The Scattered Light Time-history Diagnostic suite at the National Ignition Facility.

The Scattered Light Time-history Diagnostic (SLTD) is being implemented at the National Ignition Facility (NIF) to greatly expand the angular coverage of absolute scattered-light measurements for direct- and indirect-drive inertial confinement fusion (ICF) experiments. The SLTD array will ultimately consist of 15 units mounted at a variety of polar and azimuthal angles on the NIF target chamber, complementing the existing NIF backscatter suite. Each SLTD unit collects and diffuses scattered light onto a set of three optical fibers, which transport the light to filtered photodiodes to measure scattered light in different wavelength bands: stimulated Brillouin scattering (350 nm-352 nm), stimulated Raman scattering (430 nm-760 nm), and ω/2 (695 nm-745 nm). SLTD measures scattered light with a time resolution of ∼1 ns and a signal-to-noise ratio of up to 500. Currently, six units are operational and recording data. Measurements of the angular dependence of scattered light will strongly constrain models of laser energy coupling in ICF experiments and allow for a more robust inference of the total laser energy coupled to implosions.

Comparison of hepatic lesions in veal calves with concentrations of copper, iron and zinc in liver and kidney.

Veal calf producers in Indiana have reported condemnation of carcasses due to icterus as well as condemnation of livers because of yellow discoloration, hepatomegaly and fibrosis. This study assessed the degree of hepatic injury in affected veal calves and correlated it with copper, iron and zinc concentrations in the liver and kidney. Tissues examined histopathologically were from slaughtered and necropsied veal calves. Hepatic lesions were divided into histopathologic categories of severity (minimal, moderate, marked or severe) based upon the degree of fibrosis, biliary epithelial hyperplasia, and inflammation. Hepatic copper levels decreased as the severity of lesions increased. The clinical observations and morphologic changes suggested initial hepatic damage before 9 w-of-age. The affected calves either died of acute copper toxicosis or survived to develop hepatomegaly, hepatic discoloration and/or fibrosis at the time of slaughter.

Some relationships between skills in word-category recall and factors in adults' aphasia.

It has long been recognized that a basic dimension to the lexical organization of the brain is semantic, and some brain mapping studies have indicated that the brain fields are distinctly different from some grammatical classes. Findings from the present investigation showed consistent relationships between 29 aphasic adults' performances on tasks involving graphic and gestural skills and those involving sequential recall of spoken words from different word categories. Each adult received the Porch Index of Communication Abilities which relies upon the physical manipulation of objects to assess verbal, gestural, and graphic abilities. Scores on a test requiring recall of word strings of nouns, verbs, adverbs, adjectives, or prepositions were used to predict the subscale scores from the Graphic and Gestural factors of the index. Recall scores for verb and preposition were predictive of the aphasic subjects' performances on the Graphic subscale, and noun and preposition scores were predictors of subjects' scores on the Gestural subscale. The results are related to other research showing that verb and preposition skills are predictive of fine motor abilities of children with communication disorders and brain-mapping studies. Some discussion centers on possible overlapping functions of brain activity involving word categories, language, and fine motor skills.

Training profoundly retarded adults to communicate using handguiding.

Four multiply disabled, profoundly retarded young adults received two series of instructional activities designed to teach them to use handguiding to communicate basic needs and intents. All had failed in previous attempts to learn to communicate using augmentative devices and strategies. Caregivers and staff served as partners in communication. Three of the four subjects were successful in learning some basic communication skills using the strategy of having a partner and handguiding as a technique.

Simultaneous assessment of loss of heterozygosity at multiple microsatellite loci using semi-automated fluorescence-based detection: subregional mapping of chromosome 4 in cervical carcinoma.

Detection of loss of heterozygosity (LOH) by comparison of normal and tumor genotypes using PCR-based microsatellite loci provides considerable advantages over traditional Southern blotting-based approaches. However, current methodologies are limited by several factors, including the numbers of loci that can be evaluated for LOH in a single experiment, the discrimination of true alleles versus "stutter bands," and the use of radionucleotides in detecting PCR products. Here we describe methods for high throughput simultaneous assessment of LOH at multiple loci in human tumors; these methods rely on the detection of amplified microsatellite loci by fluorescence-based DNA sequencing technology. Data generated by this approach are processed by several computer software programs that enable the automated linear quantitation and calculation of allelic ratios, allowing rapid ascertainment of LOH. As a test of this approach, genotypes at a series of loci on chromosome 4 were determined for 58 carcinomas of the uterine cervix. The results underscore the efficacy, sensitivity, and remarkable reproducibility of this approach to LOH detection and provide subchromosomal localization of two regions of chromosome 4 commonly altered in cervical tumors.

Analysis of replication error (RER+) phenotypes in cervical carcinoma.

