Comparative analysis of the clinical outcomes between wavefront-guided and conventional femtosecond LASIK in myopia and myopia astigmatism.

AIM: To compare the clinical outcomes of wavefront guided femtosecond LASIK (WFG LASIK) and conventional femtosecond LASIK (NWFG LASIK) in eyes with myopia and myopia astigmatism. METHODS: This was a retrospective, nonrandomized, comparative investigation enrolling 236 eyes of 122 patients (18-50y) with low & moderate and high myopia. The WFG group including 97 eyes (50 patients) undergone WFG LASIK and the NWFG group including 139 eyes (72 patients) undergone conventional LASIK. Mean efficacy index, high order aberrations (HOAs), pupil size and the quality of visual questionnaire were evaluated 6mo postoperatively. RESULTS: There is no difference between WFG group (-0.054±0.049 in logMAR) and NWFG group (-0.040±0.056) in uncorrected distance visual acuity (UDVA) postoperatively. The myopia astigmatism is higher in WFG group than that in NWFG group (P<0.05). However, the mean efficacy index (MEI) in the WFG group (1.09±0.106) is better than that in the NWFG group (1.036±0.124; P<0.001). Increased HOAs were observed in NWFG group (0.30±0.196) than that in WFG group (0.146±0.188; P<0.001). The pupil size is larger in WFG group (5.15±0.76 mm) than that in NWFG group (4.32±0.52 mm). The patients are satisfied with the clinical surgery, yet WFG group showed better visual quality using the questionnaire survey. Meanwhile, high myopia would result in worse MEI, HOAs and visual quality than low & moderate myopia. CONCLUSION: WFG and NWFG FS-LASIK are both effective and safe procedures to correct low & moderate and high myopia, but WFG FS-LASIK gives a better postoperative MEI, aberrometric control and predictable outcome. Meanwhile, WFG FS-LASIK is better than NWFG FS-LASIK in correction of myopia astigmatism. Low & moderate myopia allow better clinical outcomes than high myopia using any surgical method.

EDIL3 depletion suppress epithelial-mesenchymal transition of lens epithelial cells via transforming growth factor ß pathway.

AIM: To study the effect of discoidin I-like domaincontaining protein 3 (EDIL3) depletion on the proliferation and epithelial-mesenchymal transition (EMT) in human lens epithelial cells (LECs). METHODS: RNA interference was used to inhibit the expression of EDIL3 in human LECs in vitro. The morphology of cells was observed using an inverted microscope. Cell proliferation was assessed using EdU kit. Cell migration was investigated using Transwell chamber and EMT of LECs was assessed using confocal microscope and Western blotting. The transforming growth factor ß (TGFß) pathway was investigated using Western blotting. RESULTS: The data showed that silencing EDIL3 expression changed LECs morphology and suppressed LECs proliferation (P<0.05) and migration (P<0.01). Furthermore, the result of Western blotting showed that EDIL3 depletion reduced the expression of α-smooth muscle actin (α-SMA) (P<0.001) and vimentin (P<0.01), while increased the expression of E-cadherin (P<0.001). EDIL3 depletion could suppress the phosphorylation of Smad2 (P<0.01) and Smad3 (P<0.01) and the activation of exracellular signal regulated kinase (ERK) (P<0.05). CONCLUSION: The findings indicate that EDIL3 might participate in the proliferation and EMT in LECs via TGFß pathway and may be a potential therapeutic target for the treatment of posterior capsule opacification.

[Extraction and characterization of total DNA from Dendrobium].

OBJECTIVE: To explore the quality of DNA with three methods of DNA extraction and the influence on RAPD-PCR. METHOD: the electrophoresis of total DNA, UV spectrophotometry and RAPD analysis were carried out on DNA extracted from three different methods. RESULT: The DNA concentration and yields were different, which greatly influenced the result of RAPD-PCR. CONCLUSION: The higher quality DNA from Dendrobium can be obtained with the method of CTAB-free extraction medium before total DNA was isolated.

[Cloning a gene related to resistance to TuMV in cabbage].

A cDNA library was constructed from cabbage 84075 which resists TuMV. The degenerate primers was designed with the disease resistance gene conservative domain (NBS-LRR). The fragments of 513 bp were amplified by RT-PCR and genomic DNA PCR from resistant material 84075, then cloned and sequenced. Two recombinants which are highly homologous with the resistance genes cloned in other plants were used as probes, (named as Bor1, Bor2), the cDNA library was screened. A positive clone was obtained, named as TuR2, whose length was 762 bp, which encodes 226 amino acids, contains a long 681 bp open reading frame (ORF), and has different homology score of amino acid compared with the cloned resistance disease genes of other plants. TuR2 was used as probe. Southern blotting hybridization with genomic DNA shows that TuR2 is probably present in single copy; No. rthern blotting hybridization with RNA shows that the gene expression is constitutive and not differential in every part of the resistance plant 84075, the result of separating detection in F2 population shows that TuR2 gene is probably related to resistance to TuMV in cabbage.