[Effect of Epstein-Barr virus-encoded latent membrane protein 1 on ß-catenin transcriptional activity and expression in nasopharyngeal carcinoma].

OBJECTIVE: To investigate the contribution of latent membrane protein (LMP)1 to nasopharyngeal carcinogenesis via Wnt/ß-catenin signal pathway. METHODS: The recombinant plasmid pHA2-LMP1 was constructed; immunofluorescence staining, Dual-Luciferase Reporter Assay, Western blot and immunohistochemistry staining were used to study the effect of LMP1 on the transcriptional activity and expression of ß-catenin. RESULTS: (1) Abnormal expression of ß-catenin was obtained in 38 cases (50.7%, 38/75), LMP1 expression was obtained in 38 cases (50.7%, 38/75). There was significantly positive correlation between LMP1 expression and abnormal expression of ß-catenin in nasopharyngeal carcinoma tissue (P = 0.008). (2) The expression of ß-catenin in nuclei of NPC cell line CNE1 and CNE2 transfected with pHA2-LMP1 plasmid dramatically increased, and the expression was remarkable in poorly-differentiated NPC cell line CNE2 than that of well-differentiated CNE1 cells. (3) LMP1 expression dramatically increased the transcriptional activity of ß-catenin in CNE1 and CNE2 cells transfected with pHA2-LMP1 and was in a time-dependent. The transcriptional activity of ß-catenin was higher in poorly-defferentiated cell line CNE2 than that of well-differentiated NPC cell line CNE1. (4) LMP1 expression did not affect the total protein expression level of ß-catenin in both CNE1 and CNE2 cell lines. CONCLUSION: EB virus-encoded LMP1 may be involved in the pathogenesis of NPC via ß-catenin signal pathway.

Synthesis, crystal structure, antibacterial assay and DNA binding activity of new binuclear Cu(II) complexes with bridging oxamidate.

Two new binuclear copper complexes, [Cu(2)(oxpn)(bpy)(pic)(H(2)O)](pic) (1) and [Cu(2)(oxpn)(Me(2)bpy)(pic)](pic) (2) [H(2)oxpn=N,N'-bis(3-aminopropyl)oxamide; Hpic=2,4,6-trinitrophenol; bpy=2,2'-bipyridine; Me(2)bpy=4,4'-dimethyl-2,2'-bipyridine], have been synthesized and characterized by elemental analyses, conductivity measurements, IR, UV-visible spectroscopy and single crystal X-ray analyses. Both complexes have similar molecular structures. In complex 1, the central two Cu(II) atoms are bridged by cis-oxpn(2-) with the Cu1-Cu2 separation of 5.221A and the polyhedron of each copper atom is a square-pyramid. Similarly, complex 2 is a cis-oxpn(2-)-bridged binuclear complex with the Cu1-Cu2 separation of 5.196A. Cu1(II) central atom situated in a tetrahedral geometry is four-coordinated and Cu(II) atom situated in a square-pyramidal geometry is five-coordinated. Hydrogen bonding interactions and pi-pi stacking interactions link the binuclear copper complex 1 or 2 into a 2D infinite network. The antibacterial assays indicate that the two complexes showed better activities than their ligands. The interactions of the two binuclear complexes with herring sperm DNA (HS-DNA) have been studied by UV absorption titration, fluorescence titration and viscosity measurements. The results suggest that the two binuclear complexes bind to HS-DNA via an intercalative mode.

{mu-N,N'-Bis[3-(dimethylamino)propyl]oxamidato(2-)-kappa(6)N,N',O':N'',N''',O}bis[(1H-imidazole-kappaN3)(methanol-kappaO)copper(II)] bis(perchlorate).

The structure of the title compound, [Cu2(C12H24N4O2)(C3H4N2)2(CH4O)2](ClO4)2 or [Cu2(dmoxpn)(HIm)2(CH3OH)2](ClO4)2, where dmoxpn is the dianion of N,N'-bis[3-(dimethylamino)propyl]oxamide and HIm is imidazole, consists of a centrosymmetric trans-oxamidate-bridged copper(II) binuclear cation, having an inversion centre at the mid-point of the central C-C bond, and two perchlorate anions. The Cu(II) atom has square-pyramidal coordination geometry involving two N atoms and an O atom from the dmoxpn ligand, an N atom from an imidazole ring, and an O atom from a methanol molecule. The crystal structure is stabilized by O-H...O, N-H...O and C-H...O hydrogen bonds and imidazole pi-pi stacking interactions to form a three-dimensional supramolecular array.

Synthesis and luminescence properties of lanthanide complexes with a new aryl amide bifunctional bridging ligand.

A new aryl amide type bifunctional bridging ligand 4,4'-bis{[(2''-benzylaminoformyl)phenoxyl]methyl}-1,1'-biphenyl (L) and its complexes with lanthanide ions (Ln=Pr, Eu, Gd, Tb, Ho, Er) were synthesized and characterized by elemental analysis, infrared spectra, conductivity measurements and thermal analysis. At the same time, the luminescence properties of the Eu and Tb complexes in acetone solutions were investigated. Under the excitation of UV light, these two complexes exhibited characteristic emission of europium and terbium ions. And the lowest triplet state energy level T1 of this ligand matches better to the lowest resonance energy level of Tb(III) than to Eu(III) ion.