Infection of epithelial cells with human papillomavirus is an important early event in the development of cervical dysplasia. However, progression to overt malignancy appears dependent upon further genetic and/or epigenetic events. We have recently developed methodologies for the simultaneous analysis of loss of heterozygosity (LOH) at multiple PCR-based microsatellite loci using semiautomated fluorescent DNA sequencing technology to determine the locations of tumor suppressor genes which are inactivated during tumor progression. While examining 30 microsatellite loci for LOH on chromosomes 3p, 4, and 11q, we detected novel tumor-specific alleles indicative of microsatellite instability (MI). The methodology allowed rapid and accurate comparison of over 3000 genotypes from 89 primary tumors and 10 cervical carcinoma-derived cell lines and showed that five tumors (5.6%) and one human papillomavirus-negative cell line, C33A, had genetic features consistent with a replication error (RER+) phenotype as defined by MI at two or more loci. In each of the RER+ tumors, LOH was also observed at one or more loci on each of the three chromosomes examined. These findings suggest that defects in DNA repair-associated genes are rarely acquired and do not supersede allelic loss during cervical carcinogenesis. In addition, the semiautomated multiplex approach has proven unequivocal in the detection and interpretation of MI and should greatly accelerate the rapidity and accuracy of analysis of such defects in tumors. Moreover, the number of loci that can be relatively easily examined in this way will also allow a detailed statistical consideration of the importance of such events.

Changes in maternal attitudes and perceptions and children's communication skills.

The present investigation had two purposes: (a) to assess significant changes in the attitudes and perceptions of mothers of young children who were enrolled in 15 wk. of individual speech-language therapy and (b) to compare changes in mothers' attitudes and perceptions with university supervisors' ratings of children's over-all communication skills and speech intelligibility. 17 children, ages 2;10 to 5;8, and their mothers were participants. Mothers received speech-language services and counseling from graduate-student clinicians and university clinical supervisors but no special counseling was provided to alter their attitudes and perceptions concerning the intervention process. The typical child in the intervention program received 24 individual speech and language therapy sessions. Mothers and supervisors completed questionnaires before and after the 15-wk. intervention. Neither group was made aware of the purpose of the investigation. Analysis showed a pattern of significant changes in mothers' pre- and postrankings of questionnaire items and significant changes in the total communication and speech intelligibility rankings made by supervisors. Significant relationships were found for changes in attitudes and perceptions of mothers and changes in the children's communication skills. The results bolster the need for use of family-based therapy approaches in intervention programs for young children's communication disorders.

Word skills of children normal and impaired in communication skills and measures of language and speech development.

Results from two related investigations are reported, one using 60 normal language learners, ages 3-5 and one using 34 children with communication disorders, ages 5.4-8.5. Tasks involving the sequential recall of words from five categories (nouns, verbs, adjectives, adverbs, and prepositions) were given to the subjects in each investigation, thus providing an opportunity to compare group performances. A hierarchy of recall strengths of words from different categories and the organizational pattern of scores was determined for each group. Each group's word category scores were used as independent variables in regression analyses to predict scores from a battery of language tests and a test of phonology. The results were anticipated to be capable of contributing to descriptions of children's mental dictionaries, have implications for word category differences in normal and impaired language learners, and clinical relevance. Comparisons of the word recall accuracy of skills of children from each group revealed that the younger normal Ss had word scores equal to the older language and speech impaired children. However, the hierarchy of word category strengths and the patterns of organization within each group's mental dictionary were essentially the same in both groups. Word category scores predicted language and speech scores in both investigations, but the prediction was stronger in the children having impaired language or speech.

Adults who stutter: responses to cognitive stress.

The purpose of this study was to investigate the effects of speed and cognitive stress on the articulatory coordination abilities of adults who stutter. Cardiovascular (heart rate, systolic blood pressure, and diastolic blood pressure), behavioral (dysfluencies, errors, speech rate, and response latency), and acoustic (word duration, vowel duration, consonant-vowel transition duration/extent, and formant center frequency) measures for nine stutterers and nine nonstutterers were collected during performance of the Stroop Color Word task, a well-established and highly stressful cognitive task. Significant differences were found between the two groups for heart rate, word duration, vowel duration, speech rate, and response latency. In addition, stutterers produced more dysfluencies under speed plus cognitive stress versus speed stress or a self-paced reading task. These findings demonstrate that the presence of cognitive stress resulted in greater temporal disruptions and more dysfluencies for stutterers than for nonstutterers. However, similar spatial impairments were not evident. The potential contributions of the Stroop paradigm to stuttering research as well as the need for further research on autonomic correlates of stuttering are also discussed.

Serial serum CA 125 measurements for evaluation of recurrence in patients with endometrial carcinoma.

