ABSTRACT
Scutellaria barbata D. Don (Lamiaceae)(SB) is a perennial herb which is natively distributed throughout Korea and southern China. This herb is known in traditional Chinese Medicine as Ban-Zhi-Lian and traditional Korean medicine as Banjiryun, respectively. SB has been used as an anti-inflammatory and antitumor agent. We aimed to determine the expressin of cell cycle-related signal molecules for growth inhibition after HCG treatment by the herb SB in two different human myometrial smooth muscle cells (SMCs) and leiomyomal SMCs. Water-soluble ingredients of SB, myometrial SMCs and the leiomyomal cell lines were used in vitro. Uterine myomas often enlarge rapidly during pregnancy, implying that human chorionic gonadotrophin (HCG) may influences cell proliferation in uterine leiomyomata. We investigated the effects of SB on the cell proliferation and the expression of cell cycle-related proteins in these cells. Although HCG/LH receptor was present in both cultured myometrial and leiomyomal cells, as assayed by reverse transcription polymerase chain reaction analysis, treatment with HCG significantly increased cell proliferation in both myometrial and leiomyomal cells. However, SB reduced the proliferative effect of HCG in leiomyoma and myometrial cells, respectively. In HCG-treated leiomyomal cells, the expression of proliferating cell nuclear antigen, cyclin E and cdc2 was significantly reduced by SB treatment. These results suggest that SB reduced the HCG-promoted proliferation of myometrial and leiomyomal cells.
Subject(s)
Cell Cycle Proteins/metabolism , Chorionic Gonadotropin/antagonists & inhibitors , Drugs, Chinese Herbal/pharmacology , Leiomyoma , Myocytes, Smooth Muscle/drug effects , Myometrium/drug effects , Scutellaria , Uterine Neoplasms , Actins/genetics , Actins/metabolism , Adult , CDC2 Protein Kinase/genetics , CDC2 Protein Kinase/metabolism , CDC2-CDC28 Kinases/genetics , CDC2-CDC28 Kinases/metabolism , Cell Cycle Proteins/biosynthesis , Cell Proliferation/drug effects , Cell Survival/drug effects , Cells, Cultured , Cyclin E/genetics , Cyclin E/metabolism , Cyclin-Dependent Kinase 2 , Female , Gene Expression Regulation, Neoplastic/drug effects , Humans , Myocytes, Smooth Muscle/metabolism , Myometrium/metabolism , Proliferating Cell Nuclear Antigen/genetics , Proliferating Cell Nuclear Antigen/metabolism , Rhizome/chemistry , Tumor Cells, CulturedABSTRACT
Gastrointestinal leiomyomas are commonly found in the stomach, and but 3% of them arises from the colon. Colonic leiomyomas are often found incidentally during colonoscopic examination. Most of patients with colonic leiomyoma are asymptomatic, but patients with large size leiomyoma occasionally have abdominal mass, hemorrhage, intestinal obstruction or perforation. Pedunculated leiomyomatous polyp is rare. Leiomyomatous polyps are occasionally misjudged as adenomatous polyps by endoscopist. We report a case of pedunculated leiomyomatous colonic polyp in 65 year-old female patient. She has complained of intermittent hematochezia and lower abdominal discomfort for 5 months. During colonoscopy, an 1 cm sized long pedunculated polyp at the splenic flexure was detected. It was removed by colonoscopic snare polypectomy. Leiomyomatous polyp was confirmed by microscopic and immunohistochemical findings.
Subject(s)
Colonic Neoplasms/diagnosis , Colonoscopy , Gastrointestinal Hemorrhage/etiology , Leiomyoma , Leiomyoma/diagnosis , Aged , Anal Canal , Colonic Neoplasms/complications , Female , Humans , Leiomyoma/complicationsABSTRACT
Gastritis cystica profunda is usually characterized by hyperplastic and cystic dilatation of pseudopyloric gland with submucosal invasion. These lesions possess malignant histologic features as metaplastic and even dysplastic alteration can be observed, however they usually exhibit benign behavior. Macroscopically, gastritis cystica profunda may present as a submucosal tumor or as a polyp. In rare case, however, it can be found as a giant mucosal fold. The proposed pathogenesis of lesion relates to ischemia, chronic inflammation, and the presence of foreign body. Herein, we report a case of gastritis cystica profunda associated with high grade tubular adenoma in a patient without antecedent surgery.
