[The role of heterologous helper cells in the production of gamma interferon by T-lymphocytes of human blood and lymph]. / Rol' geterologichnykh vspomogatel'nykh kletok v produktsii gamma-interferona T-limfotsitami krovi i limfy.

The results of studies of the accessory functions of various heterologous cells in gamma interferon production by human blood and lymph T-lymphocytes are presented. It was shown that, in addition to autologous and allogeneic monocytes, the role of accessory cells may be played to some extent by xenogeneic (mouse) monocytes as well as human continuous cells of different origin. The degree of the accessory function was different in interaction of the accessory cells with T-lymphocytes from blood and lymph.

[Producer cells of human alpha and gamma interferons]. / Kletki--produtsenty al'fa- i gamma-interferonov cheloveka.

The cells responsible for production of human alpha and gamma interferons with proper inducers were studied using methods for fractionation of human blood mononuclear cells. The results indicated cooperation and mutual regulation by cells of interferon production. Monocytes were shown to play the leading role in the synthesis of acid-stable alpha interferon, whereas accessory cells were required for gamma interferon synthesis by T lymphocytes. Acid-labile alpha interferon was produced predominantly by B cells with accessory participation of T lymphocytes.

[Preparation and properties of the staphylococcal toxin causing the toxic shock syndrome]. / Poluchenie i nekotorye svoistva stafilokokkovogo toksina, vyzyvaiushchego sindrom toksicheskogo shoka.

A method is proposed for isolation and purification of the staphylococcal toxin causing the toxic shock syndrome (TSS). The method includes three steps: aggregation of protein from the cultural filtrate of Staphylococcus aureus strain 1169 in the presence of 0.025% sodium hexametaphosphate at pH 3.0; gel filtration of the concentrated material on the sephadex G75; ion-exchange chromatography on DEAE 32 cellulose. The proposed method permits to obtain the purified biologically active preparation of toxin with the yield about 40%. The obtained preparations are homogeneous in polyacrylamide electrophoresis and as analyzed by immunochemical methods. The mol mass of the isolated protein is 24 kD, it is not immunologically identical to staphylococcal toxins A-D and is lethal for New Zealand white rabbits and chinchilla rabbits. Interferon inducing activity of the protein is identical to the one of staphylococcal enterotoxin type A.

[Use of filter-adsorbed enterotoxins for isolating human immune interferon]. / Ispol'zovanie énterotoksinov, adsorbirovannykh na fil'trakh, dlia polucheniia immunnogo interferona cheloveka.

The use of A and B enterotoxins of St. aureus adsorbed on Millipore nitrocellulose filters for induction of donor blood leukocytes resulted in production of immune interferon free of the inducer. The interferon produced by this method is acid-labile and inactivated by antiserum to human immune interferon but not to human leukocyte interferon. The advantage of this type of induction consists in the possibility of multiple use of the filters with adsorbed enterotoxins.

[Non-specific antitumoral activity of staphylococcal enterotoxins]. / Protivoopukholevaia nespetsificheskaia aktivnost' stafilokokkovykh énterotoksinov.

St. aureus enterotoxins A and B possess an antitumour effect. After intraperitoneal inoculation they decrease the size and in some cases prevent the development of the human hypernephroma in the cheek pouch of golden hamsters. The effect of enterotoxins may possibly consist in inducing the production of endogenous immune interferon which activates the host immune system and enhances the rejection of heterologous tumour cells.

[Production of alpha- and gamma-interferons by human central lymph cells in different pathological states]. / Produktsiia al'fa- i gamma-interferonov kletkami tsentral'noi limfy cheloveka pri razlichnykh patologicheskikh sostoianiiakh.

The IF-producing capacity of human lymphocytes obtained by drainage of thoracic duct of surgical patients was studied. The lymph cells incubated with the appropriate inducer produced interferon of alpha and gamma types. The level of IF production, particularly of the gamma type, was negatively influenced by the storage of lymph at +4 degrees C and recovery of the cells in ficoll-hypaque gradient. The time course of IF production was found on the whole to be similar to that in the peripheral blood lymphocytes. The level of alpha-IF synthesis was higher for blood cells, and that of gamma-IF for lymph cells. The synthesizing capacity of the thoracic duct lymphocytes decreased in the acute stage of the disease and increased during convalescence which was especially typical of gamma-IF production.

[Production of gamma and alpha interferons by human blood leukocytes in sequential induction]. / Produktisiia gamma- i al'fa-interferona leikotsitami krovi cheloveka v usloviiakh posledovatel'noi induktsii.

A schedule for human gamma and alpha interferon production by successive administration, at 2-3-day intervals, of the proper inducers (phytohemagglutinin and Newcastle disease virus (NDV), or staphylococcal enterotoxin A and NDV) into the same suspensions of peripheral blood leukocytes has been proposed. The schedule may be used in laboratory practice and manufacture.