Inoculation with Native Actinobacteria May Improve Desert Plant Growth and Survival with Potential Use for Restoration Practices.

Soil microorganisms, together with water, play a key role in arid ecosystems, being responsible for the nutrient cycle, facilitating nutrient incorporation into plants, influencing plant drought tolerance, and enhancing their establishment. Therefore, their use for restoration practices is promising. We tested the potential of native strains of Actinobacteria from Monte Desert as growth promoters of native vegetation, isolating them from two substrates from their habitat (bare soil and leaf-cutting ant refuse dumps). Strains were inoculated into the soil where seedlings of three native plant species (Atriplex lampa, Grindelia chiloensis, Gutierrezia solbrigii) were growing. Seedlings were grown following a full factorial design experiment under greenhouse and field conditions comparing native Actinobacteria effects with a known growth-promoting strain, Streptomyces sp. (BCRU-MM40 GenBank accession number: FJ771041), and control treatments. Seedlings survived greenhouse condition but species survival and growth were different among treatments at field conditions, varying over time. The highest survival was observed in a native soil strain (S20) while the lowest in MM40. The low survival in MM40 and in the other treatments may be explained by the higher herbivory observed in those seedlings compared to control ones, suggesting a higher nutritional status in inoculated plants. Strains from refuse dumps were the best at enhancing seedling growth, while strains from soil were the best at maintaining their survival. Native Actinobacteria studied may increase plant species survival and growth by improving their nutritional status, suggesting their potential to facilitate vegetation establishment and, therefore, being good candidates for restoration practices. Furthermore, plant species respond differently to different strains, highlighting the importance of microorganism diversity for ecosystem functioning.

A systematic review and meta-analysis of the 2007 WCRF/AICR score in relation to cancer-related health outcomes.

BACKGROUND: We conducted a systematic literature review and meta-analysis of observational studies investigating adherence to the 2007 World Cancer Research Fund/American Institute for Cancer Research (WCRF/AICR) lifestyle recommendations for cancer prevention and health outcomes. PATIENTS AND METHODS: We searched PubMed and the in-house database of the WCRF Continuous Update Project for publications up to June 2019. Cross-sectional studies were only narratively reviewed given their heterogeneity while findings of cohort/case-control studies were synthesized in umbrella reviews and meta-analyses. Summary relative risks (RRs) and 95% confidence intervals (CI) were estimated using a random-effects model when at least two studies reported results on a specific outcome. RESULTS: Thirty-eight articles (17 prospective, 8 case-control, and 13 cross-sectional studies) were included. The summary RR per each point increment in the 2007 WCRF/AICR score was 0.90 (95% CI: 0.87-0.93, n = 11) for breast cancer, regardless of hormone receptor and menopausal status, 0.86 (95% CI: 0.82-0.89, n = 10) for colorectal cancer, and 0.93 (95% CI: 0.89-0.96, n = 2) for lung cancer risk. No statistically significant associations were reported for prostate (n = 6) and pancreatic cancers (n = 2). Adherence to the recommendations was associated with lower overall mortality (RR = 0.90, 95% CI 0.84-0.96, n = 3) and cancer-specific mortality (RR = 0.91, 95% CI 0.89-0.92; n = 3) in healthy populations, as well as with higher survival in cancer patients (n = 2). In cross-sectional studies, a healthier plasma marker profile and lower cancer risk factors in the general population and a better health status and quality of life in cancer patients/survivors were reported. CONCLUSIONS: Adhering to the 2007 WCRF/AICR recommendations is associated with lower risks of cancer incidence, namely breast and colorectal cancers, and mortality. Primary prevention of cancer should emphasize modification of multiple lifestyle factors. Upcoming studies examining the recently updated 2018 guidelines will further clarify such associations.

Faecal bacterial and short-chain fatty acids signature in hypercholesterolemia.

