ABSTRACT
As part of the European BIOTECH programme, the nucleotide sequence of a 16691 bp fragment from the left arm of chromosome IV of Saccharomyces cerevisiae has been deduced. Analysis of the sequence reveals the presence of 13 open reading frames (ORFs) larger than 100 codons. five of these were previously identified as genes DUN1, PMT1, PMT5, SRP14 and DPR1. One putative protein, D2371p, contains an ATP-GTP binding site, and shares homology to the ArsA component of an Escherichia coli arsenical pump. No significant homology to any known protein has been found for the other ORFs. D2378p contains a zinc finger domain.
Subject(s)
Cell Cycle Proteins , Fungal Proteins/genetics , Mannosyltransferases/genetics , Open Reading Frames/genetics , Protein Kinases/genetics , Saccharomyces cerevisiae Proteins , Saccharomyces cerevisiae/genetics , Signal Recognition Particle/genetics , Transferases/genetics , Amino Acid Sequence , Cloning, Molecular , Electronic Data Processing , Molecular Sequence Data , Plasmids , Protein Serine-Threonine Kinases , Restriction Mapping , Sequence Alignment , Sequence Homology, Amino AcidABSTRACT
We report the DNA sequence of a 17,933 bp fragment from the left arm of chromosome XIV of Saccharomyces cerevisiae. Analysis of the sequence reveals the presence of ten open reading frames (ORFs) larger than 100 codons. Four of these were previously identified as genes RHO2, TOP2, MKT1 and END3. Additionally, the NH2 end coding region of PMS1 is found in the 3' end of the sequence. No significant homology to any known protein has been found for the other five ORFs.
Subject(s)
Chromosomes, Fungal/genetics , Genes, Fungal/genetics , Open Reading Frames/genetics , Saccharomyces cerevisiae/genetics , Amino Acid Sequence , Fungal Proteins/genetics , Molecular Sequence Data , Sequence Analysis, DNA , Sequence Homology, Amino AcidABSTRACT
The nucleotide sequence of a fragment of 21 308 bp from the left arm of Saccharomyces cerevisiae chromosome XIV has been determined. Analysis of the sequence revealed 13 open reading frames (ORFs) longer than 300 bp, four of which correspond to the previously identified genes LEU4, MET4, POL1 and RAS2. One putative protein, N2160, shares considerable homology (32% identity) with a hypothetical protein encoded by a gene located on chromosome XV as well as with human OCRL protein (36% identity), involved in Lowe's syndrome. N2185 contains ten predicted transmembrane segments and is similar to another putative protein (YKL146) from yeast.