OBJECTIVE: To evaluate the usefulness of serum assays for CA 125 to detect recurrent endometrial carcinoma. METHODS: Two hundred sixty-six patients were studied with 1101 post-treatment assays. Patients were categorized as low, medium, or high risk based on surgical-pathologic findings. CA 125 values were analyzed with respect to each patient's disease status. RESULTS: Serial CA 125 levels were elevated (greater than 35 U/mL) in 19 of 33 patients (58%) with recurrent disease. Among 236 surgically treated patients, 97 (41.1%), 42 (17.8%), and 97 (41.1%) were considered low, medium, and high risk, respectively. None of the low-risk and only two (4.7%) of the medium-risk patients developed recurrent disease. One of the latter patients was detected based on an elevated CA 125 level alone. Twenty-seven (27.8%) of the high-risk patients developed recurrent disease, 23 of whom had elevated pre-treatment CA 125. Fifteen of 16 (94%) with recurrent disease had an elevated CA 125 level. Nine of 12 patients with papillary serous carcinoma experienced recurrence; eight of these nine had elevated CA 125 levels at diagnosis and recurrence, in contrast to only one patient with a normal pre-treatment level (P = .018). False elevations were noted in 13 patients, 12 of whom had received radiation therapy. CONCLUSIONS: CA 125, if elevated at diagnosis of endometrial carcinoma, is an important marker for recurrent disease. The use of serial CA 125 assays is most beneficial in diagnosing recurrence in a high-risk population, including patients with papillary serous carcinomas. False elevations may occur following radiation therapy.

Contributions of the fundamental, resolved harmonics, and unresolved harmonics in tone-phoneme identification.

Researchers describe Mandarin Chinese tone phonemes by their fundamental frequency (Fo) contours. However, tone phonemes are also comprised of higher harmonics that also may cue tone phonemes. We measured identification thresholds of acoustically filtered tone phonemes and found that higher harmonics, including resolved harmonics above the Fo and unresolved harmonics, cued tone phonemes. Resolved harmonics cued tone phonemes at lower intensity levels suggesting they are more practical tone-phoneme cues in everyday speech. The clear implication is that researchers should use the Fo only as a benchmark when describing tone-phoneme contours, recognizing that higher harmonics also cue tone phonemes. These results also help explain why tone-language speakers can identify tone phonemes over a telephone that attenuates selective frequencies, and suggests that hearing-impaired tone-language speakers may still identify tone phonemes when their hearing loss attenuates selective frequencies.

A review and critical analysis of treatment research related to articulation and phonological disorders.

This article contains a summary of aspects of research designs and strategies found in 63 published reports in which the effectiveness of treatment of articulation or phonological disorders was evaluated. These research reports were published in four nationally refereed journals that contained most of the literature published in the decades of the 1970s and 1980s. A total of 91 items were evaluated in each report by two reviewers working independently, including types of research designs, details about subjects, sampling, and types of independent and dependent variables used by researchers. Comparisons were made within each decade and across both decades to identify strengths and limitations. Some significant differences in research designs and variables under investigation occurred between the decades. A critical analysis was performed, and suggestions for changes are discussed.

Renal excretion of perfluorooctanoic acid in male rats: inhibitory effect of testosterone.

There is a marked sex difference in the whole-body elimination of perfluorooctanoic acid (PFOA) in rats, with females excreting the perfluorinated acid much more rapidly (half life [t1/2] less than 1 day) than males (t1/2 = 15 days). Our objective was to determine if androgens or estrogens are involved in causing this sex difference in PFOA elimination. Castration of males greatly increased the elimination of [1-14C]PFOA (9.4 mumol/kg, i.p.) in urine, demonstrating that a factor produced by the testis was responsible for the slow elimination of PFOA in male rats. Castration plus 17 beta-estradiol had no further effect on PFOA elimination whereas castration plus testosterone replacement at the physiologic level reduced PFOA elimination to the same level as rats with intact testes. Thus, in male rats, testosterone exerts an inhibitory effect on renal excretion of PFOA. In female rats, neither ovariectomy nor ovariectomy plus testosterone affected the PFOA urinary elimination, demonstrating that the inhibitory effect of testosterone on PFOA renal excretion is a male-specific response. Probenecid decreased the high rate of PFOA renal excretion in castrated males but had no effect on male rats with intact testes. We conclude that testosterone is a key determinant of the sex difference in PFOA elimination in rats.

Approaches to the prediction of language abilities in a sample of children who have developmental delays.

