ABSTRACT
The lifetime research of the oxidized and methilated guanine's contents in urine of intact and radiated mice was carried out. For statistical processing of results we used the method of the trial periods. This method has been created by A.A. Konradov, but has not been published during a life of the author. It has allowed finding out oscillatory part of age-related dependences. For intact animals the most probable (p = 0.001) is the period of 7 months. The infringement of a seasonal rhythm of fluctuations of oxidized and methilated guanine's concentration in urine is marked for the radiated mice. It is specific feature of the radiation-accelerated ageing.
Subject(s)
Guanine/urine , Head/radiation effects , Models, Biological , Seasons , Animals , Biomarkers/urine , Female , Guanine/metabolism , Longevity/radiation effects , Methylation , Mice , Mice, Inbred CBA , Oxidation-Reduction , X-RaysABSTRACT
The research is devoted to the analysis of individual dependencies of weight of a body, rectal temperature and functional motor asymmetry of behavior of mice female CBA from age received in longitudinal research. Both individual, and the average age dependencies of the above named parameters have an extreme (nonlinear) kind. It is revealed, that by the age of achievement of the maximal weight (Tm) and greatest asymmetry of behavior (Tn) are connected with each other (r = 0.6, p < 0.001). The difference (Tm-Tn) is linearly connected to life expectancy (r = 0.62, p < 0.001), that is this parameter can be used for the forecast of individual life expectancy.
Subject(s)
Aging/physiology , Animals , Behavior, Animal , Body Temperature , Body Weight , Female , Mice , Mice, Inbred CBA , RectumABSTRACT
Difference between left and right cerebral hemisphere potential (mV) is biophysical reflection of brain asymmetry. Cymbate changes of constant potential run during the life in both hemisphere of brain of mice. Number turning on the right of 10 attempt in T maze is a quantitative criteria of functional motor asymmetry. Manifest right asymmetry of behavior of mice observe only from 12 to 17 month old. Ambylateral behavior is character to animals younger and older this period. Thus, as difference between left and right cerebral hemisphere potential both motor asymmetry have nonlinear change with age. Coefficient of correlation between this parameters equal -0.42 for young (3-7 month) mice, +0.53 for old (21-28 month) mice. But this coefficient is near zero for mice from 11 to 19 month old. Results of our research show, that quality change in brain run in middle age.
Subject(s)
Aging/physiology , Brain/physiology , Functional Laterality/physiology , Motor Skills/physiology , Age Factors , Animals , MiceABSTRACT
Aging of female CBA mice was accelerated by irradiation of the head at 10 and 12 Gy. The mean life span was shortened by 10.5 and 21%, respectively. The distribution by life span was similar in the control and experimental groups. The level of DNA oxidation products in the urine was normalized within 28 days after irradiation. At the age of more than 7.5 months, the irradiated mice had a lower body weight. Age-related changes of body temperature in irradiated (10 Gy) and control mice mostly coincided; the differences were evident after 18 months. It was concluded that physiological and radiation-induced aging are significantly similar.
Subject(s)
Aging/physiology , Aging/radiation effects , Animals , Body Temperature/physiology , Body Temperature/radiation effects , Body Weight/physiology , Body Weight/radiation effects , DNA/radiation effects , Female , Guanine/analogs & derivatives , Guanine/urine , Head/radiation effects , Longevity/physiology , Longevity/radiation effects , Mice , Mice, Inbred CBA , Oxidation-ReductionABSTRACT
We studied the changes in functional motor asymmetry in female CBA mice during the course of natural aging and after irradiation of the head with 10 Gy. The functional motor asymmetry was assessed by the behavior of the animal in a T-shaped maze. The proportion of right turns in ten attempts was recorded for each mouse and the asymmetry index was calculated for the population. The asymmetry-age relationship in irradiated and intact mice has a similar shape with a distinct peak at the age of 13-17 months. The control population features a predominance of right turns, while left turns are specific for the irradiated one. This behavioral inversion is due to radiation-induced brain disturbance: the dominant left hemisphere proved to be more sensitive. Possible different rates of hemisphere aging is discussed.
