking parlor inspections, apparent prevalence of active DD lesions decreased from the time of footbath intervention, but this effect interacted with the farms' baseline prevalence of active DD lesions. In that regard, on farms with high prevalence of active DD at baseline, apparent prevalence of active DD lesions decreased after intervention, whereas on farms with low prevalence of active DD at baseline, apparent prevalence of active DD lesions did not change. At the cow level, poor leg cleanliness was associated with higher prevalence of active DD lesions. At trimming chute inspections, prevalence of active DD lesions decreased from start to the end of the trial (22 and 14%, respectively); concurrently, prevalence of feet with no DD lesions (M0) increased (39 and 48%). We concluded that on farms with high DD prevalence, implementation of proper footbath design and improvement of footbathing management will decrease prevalence of active DD lesions and increase prevalence of feet without DD lesions. In addition, improving cow cleanliness will further result in control of active DD lesions.

Validation of the M-stage scoring system for digital dermatitis on dairy cows in the milking parlor.

A high prevalence of digital dermatitis (DD) and the benefits of early topical treatment highlight the need for simple tools for routine DD detection. The objective of this study was to determine the accuracy of scoring DD lesions using the 5 M-stage scoring system in the milking parlor compared with the trimming chute as the gold standard. Three observers inspected 3,585 cows and 6,991 hind feet from 9 farms in the milking parlor using a mirror (glued to a plastic kitchen spatula) and a headlamp, followed by inspection in a trimming chute within 5 d. Interobserver agreement for scoring DD in various settings was ≥82% (kappa >0.74; weighted kappa >0.76). At trimming chute inspections, 68% of cows had at least 1 DD lesion, 19% had 1 hind leg affected, and 49% had both hind legs affected. Within-herd DD prevalence ranged from 16 to 81% of cows affected. True within-herd prevalence was 2, 6, 0, 36, and 14% for M1, M2, M3, M4, and M4.1 lesions, respectively. At the foot level, DD prevalence was the same (58%) in the milking parlor and trimming chute inspection, but distribution of M-stages differed. Milking parlor inspection as a means of identifying the presence of DD lesions had a sensitivity of 92% and specificity of 88%, with positive and negative predictive values of 91 and 89%, respectively. Agreement between milking parlor and trimming chute inspections was 73% (kappa = 0.59, weighted kappa = 0.65) for the 5 M-stage scoring system and 90% (kappa = 0.80) if only the presence of a lesion was noted. Test characteristics varied greatly among M-stages, with the highest sensitivity for detecting M4 (82%) and M2 (62%) lesions, and the lowest for detecting M4.1 (20%), M1 (7%), and M3 (0%) lesions. In the milking parlor, 20% of M2 lesions were misclassified as M4.1, 8% of M4 lesions were misclassified as M0, and 68% of M4.1 lesions were misclassified as M4. The majority (87%) of DD lesions were located between the heel bulbs; 10 and 2% of DD lesions affected the interdigital space and the front of the foot, respectively. The sensitivity to detect the presence of a lesion when it occurred between the heel bulbs was 93%, but <67% if it occurred elsewhere on the foot. We concluded that inspection of the rear feet in the milking parlor was an inexpensive and simple method of detecting and scoring DD lesions. If the objective is to determine herd-level DD prevalence and routine monitoring, this method was adequately reliable. However, if the objective is to follow up DD in cows with history of interdigital hyperplasia or to detect M1 or M4.1 lesions, this method was not sufficiently reliable. Although DD scoring in the milking parlor as a routine practice should facilitate early detection, prompt treatment interventions, and herd monitoring, it was not sufficiently reliable to replace definitive identification of M-stages in the trimming chute.

Prevalence and distribution of foot lesions in dairy cattle in Alberta, Canada.

The objectives of this cross-sectional study were to determine the prevalence and distribution of foot lesions and associated cow- and herd-level risk factors in dairy cows in Alberta, Canada. Foot lesion data were recorded electronically by 7 hoof trimmers on 28,607 cows in 156 dairy farms from June 2009 to November 2012. Foot lesion prevalence estimates differed between farms that had the whole herd trimmed at once (≥80% of lactating cows were trimmed; n=69 farms and 8,020 cows) and farms on which part of the herd was trimmed (selection of cows was determined by farmer and <80% of lactating cows were trimmed; n=87 and 20,587 cows). Estimates were consistently higher for the latter likely because farmers presumably prioritized lame cows in partial-herd trims. On farms with whole-herd trims, digital dermatitis was the most common lesion among all housing types, present in 15% of cows and 94% of herds. Sole ulcers and white line disease were detected in 6 and 4% of the cows and 92 and 93% of herds, respectively. Other infectious and claw horn lesions each affected 1 to 2% of cows and 62 to 78% of herds. Intraclass correlation coefficients for hoof trimmers ranged from 0.01 to 0.20 for all lesions, indicating some clustering of recorded lesions by trimmer. Multilevel mixed logistic regression models were constructed (including hoof trimmer as fixed and farm as random effects) for the 3 most frequently identified lesions. Prevalence of digital dermatitis decreased with increasing parity, but this effect interacted with days in milk (DIM); primiparous cows had higher odds of digital dermatitis in mid lactation (100-199 DIM) and late lactation (≥200 DIM) compared with cows at other stages of lactation. In contrast, prevalence of sole ulcers and white line disease increased with increasing parity; compared with cows in parity 1, those in parity 4 had 5 or 7 times higher odds of having these lesions, respectively. Cows in mid lactation and late lactation had higher odds of sole ulcers and white line disease than cows at other stages of lactation, regardless of parity. Digital dermatitis prevalence was 2 times higher in herds housed in barns with access to an exercise area. The odds of sole ulcers and white line disease were ≥2 times higher in cows housed in freestalls than those housed in deep-bedded packs. Therefore, preventive measures for control of digital dermatitis merit emphasis, especially in primiparous cows and on farms with exercise areas. In addition, improving housing environment by providing a deep-bedded area for older cows in mid lactation or late lactation could reduce prevalence of claw horn lesions. We inferred that foot lesion data recorded by hoof trimmers can provide useful information not only to develop effective foot health programs at herd level, but also for disease surveillance and genetic improvement at regional and national levels.

