ABSTRACT
Here we present an easy flow cytometry protocol to study the viability of Helicobacter pylori which also enables the detection of even low live bacteria densities. This protocol has potential utility for a fast and accurate assessment of experimental eradication methods against H. pylori.
Subject(s)
Bacteriological Techniques/methods , Flow Cytometry/methods , Helicobacter Infections/diagnosis , Helicobacter pylori/isolation & purification , Anti-Bacterial Agents , Helicobacter Infections/microbiology , Helicobacter pylori/cytology , Humans , Microbial Viability , Organic Chemicals , PropidiumABSTRACT
No disponible
Subject(s)
Humans , Male , Aged , Peritoneal Dialysis/adverse effects , Sphingomonadaceae , Anti-Bacterial Agents/pharmacology , Diabetic Nephropathies/complications , Diabetic Nephropathies/therapyABSTRACT
BACKGROUND: The combination of endobronchial biopsy with cytological brushing is considered the best procedures for the diagnosis of lung cancer during bronchoscopy. AIM: To asses the diagnostic yield for lung cancer (with visible endobronchial alterations at endoscopy) of three fiberoptic bronchoscopy procedures: endobronchial biopsy, cytological brushing and bronchial cytological washings. MATERIAL AND METHODS: Prospective study of 160 consecutive patients referred for fiberoptic bronchoscopy for clinically suspected lung cancer. Sequential bronchial washing, brushing and biopsies, were performed to all patients with a visible endobronchial alteration. RESULTS: One hundred and nine patients had a visible endobronchial alteration at fiberoptic bronchoscopy, but only 86 of them completed the protocol (21 patients were not subjected to the three diagnostic bronchoscopy procedures, because of bleeding or tolerance problems, and in two patients all the data required was not available). In twenty three, the diagnosis was a benign lesion, whereas lung cancer was diagnosed in 63. Fifty four of these 63 patients had at least one of the three endoscopic procedures (endobronchial biopsy, cytological brushing and bronchial cytological washings) positive for lung cancer (85.7%). The association of endobronchial biopsy with cytological brushing was positive for lung cancer in 49 cases (78%), and was the best association of two procedures. The diagnostic yield of each procedure was: endobronchial biopsy 50/63 (79%), cytological brushing 18/63 (28.5%). CONCLUSIONS: The best yield for the diagnosis of lung cancer is obtained with the association of the two procedures (endobronchial biopsy, cytological brushing). Cytological washings have a very low diagnostic yield.
Subject(s)
Bronchoscopy/methods , Lung Neoplasms/pathology , Lung/pathology , Biopsy/methods , Bronchoalveolar Lavage , Humans , Prospective StudiesABSTRACT
BACKGROUND: The rising antimicrobial resistance of streptococcus pneumoniae, requires permanent surveillance. Antimicrobial treatments of invasive infections must be modified accordingly. AIM: To assess the in vitro antimicrobial susceptibility of streptococcus pneumoniae strains isolated from lower respiratory infections. MATERIAL AND METHODS: Seventy five strains isolated during three periods in consecutive years were studied. In vitro susceptibility towards penicillin, erythromycin and ceftriaxone was studied using E-test technique minimal inhibitory concentrations. RESULTS: Five percent of strains were penicillin resistant and 11% had an intermediate sensitivity. Erythromycin resistance was observed in 1.3% of strains. Eight percent of strains had an intermediate sensitivity towards ceftriaxone and no resistance was observed. Comparing susceptibility in the three studied periods, it remained low and stable. CONCLUSIONS: High and intermediate resistance of Streptococcus pneumoniae towards penicillin is less prevalent than in other countries. A close surveillance must be maintained (Rev Méd Chile 2002; 130: 304-8).
Subject(s)
Anti-Bacterial Agents/pharmacology , Streptococcus pneumoniae/drug effects , Adult , Ceftriaxone/pharmacology , Cephalosporins/pharmacology , Drug Resistance, Bacterial , Erythromycin/pharmacology , Humans , Penicillins/pharmacology , Respiratory Tract Infections/microbiologyABSTRACT
We report a case of a case of a male patient with repeated hemoptysis without previous significant history and no weight loss, fever or sputum. Physical examination was normal and chest x-ray showed a mass in left upper lobe that was confirmed by CT scan. The mass touched the mediastinal fat and the mediastinal pleura anteriorly. Bronchocopy showed occlusion of left anterior segmental bronchus of the upper lobe with mucosal inflammation and hair was observed inside the bronchus. Thoracotomy was performed and a cystyc mass was fund in the left upper lobe, with no invasion of the mediastinum, and was totally resected. Pathology specimen was compatible with the diagnosis of a cystic teratoma with several different tissues which included pancreatic parenchyma. The patient's outcome was uneventful and he continues to do well during follow-up
Subject(s)
Humans , Female , Adult , Teratoma , Lung NeoplasmsABSTRACT
Se revisan los aspectos clínicos de 18 infecciones pleuropulmonares por Bacteroides melaninogenicus. La mayoría de los pacientes presentaba condiciones predisponentes, tales como alcoholismo (4), cirugía torácica y cervical reciente (3), cáncer pulmonar (2), diabetes (1), epilepsia (1), hemotórax (1). Sólo dos pacientes no presentaban factores de riesgo. Los hallazgos radiológicos consistieron en condensación pulmonar en 4, neumonia necrotizante en 3, absceso pulmonar en 3 y derrame pleural en 7. La mayoría de los pacientes tuvo una respuesta deficiente al tratamiento con penicilina, lo cual sugiere resistencia bacteriana o bien inactivación del antibiótico por bacterias aeróbicas presentes en los cultivos. Los pacientes con compromiso pleural curaron con drenaje quirúrgico. Sugerimos que los casos en que se sospecha neumonia anaeróbica con lenta resolución de la infección deberían estudiarse con procedimientos invasivos a fin de identificar al agente causal