Gut microbiota has been suggested to affect lipid metabolism. The objective of this study was to characterize the faecal microbiota signature and both short chain fatty acids (SCFAs) and bile acids (BA) profile of hypercholesterolemic subjects. Microbiota composition, SCFAs, BA and blood lipid profile from male volunteers with hypercholesterolemia (HC) and normocholesterolemia (NC) were determined by 16S rDNA sequencing, HPLC, GC and NMR, respectively. HC subjects were characterized by having lower relative abundance of Anaeroplasma (0.002% vs 0.219%, p-value = 0.026) and Haemophilus (0.041% vs 0.078%, p-value = 0.049), and higher of Odoribacter (0.51% vs 0.16%; p-value = 0.044). Correlation analysis revealed that Anaeroplasma and Haemophilus were associated to an unfavourable lipid profile: they correlated negatively to cholesterol and triglycerides related biomarkers and the ratio total to high density lipoprotein (HDL) cholesterol, and positively to HDL size. Odoribacter displayed an opposite behaviour. Faecal SCFAs profile revealed higher abundance of isobutyric (2.76% vs 0.82%, p-value = 0.049) and isovaleric acid (1.32% vs 0.06%, p-value = 0.016) in HC. Isobutyric acid correlated positively with Odoribacter and lipid parameters indicative of an unfavourable profile. BA profile did not show differences between groups. It was concluded that HC subjects showed a particular faecal bacterial signature and SCFAs profile associated with their lipid profile.

Compositional analysis of dietary patterns.

Instead of looking at individual nutrients or foods, dietary pattern analysis has emerged as a promising approach to examine the relationship between diet and health outcomes. Despite dietary patterns being compositional (i.e. usually a higher intake of some foods implies that less of other foods are being consumed), compositional data analysis has not yet been applied in this setting. We describe three compositional data analysis approaches (compositional principal component analysis, balances and principal balances) that enable the extraction of dietary patterns by using control subjects from the Spanish multicase-control (MCC-Spain) study. In particular, principal balances overcome the limitations of purely data-driven or investigator-driven methods and present dietary patterns as trade-offs between eating more of some foods and less of others.

Mortality of women with ductal carcinoma in situ of the breast: a population-based study from the Girona province, Spain (1994-2013).

PURPOSE: We aim to comprehensively describe the incidence and mortality trends of ductal carcinoma in situ (DCIS) in the Girona province, Spain (1994-2013) and to estimate the all-cause mortality excess risk of diagnosed women. METHODS: Age-standardized rates of DCIS were estimated between 1994 and 2013. Standard mortality ratios (SMR) and absolute excess mortality were calculated overall and by tumor and patient characteristics. A sensitivity analysis was conducted excluding cases with a subsequent invasive breast cancer (sIBC). RESULTS: Of the 641 women included, 56 died (follow-up time: 8.4 person-years). Between 1994 and 2013, a significant increase in incidence and decrease in mortality was identified among women aged between 50 and 69 years old. Neoplasms and circulatory system disease were the most common causes of death. No excess risk of death was found overall, except for women aged < 50 years (SMR = 3.44, 95% CI 1.85; 6.40) and those with a sIBC (SMR = 2.51, 95% CI 1.26; 5.02), risk that lessened when cases with sIBC were excluded. Patients with sIBC also showed an excess risk (SMR = 2.29, 95% CI 1.03; 5.10). CONCLUSIONS: Among women aged 50-69 years old, incidence of DCIS has significantly increased yet mortality has decreased. Overall, the all-cause mortality risk of women diagnosed with DCIS remains similar to that of the general population except for women diagnosed before age 50 and those with sIBC, who showed a significant increased risk. Differential management of these patients should be considered.

Biarticular total femoral spacer for complete bone loss in periprosthetic joint infection. A technical modification. / Espaciador femoral total biarticulado para el tratamiento de la infección periprotésica de cadera y rodilla con pérdida completa de hueso. Modificación técnica.

BACKGROUND: The management of complete bone defects in hip and knee periprosthetic infection is still a real surgical challenge. MATERIAL AND METHODS: We present a technical modification for performing a biarticular total femoral spacer with a femoral nail without the need to approach the proximal tibia. RESULTS: Three patients were operated with this technique. There was no intraoperative complication. The infection was resolved in all patients operated at final follow-up. All patients improved their previous functional situation and could walk with different aids. CONCLUSIONS: This technical modification is an alternative for cases where it is necessary to resect the complete femur, but it is not necessary to approach the tibia.

