[Extraction of triterpenoids from fruiting [corrected] bodies of Ganoderma lucidum by supercritical fluid extraction].

OBJECTIVE: To optimize the extracting condition for triterpenoids from the fruits of Ganoderma lucidum by supercritical fluid extraction (SFE). METHOD: Optimum extraction conditions were studied by orthogonal tests. The total triterpenoids were determined by ultraviolet spectrophotometry and ganoderic acid B was determined by RP-HPLC. RESULT: The optimal extraction conditions were that the pressure 25 MPa, the temperature was 45 degrees C, the extraction time was 1.5 hour, and the ethanol was employed as modifier carrier at the volume of 3 mL x g(-1). CONCLUSION: SFE is superior and it is feasible to extract triterpenoids from fruits of Ganoderma lucidum.