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1.
Food Chem Toxicol ; 74: 255-64, 2014 Dec.
Article in English | MEDLINE | ID: mdl-25447760

ABSTRACT

Genistein was irradiated with γ-irradiation at doses of 0, 10, 30, 50, 100, and 150 kGy. We observed that the decrease in the genistein peak after gamma irradiation was concomitant with the appearance of several new peaks. 150 kGy gamma-irradiated genistein did not exert cytotoxicity in macrophages, and inhibited inducible nitric oxide synthase-mediated nitric oxide production and pro-inflammatory cytokines level, such as tumor necrosis factor-α, interleukin-6 and interleukin-1ß, in lipopolysaccharide (LPS)-induced macrophages. The treatment of LPS-stimulated macrophages with 150 kGy gamma-irradiated genistein resulted in a significant decrease in cyclooxygenase-2 levels, as well as the expression of cell surface molecules, such as CD80 and CD86. Furthermore, we also found that the anti-inflammatory action of 150 kGy gamma-irradiated genistein occurred through an inhibition of mitogen-activated protein kinases (extracellular signal-regulated kinase 1/2, p38 and c-Jun N-terminal kinase) and nuclear factor-κB signaling pathways based on a toll-like receptor 4 in macrophages, which may be speculated that several radiolysis products of genistein transformed by gamma-irradiation induce the inhibition of pro-inflammatory mediators. From these findings, it seems likely that gamma-irradiated genistein could play a potent role in the treatment of inflammatory disease as a value-added product in the medical industry.


Subject(s)
Anti-Inflammatory Agents/pharmacology , Anti-Inflammatory Agents/radiation effects , Genistein/pharmacology , Genistein/radiation effects , Lipopolysaccharides/toxicity , MAP Kinase Signaling System/drug effects , Macrophages/drug effects , NF-kappa B/antagonists & inhibitors , Animals , Anti-Inflammatory Agents/chemistry , B7-1 Antigen/biosynthesis , B7-2 Antigen/biosynthesis , Cell Line , Cell Proliferation/drug effects , Functional Food , Gamma Rays , Genistein/chemistry , Mice , Nitric Oxide/metabolism , Toll-Like Receptor 4/drug effects
2.
Immunobiology ; 219(11): 866-72, 2014 Nov.
Article in English | MEDLINE | ID: mdl-25109435

ABSTRACT

BACKGROUND: Green tea polyphenol epigallocatechin-3-gallate (EGCG) has the potential to impact a variety of inflammation-related diseases; however, the anti-inflammatory action of EGCG in endothelial cells has not been understood. Recently, we demonstrated that the 67-kDa laminin receptor (67LR) acts as a cell-surface EGCG receptor. AIM: This research was carried out to clarify the molecular basis for the down-regulation of toll-like receptor 4 (TLR4) signal transduction by EGCG in lipopolysaccharide (LPS)-stimulated endothelial cells. RESULTS: RNAi-mediated silencing of 67LR resulted in an abrogation of the inhibitory action of EGCG on the LPS-induced activation of downstream signaling pathways. Also, we found that EGCG induced a rapid upregulation of Toll-interacting protein (Tollip), a negative regulator of TLR signaling, through 67LR in endothelial cells. RNAi-mediated silencing of Tollip impaired the TLR4 signaling inhibitory activity of EGCG. Additionally, silencing of Tollip resulted in an abrogation of the inhibitory action of EGCG on the LPS-induced expressions of cell-associated adhesion molecules, such as ICAM-1 and VCAM-1. CONCLUSION: Taken together, these novel findings provide new insights into an understanding of negative regulatory mechanisms of the TLR4 signaling pathway and effective therapeutic intervention for the treatment of inflammatory disease.


