ABSTRACT
Two new dammarane-type triterpenoids, dammar-3α,12(R),20(S)-triol-12,32(R);20,32-diepoxy-25-methy-25-en-tridecacyclic ether (1) and (23E)-12ß,20(R),25(S),26-tetrahydroxydammar-23-en-3-one (2) were isolated from the green walnut husks of Juglans mandshurica Maxim together with six known compounds. Their structures were elucidated through extensive spectroscopic analyses and by comparison with the literature, and the cytotoxic activities of these compounds were evaluated.
Subject(s)
Antineoplastic Agents, Phytogenic , Juglans , Triterpenes , Antineoplastic Agents, Phytogenic/chemistry , Juglans/chemistry , Plant Extracts/chemistry , Triterpenes/pharmacology , DammaranesABSTRACT
Five new α-tetralone glycosides, juglanbiosides A-E (1-5), together with an α-tetralone derivative (15) and nine known 1,4-naphthoquinones (6-14) were isolated from the 95% EtOH extract of green walnut husks of Juglans mandshurica Maxim. Their structures were elucidated by comprehensive spectroscopic methods (1H, 13C NMR, DEPT, HSQC, HMBC, CD, HR-ESI-MS). In vitro cytotoxicities of all the isolated compounds were evaluated against BGC-823, HCT-15 and K562 cancer cell lines.[Formula: see text].
Subject(s)
Antineoplastic Agents, Phytogenic/isolation & purification , Glycosides/pharmacology , Juglans/chemistry , Nuts/chemistry , Antineoplastic Agents, Phytogenic/chemistry , Antineoplastic Agents, Phytogenic/pharmacology , Cell Line, Tumor , Glycosides/chemistry , Glycosides/isolation & purification , Humans , Molecular Structure , Naphthoquinones/chemistry , Naphthoquinones/isolation & purification , Naphthoquinones/pharmacology , Plant Extracts/chemistry , Spectrum Analysis , Tetralones/chemistry , Tetralones/isolation & purification , Tetralones/pharmacologyABSTRACT
A new triterpene named klodorol B (1), together with six known compounds, were isolated from the green walnut husks of Juglans mandshurica Maxim. Their structures were determined using spectroscopic methods on the basis of 1D and 2D NMR, and high-resolution electrospray ionization mass spectrometry. The isolated compounds were evaluated for their cytotoxic activities on human gastric carcinoma (BGC-823), human liver cancer (HepG-2) and human lung cancer (A549) cell lines. .
Subject(s)
Antineoplastic Agents, Phytogenic/pharmacology , Juglans/chemistry , Liver Neoplasms/drug therapy , Lung Neoplasms/drug therapy , Plant Extracts/pharmacology , Stomach Neoplasms/drug therapy , Triterpenes/pharmacology , A549 Cells , Cell Line, Tumor , Hep G2 Cells , Humans , Magnetic Resonance Spectroscopy , Nuts/chemistry , Triterpenes/analysisABSTRACT
Two new tetralone glycosides, 4(S)-5-methoxy juglanoside A (1), 4(S)-5-methoxy juglanoside D (2), together with ten known compounds (3-12) have been isolated from the green walnut husks of Juglans mandshurica Maxim. Their structures were elucidated on the basis of their ESI-MS, 1 D and 2 D NMR spectroscopic data. In addition, all compounds were evaluated for their cytotoxic activities against the cancer BGC-823 (human gastric carcinoma), HCT-15 (human colorectal carcinoma) and K562 (human chronic myeloid leukemia) cell lines. The results showed aglycones of naphthoquinones had stronger cytotoxic activities than glycosides of tetralone.
Subject(s)
Early Detection of Cancer , Glycosides/isolation & purification , Juglans/chemistry , Antineoplastic Agents, Phytogenic/chemistry , Cell Line, Tumor , Glycosides/chemistry , Glycosides/pharmacology , Humans , Molecular Structure , Naphthoquinones/pharmacology , Nuts/chemistry , Plant Extracts/chemistry , Spectrum Analysis , Tetralones/chemistry , Tetralones/isolation & purification , Tetralones/pharmacologyABSTRACT
AIM: To investigate the proteome composition and function of human neonatal arterial umbilical cord. METHODS: Serum proteomic analyses were performed on samples from both males and females by using a combination of techniques: (1) removal of six high-abundance proteins, (2) tryptic digestion of low-abundance proteins, (3) separation of peptide mixtures by reverse-phase high-performance liquid chromatography (RP-HPLC), and (4) peptide identification using electrospray ionization tandem mass spectrometry (ESI-MS/MS). RESULTS: A total of 837 non-redundant proteins were identified, with 213 male-specific and 239 female-specific proteins. Among them, 319 proteins were identified by at least 2 distinct peptides. The subcellular localization, function, and pathway involvement for each of the identified proteins were analyzed. A comparison of this neonatal proteome to that of adult serum proteome revealed novel biomarkers, such as alpha-fetoprotein and periostin that were specific to newborn infants. CONCLUSION: These data will contribute to a better understanding of the composition of umbilical cord serum and aid the discovery of novel biomarkers for the prenatal diagnosis of fetal abnormalities.
