Subject(s)
Amylases/blood , Gastric Juice/chemistry , Nephrotic Syndrome/drug therapy , Prednisone/adverse effects , Amylases/urine , Bicarbonates/chemistry , Blood Glucose/drug effects , Child , Creatinine/blood , Creatinine/urine , Gastric Acidity Determination , Humans , Insulin/blood , Nephrotic Syndrome/blood , Nephrotic Syndrome/urine , Pancreatic Extracts/chemistry , Prednisone/administration & dosageABSTRACT
Twenty cases of thalassemia major and 20 children with glucose-6-phosphate dehydrogenase deficiency were studied for the protein content of their red cell membrane as well as for its fractions, compared to 20 normal controls. The results showed that the total membrane proteins per red cell and the various fraction percentages in both conditions were not significantly different from normal controls. Also the acrylamide gel electrophoretic studies of the red cell membrane proteins gave band patterns identical with those of normal controls. These results are discussed.
Subject(s)
Erythrocyte Membrane/analysis , Erythrocytes/analysis , Glucosephosphate Dehydrogenase Deficiency/blood , Membrane Proteins/analysis , Thalassemia/blood , Anemia, Hemolytic/blood , Child , Child, Preschool , Female , Glucosephosphate Dehydrogenase Deficiency/physiopathology , Humans , Infant , Liver/physiopathology , Liver Function Tests , Male , Thalassemia/physiopathologyABSTRACT
7 infants diseased with Acrodermatitis enteropathica and 10 normal controls were included in this study. The values of anthranilic acid glucuronide, 6- aminohippuric, anthranilic acid, N-acetyl Kneurine, Kneurine and 30 H Kneurenine, were estimated in mg/24 hours urine, both basal and after tryptophane load. In addition, histopathological and histochemical studies for lactase, succinic dehydrogenase, alkaline phosphatase, acid phosphatase, and alpha-non-specific esterases activities were done for the intestinal mucosal biopsies. All the previous investigations were then repeated after two months treatment with 500 mg/day diiodohydroxyquinoline. The tryptophan metabolites were significantly low in the diseased infants, both basal and after tryptophan load. Moreover, the intestinal enzymes activities were altered. After 2 months treatment with diiodohydroxyquinoline the diseased infants became clinically improved, tryphtophan metabolites became normal, but the activities of the intestinal enzymes were not altered. The biochemical and histochemical findings were discussed, giving the possibility of competitive inhibition of the diiodohydroxyquinolines and the by-product 8 OH Quinololic acid resulting in more degradation of Kneurine and 3 OH Kneurenine to nicotinamide adenine dinucleotide.
Subject(s)
Acrodermatitis/metabolism , Intestinal Diseases/metabolism , Intestinal Mucosa/enzymology , Tryptophan/metabolism , Acrodermatitis/drug therapy , Esterases/metabolism , Histocytochemistry , Humans , Infant , Intestinal Diseases/drug therapy , Iodoquinol/therapeutic use , Succinate Dehydrogenase/metabolism , beta-Galactosidase/metabolismABSTRACT
Histopathological and histochemical studies were done on renal biopsies taken from twenty nephrotic children and thirty Albino rats of which fifteen were rendered nephrotic by the intravenous injection of nephrotoxic serum and the other fifteen served as controls. There were reduced activities of succinic dehydrogenase, alkaline phosphatase, acid phosphatase and non-specific esterases enzymes. These changes may be secondary to the insult which caused the nephrotic syndrome, or the result of the heavy proteinuria. In conclusion, a derangement in carbohydrate metabolism may underlie the disturbed metabolism of the basement membrane, the distortion of the foot-processes, and the tubular dysfunction.