Associations between lying behavior and lameness in Canadian Holstein-Friesian cows housed in freestall barns.

Lying behavior is an important measure of comfort and well-being in dairy cattle, and changes in lying behavior are potential indicators and predictors of lameness. Our objectives were to determine individual and herd-level risk factors associated with measures of lying behavior, and to evaluate whether automated measures of lying behavior can be used to detect lameness. A purposive sample of 40 Holstein cows was selected from each of 141 dairy farms in Alberta, Ontario, and Québec. Lying behavior of 5,135 cows between 10 and 120 d in milk was automatically and continuously recorded using accelerometers over 4 d. Data on factors hypothesized to influence lying behavior were collected, including information on individual cows, management practices, and facility design. Associations between predictor variables and measures of lying behavior were assessed using generalized linear mixed models, including farm and province as random and fixed effects, respectively. Logistic regression models were used to determine whether lying behavior was associated with lameness. At the cow-level, daily lying time increased with increasing days in milk, but this effect interacted with parity; primiparous cows had more frequent but shorter lying bouts in early lactation, changing to mature-cow patterns of lying behavior (fewer and longer lying bouts) in late lactation. In barns with stall curbs >22 cm high, the use of sand or >2 cm of bedding was associated with an increased average daily lying time of 1.44 and 0.06 h/d, respectively. Feed alleys ≥ 350 cm wide or stalls ≥ 114 cm wide were associated with increased daily lying time of 0.39 and 0.33 h/d, respectively, whereas rubber flooring in the feed alley was associated with 0.47 h/d lower average lying time. Lame cows had longer lying times, with fewer, longer, and more variable duration of bouts compared with nonlame cows. In that regard, cows with lying time ≥ 14 h/d, ≤ 5 lying bouts per day, bout duration ≥ 110 min/bout, or standard deviations of bout duration over 4 d ≥ 70 min had 3.7, 1.7, 2.5, and 3.0 higher odds of being lame, respectively. Factors related to comfort of lying and standing surfaces significantly affected lying behavior. Finally, we inferred that automated measures of lying behavior could contribute to lameness detection, especially when interpreted in the context of other factors known to affect lying behavior, including those associated with the individual cow (e.g., parity and stage of lactation) or environment (e.g., stall surface).

Prevalence of lameness and associated risk factors in Canadian Holstein-Friesian cows housed in freestall barns.

Lameness is a severe welfare problem and a production-limiting disease in dairy farming. The objectives of this study were to determine prevalence of lameness and investigate cow- and herd-level factors associated with lameness in dairy cows housed in freestall barns in 3 Canadian provinces. A purposive sample of 40 Holstein-Friesian cows was selected from each of 141 dairy farms in Québec, Ontario, and Alberta. In total, 5,637 cows were scored once for lameness (presence of limping when walking). Data collected included information on individual cows (hock lesions, claw length, body condition score, parity, days in milk, and milk production), management practices (floor and stall cleaning routine, bedding routine, and footbath practices), and facility design (stall dimensions, stall base and bedding type, width of feed alley, flooring type, and slipperiness) hypothesized to be risk factors for lameness. Multilevel mixed logistic regression models were constructed (including farm as a random effect and province as a fixed effect). Herd-level lameness prevalence ranged from 0 to 69% (mean = 21%). Lameness prevalence increased with increasing parity; compared with first parity, cows in parity 2, 3, and ≥ 4 had 1.6, 3.3, and 4 times, respectively, higher odds of being lame. Furthermore, the odds of lameness were 1.6 times greater in cows with low body condition score (≤ 2.5) than in cows with a higher body condition score. In addition, injured hocks and overgrown claws were associated with 1.4- and 1.7-fold increased odds of being lame, respectively, whereas every 1 kg increase in daily milk production was associated with a 3% decrease in the odds of being lame. Lameness prevalence was higher in herds with ≤ 100 cows, but lower in barns with a sand or dirt stall base, or with bedding ≥ 2 cm deep. Cows exposed to very slippery floors had 2 times the odds of being lame compared with cows exposed to nonslippery floors. We attributed the wide range of lameness prevalence to the great variability in facilities and management practices among farms. Finally, we inferred that the prevalence of lameness could be decreased by improving management of multiparous, thin, or injured cows and by adopting management practices intended to improve cow comfort, namely the floor's slip resistance and the stall's lying surface.

