ABSTRACT
The genotoxic and antigenotoxic effects of Cotinus coggygria Scop. methanol extract was investigated using the Drosophila sex-linked recessive lethal (or SLRL) test. The results presented here show that the methanol extract of Cotinus coggygria in a concentration of 5% and artificial chemical agent ethyl methanesulfonate EMS (0.75 ppm) induce recessive lethal mutations on X-chromosome on Drosophila melanogaster in all broods (I, II and III). Post-treatment with lower concentration of the methanol extract of Cotinus coggygria (2%) was effective in reducing genotoxicity ofmutagen.
Subject(s)
Anacardiaceae/chemistry , DNA Damage/drug effects , Methanol/chemistry , Mutagens/pharmacology , Plant Extracts/pharmacology , Animals , Drosophila melanogaster , Mutagens/chemistry , Plant Extracts/chemistry , X Chromosome/genetics , X Chromosome/metabolismABSTRACT
The aim of this study was to examine six plants from Serbia for their potential antioxidant activity. Therefore, six antioxidant activity assays were carried out, including: total antioxidant capacity, DPPH free-radical scavenging, the inhibitory activity toward lipid peroxidation, Fe(3+)- reducing power, Fe(2+)- chelating ability and hydroxyl radical scavenging activity. Total phenolic and flavonoid contents were also determined for each alcoholic extract. Cotinus coggygria extract contained the highest amount of total phenols (413mg GAE /g dry extract), while the highest proportion of flavonoids was found in the Echium vulgare methanol extract (105 mg RU/g). Cotinus coggygria and Halacsya sendtneri alcoholic extracts showed the highest total antioxidant capacity (313 and 231 mg AA/g dry extract), as well as DPPH free-radical scavenging (IC(50)=9 and 99 µg/ml), inhibitory activity toward lipid peroxidation (IC(50)=3 and 17 µg/ml) and reducing power. Whereas, the greatest hydroxyl radical scavenging activity, as well as ferrous ion chelating ability showed Echium vulgare, Echium rubrum and Halacsya sendtneri.
Subject(s)
Free Radical Scavengers/pharmacology , Lipid Peroxidation/drug effects , Magnoliopsida/chemistry , Plant Extracts/pharmacology , Biphenyl Compounds/metabolism , Chelating Agents/pharmacology , Ferrous Compounds/metabolism , Flavonoids/analysis , Free Radical Scavengers/chemistry , Free Radicals/metabolism , Indicators and Reagents/metabolism , Phenols/analysis , Picrates/metabolism , Plant Extracts/chemistryABSTRACT
The results of a preliminary antifungal screening of the methanol extracts and the major anthraquinone aglycones, alizarin and emodin, of Rubia tinctorum and Rhamnus frangula in comparison with the antifungal activity of the anthraquinone-containing lichen Caloplaca cerina and its main secondary metabolite parietin are reported.
