ABSTRACT
OBJECTIVE: To investigate the genetic diversity and genetic relationship of different forms of Trichosanthes kirilowii from Shandong Province. METHODS: Different forms of Trichosanthes kirilowii from the same planting base were detected by random amplified polymorphic DNA( RAPD) with eight random primers. The amplified bands were detected and the fingerprint was drawn by Biosens Gel Imaging System software. The Jaccard coefficient was worked out by using NTSYS-pc software, and a cluster dendrogram of different samples was established based on unweighted pair-group method with arithmetic means (UPGMA). RESULTS: The eight primers produced 56 bands, among which, 32 bands were polymorphic. The cluster dendrogram displayed there was some differences of genetic relationship of different samples. CONCLUSION: There is similar genetic material basis of Trichosanthes kirilowii from the same planting base, but also there is apparent individual differences.
