[Oxidation-reduction potential of tissues of the oral mucosal wound surface under the photodynamic action]. / Okislitel'no-vosstanovitel'nyi potentsial tkanei ranevoi poverkhnosti slizistoi obolochki polosti rta pri fotodinamicheskom vozdeistvii.

The purpose of the study was to examine the condition of the pro-antioxidant balance of the homogenates of tissues of the oral mucosal wound surface in vivo experiment under the photodynamic action of combined two-wave radiation to eliminate its damaging effect on the structures of cell membranes. MATERIALS AND METHODS: On the mucous membrane of the cheeks of sexually mature female autocrats of Wistar rats (n=72) experimental models of wound surfaces with a diameter of 3 mm were formed. The animals were divided into groups: control (n=36) and experimental (n=36). In the experimental group, a single photodynamic exposure was carried out for the 1st postoperative day with a sequential combined light radiation at two wavelengths (660 nm and 405 nm) with a total dose of 15 J/cm2. Wound management was carried out traditionally in the control group of animals. The observation periods were 3, 7 and 14 days. RESULTS: Macroscopic examination of the wound surface indicated complete epithelialization of the wound on the 7th day in the experimental group of animals and on the 14th day in the control group. Significant and sharp decline in free-radical oxidation processes (Imax, S, Z index) intensity was recorded in the experimental animals at earlier periods in comparison with the initial data and control results, when analyzing the results of studies of homogenates of animal tissues. A similar pattern was observed in the evaluation of lipid peroxidation products, especially in the early days of observations. The reported results were supported by the activation of antioxidant enzymes in the experimental group compared to control data and baseline data. CONCLUSION: Thus, a combined photodynamic action using two wavelengths with a combined dose of 15 J/cm2 provides safety and low invasiveness for use on the oral mucous membrane and, may be used to stimulate regeneration of the wound surface, which requires further experimental testing.

[The effect of subchronic inhalations of nitric oxide on metabolic processes in blood of experimental animals].

Metabolic processes were investigated in plasma and erythrocytes of Wistar rats exposed to daily 10-min sessions of NO inhalation for 30 days. These included determination of glucose and lactate, catalase activity, and activities of aldehyde dehydrogenase (ALDH), lactate dehydrogenase (LDH), and catalase. NO inhalation in a concentration of 20 ppm, 50 ppm and 100 ppm caused an increase in glucose and lactate. Inhalation of 100 ppm NO also increased catalase activity. Inhalation of all NO concentrations resulted in a decrease of ALDH activity, while the decrease in LDH activity was observed at NO concentrations of 50-100 ppm.

[Comparative analysis of action of nitric oxide as a free radical and its storage form on the state of pro- and antioxidant blood systems].

The dynamics of oxidative metabolism in healthy people's blood (n = 30) under the influence of gaseous nitric oxide and dinitrosyl iron complexes is explored. In all blood samples we studied lipid peroxidation intensity and malonic dialdehyde in plasma and erythrocytes, plasma antioxidant potential and activity of superoxide dismutase. During our investigations it was possible for the first time to identify the peculiarities in the responses of pro- and antioxidant blood systems in vitro to the treatment with nitrogen monoxide as the free. radical and its storage form (as a component of dinitrosyl iron complexes). So, a pronounced prooxidant effect for the gas flow from the "Plazon" apparatus moderately decreases when a tenfold dilution of a NO-containing mixture is made. Gas flow from the experimental NO-generator causes minimal prooxidant action, and injection of water solution of dinitrosyl iron complexes in blood specimens leads to an antioxidant action, as limitation of lipoperoxidation processes in plasma and stimulation of superoxide dismutase in erythrocytes.

[Influence of nitric oxide inhalations on oxidative and energy metabolism of blood in rats].

The aim of this work was the investigation of nitric oxide (NO) inhalations action on some parameters of energy and oxidative metabolism of healthy rats blood. Wistar rats were randomly divided into two groups: control group (without any manipulations; n = 10) and main group (n = 10) with inhalations by NO-containing gas flow (20 ppm). Activity of pro- and antioxidant systems, superoxide dismutase, lactate dehydrogenase and lactate level were estimated in rats blood samples. We observed stimulation of adaptive reserves of rats organism after course of NO (20 ppm) inhalations. First component of these changes was a modulation of pro- and antioxidant systems, including intensification of lipid peroxidation with prevalent activation of superoxide dismutase. Second component was a positive modification of lactate dehydrogenase functioning.