Comparative Evaluation of Cotton PTFE Tape and Foam Pellets as Endodontic Spacer in Primary Teeth: An In Vivo Study.

Aim and objective: This study was conducted to microbiologically evaluate cotton, PTFE tape, and foam pellets as endodontic spacer in primary teeth. Materials and methods: Thirty primary second molars indicated for pulpectomy were included in this study. Followed by the completion of pulpectomy in each teeth cotton, PTFE tape, and foam pellets were placed as endodontic spacers randomly in 10 teeth each. The samples were collected from the pulp chamber at baseline and after 7 days to evaluate for microbial contamination of the same. The data obtained was tabulated and subjected to appropriate statistical analysis. Results: There was a statistically significant increase in the colony forming unit after 7 days in cotton and foam pellet group. But PTFE tape group showed very minimal contamination of the pulp chamber after 7 days. Conclusion: Within the limitations of this study, it can be concluded that PTFE tape found to be the best alternative to cotton as an endodontic spacer. Clinical significance: In case of multiple visit pulpectomy procedure, the state of sterility must be maintained in between the appointments until a definitive coronal restoration is placed following root-canal obturation. So it is mandatory to place an endodontic spacer under a provisional restoration to maintain the patency of the root canals and prevention of the microbial growth. The PTFE tape is inorganic, nonfibrous material which can be tightly packed without any voids under the provisional restoration. So PTFE tape is the best material to maintain the root canal patency and to provide a sterile environment by preventing the microbial growth under the provisional restoration as an endodontic spacer material in between the appointments. How to cite this article: Somani R, Arya MV, Singh DJ, et al. Comparative Evaluation of Cotton PTFE Tape and Foam Pellets as Endodontic Spacer in Primary Teeth: An In Vivo Study. Int J Clin Pediatr Dent 2022;15(1):74-78.

Comparative Evaluation of Microleakage in Various Placement Techniques of Composite Restoration: An In Vitro Study.

AIM: The study was conducted to evaluate and compare the effect of various placement techniques of composite restoration on microleakage. MATERIALS AND METHODS: Forty extracted premolars were selected and a rectangular-shaped cavity was prepared on the buccal surface of cervical third of each tooth. Thereafter, teeth were randomly divided into four groups equally and were restored with the composite restorative material with different placement techniques, i.e., bulk placement technique, horizontal incremental technique, split incremental technique, and newly introduced Mat incremental technique. Samples were thermocycled and immersed in methylene blue dye for 24 hours. The samples were then sectioned and evaluated under a stereomicroscope for microleakage. RESULTS: Microleakage was present least in the Mat incremental group and maximum in the bulk placement group while intercomparison revealed statistically significant difference between all the groups except for split incremental and Mat incremental groups. CONCLUSION: The recently introduced Mat incremental placement technique showed least microleakage when compared to conventional techniques. HOW TO CITE THIS ARTICLE: Somani R, Som NK, Jaidka S, et al. Comparative Evaluation of Microleakage in Various Placement Techniques of Composite Restoration: An In Vitro Study. Int J Clin Pediatr Dent 2020;13(3):264-268.

A comparative evaluation of compressive strength and antimicrobial efficacy of Fuji IX and Amalgomer CR: An in vitro study.

PURPOSE: To evaluate and compare the compressive strength and antimicrobial efficacy of Amalgomer CR and Fuji IX. METHOD: 40 restorative pellets were taken for evaluating compressive strength and 40 blood agar petridishes for antimicrobial efficacy. 40 restorative pellets were fabricated from the tested materials according to manufacturer intructions. Then the pellets were subjected to compressive strength testing using Instron Universal Testing machine. Antibacterial activity of Amalgomer CR and Fuji IX was assessed using the agar diffusion test. The powder and liquid of each test material was mixed and inserted in the punched wells (6 mm × 2 mm) in agar petridishes. An empty well was taken as control to authenticate the results. The agar plates were incubated at 37 °C for 24 h for Streptococcus mutans growth and then size of the inhibition zones was measured using vernier caliper. RESULTS: Amalgomer CR had shown significantly higher Compressive Strength but comparable antimicrobial efficay to GIC Fuji IX. CONCLUSION: Amalgomer CR can be recommended as a permanent restorative material based on its better compressive strength and comparable antimicrobial efficacy to GIC Fuji IX.

Dermatoglyphics as a Noninvasive Tool for Predicting Dental Caries in Cerebral Palsy and Healthy Children: An In Vivo Study.