Incidence and survival of primary central nervous system lymphoma (PCNSL): results from the Girona cancer registry (1994-2013)

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Childhood and adolescent lymphoma in Spain: incidence and survival trends over 20 years

Background: Lymphoma is the third most common malignancy in children (0-14 years) and the first in adolescents (15-19 years). This population-based study-the largest ever done in Spain-analyses incidence and survival of lymphomas among Spanish children and adolescents. Patients and methods 1664 lymphoma cases (1983-2007) for incidence and 1030 for survival (1991-2005) followed until 31/12/2010, were provided by 11 cancer registries. Age-adjusted incidence rates (ASRw) to the world standard population were obtained; incidence trends were modelled using the Joinpoint programme, observed survival (OS) was estimated with Kaplan-Meier and trends tested with a log-rank test. Results are presented according to the International Classification of Childhood Cancer-3. Results: In Spain, the ASRw0-14 for lymphomas was 17.5 per 1.000.000 child-years and 50.0 the specific rate for adolescents. Overall incidence increased significantly during 1983-1997 with no increases thereafter. Patients over 9 years old showed significant rising trends for all subtypes, except for Burkitt lymphoma (BL) in adolescents. During 2001-2005 (age 0-19 years), 5-year OS was 94 (90-98), 73 (64-83) and 86 (78-94) for Hodgkin lymphoma (HL), non-Hodgkin lymphoma (NHL) and BL, respectively. No improvement in survival was found. The incidence in Spain was higher than overall European rates, but within the range of that in Southern Europe. Comparing OS in Spain 1991-1995 and 2001-2005 with results for Europe of the Automated Childhood Cancer Information System (ACCIS) (1988-1997) and the European cancer registry-based study on survival and care of cancer patients (EUROCARE) (2000-2007), it was similar for HL and lower for NHL and BL. Conclusions: Systematic monitoring and analysis of lymphoma paediatric data would provide clinical and epidemiological information to improve the health care of these patients and the outcomes for these malignancies in Spain

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Childhood and adolescent lymphoma in Spain: incidence and survival trends over 20 years.

BACKGROUND: Lymphoma is the third most common malignancy in children (0-14 years) and the first in adolescents (15-19 years). This population-based study-the largest ever done in Spain-analyses incidence and survival of lymphomas among Spanish children and adolescents. PATIENTS AND METHODS: 1664 lymphoma cases (1983-2007) for incidence and 1030 for survival (1991-2005) followed until 31/12/2010, were provided by 11 cancer registries. Age-adjusted incidence rates (ASRw) to the world standard population were obtained; incidence trends were modelled using the Joinpoint programme, observed survival (OS) was estimated with Kaplan-Meier and trends tested with a log-rank test. Results are presented according to the International Classification of Childhood Cancer-3. RESULTS: In Spain, the ASRw0-14 for lymphomas was 17.5 per 1.000.000 child-years and 50.0 the specific rate for adolescents. Overall incidence increased significantly during 1983-1997 with no increases thereafter. Patients over 9 years old showed significant rising trends for all subtypes, except for Burkitt lymphoma (BL) in adolescents. During 2001-2005 (age 0-19 years), 5-year OS was 94 (90-98), 73 (64-83) and 86 (78-94) for Hodgkin lymphoma (HL), non-Hodgkin lymphoma (NHL) and BL, respectively. No improvement in survival was found. The incidence in Spain was higher than overall European rates, but within the range of that in Southern Europe. Comparing OS in Spain 1991-1995 and 2001-2005 with results for Europe of the Automated Childhood Cancer Information System (ACCIS) (1988-1997) and the European cancer registry-based study on survival and care of cancer patients (EUROCARE) (2000-2007), it was similar for HL and lower for NHL and BL. CONCLUSIONS: Systematic monitoring and analysis of lymphoma paediatric data would provide clinical and epidemiological information to improve the health care of these patients and the outcomes for these malignancies in Spain.

Control integral de los factores de riesgo cardiovascular en diabetes tipo 2 en dos comarcas rurales / Integral control of cardiovascular risk factors in type 2 diabetes in the Pallars Jussà and Sobirà regions (area of influence of the regional hospital of Pallars-Lleida)

