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1.
Anim Sci J ; 87(2): 202-8, 2016 Feb.
Article in English | MEDLINE | ID: mdl-26278910

ABSTRACT

Six growing lambs were used to evaluate the feeding value of two forage-based diets in a long-term feeding period by measuring body weight (BW) gain, digestibility, nitrogen (N) retention and microbial N (MBN) yield. The animals were fed imported low-quality timothy hay (TH) with concentrate diet (THD) or imported low-quality Italian ryegrass straw (IR) with concentrate diet (IRD) for 9 months. The forages were offered at 2% BW, and concentrate was fed at 40% of forage intake. The BW gain averaged 82.6 and 66.2 g/day for THD and IRD, respectively, without showing significant difference. Average forage intake (% BW) was significantly greater for IR than for TH, although it was not affected by feeding periods. The digestibility did not differ between diets or periods. The numerically greater (P = 0.06) ratio of retained N to absorbed N for IRD than that for THD was prominent. Neither diet nor period had significant effect on MBN supply and efficiency of MBN synthesis. The results suggest that the IR-based diet can be also used for long-term periods of feeding to growing ruminant animals as a grass hay-based diet without any detrimental effects on nutrient utilization and growth performance.


Subject(s)
Animal Feed , Animal Nutritional Physiological Phenomena/physiology , Lolium , Nitrogen/metabolism , Phleum , Sheep/growth & development , Sheep/physiology , Animals , Digestion/physiology , Male , Weight Gain
2.
Anim Sci J ; 86(11): 943-51, 2015 Nov.
Article in English | MEDLINE | ID: mdl-26153850

ABSTRACT

The objective of the present study was to conduct an adipogenic evaluation of different roughage sources and feeding levels during ruminant adipocyte differentiation in vitro. Six wether sheep were divided into a timothy hay feeding group (TFG, n = 3) and an Italian ryegrass straw feeding group (IFG, n = 3). The sheep were fed high-roughage (HR), medium roughage (MR) and low-roughage (LR) diets in a one-way layout design each over a 6-day period. Sheep serum samples collected on the last day of each dietary treatment were added to an adipogenic induction medium for differentiation of preadipocytes derived from sheep subcutaneous adipose tissue. The cytoplasmic lipid accumulations in the TFG serum-treated preadipocytes were significantly higher than those of the IFG-serum treated preadipocytes on day 12. Messenger RNA expression of CCAAT/enhancer-binding protein (C/EBP)-α, C/EBP-ß, C/EBP-δ, fatty-acid-binding protein (aP2) and stearoyl-coenzyme A desaturase (SCD) were regulated by each serum treatment. This study shows that different roughage source diets and roughage-to-concentrate ratio diets can regulate adipocyte differentiation via ruminant blood composition.


Subject(s)
Adipocytes/cytology , Adipocytes/metabolism , Adipogenesis , Animal Feed , Dietary Fiber , Sheep/metabolism , Adipose Tissue/cytology , Animals , Cell Differentiation , Cells, Cultured , Culture Media , Cytoplasm/metabolism , Lipid Metabolism , Male , Subcutaneous Tissue
3.
Anim Sci J ; 83(12): 788-95, 2012 Dec.
Article in English | MEDLINE | ID: mdl-23216544

ABSTRACT

Although our previous report demonstrated that adiponectin and AdipoR1 gene expressions changed among different lactation stages in the bovine mammary gland, its in vivo kinetics remain unclear in ruminant animals. In this study, we investigated the changes in circulating concentrations of adiponectin, as well as other metabolic hormones and metabolites, (i) during the periparturient period and (ii) among different lactation stages, in Holstein dairy cows. In experiment 1, serum adiponectin concentrations increased after parturition. Serum insulin concentrations were lower in the postpartum than prepartum period, whereas serum growth hormone (GH) concentrations increased in the postpartum period. Serum nonesterified fatty acids (NEFA) levels were increased during the postpartum period and were dependent on the parity. In experiment 2, there was no significant difference in plasma adiponectin concentrations among lactational stages. Plasma insulin concentrations tended to be lower in early lactation while plasma GH levels tended to be higher. Plasma NEFA concentrations were significantly lower in mid- and late-lactation stages than non-lactation stages. These findings indicate that elevation of serum adiponectin might be involved in energy metabolism just around parturition, and might exert its action through regulation of receptor expression levels in target tissues in each lactational stage in Holstein dairy cows.