Subject(s)
Catechin/analogs & derivatives , Endothelial Cells/metabolism , Intracellular Signaling Peptides and Proteins/metabolism , Receptors, Laminin/metabolism , Signal Transduction/drug effects , Toll-Like Receptor 4/metabolism , Animals , Catechin/pharmacology , Cell Adhesion Molecules/genetics , Cell Adhesion Molecules/metabolism , Gene Expression Regulation/drug effects , Humans , Intracellular Signaling Peptides and Proteins/genetics , Lipopolysaccharides/pharmacology , Mice , Mitogen-Activated Protein Kinases/metabolism , NF-kappa B/metabolism , RNA Interference , RNA, Small Interfering/genetics , Receptors, Laminin/genetics , Toll-Like Receptor 4/genetics
3.
J Med Food ; 17(7): 742-8, 2014 Jul.
Article in English | MEDLINE | ID: mdl-24971771

ABSTRACT

The aim of this study was to clarify the efficacy of procyanidin C1 (Pro C1) for modulating vascular tone. Pro C1 induced a potent vasorelaxant effect on phenylephrine-constricted endothelium-intact thoracic aortic rings, but had no effect on denuded thoracic aortic rings. Moreover, Pro C1 caused a significant increase in nitric oxide (NO) production in endothelial cells. Pro C1-induced vasorelaxation and Pro C1-induced NO production were significantly decreased in the presence of a nonspecific potassium channel blocker (tetraethylammonium chloride [TEA]), an endothelial NO synthase inhibitor (N(G)-monomethyl-L-arginine [L-NMMA]), and a store-operated calcium entry inhibitor (2-aminoethyl diphenylborinate [2-APB]). Pro C1-induced vasorelaxation was also completely abolished by an inhibitor of soluble guanyl cyclase, which suggests that the Pro C1 effects observed involved cyclic guanosine monophosphate (cGMP) production. Interestingly, Pro C1 significantly enhanced basal cGMP levels. Taken together, these results indicate that Pro C1-induced vasorelaxation is associated with the activation of the calcium-dependent NO/cGMP pathway, involving potassium channel activation. Thus, Pro C1 may represent a novel and potentially therapeutically relevant compound for the treatment of cardiovascular diseases.


Subject(s)
Biflavonoids/pharmacology , Catechin/pharmacology , Cyclic GMP/metabolism , Nitric Oxide/metabolism , Proanthocyanidins/pharmacology , Signal Transduction/drug effects , Vasodilation/drug effects , Animals , Aorta, Thoracic/drug effects , Boron Compounds/pharmacology , Cells, Cultured , Endothelial Cells/drug effects , Guanylate Cyclase/antagonists & inhibitors , Guanylate Cyclase/metabolism , In Vitro Techniques , Male , Nitric Oxide Synthase Type III/metabolism , Potassium Channel Blockers/pharmacology , Rats , Rats, Sprague-Dawley , Tetraethylammonium/pharmacology , Vasodilator Agents/pharmacology , omega-N-Methylarginine/pharmacology
4.
Bioprocess Biosyst Eng ; 37(4): 707-10, 2014 Apr.
Article in English | MEDLINE | ID: mdl-23990129

ABSTRACT

In the conversion of lignocellulose into high-value products, including fuels and chemicals, the production of cellulase and the enzymatic hydrolysis for producing fermentable sugar are the largest contributors to the cost of production of the final products. The marine bacterium Saccharophagus degradans 2-40(T) can degrade more than ten different complex polysaccharides found in the ocean, including cellulose and xylan. Accordingly, S. degradans has been actively considered as a practical source of crude enzymes needed for the saccharification of lignocellulose to produce ethanol by others including a leading commercial company. However, the overall enzyme system of S. degradans for hydrolyzing cellulose and hemicellulose has not been quantitatively evaluated yet in comparison with commercial enzymes. In this study, the inductions and activities of cellulase and xylanase of cell-free lysate of S. degradans were investigated. The growth of S. degradans cells and the activities of cellulase and xylanase were promoted by adding 2 % of cellulose and xylan mixture (cellulose:xylan = 4:3 in mass ratio) to the aquarium salt medium supplemented with 0.2 % glucose. The specific cellulase activity of the cell-free lysate of S. degradans, as determined by the filter paper activity assay, was approximately 70 times lower than those of commercial cellulases, including Celluclast 1.5 L and Accellerase 1000. These results imply that significant improvement in the cellulase activity of S. degradans is needed for the industrial uses of S. degradans as the enzyme source.


