ABSTRACT
OBJECTIVE: To study the protein changes in the prostate fluid with abnormal lecithin bodies, and explore their relationship with the pathogenesis of chronic prostatitis. METHODS: Using the tandem mass tag (TMT) technology, we identified differential proteins in the prostate fluid with abnormal lecithin bodies in normal males and analyzed their biological functions by GO functional annotation and KEGG pathways. RESULTS: Totally, 377 differential proteins were identified, 238 up-regulated and the other 139 down-regulated, and 8 of the top 20 significant differential proteins were related to inflammation and immune regulation. In the top 30 of the 1 011 items enriched by GO annotation, 5 were related to immune function and 3 to inflammatory response, and of the 251 KEGG pathways, 9 were involved in inflammation and immune regulation. CONCLUSIONS: Changes in lecithin bodies lead to proteomic changes in the prostatic fluid, which is closely related to protein- and pathway-associated inflammatory response and immune disorders, as well as to the immunologic mechanism of chronic prostatitis patients, and therefore can be used as an indicator of chronic prostatitis.
Subject(s)
Lecithins , Prostatitis , Chronic Disease , Humans , Male , Prostate , ProteomicsABSTRACT
A field mesocosm experiment with Chinese fir (Cunninghamia lanceolata) seedlings was conducted in Chenda State-Owned Forest Farm, Sanming, Fujian Province. The effects of soil warming (ambient +5 â) on specific respiration rates and nonstructural carbohydrate (NSC) concentrations in fine roots were measured by the ingrowth core method, to reveal the belowground responses and the adaptability of Chinese fir to global warming. The results showed that soil warming caused significant changes of fine root NSC in the second year. The NSC and starch concentrations in 0-1 mm fine roots, and the NSC and sugar concentrations in 1-2 mm fine roots decreased signifi-cantly in January. The NSC, sugar and starch concentrations in 0-1 mm roots and the starch concentration in 1-2 mm roots increased in July. Soil warming had no significant effect on fine root NSC in the third year. The specific root respiration rate of the 0-1 mm roots significantly increased in July of the second year but significantly decreased in July of the third year in the warmed plots. Compared with the 0-1 mm roots, soil warming had no significant effect on the specific root respiration rate of the 1-2 mm roots. In conclusion, the responses of fine root respiration to soil warming depended on the duration of warming. Fine root respiration partly acclimated to soil warming with increasing duration of soil warming, which kept fine root NSC being relatively stable.