Fundamento y objetivos. El control de los factores de riesgo cardiovascular es especialmente importante en diabetes tipo 2 (DM-2). El grado de control no se ha estudiado específicamente en población rural ni cómo afecta a la calidad de vida. Hemos valorado el grado de consecución de los principales objetivos de control en el área adscrita a un hospital comarcal y evaluado la calidad de vida. Material y métodos. Estudio transversal, en pacientes DM-2 atendidos en atención primaria de las comarcas Pallars Jussà y Sobirà (Lleida). Se evaluaron el tabaquismo, IMC, PA, HbA1c, colesterol total, c-LDL, c-HDL y triglicéridos. La calidad de vida se valoró con cuestionario de salud (EQ-5D). Resultados. Se incluyeron 109 sujetos (55% hombres) con edad media (desviación estándar) de 70 (7,7) años. El 25% de los casos tenía IMC <27kg/m2 y 92% no fumaba. La PA sistólica y diastólica se hallaron controladas en el 36% y 53%, y la HbA1c, en 41%. Se halló control óptimo del colesterol total en el 54%, c-LDL en el 30%, c-HDL en el 64% y triglicéridos en el 75%. Un 1,8% alcanzaban todos los objetivos. La media de la valoración subjetiva global del estado de salud fue 69 puntos. Conclusiones. Una proporción muy pequeña de los pacientes con DM-2 se encuentran en los objetivos recomendados. Los más difíciles de conseguir fueron IMC y PA y los de mayor consecución fueron el nivel de triglicéridos y abstinencia tabáquica. La percepción subjetiva del estado de salud no se asoció al grado de control de objetivos(AU)

Fundamentals and objectives. Control of cardiovascular risk factors is especially important in type 2 diabetes (DM2). The degree of control has not been studied specifically in rural population and how it affects quality of life. We have assessed the degree of accomplishment of the main control objectives in the area assigned to a regional hospital and evaluated their quality of life. Material and methods. Cross-sectional study, in DM2 patients seen in Primary Care Centers on the Pallars Jussà y Sobirà (Lleida) regions. Smoking, body mass index (BMI), blood pressure (BP), HbA1c, total cholesterol, LDLc, HDL-c and triglycerides were evaluated. Quality of life was assessed with a health questionnaire (EQ-5D). Results. 109 subjects (55% male) were recruited, with a medium age (standard deviation) of 70 (7.7) years old. 25% of cases had BMI <27kg/m2 and 92% were non-current smokers. Systolic and diastolic BP were under control on the 36% and 53%, and HbA1c, on the 41%. Total Cholesterol was found optimal on the 54%, LDL-c on the 30%, HDL-c on the 64% and triglycerides on the 75%. 1.8% of patients achieved all the goals. The medium global subjective assessment of health status was 69 points. Conclusions. A very small percentage of patients with DM2 are in the recommended target. The most difficult to achieve were BMI and BP and the highest achievement were triglycerides and smoking abstinence. Subjective perception of health status was not associated with degree of objectives’ control(AU)

[Integral control of cardiovascular risk factors in type 2 diabetes in the Pallars Jussà and Sobirà regions (area of influence of the regional hospital of Pallars-Lleida)]. / Control integral de los factores de riesgo cardiovascular en diabetes tipo 2 en dos comarcas rurales.

UNLABELLED: FUNDAMENTALS AND OBJECTIVES: Control of cardiovascular risk factors is especially important in type 2 diabetes (DM2). The degree of control has not been studied specifically in rural population and how it affects quality of life. We have assessed the degree of accomplishment of the main control objectives in the area assigned to a regional hospital and evaluated their quality of life. MATERIAL AND METHODS: Cross-sectional study, in DM2 patients seen in Primary Care Centers on the Pallars Jussà y Sobirà (Lleida) regions. Smoking, body mass index (BMI), blood pressure (BP), HbA1c, total cholesterol, LDLc, HDL-c and triglycerides were evaluated. Quality of life was assessed with a health questionnaire (EQ-5D). RESULTS: 109 subjects (55% male) were recruited, with a medium age (standard deviation) of 70 (7.7) years old. 25% of cases had BMI <27kg/m(2) and 92% were non-current smokers. Systolic and diastolic BP were under control on the 36% and 53%, and HbA1c, on the 41%. Total Cholesterol was found optimal on the 54%, LDL-c on the 30%, HDL-c on the 64% and triglycerides on the 75%. 1.8% of patients achieved all the goals. The medium global subjective assessment of health status was 69 points. CONCLUSIONS: A very small percentage of patients with DM2 are in the recommended target. The most difficult to achieve were BMI and BP and the highest achievement were triglycerides and smoking abstinence. Subjective perception of health status was not associated with degree of objectives' control.