Subject(s)
Adiponectin/blood , Cattle/physiology , Energy Metabolism/physiology , Lactation/physiology , Parturition/physiology , Animals , Fatty Acids, Nonesterified/blood , Female , Growth Hormone/blood , Insulin/blood , Lactation/blood , Parturition/blood
4.
Anim Sci J ; 83(3): 263-7, 2012 Mar.
Article in English | MEDLINE | ID: mdl-22435632

ABSTRACT

Accumulating data suggest a relationship between chemerin and energy metabolism. Our group previously described gene cloning, expression analysis and the regulatory mechanism of chemerin and its own receptor in mice and cattle. The objective of the present study was to investigate the physiological effect of chemerin on endocrine changes and energy metabolism in sheep using a biologically stable chemerin analog. The chemerin analog was intravenously administrated (100 or 500 µg/head) to sheep, and plasma insulin and metabolites (glucose, nonesterified fatty acids (NEFA), triglyceride, total cholesterol and high-density lipoprotein (HDL) cholesterol) were analyzed. The chemerin analog dramatically increased the insulin levels, and glucose levels were decreased. NEFA levels were slightly decreased at 20 min but then increased gradually from 60 to 180 min after analog administration. In addition, injection of the chemerin analog immediately increased triglyceride and total cholesterol but not HDL levels. These results suggested that chemerin analog regulated insulin secretion related to glucose metabolism and the release of triglycerides in sheep in vivo. This study provides new information about endocrine and metabolic changes in response to chemerin in sheep.


Subject(s)
Chemokines/physiology , Energy Metabolism/physiology , Sheep/metabolism , Animals , Blood Glucose/metabolism , Cholesterol/blood , Fatty Acids, Nonesterified/blood , Insulin/metabolism , Insulin Secretion , Lipoproteins, HDL/blood , Triglycerides/blood
5.
Anim Sci J ; 82(1): 99-106, 2011 Feb.
Article in English | MEDLINE | ID: mdl-21269367

ABSTRACT

Although the functions of adiponectin, a differentiated adipocyte-derived hormone, in regulating glucose and fatty acid metabolism are regulated by two subtypes of adiponectin receptors (AdipoRs; AdipoR1 and AdipoR2), those in ruminants remain unclear. Therefore we examined the messenger RNA (mRNA) expression levels of adiponectin and its receptors in various bovine tissues and mammary glands among different lactation stages, and the effects of lactogenic hormones (insulin, dexamethasone and prolactin) and growth hormone (GH) on mRNA expression of the AdipoRs in cultured bovine mammary epithelial cells (BMEC). AdipoRs mRNAs were widely expressed in various bovine tissues, but adiponectin mRNA expression was significantly higher in adipose tissue than in other tissues. In the mammary gland, although adiponectin mRNA expression was significantly decreased at lactation, AdipoR1 mRNA expression was significantly higher at peak lactation than at the dry-off stage. In BMEC, lactogenic hormones and GH upregulated AdipoR2 mRNA expression but did not change that of AdipoR1. In conclusion, adiponectin and its receptor mRNA were expressed in various bovine tissues and the adiponectin mRNA level was decreased during lactation. These results suggest that adiponectin and its receptors ware changed in mammary glands by lactation and that AdipoRs mRNA expression was regulated by different pathways in BMEC.


Subject(s)
Adiponectin/genetics , Adiponectin/metabolism , Epithelial Cells/metabolism , Gene Expression Regulation , Growth Hormone/pharmacology , Mammary Glands, Human/cytology , Mammary Glands, Human/metabolism , Prolactin/pharmacology , Animals , Cattle , Cells, Cultured , Dexamethasone/pharmacology , Female , Growth Hormone/physiology , Humans , Insulin/pharmacology , Insulin/physiology , Lactation/genetics , Lactation/physiology , Prolactin/physiology , RNA, Messenger/metabolism , Receptors, Adiponectin/genetics , Receptors, Adiponectin/metabolism , Theophylline/analogs & derivatives , Up-Regulation
6.
Tohoku J Exp Med ; 219(4): 311-7, 2009 Dec.
Article in English | MEDLINE | ID: mdl-19966531

ABSTRACT

Adipocyte differentiation is an important aspect in energy homeostasis. Although the regulation of adipocyte differentiation is relatively well understood, the underlying molecular mechanism remains unclear. In this study, subcutaneous and epididymal adipose tissues were used to study the differential expression of associated genes. We found that the expression level of mouse homologue of rat prostatic androgen-repressed message-1 (mPARM-1) gene was higher in subcutaneous, perirenal and mesenteric adipose tissues than in epididymal adipose tissue. In mouse subcutaneous, perirenal, and mesenteric adipose tissues, the expression level of this gene was higher in adipocytes than in non-adipocyte cells, i.e. stromal-vascular cells. Furthermore, mPARM-1 mRNA expression was up-regulated in subcutaneous, mesenteric, and epididymal adipose tissues of mice fed a high-fat diet compared to those fed a normal-fat diet. Expression level of mPARM-1 mRNA increased in the early stage of the chemically induced adipocyte differentiation, preceding the increase in peroxisome proliferator-activated receptor-gamma 2 (PPAR-gamma2) mRNA. Tumor necrosis factor-alpha (TNF-alpha), an inhibitor of adipocyte differentiation, reduced the expression of mPARM-1 mRNA in differentiated 3T3-L1 cells and subsequently down-regulated the expression of adipogenic genes, including PPAR-gamma2, leptin and adipogenin. Moreover, knockdown of mPARM-1 expression with siRNA reduced lipid accumulation and the expression levels of PPAR-gamma2 and adipocyte protein 2 mRNAs, which suggest that the degree of adipocyte differentiation of 3T3-L1 cells has been reduced. These results indicate that mPARM-1 might be involved in the regulation of fat accumulation and adipocyte differentiation.


