ABSTRACT
We report the prevalence of extended-spectrum ß-lactamases and carbapenemases in Escherichia coli isolated from retail chicken in Peshawar, Pakistan. One hundred E. coli isolates were recovered from retail chicken. Antibiotic susceptibility testing was carried out against ampicillin, chloramphenicol, kanamycin, nalidixic acid, cephalothin, gentamicin, sulfamethoxazole-trimethoprim, and streptomycin. Phenotypic detection of ß-lactamase production was analyzed through double disc synergy test using the antibiotics amoxicillin-clavulanate, cefotaxime, ceftazidime, cefepime, and aztreonam. Fifty multidrug-resistant isolates were screened for detection of sul1, aadA, cmlA, int, blaTEM, blaSHV, blaCTX-M, blaOXA-10, blaVIM, blaIMP, and blaNDM-1 genes. Resistance to ampicillin, nalidixic acid, kanamycin, streptomycin, cephalothin, sulfamethoxazole-trimethoprim, gentamicin, cefotaxime, ceftazidime, aztreonam, cefepime, amoxicillin-clavulanate, and chloramphenicol was 92, 91, 84, 73, 70, 67, 53, 48, 40, 39, 37, 36, and 23% respectively. Prevalence of sul1, aadA, cmlA, int, blaTEM, blaCTX-M, blaIMP, and blaNDM-1 was 78% ( n = 39), 76% ( n = 38), 20% ( n = 10), 90% ( n = 45), 74% ( n = 37), 94% ( n = 47), 22% ( n = 11), and 4% ( n = 2), respectively. blaSHV, blaOXA-10, and blaVIM were not detected. The coexistence of multiple antibiotic resistance genes in multidrug-resistant strains of E. coli is alarming. Hence, robust surveillance strategies should be developed with a focus on controlling the spread of antibiotic resistance genes via the food chain.
Subject(s)
Bacterial Proteins/metabolism , Chickens/microbiology , Drug Resistance, Multiple, Bacterial , Escherichia coli , beta-Lactamases/metabolism , Animals , Anti-Bacterial Agents/pharmacology , Escherichia coli/drug effects , Escherichia coli/enzymology , Food Microbiology , PakistanABSTRACT
Tuberculosis is a major infectious disease caused by Mycobacterium tuberculosis complex. Antimicrobial drugs are used to control TB infections. Molecular mechanisms controlling resistance to second-line drugs are not completely understood and no endogenous information is available regarding these mechanisms. The present study reports mutational analysis of rrs gene in Mycobacterium tuberculosis isolates collected from Khyber Pakhtunkhwa province of Pakistan. A total of 499 Mycobacterium tuberculosis isolates were analyzed for resistance against amikacin. Thirty resistant isolates were selected for mutational analysis in rrs gene. Among the 30 amikacin resistant isolates of Mycobacterium tuberculosis, 9 (30%) had mutation in the hotspot region of rrs gene. The predominant mutation was 1401A > G which was observed in 5 isolates. Maximum number of mutations was observed in isolate 6 and isolate 16 with six different mutations each. Mutations in isolate 6 included 1260G > A, 1278A > T, 1278_1279insT, 1300C > T, 1321G > A and 1445C > T. Mutation in isolate 16 included 1255_1256insA, 1364_1365insG, 1384_1385insA, 1880_1881insT, 1487G > A, and 1493delA. The mutation 1263G > A was observed in isolate 1. Isolate 2 had the 1484G > T mutation. The findings could be used as reference for future endures. It was evident from the results that mutations in rrs gene do not always contribute to amikacin resistance; hence, traditional drug susceptibility testing is still helpful for evaluation of such samples.
Subject(s)
Antitubercular Agents/pharmacology , Drug Resistance, Bacterial/genetics , Mutation , Mycobacterium tuberculosis/drug effects , Mycobacterium tuberculosis/genetics , RNA, Ribosomal, 16S/genetics , Tuberculosis, Multidrug-Resistant/microbiology , Amikacin/pharmacology , Bacterial Proteins/genetics , Base Sequence , DNA Mutational Analysis , DNA, Bacterial , Genes, Bacterial/genetics , Humans , Microbial Sensitivity Tests , Mycobacterium tuberculosis/isolation & purification , PakistanABSTRACT
OBJECTIVE: To report some of the pharmacological features of the medicinal plant Ocimum basilicum. METHODS: In the current studies, Antifungal activity of crude methanolic fraction of Ocimum basilicum was determined against eight pathogenic fungal strains using tube dilution assay. The methanolic fraction was also investigated for phytotoxic and hemagglutination activity. RESULTS: Of the eight strains investigated only Candida albicans and Curvilaria lunata were found to be least affected by plant extract while the rest were significantly inhibited. Moderate phytotoxic activity was observed against lemna minor. Hemagglutination activity showed absence of phytolectins and hence no agglutination of erythrocytes. CONCLUSION: The crude extract of Ocimum basilicum has significant properties against fungi and phytotoxic substances.
