ABSTRACT
AIM: To determine the computer-predicted anticancer activity of antibiotic batumin. MATERIALS & METHODS: Cytotoxicity assays, cell morphology microscopy and cell cycle progression were studied in cancer and nontumorigenic cell lines. An in vivo experiment on Lewis lung carcinoma (3LL)-transplanted mice was conducted to evaluate potential antimetastatic. RESULTS & CONCLUSION: Cytotoxicity against melanoma and lung carcinoma cells (IC50 ≈ 5 µg/ml) was detected. Hypercondensed chromatin and apoptotic body formation in batumin-treated cells suggested the induction of apoptosis supported also by an observed increase in the quantity of cells occupying the sub-G1 cell cycle phase. Twofold reduction in the number and volume of lung metastases in Lewis lung carcinoma (3LL)-bearing batumin-treated mice was demonstrated. Highly specific cytotoxicity of batumin against cancer cell lines potentiates further studies.
Subject(s)
Anti-Bacterial Agents/pharmacology , Apoptosis/drug effects , Polyenes/pharmacology , Pseudomonas/chemistry , Animals , Anti-Bacterial Agents/chemistry , Anti-Bacterial Agents/therapeutic use , Binding Sites , Cell Line, Tumor , Female , G1 Phase Cell Cycle Checkpoints/drug effects , Humans , Lung Neoplasms/drug therapy , Lung Neoplasms/pathology , Mice , Mice, Inbred C57BL , Molecular Docking Simulation , Organic Chemicals/chemistry , Organic Chemicals/pharmacology , Organic Chemicals/therapeutic use , Polyenes/chemistry , Proto-Oncogene Proteins c-mdm2/chemistry , Proto-Oncogene Proteins c-mdm2/metabolism , Pseudomonas/metabolism , Transplantation, HeterologousABSTRACT
UNLABELLED: The present work was AIMED on the study of human beta-defensin 3 (hBD-3) gene expression in A431 cell line and human vulval tumors. MATERIALS AND METHODS: Twenty surgical specimens of both malignant and conventionally normal human vulval tissues have been analyzed for the presence of hBD-3 mRNA by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR). In vitro experiments have been carried out on A431 cells. RESULTS: It has been shown that hBD-3 gene expression in A431 cells was induced upon EGF stimulation. In human vulval tissues, in 8 cases the expression of hBD-3 gene was registered only in tumor tissue and was absent in paired controls, and 7 cases were characterized by significant increase of hBD-3 gene expression in malignant transformed epithelium in comparison with normal ones. Remaining surgical specimens showed either equal levels of hBD-3 expression or even moderate elevation of hBD-3 expression in normal tissue. In 2 specimens of tumor tissue hBD-3 mRNA was not observed. CONCLUSION: In human vulval epithelium, the increase in hBD-3 mRNA expression was predominantly associated with malignant phenotype.
