ABSTRACT
In order to increase the solubility of PEDOT, we introduced an alkyl chain into polymer backbone. Depending on the position of the alkyl bond, the steric hindrance between the alkyl chains can also leads to regioregularity in the polymer. The regioregular polymers can easily occur self-assembly, both in solution and in the solid state, resulting in highly ordered two- and three-dimensional polymer architectures. A series of soluble regioregular alkyl-substituted PEDOTs were synthesized using the Grignard Metathesis (GRIM) for the purpose of study on regiorandom and regioregular PEDOT. The ethylene proton adjacent to the oxygen atom of regioregular methoxydodecyl-PEDOT appears at 3.51 ppm, whereas the corresponding peak of regiorandom methoxydodecyl-PEDOT appears at 3.42 ppm.
ABSTRACT
The regioregular hexyl-substituted poly(3,4-ethylenedioxy-2-thiophenylthiophene) (PEDOTt), structurally alternating copolymer of EDOT and thiophene, was synthesized via the Grignard Metathesis (GRIM) using catalytic amounts of Ni(dppp)Cl2. The products were characterized by 1H NMR spectroscopy, UV-vis spectroscopy and XRD. The alkyl-substituted conjugated polymer showed an enhancement of solubility in common organic solvents. Based on XRD data, the regioregular hexyl-substituted poly(3,4-ethylenedioxy-2-thiophenylthiophene) (PEDOTt) shows better crystallinity than the regiorandom hexyl-substituted PEDOTt owing to self-ordering. In addition, regioregular hexyl-PEDOTt has a conductivity of 0.788 S/cm by iodine doping.
ABSTRACT
Orientation of antibodies is very important in the preparation of immunoarrays to keep the activity of antibodies on solid surfaces. Thus, we synthesized a new bifunctional compound, 2-(biotinamido)ethanethiol, and investigated whether the thiol compound is useful to analyze antibody-antigen interactions on immunoarrays with a spectral SPR biosensor. The synthesized organic thiol was characterized by nuclear magnetic resonance spectroscopy and mass spectrometry. 2-(Biotinamido)ethanethiol formed a monolayer on a gold surface and properly immobilized antibodies via streptavidin and biotinylated protein G. Optimal molar ratio of 2-(biotinamido)ethanethiol and mercaptohexanol for antigen-antibody interactions was 1:2. Thus, 2-(biotinamido)ethanethiol is an useful bifunctional linker in the preparation of immunoarrays on gold surfaces.