Subject(s)
Adipocytes/cytology , Adipocytes/metabolism , Androgen-Binding Protein/metabolism , Cell Differentiation , 3T3-L1 Cells , Adipocytes/drug effects , Androgen-Binding Protein/genetics , Animals , Cell Differentiation/drug effects , Dietary Fats/administration & dosage , Dietary Fats/pharmacology , Gene Expression Profiling , Gene Expression Regulation/drug effects , Male , Mice , Mice, Inbred C57BL , Molecular Sequence Data , RNA, Messenger/genetics , RNA, Messenger/metabolism , RNA, Small Interfering/metabolism , Sequence Analysis, DNA , Transfection , Tumor Necrosis Factor-alpha/pharmacology
7.
Biochem Biophys Res Commun ; 362(4): 1013-8, 2007 Nov 03.
Article in English | MEDLINE | ID: mdl-17767914

ABSTRACT

Chemerin, an 18 kDa protein secreted by adipose tissue, was reported to modulate immune system function through its binding to the chemerin receptor (chemerinR). We herein demonstrate that chemerin also influences adipose cell function. Our data showed that chemerin and chemerinR mRNA expressions were highly expressed in adipose tissues, and that their expression levels were up-regulated in mice fed a high-fat diet. Both chemerin and chemerinR mRNA expression dramatically increased during the differentiation of 3T3-L1 cells and human preadipocytes into adipocytes. Furthermore, recombinant chemerin induced the phosphorylation of extracellular signal-regulated kinases 1/2 (ERK 1/2) and lipolysis in differentiated 3T3-L1 adipocytes. Thus, the adipokine chemerin likely regulates adipocyte function by autocrine/paracrine mechanisms.


Subject(s)
Adipocytes/cytology , Adipocytes/physiology , Adipogenesis/physiology , Chemotactic Factors/metabolism , Intercellular Signaling Peptides and Proteins/metabolism , Receptors, Chemokine/metabolism , 3T3 Cells , Adaptation, Physiological , Animals , Cell Differentiation , Cells, Cultured , Chemokines , Mice , Mice, Inbred C57BL
8.
Biochem Biophys Res Commun ; 354(2): 591-7, 2007 Mar 09.
Article in English | MEDLINE | ID: mdl-17250804

ABSTRACT

Recently, it has been found that long-chain fatty acids activate the G protein-coupled receptors (GPRs), GPR120 and GPR40. However, there have been no reports to date on the possible physiological roles of these GPRs in adipose tissue development and adipocyte differentiation. GPR120 mRNA was highly expressed in the four different adipose tissues, and the amount of mRNA was elevated in adipose tissues of mice fed a high fat diet. However, GPR40 mRNA was not detected in any of the adipose tissues. The expression of GPR120 mRNA was higher in adipocytes compared to stromal-vascular (S-V) cells. The level of GPR120 mRNA increased during adipocyte differentiation in 3T3-L1 cells. Similar results were observed in human adipose tissue, human preadipocytes, and cultured adipocytes. Moreover, use of a small interference RNA (siRNA) to down-regulate GPR120 expression resulted in inhibition of adipocyte differentiation. Our results suggest that GPR120 regulates adipogenic processes such as adipocyte development and differentiation.


Subject(s)
Adipocytes/cytology , Adipogenesis/physiology , Receptors, G-Protein-Coupled/physiology , 3T3-L1 Cells , Animals , Female , Mice , Mice, Inbred C57BL
9.
Biosci Biotechnol Biochem ; 69(11): 2117-21, 2005 Nov.
Article in English | MEDLINE | ID: mdl-16306693

ABSTRACT

To investigate the role of claudin-6 in adipogenesis, claudin-6 mRNA was examined in adipose tissues and adipocyte differentiation. Claudin-6 mRNA was found to be differentially expressed in four different adipose tissues, and up-regulated in each fat depot of mice fed a high-fat diet as compared to a normal-fat diet. Levels of claudin-6 transcripts were increased during differentiation of 3T3-L1 cells in vitro. Moreover, small interfering RNA (siRNA)-mediated reduction of claudin-6 mRNA inhibited differentiation of 3T3-L1 cells. These results suggest that claudin-6 is another important regulator in adipogenesis and fat deposition.


Subject(s)
Adipogenesis/genetics , Membrane Proteins/genetics , Up-Regulation/genetics , 3T3-L1 Cells , Adipose Tissue/chemistry , Adipose Tissue/cytology , Animals , Cell Differentiation/drug effects , Claudins , Dietary Fats/pharmacology , Male , Mice , Mice, Inbred C57BL , RNA, Messenger/analysis , RNA, Small Interfering/pharmacology
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