Search | VHL Regional Portal

Systematic analysis of oxidative degradation of polysaccharides using PAGE and HPLC--MS.

Ovalle, R; Soll, C E; Lim, F; Flanagan, C; Rotunda, T; Lipke, P N.

Carbohydr Res ; 330(1): 131-9, 2001 Jan 15.

Article in English | MEDLINE | ID: mdl-11217956

ABSTRACT

Oxidation of polysaccharides yields hydroxyaldehydes and hydroxycarboxylic acids. Aldehydes and carboxylic acids were separately conjugated to 8-aminonaphthalene-1,3,6-trisulfonic acid (ANTS) or tyrosine t-butyl ester (TBT). The ANTS-labeled derivatives were separated by molecular size on PAGE gels and detected by fluorescence. TBT-labeled derivatives were separated by reverse phase chromatography on a C18-HPLC column and analyzed by positive ion electrospray mass spectroscopy (HPLC--MS). This combination of procedures allowed a systematic analysis of carbohydrate oxidation products.

Subject(s)

Polysaccharides/metabolism , Spectrometry, Mass, Electrospray Ionization/methods , Aldehydes/chemistry , Aldehydes/metabolism , Carboxylic Acids/chemistry , Carboxylic Acids/metabolism , Chromatography, High Pressure Liquid , Electrophoresis, Polyacrylamide Gel , Hydrogen Peroxide/metabolism , Iron/metabolism , Oxidation-Reduction , Superoxides

Combinatorial synthesis and modification of functional porphyrin libraries: identification of new, amphipathic motifs for biomolecule binding.

Drain, C M; Gong, X; Ruta, V; Soll, C E; Chicoineau, P F.

J Comb Chem ; 1(4): 286-90, 1999.

Article in English | MEDLINE | ID: mdl-10748737

Subject(s)

Combinatorial Chemistry Techniques/methods , Porphyrins/chemistry , Porphyrins/chemical synthesis , Drug Design , Molecular Structure

ABSTRACT

Subject(s)

Subject(s)

SEND TO:

SELECTION OF CITATIONS

SEARCH DETAIL