Subject(s)
Alteromonadaceae/enzymology , Bacterial Proteins/chemistry , Cellulase/chemistry , Endo-1,4-beta Xylanases/chemistry , Lignin/chemistry
5.
FEBS Open Bio ; 3: 106-11, 2013.
Article in English | MEDLINE | ID: mdl-23847758

ABSTRACT

This study evaluated the effect of gamma irradiation on the reduction of the toxicity of mistletoe lectin using both in vitro and in vivo models. To extract the lectin from mistletoe, an (NH4)2SO4 precipitation method was employed and the precipitant purified using a Sepharose 4B column to obtain the pure lectin fraction. Purified lectin was then gamma-irradiated at doses of 0, 5, 10, 15, and 20 kGy, or heated at 100 °C for 30 min. Toxic effects of non-irradiated, irradiated, and heat-treated lectins were tested using hemagglutination assays, cytotoxicity assays, hepatotoxicity, and a mouse survival test and immunological response was tested using cytokine production activity. Hemagglutination of lectin was remarkably decreased (P < 0.05) by irradiation at doses exceeding 10 kGy and with heat treatment. However, lectin irradiated with 5 kGy maintained its hemagglutination activity. The cytotoxicity of lectin was decreased by irradiation at doses over 5 kGy and with heat treatment. In experiments using mouse model, glutamate oxaloacetate transaminase (GOT) and glutamic pyruvic transaminase (GPT) levels were decreased in the group treated with the 5 kGy irradiated and heat-treated lectins as compared to the intact lectin, and it was also shown that 5 kGy irradiated and heat-treated lectins did not cause damage in liver tissue or mortality. In the result of immunological response, tumor necrosis factor (TNF-α) and interleukin (IL-6) levels were significantly (P < 0.05) increased in the 5 kGy gamma-irradiated lectin treated group. These results indicate that 5 kGy irradiated lectin still maintained the immunological response with reduction of toxicity. Therefore, gamma-irradiation may be an effective method for reducing the toxicity of lectin maintaining the immune response.

6.
Eur J Pharmacol ; 714(1-3): 218-28, 2013 Aug 15.
Article in English | MEDLINE | ID: mdl-23770004

ABSTRACT

Numerous studies have shown various relationships between foods with a high nutritional value and a robust immune response, particularly studies that have focused on host protection and cytokine networks. This study aimed to clarify the role played by the procyanidin trimer C1 in innate and adaptive immunity. Procyanidin C1 did not exert cytotoxicity at concentrations ranging from 7.8 to 62.5 µg/ml in macrophage cells; therefore, concentration of 62.5 µg/ml was used as the maximum dose of procyanidin C1 throughout subsequent experiments. Procyanidin C1 enhanced inducible nitric oxide synthase-mediated nitric oxide production in a concentration-dependent manner. In addition, procyanidin C1 functionally induced macrophage activation by augmenting the expression of cell surface molecules (CD80, CD86, and MHC II) and proinflammatory cytokine production (tumor necrosis factor (TNF)-α, interleukin (IL)-1ß, and IL-6) via activation of mitogen-activated protein kinase (MAPK), e.g., p38, ERK, and JNK and nuclear factor (NF)-κB signaling pathways. Interestingly, procyanidin C1 effectively polarized T helper type 1 (Th1) by secreting Th1-mediated cytokines (interferon-γ, IL-12p70, and IL-2) and inducing splenocyte proliferation, indicating that procyanidin C1 contributes to Th1 polarization of the immune response. Accordingly, these findings confirms that the procyanidin C1 induces macrophage activation via NF-κB and MAPK pathways, leading to Th1 polarization in murine splenocytes, which suggests that procyanidin C1 regulates innate and adaptive immunity by macrophage activation and Th1 polarization.


Subject(s)
Biflavonoids/chemistry , Biflavonoids/pharmacology , Catechin/chemistry , Catechin/pharmacology , MAP Kinase Signaling System/drug effects , Macrophage Activation/drug effects , NF-kappa B/metabolism , Polymerization , Proanthocyanidins/chemistry , Proanthocyanidins/pharmacology , Spleen/cytology , Animals , Bone Marrow Cells/cytology , Cell Line , Cell Proliferation/drug effects , Cytokines/biosynthesis , Macrophages/cytology , Macrophages/drug effects , Macrophages/immunology , Macrophages/metabolism , Mice , Nitric Oxide/biosynthesis , Spleen/immunology , Th1 Cells/cytology , Th1 Cells/drug effects
7.
Biochem Biophys Res Commun ; 431(4): 698-705, 2013 Feb 22.
Article in English | MEDLINE | ID: mdl-23353651