AIM: To find the association of dermatoglyphics and dental caries in normal and cerebral palsy (CP) children. MATERIALS AND METHODS: A total of 150 children of age group 6-12 years were selected and divided into three equal groups. Group I constituted of 50 CP caries-active children, group II constituted of 50 healthy caries active children, and group III consisted of 50 healthy caries-free children. World Health Organization (WHO) criteria were used for diagnosis and recording of decayed, missing, filled teeth (DMFT)/deft scores. Fingerprints of both hands were taken using a stamp pad and analyzed using the Cummin and Midlo method. RESULTS: The results were statistically analyzed using one-way analysis of variance (ANOVA) and post hoc Tukey's honestly significant difference (HSD). The data for the entire study were calculated using statistical package for social sciences (SPSS) statistical software 19.0 version. The mean DMFT/deft score was the highest for the CP caries-active group compared to the healthy caries-active and healthy caries-free children. Dermatoglyphic pattern distribution in the CP caries-active group showed more whorls and that in the healthy caries-free group showed more arches. Intergroup comparisons for DMFT/deft and dermatoglyphic patterns were significant except between CP caries-active children and healthy caries-active children. CONCLUSION: Association of dermatoglyphics and dental caries was observed among CP caries-active children, healthy caries-active children, and healthy caries-free children. This association can be helpful in identifying the possible genetic predisposition and early prediction of dental caries in CP children, so as to initiate oral health measures at an early stage. CLINICAL SIGNIFICANCE: Maintaining oral hygiene has always been a challenge in children because of many difficulties like behavior management and lack of dexterity, and it becomes all the more difficult in the case of children with special needs. Dermatoglyphics can be proven to be a very useful, noninvasive, and economical tool for the preliminary diagnosis of diseases of suspected genetic origin like dental caries and CP. HOW TO CITE THIS ARTICLE: Somani R, Gupta MP, et al. Dermatoglyphics as a Noninvasive Tool for Predicting Dental Caries in Cerebral Palsy and Healthy Children: An In Vivo Study. Int J Clin Pediatr Dent 2019;12(3):237-242.

Hermetic Seal in Obturation: An Achievable Goal with Recently Introduced Cpoint.

AIM AND OBJECTIVES: To evaluate and compare the dentinal microcrack formation after obturation using CPoint and gutta percha as root canal filling materials. MATERIALS AND METHODS: Forty orthodontically extracted single-rooted premolars were selected. The teeth were decoronated and were then divided into four groups (n = 10). In group I, samples were left unprepared and unfilled (negative control), in group II, samples were prepared and left unobturated (positive control), in group III, samples were prepared and obturation was done with Cpoint (F3) and endosequence bioceramic sealer, and in group IV, samples were prepared and obturation was done with Protaper gutta percha (F3) and endosequence bioceramic sealer. Then, the samples were kept for 1 week at 37°C and 100% humidity. All roots were then sectioned at 2 mm from the apex. Additional cross sections at a 4 mm level were made in groups III and IV. Then, the sections were observed under a scanning electron microscope and the presence of dentinal microcracks was checked. RESULTS: There was statistically nonsignificant difference for a mean number of microcracks in the samples after obturation with CPoint and gutta percha. CONCLUSION: CPoint can be used as an alternative to gutta percha as it provides better seal because of its lateral hygroscopic expansion without causing significant damage to the dentinal microstructure. CLINICAL SIGNIFICANCE: In spite of gutta percha being the gold standard in obturating material, still the desired hermetic seal could not be achieved. To achieve this attainable goal, a new material CPoint had been introduced, which has the ability to undergo hygroscopic expansion within the root canal. HOW TO CITE THIS ARTICLE: Somani R, Jaidka S, Singh DJ, et al. Hermetic Seal in Obturation: An Achievable Goal with Recently Introduced Cpoint. Int J Clin Pediatr Dent 2019;12(5):410-413.

Comparative Microbiological Evaluation after Caries Removal by Various Burs.