Fat accumulation in the rat during early pregnancy is modulated by enhanced insulin responsiveness.

Insulin sensitivity has been implicated in the variation of fat accumulation in early gestation by as-yet-unknown mechanisms. In the present study, we analyzed the insulin sensitivity of lipolysis and lipogenesis in lumbar adipocytes from rats at 0, 7, 14, and 20 days of gestation. In adipocytes of 7-day pregnant rats, we found a twofold decrease in both beta-agonist (isoproterenol and BRL-37344)-stimulated lipolysis and beta3-adrenoceptor protein but not in lipolysis initiated by forskolin or isobutylmethylxanthine, suggesting a modification of the lipolytic pathway at the receptor level. Whereas adipocytes from 7-day pregnant rats showed a twofold increase in fatty acid synthesis from glucose, those from 20-day pregnant animals displayed a decreased lipogenic activity. Insulin responsiveness of the lipolytic and lipogenic pathways was analyzed by dose-response experiments, giving evidence for the involvement of improved insulin responsiveness in the enhanced lipogenic and reduced lipolytic activities of adipocytes in early pregnancy. In contrast, insulin resistance is responsible for lower antilipolytic and lipogenic actions of insulin in late pregnant animals. In conclusion, the present study shows that enhanced adipose tissue insulin responsiveness during early pregnancy contributes to maternal fat accumulation, whereas decreased insulin responsiveness during late gestation modulates fat breakdown.

Evaluation of a portable respiratory recording device for detecting apnoeas and hypopnoeas in subjects from a general population.

This study was designed to validate a new home portable respiratory recording device (PRRD) to identify sleep apnoea and hypopnoea in a group of subjects (n=116), from a sample of the general population. Full night polysomnography (PSG) was used as the gold standard and simultaneously performed with PRRD. PRRD measurements included oronasal airflow (thermistry), chest wall impedance, oxygen saturation, snoring and body position. The sensors were unique for each recording system. Data obtained was blindly reviewed and analysed. A high level of agreement between both methods apnoea/hypopnoea index by PSG and the respiratory disturbance index (RDI) by PRRD was observed. Accuracy of the PRRD was evaluated in terms of sensitivity and specificity for different RDI-PRRD cut-off points with respect to AHI-PSG >10 and AHI-PSG >30. A logistic regression model was performed to estimate the chance per unit of RDI of apnoeas. A received operating characteristic (ROC) curve was drawn to obtain the sensitivity/specificity profile for each observed RDI value obtained. From the ROC curve the authors identified the better cut-off points, which represent a balanced sensitivity/specificity. Through a classification table defined by the cut-off point, the post-odds to exhibit the disease was calculated. For a full PSG cut-off point of 10 a PRRD of six showed a balanced sensitivity of 95% and a specificity of 92%. For a full PSG cut-off point of 30 a PRRD of 16 shows a balanced sensitivity/specificity (100% and 97%, respectively). Post odds of apnoea were calculated for each cut-off point. In conclusion, these data suggest that the portable respiratory recording device is an effective device to identify apnoeas and hypopnoeas in a general population and is therefore a suitable device to be used in epidemiological studies.

Effects of long-term amiodarone treatment on thyroid function.

Serum determinations of total T4, total T3, reverse T3, free T3 index, free T4, free T4 index, TBG, T4/TBG ratio, TSH and cholesterol were carried out on 18 euthyroid patients with coronary heart disease. Serum samples were obtained before treatment and after 15 days, 2, 4, 6, 8, 10, 12, 14 and 16 months of treatment with amiodarone (400 mg/day). Patients were divided into two groups, according to patterns of TSH response to thyrotrophin-releasing hormone (TRH): (I) patients with normal responses (n = 12), and (II) patients with subnormal responses (n = 6). Patients of group I showed total T4, free T4 and reverse T3 increments and total T3 and free T3 index decreases, whereas patients of group II were distinguished by the absence of T3 decreases and a rise in free T4 levels that showed a significant correlation with a drop in serum cholesterol (r = -0.767; p. less than 0.001). No patient of either group showed clinical signs of thyroid dysfunction. These results show that the appearance of hormonal patterns of hyperthyroidism is unpredictable and very frequent in patients with no previous thyroid abnormalities undergoing long-term treatment with the drug.