ABSTRACT

Polyphenolic compounds have been regarded as one of the most promising dietary agents for the prevention and treatment of inflammation-related chronic diseases; however, the anti-inflammatory activities of flavonoids, such as quercetin, are not completely characterized, and many features remain to be elucidated. In this study, we showed the molecular basis for the downregulation of TLR4 signal transduction by quercetin. Quercetin markedly elevated the expression of the Toll-interacting protein, a negative regulator of TLR signaling. Lipopolysaccharide-induced expression of cell surface molecules (CD80, CD86, and MHC class I/II) and production of pro-inflammatory cytokines (tumor necrosis factor-α, IL-1ß, IL-6, and IL-12p70) were inhibited by quercetin, and this action was prevented by Toll-interacting protein silencing. In addition, quercetin-treated macrophages inhibited lipopolysaccharide-induced activation of mitogen-activated protein kinases, such as extracellular signal-regulated kinase 1/2, p38, and c-Jun N-terminal kinase, and the translocation of nuclear factor-κB and p65 through Toll-interacting protein. Treatment with quercetin resulted in a significant decrease in prostaglandin E2 and cyclooxygenase-2 levels as well as inducible nitric oxide synthase-mediated nitric oxide production induced by lipopolysaccharide. Taken together, these findings represent new insights into the understanding of negative regulatory mechanisms of the TLR4 signaling pathway and effective therapeutic intervention for the treatment of inflammatory disease.


Subject(s)
Anti-Inflammatory Agents, Non-Steroidal/pharmacology , Antineoplastic Agents/pharmacology , Intracellular Signaling Peptides and Proteins/biosynthesis , Quercetin/pharmacology , Toll-Like Receptor 4/antagonists & inhibitors , Animals , Cell Line , Down-Regulation , Intracellular Signaling Peptides and Proteins/genetics , Lipopolysaccharides , Mice , Mitogen-Activated Protein Kinase Kinases/metabolism , NF-kappa B/metabolism , RNA, Small Interfering/genetics , Signal Transduction/drug effects , Toll-Like Receptor 4/metabolism
8.
Int Immunopharmacol ; 15(2): 450-6, 2013 Feb.
Article in English | MEDLINE | ID: mdl-23261363

ABSTRACT

Natural products and dietary components rich in polyphenols have been shown to reduce inflammation; however, the molecular mechanisms underlying this anti-inflammatory activity are not completely characterized, and many features remain to be elucidated. This research was carried out to clarify the potential role of procyanidin trimer C1 in the anti-inflammatory effect of polyphenols. Procyanidin C1 inhibited inducible nitric oxide synthase-mediated nitric oxide production and the release of pro-inflammatory cytokines (interleukin-6 and tumor necrosis factor-α) in lipopolysaccharide (LPS)-induced macrophages. Treatment with procyanidin C1 resulted in a significant decrease in prostaglandin E2 and cyclooxygenase-2 levels, as well as the expression of cell surface molecules (CD80, CD86, and MHC class II), which was induced by LPS. Furthermore, our data demonstrated that the anti-inflammatory effect of procyanidin C1 occurs through inhibition of mitogen-activated protein kinase (p38 and c-Jun N-terminal kinase) and nuclear factor-κB signaling pathways. These 2 factors play a major role in controlling inflammation, through toll-like receptor 4, suggesting that procyanidin C1 plays a potent role in promoting anti-inflammatory activity in macrophages. These results represent a novel and effective therapeutic intervention for the treatment of inflammatory disease.


Subject(s)
Anti-Inflammatory Agents, Non-Steroidal/pharmacology , Biflavonoids/pharmacology , Catechin/pharmacology , Inflammation/drug therapy , Macrophages/drug effects , Proanthocyanidins/pharmacology , Toll-Like Receptor 4/immunology , Animals , Antigens, CD/metabolism , Cell Line , Cyclooxygenase 2/metabolism , Dinoprostone/metabolism , Lipopolysaccharides/immunology , MAP Kinase Signaling System/drug effects , MAP Kinase Signaling System/genetics , Macrophages/immunology , Mice , NF-kappa B/metabolism , Nitric Oxide/metabolism
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