AIM: The aim of the study was to compare the caries removal efficacy in terms of bacteriology and efficiency in terms of time taken by conventional and smart burs. MATERIALS AND METHODS: A total of 40 extracted permanent molars with occlusal caries were selected for this study. These teeth were split at the center of carious lesion buccolingually, in order to obtain two similar halves. Thus, 80 samples were obtained in this way and were randomly divided into 2 groups of 40 samples each. Caries was removed using conventional burs in group I and polymer bur Smartprep (SS white) in group II. The time involved in caries removal was measured for both the groups. After excavation of all carious lesions, the samples were decalcified, dehydrated, and embedded in paraffin wax from which thin sections of 5 µm were obtained, which were histologically evaluated for bacterial presence under a light microscope. RESULTS: An intergroup comparison between conventional bur (group I) and smart bur (group II) showed a statistically nonsignificant difference in terms of the presence of microorganisms after caries removal with a p value of 0.073638 (p > 0.05). However, the time taken for caries removal was significantly more for smart burs than diamond burs with a p value of 0.001 (p < 0.05). CONCLUSION: The polymer burs were found to be as effective as the conventional burs in terms of microbial presence after caries removal, but are more time-consuming than conventional burs. HOW TO CITE THIS ARTICLE: Somani R, Chaudhary R, Jaidka S, et al. Comparative Microbiological Evaluation after Caries Removal by Various Burs. Int J Clin Pediatr Dent 2019;12(6):524-527.

Comparative Evaluation of Efficiency of Different File Systems in Terms of Remaining Dentin Thickness Using Cone-Beam Computed Tomography: An In vitro Study.

BACKGROUND: Endodontic therapy treats inside of the tooth and its success is based upon the triad of thorough canal debridement, effective disinfection, and obturation of the canal space. Thus, one of the most important steps is biomechanical preparation, which is the key stage of endodontic treatment with a predictive success factor if performed properly. AIM: The aim of the study is to evaluate and compare the cleaning efficiency of different file systems in terms of remaining dentin thickness. MATERIALS AND METHODS: A total of thirty permanent extracted anterior teeth were taken for the study and was divided into three groups - Group I - Manual Protapers, Group II - Rotary Mtwo, and Group III - Reciprocating WaveOne file systems. Pre-cone-beam computed tomography (CBCT) scan was taken followed by biomechanical preparation by the respective file groups. Post-CBCT scan was taken and the pre- and post-CBCT scans were compared for remaining dentin thickness. Data were analyzed with ANOVA (P = 0.001). STATISTICAL ANALYSIS USED: ANOVA test was used in this study. RESULTS: Protaper showed minimum reduction in dentin thickness followed by Mtwo and WaveOne showed maximum reduction in dentin thickness, but the intergroup comparison was found to be highly insignificant. CONCLUSION: WaveOne is a highly recommended rotary endodontic instrument which does not cause significant lowering in remaining dentin thickness after biomechanical preparation.

Herbal combat against E. faecalis - An in vitro study.

BACKGROUND: Herbs have renewed importance in this modern era for their antimicrobial properties and fewer side effects. So a novel idea to use these herbs for pulpectomy; was implemented. There are chances of failure of pulpectomy due to inefficient removal of microbial load. Thus, for a successful endodontic treatment, obturating material with optimum antimicrobial properties is advocated in the present study. AIM: To compare and evaluate the antibacterial efficacy of zinc oxide eugenol, zinc oxide with tulsi extract and zinc oxide with aloe vera as obturating materials against E. faecalis bacteria. MATERIALS AND METHODS: Antimicrobial efficacy of zinc oxide eugenol as control, zinc oxide with tulsi extract and zinc oxide with aloe vera as experimental groups were assessed by using agar diffusion method. The statistical analysis was done. RESULTS: Intergroup comparison revealed significant difference amongst all the groups except between zinc oxide eugenol and zinc oxide with tulsi extract. Zinc oxide eugenol had significantly higher zone of inhibition among all the groups. CONCLUSION: According to results obtained from the present study can be summarized as follows: Zinc oxide eugenol > Zinc oxide with tulsi extract > Zinc oxide with aloe vera.

Miracle cells for natural dentistry - A review.

Stem cells are undifferentiated cells that can differentiate into specialized cells. Recently, enormous growth has been seen in the recognition of stem cell-based therapies, which have the potential to ameliorate the life of patients with conditions that span from Parkinson's disease to cardiac ischemia to bone or tooth loss. This research has produced new but unexplored possibilities in the regeneration of different organs and tissues. Presently, research is focused on the proficiency of stem cells and their utilization in dentistry, which is gaining interest. The tooth is nature's "esteem" for these precious stem cells and there are a number of these cells in permanent and primary teeth, as well as in the wisdom teeth. Dental stem cells are easy, convenient, and affordable to collect. They hold promise for a range of very potential therapeutic applications, such as in the treatment of cancer, spinal cord injury, brain damage, myocardial infarction, hearing loss, diabetes, wound healing, baldness, etc. Since these cells were used to regenerate damaged tissue in medical therapy successfully, it is possible that the dentist in future might use stem cell to regenerate lost or damaged dental and periodontal structures. This paper reviews the current concepts, characteristics of stem cells in regeneration, and its subsequent uses in dentistry.