Catalytic aerobic oxidation of phenols to ortho-quinones with air-stable copper precatalysts.

A range of air-stable copper species was examined for catalytic activity in the catalytic aerobic transformation of phenols into ortho-quinones. Efficient catalysis was obtained with commercially available copper(II) acetate. The stability of all constituents before mixing makes for a practical process that advances previously reported copper(I)-based oxygenations.

[Pneumocystis jiroveci pneumonia: Clinical characteristics and mortality risk factors in an Intensive Care Unit]. / Neumonía por Pneumocystis jiroveci: características clínicas y factores de riesgo asociados a mortalidad en una Unidad de Cuidados Intensivos.

OBJECTIVE: To describe the epidemiological characteristics of the population with Pneumocystis jiroveci (P. jiroveci) pneumonia, analyzing risk factors associated with the disease, predisposing factors for admission to an intensive care unit (ICU), and prognostic factors of mortality. DESIGN AND PATIENTS: A retrospective observational study was carried out, involving a cohort of patients consecutively admitted to a hospital in Spain from 1 January 2007 to 31 December 2011, with a final diagnosis of P. jiroveci pneumonia. SETTING: The ICU and hospitalization service of Hospital del Mar, Barcelona (Spain). RESULTS: We included 36 patients with pneumonia due to P. jiroveci. Of these subjects, 16 required ICU admission (44.4%). The average age of the patients was 41.3 ± 12 years, and 23 were men (63.9%). A total of 86.1% had a history of human immunodeficiency virus (HIV) infection, and the remaining 13.9% presented immune-based disease subjected to immunosuppressive therapy. Risk factors associated to hospital mortality were age (51.8 vs. 37.3 years, P=.002), a higher APACHE score upon admission (17 vs. 13 points, P=.009), the need for invasive mechanical ventilation (27.8% vs. 11.1%, P=.000), requirement of vasoactive drugs (25.0% vs. 11.1%, P=.000), fungal coinfection (22.2% vs. 11.1%, P=.001), pneumothorax (16.7% vs. 83.3%, P=.000) and admission to the ICU (27.8% vs. 72.2% P=.000). CONCLUSIONS: The high requirement of mechanical ventilation and vasoactive drugs associated with fungal coinfection and pneumothorax in patients admitted to the ICU remain as risk factors associated with mortality in patients with P. jiroveci pneumonia.

Neumonía por pneumocystis jiroveci: características clínicas y factores de riesgo asociados a mortalidad en una unidad de cuidados intensivos / Pneumocystis jiroveci pneumonia: clinical characteristics and mortality risk factorsinanintensive care unit

Objetivo. Describir las características epidemiológicas de la población con neumonía por Pneumocystis jiroveci (P. jiroveci), analizando factores de riesgo asociados a la enfermedad, factores predisponentes para ingreso en una unidad de cuidados intensivos (UCI) y factores pronóstico de mortalidad. Diseño y pacientes Estudio observacional y retrospectivo de una cohorte de pacientes ingresados consecutivamente en un hospital de España desde el 1 de enero del 2007 al 31 de diciembre del 2011 cuyo diagnóstico final fue neumonía por P. jiroveci.ÁmbitoUCI y servicio de hospitalización del Hospital del Mar, Barcelona. Resultados. Se incluyeron 36 pacientes con neumonía por P. jiroveci de los que 16 (44,4%) precisaron de ingreso en UCI. La edad media de todos los pacientes fue de 41,3±12 años, de los que 23 (63,9%) eran hombres. El 86,1% tenía antecedente de infección por virus de la inmunodeficiencia humana (VIH) y el resto (el 13,9%) de alguna enfermedad inmunológica con tratamiento inmunosupresor. Los factores de riesgo asociados a mortalidad hospitalaria fueron la edad (51,8 vs. 37,3 años; p=0,002), APACHE mayor al ingreso (17 vs. 13 puntos; p=0,009), la necesidad de ventilación mecánica invasiva (27,8 vs. 11,1%; p=0,000), requerimiento de fármacos vasoactivos (25 vs. 11,1%; p=0,000), coinfección fúngica (22,2 vs. 11,1%; p=0,001), neumotórax (16,7 vs. 83,3%; p=0,000) ingreso en UCI (27,8 vs. 72,2%; p=0,000). Conclusiones. Los altos requerimientos de ventilación mecánica y de fármacos vasoactivos asociado a la coinfección fúngica y presencia de neumotórax en pacientes ingresados en la UCI continúan siendo factores de riesgo asociados a mayor mortalidad en pacientes con neumonía por P. jiroveci.