Prediction of the quality of language was explored using planned comparisons of three approaches, one cognitive, one neurodevelopmental, and one a combination of the two. Subjects were 37 children, ages 5-9 years, whose significant developmental delays included language and speech skills. The cognitive predictors were mental age (MA) and IQ from the Stanford-Binet Intelligence Scale. Neurodevelopmental predictors consisted of fine motor skill quotients (MQs) and dichotic speech processing scores. Chronological age (CA) was also evaluated as a predictor. A composite language ability score constituted the dependent variable. Results of regression analyses showed that CA and MQ, and MA and MQ, were nearly equal in their predictive strengths and were substantial predictors of composite language scores. Larger multiple correlations (low .8 range) were found when combinations of MA, IQ, and MQ or CA, IQ, and MQ were used as predictors. Statistical control over the 4-year age range revealed that approximately equal amounts of prediction of language scores were attributable to CA and a combination of MA, IQ, and MQ. Each of the latter variables contributed important amounts of unique variance to the language score prediction. Dichotic ear scores did not relate to cognitive or language scores and were ineffectual as predictors in regression analysis. Results indicated that children of the type studied have language and speech delays that show substantial relationships to their verbal cognitive abilities and MQs, in addition to their CAs.

Off-site storage--move it!

The planning and implementation of the onsite proposal required the devotion and creativity of many individuals, including administrative, clerical, and line workers, and has been accomplished through the completion of a series of objectives. While the plan took three years to be realized, cost containment efforts of this magnitude signify the important contribution a material management department can make in the healthcare industry. Departmental credibility and support from customers are the foundation for implementing such a plan in any medical center. Materiel management departments continue to incorporate creative and innovative programs into their daily operations, which contributes to the institution's bottom-line. This on-site project has brought The University of Michigan Hospitals one step closer to its ultimate cost containment goal of a total stockless purchasing/supply program.

Unisensory and bisensory processing skills of children having misarticulations and normally speaking peers.

The purpose of this investigation was to test the developmental delay hypothesis as a possible explanation for the existence of misarticulations in 7- and 8-year-old children. To accomplish this, 10 normally speaking children, 10 having mild misarticulations, and 10 children with severe misarticulations were administered unisensory and bisensory processing tasks. Unisensory tasks consisted of oral and manual form discrimination and auditory recall of word strings. Bisensory tasks were combinations of the unisensory ones and were of major interest, because this form of processing has been considered a test of children's CNS maturity. Results showed that children having misarticulations obtained lower scores than children with normal articulation on all bisensory tasks and had larger decrements from unisensory to bisensory tasks than control subjects. On unisensory tasks involving recall of word strings and manual form discrimination, differences were also found in favor of the control subjects. The results supported the hypothesis that delayed CNS development may coexist with misarticulations of 7- and 8-year-old children.

Prediction of fine motor skills of children having language and speech disorders.

Relationships between the fine motor skills and linguistic abilities of 37 developmentally delayed children, ages 5 to 9 yr., were studied using a battery of expressive and receptive language tests, a measure of fine motor performance, a dichotic listening test, and individual intelligence tests. While IQs and MAs were not related to fine motor skills, both expressive and receptive language test scores showed moderate to moderately high correlations, the highest single relationship being the Test for the Auditory Comprehension of Language. In concert with CAs, a dichotic right-ear test score, the Auditory Comprehension Test, predicted fine motor-skill indices substantially; R = .80. Strong relationships appear between linguistic and fine motor skills in an age group not previously investigated and at higher levels than reported in studies of infants and very young children. Dichotic results were abnormal in a majority of the children.

A hospital-wide forms management program: doing more for less.

The day-to-day management of the hospital forms business can be time-consuming, frustrating, and expensive. In many cases, the official inventory represents only a fraction of the total number of forms used throughout an institution. Since the responsibility for inventory and quality control becomes diffuse, forms users experience frequent stockouts and poorly designed forms. Without a centralized program, it is difficult to determine the costs associated with forms. An effective forms management program can be designed to fulfill most everyone's needs. The program described in this article incorporates the concept of stockless purchasing to gain better quality forms at significant cost savings.

Nonspeech and speech processing skills in patients with aphasia and apraxia of speech.

Nonspeech and speech auditory processing skills as well as internal speech processing skills were assessed among four patients with acquired "pure" apraxia of speech, 10 with acquired aphasia, 10 with aphasia plus apraxia of speech, and 11 neurologically normal adults. Fourteen tasks were administered and performances on 68 variables were examined using both nonparametric and parametric analyses controlling for the effects of advancing age and associated hearing loss. In all cases, the "pure" apractic patients performed as normal subjects. Few differences were noted among the performances of the aphasic and aphasic-apractic subjects. Results led to three major conclusions: (1) apraxia of speech is a disorder distinct from aphasia; (2) aphasic individuals, despite locus of lesion, demonstrate disabilities for processing nonspeech and speech materials presented auditorily and for analytically evaluating speech evoked internally; and (3) aphasic individuals demonstrating similar severity levels of language impairment show similar performance patterns for these types of processing tasks, despite locus of lesion or coexistence of apraxia of speech.