Comparative Evaluation of Shear Bond Strength of Various Glass Ionomer Cements to Dentin of Primary Teeth: An in vitro Study.

AIM: To evaluate and compare shear bond strength of various glass ionomer cements (GICs) to dentin of primary teeth. MATERIALS AND METHODS: Sample size taken for the study was 72 deciduous molars with intact buccal or lingual surfaces. Samples were randomly divided into three groups, i.e., groups A, B, and C and were restored with conventional type II GIC, type II light cure (LC) GIC, and type IX GIC respectively. Thermocycling was done to simulate oral conditions. After 24 hours, shear bond strength was determined using Instron Universal testing Machine at crosshead speed of 0.5 mm/ minute until fracture. Results were tabulated and statistically analyzed. RESULTS: It was found that the shear bond strength was highest in group B (LC GIC) 9.851 ± 1.620 MPa, followed by group C (type IX GIC) 7.226 ± 0.877 MPa, and was lowest in group A (conventional GIC) 4.931 ± 0.9735 MPa. CONCLUSION: Light cure GIC was significantly better than type IX GIC and conventional GIC in terms of shear bond strength. HOW TO CITE THIS ARTICLE: Somani R, Jaidka S, Singh DJ, Sibal GK. Comparative Evaluation of Shear Bond Strength of Various Glass Ionomer Cements to Dentin of Primary Teeth: An in vitro Study. Int J Clin Pediatr Dent 2016;9(3):192-196.

Comparative evaluation of compressive strength, diametral tensile strength and shear bond strength of GIC type IX, chlorhexidine-incorporated GIC and triclosan-incorporated GIC: An in vitro study.

AIM: To comparatively evaluate the compressive strength, diametral tensile strength, and shear bond strength of glass ionomer cement type IX, chlorhexidine-incorporated glass ionomer cement, and triclosan-incorporated glass ionomer cement. MATERIALS AND METHODS: In this study, glass ionomer cement type IX was used as a control. Chlorhexidine diacetate, and triclosan were added to glass ionomer cement type IX powder, respectively, in order to obtain 0.5, 1.25, and 2.5% concentrations of the respective experimental groups. Compressive strength, diametral tensile strength, and shear bond strength were evaluated after 24 h using Instron Universal Testing Machine. The results obtained were statistically analyzed using the independent t-test, Dunnett test, and Tukey test. RESULTS: There was no statistical difference in the compressive strength, diametral tensile strength, and shear bond strength of glass ionomer cement type IX (control), 0.5% triclosan-glass ionomer cement, and 0.5% chlorhexidine-glass ionomer cement. CONCLUSION: The present study suggests that the compressive strength, diametral tensile strength, and shear bond strength of 0.5% triclosan-glass ionomer cement and 0.5% chlorhexidine-glass ionomer cement were similar to those of the glass ionomer cement type IX, discernibly signifying that these can be considered as viable options for use in pediatric dentistry with the additional value of antimicrobial property along with physical properties within the higher acceptable range.

Comparative evaluation of microleakage of newer generation dentin bonding agents: An in vitro study.

CONTEXT: Adhesive dentistry has been progressing with rapid pace over the past decade. Composite resin is the most esthetic restorative material currently available for restoring teeth. In spite of it being in use since a decade, still failure cases are seen which are mainly due to polymerization shrinkage and subsequent inadequate adhesion to cavity walls, leading to microleakage. Various generations of dentin bonding agents have been introduced to overcome the shortcoming of composite resin. AIMS: To determine the microleakage of the 6 th , 7 th , and 8 th generation dentin bonding agents. MATERIALS AND METHODS: Forty-five extracted human premolars were taken for the study. Standardized Class V cavities were prepared on all the teeth. The samples were divided into three groups according to the generation of bonding agent used. Group I was bonded with the 6 th generation, Group II with the 7 th generation, and Group III with the 8 th generation dentin bonding agent. All the Class V preparations were restored with a nano-ceramic composite restorative (Ceram X). The samples were then thermocycled between 5 and 55 ± 2°C for 100 cycles and immersed in 2% methylene blue for 48 h for evaluation of microleakage under a stereomicroscope. STATISTICAL ANALYSIS USED: The data was statistically analyzed using Kruskal-Wallis nonparametric analysis, and Mann-Whitney U-test was applied to compare the various groups. RESULTS: The microleakage value was the highest in Group II (7 th generation bonding agent) followed by Group I (6 th generation bonding agent) and least in Group III (8 th generation bonding agent). CONCLUSIONS: The 8 th generation dentin bonding showed statistically significant results in terms of lesser microleakage as compared to the 6 th and 7 th generation dentin bonding agents.

