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1.
Lab Delo ; (3): 52-5, 1990.
Article in Russian | MEDLINE | ID: mdl-1692911

ABSTRACT

Chemiluminescent characteristics of the antigen-antibody reaction, effects of gamma-globulin, iodine, hydrogen peroxide, and temperature on the type of the fluorescence of the complex were examined with the use of biomedical chemiluminometer and fluorescent spectrophotometer (Japan). The findings evidence that fluorescence is connected mainly with the tryptophan amino acid residues and with conformational restructuring of protein molecules. Changes in the intensity of chemiluminescence during the formation of a complex may be used for the detection of antibodies in the examinee's body.


Subject(s)
Antigen-Antibody Reactions , Luminescent Measurements , Animals , In Vitro Techniques , Rabbits
2.
Article in Russian | MEDLINE | ID: mdl-2816191

ABSTRACT

The article discusses a new method for recognising tumors of the spinal cord--spontaneous and photoinduced chemoluminescence of the cerebrospinal fluid. Twenty-seven patients with tumors of the spinal cord of various localization and histological structure and 15 individuals of the control group were examined. Statistically significant differences in chemoluminescence in extra- and intramedullary tumors and in the control group were revealed. The effect of intensified luminescence of the cerebrospinal fluid in exposure to ultraviolet radiation was demonstrated, especially in cases with extramedullary tumors. The described method is an auxiliary criterion in c.s.f. diagnosis of spinal tumors.


Subject(s)
Luminescent Measurements , Spinal Cord Neoplasms/cerebrospinal fluid , Biophysical Phenomena , Biophysics , Cerebrospinal Fluid/radiation effects , Humans , Lumbar Vertebrae , Mathematics , Osteochondritis/cerebrospinal fluid , Spinal Cord Neoplasms/diagnosis , Ultraviolet Rays
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