ABSTRACT
Wireless powered optogenetic cell-based implant provides a strategy to deliver subcutaneously therapeutic proteins. Immortalize Human Mesenchymal Stem Cells (hMSC-TERT) expressing the bacteriophytochrome diguanylate cyclase (DGCL) were validated for optogenetic controlled interferon-ß delivery (Optoferon cells) in a bioelectronic cell-based implant. Optoferon cells transcriptomic profiling was used to elaborate an in-silico model of the recombinant interferon-ß production. Wireless optoelectronic device integration was developed using additive manufacturing and injection molding. Implant cell-based optoelectronic interface manufacturing was established to integrate industrial flexible compact low-resistance screen-printed Near Field Communication (NFC) coil antenna. Optogenetic cell-based implant biocompatibility, and device performances were evaluated in the Experimental Autoimmune Encephalomyelitis (EAE) mouse model of multiple sclerosis.
Subject(s)
Encephalomyelitis, Autoimmune, Experimental , Multiple Sclerosis , Mice , Animals , Humans , Multiple Sclerosis/therapy , Encephalomyelitis, Autoimmune, Experimental/therapy , Interferon-beta/genetics , Interferon-beta/metabolism , Disease Models, Animal , Gene Expression , Mice, Inbred C57BLABSTRACT
Large-area paper batteries have been explored in this paper, correlating electrode materials and screen printing with the electrochemical performances. The use of office paper embedded in salt solution with two electrodes performed by an easy and large-scale application technique opens doors to a new concept of energy storage. The proposed device is Li-free and uses zinc and silver powder-based screen printable pastes to deposit the electrodes. Cyclic voltammetry and charge-discharge curves reveal the performance of the produced devices using NaCl and KOH solutions in different concentrations. The simulation of electrochemical impedance spectroscopy measurements gave clue of a similar working mechanism to conventional Li-ion batteries. After charging, a single paper battery achieves 1.83 V with 60 mA/cm2 and 90.6% charge-discharge efficiency. As a proof of concept, a small paper battery and a set integrated in series and parallel were used to power a commercial red light-emitting diode.