Evaluation of different Diagnostic Modalities for Diagnosis of Dental Caries: An in vivo Study.

AIM: The aim of this study was to compare and evaluate the efficacy of different diagnostic aids for diagnosis of dental caries and to compare the validity in terms of sensitivity and specificity of all four diagnostic modalities for diagnosis of caries. MATERIALS AND METHODS: Occlusal surfaces of 100 primary and permanent molars were examined using the four diagnostic systems (visual, intraoral camera, DIAGNOdent, and DIAGNOdent with dye). These results were compared with operative intervention gold standard. Sensitivity and specificity were calculated for each diagnostic system for both enamel and dentin caries. Interrater agreement was calculated for each diagnostic system using kappa statistics. RESULTS: For both enamel and dentin caries, the highest sensitivity values were provided by DIAGNOdent (0.91 and 0.72) and lowest for visual examination on wet surface (0.60 and 0.50). For both enamel and dentin caries, the specificity was found to be highest for intraoral camera on dry surface and lowest for visual examination. The DIAGNOdent gave the highest value of interrater agreement (kappa), i.e., 0.816 as compared with 0.03 for visual examination. CONCLUSION: The study clearly demonstrated that DIAGNO-dent was the most accurate and valid system tested for the detection of occlusal caries. It has the advantage of quantifying the mineral content, helping to improve the diagnostic efficacy and treatment and accurate assessment of fissures where the visual examination alone is not adequate, thus complementing the traditional dental examination. HOW TO CITE THIS ARTICLE: Zaidi I, Somani R, Jaidka S, Nishad M, Singh S, Tomar D. Evaluation of different Diagnostic Modalities for Diagnosis of Dental Caries: An in vivo Study. Int J Clin Pediatr Dent 2016;9(4):320-325.

Evaluation of antimicrobial efficacy of Aloe vera and Meswak containing dentifrices with fluoridated dentifrice: An in vivo study.

AIM: To comparatively evaluate the antimicrobial efficacy of fluoridated and herbal dentifrices. MATERIALS AND METHODS: Sixty students in the age group 6-12 years with DMF/def score 0 were selected from an orphanage center. The participants were divided into four groups. In group A, no dentifrice was used; in group B, fluoride containing dentifrice was used; group C subjects used Aloe vera containing dentifrice; and in group D, Meswak containing dentifrice was used. The salivary samples were collected at the washout period of 2 days, 15 days, and 30 days and cultured on Mitis Salivarius Agar for determining Streptococcus mutans count. Results obtained were statistically analyzed using Student's t-test. RESULTS: There was an increase in bacterial count in group A where no dentifrices were used, while the bacterial count steadily decreased in groups B, C, and D by 83.7%, 80.94%, and 83.5%, respectively. CONCLUSION: Herbal dentifrices containing A. vera and Meswak can be safely recommended as an alternative to fluoridated dentifrices in terms of antimicrobial efficacy.

Comparative evaluation of smear layer removal by various chemomechanical caries removal agents: an in vitro SEM study.

OBJECTIVES: To comparatively evaluate the efficiency of various chemomechanical caries removal agents in smear layer removal. MATERIALS AND METHODS: Class V cavities were prepared on the buccal surface of 45 extracted, noncarious molars and were divided into three groups according to the chemical treatment received. The samples were then gold palladium coated and viewed under scanning electron microscope. The presence of smear layer was evaluated based on the microscopic observations. Result and Observation: The results indicated that smear layer removal was significantly higher in the sodium hypochlorite-based chemomechanical caries removal agents than in the papain-based chemomechanical caries removal agent and was least in the control group treated with saline. CONCLUSION: The smear layer removal was maximum with Carisolv followed by the cariecare and was minimum in the saline-treated group.

Comparative evaluation of microleakage in conventional glass ionomer cements and triclosan incorporated glass ionomer cements.

