Identification of two terpenoids that accumulate in Chinese water chestnut in response to fresh-cut processing.

As a form of vegetable in China, freshly cut corms of Chinese water chestnuts (Eleocharis dulcis) are well received by consumers. Few studies have investigated the metabolites present in fresh-cut E. dulcis, particularly during the storage stage. Two compounds, triterpenoids and apocarotenoids, were identified in fresh-cut E. dulcis during the late storage period using thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC), and nuclear magnetic resonance (NMR) spectroscopy. The content of these two compounds gradually increased in the surface tissue of fresh-cut E. dulcis during storage. Moreover, the transcript levels of 10 genes involved in terpenoid backbone biosynthesis and five genes involved in carotenoid precursor biosynthesis were evaluated via quantitative real-time PCR (qRT-PCR). Expression of the rate-limiting enzyme-coding genes CwDXS and CwHMGS was significantly induced by wounding. CwMYC and CwbHLH18, which belong to bHLH transcription factors (TFs) IIIe and VIa subgroup, were isolated from E. dulcis corm. Phylogenetic analysis showed that CwMYC and CwbHLH18 grouped with other terpenoid-regulated bHLHs, and their transcript levels were strongly induced after fresh-cut processing. These results suggested that the biosynthesis of terpenoids and apocarotenoids in fresh-cut E. dulcis strongly depended on the transcriptional regulation of structural genes involved in the methylerythritol 4-phosphate (MEP) and mevalonate (MVA) pathways. However, the complex secondary metabolism of fresh-cut E. dulcis during late storage requires further investigation.

Methyl jasmonate treatment alleviates chilling injury and improves antioxidant system of okra pod during cold storage.

Okra pod is sensitive to low temperature, which results in chilling injury under improper low-temperature storage. This study aimed to evaluate the effect of different concentrations of methyl jasmonate (MeJA) treatment on okra pod stored at 4 ± 1°C for 12 days and illuminate the mechanism of MeJA alleviating chilling injury. Compared to the control, MeJA treatments maintained lower relative electric conductivity (REC), chilling injury (CI) degree, and lignin content, as well as higher total soluble solids, total soluble sugar, pectin content, and chlorophyll content. The factor analysis was applied to comprehensively evaluate the effects of MeJA so that 1 µmol/L MeJA was screened as the optimum concentration to maintain the okra quality throughout the storage time. In contrast with control, MeJA not only accelerated the generation of antioxidant substances (phenolics and flavonoids) but also increased the superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), and peroxidase (POD) activity, inhibited malondialdehyde (MDA), hydrogen peroxide (H2O2) content accumulation, and the polyphenol oxidase (PPO) activity. This work confirmed that MeJA could effectively alleviate chilling injury and maintain the quality during cold-stored by regulating reactive oxygen species (ROS) metabolism. These results provide theoretical guidance for the application of MeJA in okra storage and preservation.

Taste Compound Generation and Variation in Chinese Water Chestnut (Eleocharis dulcis (Burm.f.) Trin. ex Hensch.) Processed with Different Methods by UPLC-MS/MS and Electronic Tongue System.

Chinese water chestnut (CWC) is popular among consumers due to its unique flavor and crisp and sweet taste. Thus far, the key substances affecting the taste compound of CWC are still unclear. In this study, we used UPLC-MS/MS and an electronic tongue system to study the effects of four typical steaming and cooking methods, cooking without peel for 10 min (PC), steaming without peel for 15 min (PS), cooking with peel for 30 min (WPC), steaming with peel for 30 min (WPS), on the taste compound generation and variation of CWC, and revealed the secret of its crisp and sweet taste. The results show that the electronic tongue can effectively identify the taste profile of CWC, and the effective tastes of CWC were umami, bitterness, saltiness, and sweetness. We screened 371 differential compounds from 640 metabolic species. Among them, nucleotides and their derivatives, carbohydrates, organic acids and their derivatives, and amino acids and their derivatives are closely related to the key taste of CWC, and these compounds affected the taste of CWC through six related metabolic pathways: oxidative phosphorylation and purine metabolism; alanine, aspartate, and glutamate; bile secretion; amino sugar and nucleotide sugar metabolism; the phenylpropane pathway; and toluene degradation. This study reveals the potential metabolic causes of taste compound generation and variation in the taste of CWC.

Comparison of flavonoids and phenylpropanoids compounds in Chinese water chestnut processed with different methods.

Different processing methods of Chinese water chestnut (CWC; Eleocharis dulcis (Burm.f.) Trin. ex Hensch.) steaming with skin (WPC), cooking with skin (WPS), steaming with peeling (PS), fresh cutting (FF) and cooking with peeling (PC) were compared. Liquid chromatography-mass spectrometry was used to analyze the metabolic profiles of the processed samples. A total of 454 metabolites, including 123 flavonoids and 57 phenylpropanoids, were characterized. The flavonoid and phenylpropanoid profiles were distinguished using PCA. Eighteen flavonoids and six phenylpropanoids were detected and quantitated in the WPC and WPS samples but not in the FF, PC and PS samples. In addition to the O-hexoside of tricin, kaempferol and luteolin were the predominant flavonoids in the WPC and WPS samples, and all three compounds were higher in the WPC and WPS samples than in the FF sample. This study provides new results regarding differences in the metabolite profile of CWC processed with different methods.

Drying kinetics, antioxidants, and physicochemical properties of litchi fruits by ultrasound-assisted hot air-drying.

To obtain better qualities of litchi fruits, fruit pulps were subjected to ultrasonic treatment (UT) followed by drying. Samples were subjected to UT at 3 W/g for 10 min with distilled or ice water and compared with non-UT dried samples. After drying, vitamin C, total phenolic content, color, texture, nutrition, microbial load, drying kinetics, and shelf life were assessed. Results suggest that shear stress plus increasing heat reduced drying time by about 50%, and retained 70% vitamin C and 60% total phenolic content. UT led to about 75% of vitamin C and 70% total phenolic content through inhibition of ultrasonic heat. No significant differences were found in redness, yellowness, and hardness. Inhibition of ultrasound heat resulted in about 27% glucose, 22% fructose, 17% sucrose, and prolonged storage time. Inhibition of increasing ultrasound heat allows low drying cost and high product quality of litchi fruit in air-drying. PRACTICAL APPLICATIONS: UT promotes drying efficiency and preserves product quality. However, this treatment triggers the loss of antioxidants and sugars of litchi fruits when water temperature arises in the treatment. Additional use of ice crystals can offset the thermal effect of the UT; this mechanism reduces the diffusion and loss of nutrients from the material to the solution. This strategy is simple and feasible to improve the drying rate and to retain the content of antioxidants, and further improve the flavor and storage quality of dried litchi fruits.