ABSTRACT
This study reports sequence characteristics and population genetic data on a 'new' STR locus HumHUU (D16S3433) located in the non-coding region of chromosome 16q. Based on a population sample of 306 non-related Polish individuals 205 genotypes and 15 alleles with length range of 157-211bp were distinguished. No deviation from HWE was observed. The sequence analysis of each D16S3433 allele revealed a tetranucleotide repeat motif with a basic sequence structure (AAAA)(0-1)(AAAG)(11-22)(AAAAG)(AAAA)(AG)(AAAAAAG). The power of discrimination is 0.9538, showing a high degree of polymorphism. The presented results demonstrate that the D16S3433 is a useful genetic marker for forensic purposes and paternity testing.
Subject(s)
Microsatellite Repeats/genetics , Alleles , Base Sequence , DNA Primers , Humans , Polymerase Chain Reaction , Quality ControlABSTRACT
PURPOSE: The objective of this project was to establish an 'in-house' DNA database to store and compare profiles genotyped in the Department of Forensic Medicine, Medical University of Bialystok. MATERIAL AND METHODS: DNA was extracted using Chelex-100, an organic procedure, or commercial kits. Genetic profiles were obtained using AmpFISTR SGM Plus, AmpFISTR Profiler, or AmpFISTR Identifiler and 310 ABI Prism Genetic Analyzer (Applera). DNA Stat v. 1. 2 software was used to construct the database. RESULTS: As of the end of 2006 our forensic database stored 1,595 profiles genotyped in criminal cases in the years 2000-2006, including 398 non-match samples, 2 non-match fetuses, 5 non-match newborns and 4 non-match corpses. CONCLUSION: A DNA database was established that may be used for the purpose of genetic profile comparison in criminal cases.
Subject(s)
Criminology , DNA Fingerprinting , Databases, Genetic , Forensic Medicine , Software , Humans , PolandABSTRACT
PURPOSE: Urine specimens are usually collected for biochemical and toxicological tests and for doping control. In forensic casework urine analyses are performed occasionally, however, the authors emphasize their importance in crime scene reconstruction. The objective of the research was to evaluate efficacy of AmpFISTR SGM Plus typing of urine and urine stains which were subject to different temperature conditions. MATERIAL AND METHODS: Urine samples were collected from 10 female and 10 male volunteers. Liquid specimens were stored at room temperature (RT), 4 degrees C and -20 degrees C up to 28 days. Experimental stains were prepared by applying 3 ml urine on sterile cloth 30 x 30 cm, air-dried and stored at RT up to 360 days. The amount of DNA was estimated with use of slot-blot technique (Quantiblot Human DNA Quantitation Kit, Applera). DNA profiles were obtained using AmpFISTR SGM Plus and 310 ABI Prism Genetic Analyzer (Applera). Typing of a experimental sample was considered successful when the full profile was obtained matching that of a reference sample. RESULTS: Significant differences in DNA yield were noted between female and male urine samples. No differences between the extraction methods were found in regard to DNA yield and typeability rate. Different typeability rates were recorded for liquid urine and urine stains depending on storage temperature. CONCLUSIONS: Liquid urine samples and urine stains can be considered as a potential source of DNA in disputable specimen individualization and in forensic casework using the fluorescent multiplex PCR system AmpFLSTR SGM Plus.
Subject(s)
DNA Fingerprinting/methods , DNA/urine , Female , Humans , Male , Polymerase Chain Reaction/methods , Reproducibility of ResultsABSTRACT
BACKGROUND: STR systems are widely used in forensic casework due to their discriminating power and high sensitivity. The aim of the study was to determine allele frequencies and forensic efficiency parameters of the STRs D8S1132, CD4, VWA and TH01 in a population sample of Podlasie (Northeastern Poland). MATERIAL AND METHODS: Blood specimens were collected from the unrelated individuals in Podlasie, Poland. The DNA samples were extracted with Chelex method and amplified by PCR technique. The PCR products were analyzed by the PAG horizontal electrophoresis with discontinuous buffer system and then silver stained. RESULTS: All common alleles included in the allelic ladders were found. For all loci examined no deviations from Hardy-Weinberg equilibrium were found. CONCLUSION: The TH01, VWA and D8S1132 systems have been confirmed as particularly useful in the paternity testing and the individualization of biological evidence.
Subject(s)
CD4 Antigens/genetics , DNA/blood , Genetic Markers , Genetics, Population , Tandem Repeat Sequences , Alleles , Humans , Male , Paternity , Poland , Polymerase Chain ReactionABSTRACT
Allele frequencies for 15 STR loci included in AmpFlSTR Profiler and AmpFlSTR SGM Plus kits were determined in a sample of 413 unrelated individuals living in the region of Podlasie (NE Poland)