AIM AND OBJECTIVE: The aim of the following study is to comparatively evaluate the microleakage of triclosan incorporated GIC with conventional restorative GIC. MATERIALS AND METHODS: Triclosan in powder form was added to conventional GIC to formulate a concentration of 2.5%. Class five cavities were prepared in non-carious extracted molars and were respectively restored with conventional restorative GIC and triclosan incorporated GIC. Samples were kept in 10% methylene blue dye. Ground sections were obtained and were observed under a binocular microscope for dye penetration. RESULT: No significant difference was found in the microleakage of two groups. CONCLUSION: Triclosan incorporated GIC can be considered as an alternative to GIC with enhanced antibacterial property.

Remineralizing potential of various agents on dental erosion.

AIM: The purpose of this study is to compare the effect of casein phosphopeptide-amorphous calcium phosphate (CPP-ACP, Tooth Mousse) containing and casein phosphopeptide-amorphous calcium phosphate with fluoride (CPP-ACPF, Tooth Mousse Plus) containing pastes on dental erosion. MATERIALS AND METHODS: Thirty permanent non-carious premolars indicated for orthodontic extraction were included in this study and were sectioned in mesiodistal direction vertically. After immersion in the carbonated drink for 14 min, samples were treated with various remineralizing pastes which were CPP-ACP containing paste (Tooth Mousse) and CPP-ACPF containing paste (Tooth Mousse Plus) according to the manufacturer's instructions. Vickers Microhardness was recorded at baseline, after exposure to erosive drink and after treatment with remineralizing pastes. Data obtained was statistically analysed using Student t-test with a level of significance set at p < 0.05. RESULTS: CPP-ACP (Tooth Mousse) and CPP-ACP with fluoride (Tooth Mousse Plus) resulted in 30.52% and 38.98% increase in post-erosion microhardness values respectively. The remineralizing potential of CPP-ACP with fluoride containing paste (Tooth Mousse Plus) was significantly better than that of CPP-ACP containing paste (Tooth Mousse) (p < 0.05). CONCLUSION: Casein phosphopeptide-amorphous calcium phosphate with fluoride (CPP-ACPF, Tooth Mousse Plus) can be recommended to be used in preventing erosive tooth wear from acidic beverages.

When Nature plays upon an Ailment: A Case Report.

Of the most common odontogenic cysts, radicular cysts represent cystic lesions of inflammatory origin and are managed either by surgical enucleation or by marsupialization. This article aims to report a clinical case of radicular cyst of a huge proportion treated with a conservative management. An illustration of possible complete healing of such a cystic periapical lesion in mixed dentition with conservation of vital structures is covered. How to cite this article: Somani R, Garewal R, Bhandari PP, Kumar D. When Nature plays upon an Ailment: A Case Report. Int J Clin Pediatr Dent 2012;5(1):61-63.

Comparative clinical evaluation of chemomechanical caries removal agent Papacarie® with conventional method among rural population in India: in vivo study

The use of minimally invasive procedures and attention to patient comfort are of great importance, especially for dental treatment in young children. This has led to the development of chemomechanical methods for caries removal. Aim: To evaluate and compare the antimicrobial efficacy, efficacy in terms of time consumption and pain perception of chemomechanical caries removal agent Papacarie® and conventional method of caries removal. Methods: Subjects for this study were chosen from children admitted to dental clinic for restorative procedures. Forty children (age 4-8 years) with early childhood caries were included in this study. Two primary teeth with comparable degrees of carious destruction were chosen in each child for caries removal with either Papacarie® or rotary instruments. The time taken for caries removal was measured using stopwatch. Pain response during caries removal was evaluated using the Wong Baker Face Pain Scale. Dentin samples of both groups were taken prior to, and after caries removal for microbiological analysis. Results The time taken for caries removal in chemomechanical caries removal method was three times longer than the conventional method. Pain score during chemomechanical method of caries removal was 1.525 as compared with 6.65 when conventional method was used. The antimicrobial efficacy of chemomechanical caries removal was significantly similar to conventional method. Conclusions: Papacarie® can be an effective clinical alternative treatment for the removal of occlusal dentinal caries in cavitated primary molars.

Platelet Rich Plasma-A Healing Aid and Perfect Enhancement Factor: Review and Case Report.

Platelet rich plasma (PRP) has been a breakthrough in the stimulation and acceleration of tissue healing. It represents a relatively new approach in regenerative procedures and is a developing area in pediatric dentistry. It is an autologous source of growth factors obtained by sequestrating and concentrating platelets by gradient density centrifugation. This novel and potentially promising technique enhances body's natural wound healing mechanism. This article goes on to describe preparation and clinical benefits of PRP in pediatric dentistry.