ABSTRACT
By Silica gel, Sephadex LH-20 and other materials for isolation and purification and by physicochemical methods and spectral analysis for structural identification, 32 compounds were isolated and identified from ethyl acetate portion of alcohol extract of the Osmanthus fragrans. Their structures were identified as boschniakinic acid (1), ursolaldehyde (2), augustic acid (3), arjunolic acid (4), 5-hydroxymethyl-2-furancarboxaldehyde (5), isoscutellarein (6), 6, 7-dihydroxycoumarin (7), 2α-hydroxy-oleanolic acid (8), quercetin-3-0-ß-D-glu-copyranoside (9), D-allito (10), 5, 4'-dihydroxy-7- methoxyflavone-3-0-ß-D-glucopyranoside (11), 5,7-dihydroxychromone (12), lupeol (13), naringenin (14), acetyloleanolic acid (15), chlorogenic acid (16), kaempferol-3-0-ß- D-glucopyranoside (17), oleanolic acid (18), kaempferol-3-0-ß-D-galactopyanoside (19), 3', 7-dihydroxy-4'-methoxyisoflavon (20), ergosta-4,6,8 (14), 22-tetraen-3-one (21), p-hydroxycinnamic acid (22), syringaresinol (23), 3,4-dihydroxyacetophenonel (24), ß-sitosterol (25), ethyl p-hydroxyphenylacetate (26), benzoic acid (27), caffeic acid (28), coelonin (29), p-hydorxy-phenylacetic acid (30), p-hydroxyacetophenone (31), and methyl-p-hydroxphenylacetate (32). Except for compounds 2, 4, 5, 8-11, 13, 15, 18, 20, 25, and 27, the rest were isolated from the Osmanthus fragrans for the first time.
Subject(s)
Drugs, Chinese Herbal/chemistry , Oleaceae/chemistry , Drugs, Chinese Herbal/isolation & purification , Molecular Structure , Plants, Medicinal/chemistry , Spectrometry, Mass, Electrospray IonizationABSTRACT
OBJECTIVE: To study the chemical constituents of Citrus medica fruit. METHODS: The fruit of Citrus medica was extracted with 95% EtOH, and the compounds were separated and purified by silica gel, RP-18 and Sephadex LH-20 column chromatography. The structures were identified by spectroscopic analysis. RESULTS: A total of 16 compounds were isolated and identified, including methyl ferulic acid (1), dihydro-N-caffeoyltyramine (2) acacetin (3), ß-ecdysterone (4), (-)-balanophonin (5), p-methoxy cinnamic acid (6), umbelliferone (7), ferulic acid (8), pyrocatechualdehyde (9), diosmetin (10), 4-methoxy salicylic acid (1), ß-amyrin acetate (12), epigallocatechin (13), betulinic acid (14), lupeol (15) and nicotinamide (16). CONCLUSION: All the compounds are isolated from the fruit of Citrus medica for the first time.
Subject(s)
Citrus/chemistry , Fruit/chemistry , Phytochemicals/analysis , Caffeic Acids/isolation & purification , Cinnamates/isolation & purification , Coumaric Acids/isolation & purification , Flavones/isolation & purification , Flavonoids/isolation & purification , Oleanolic Acid/analogs & derivatives , Oleanolic Acid/isolation & purification , Pentacyclic Triterpenes/isolation & purification , Phytochemicals/isolation & purification , Plants, Medicinal/chemistry , Tyramine/analogs & derivatives , Tyramine/isolation & purificationABSTRACT
OBJECTIVE: To study the chemical constituents of aerial parts of Paris polyphylla var. chinensis . METHODS: Aerial parts of Paris polyphylla var. chinensis was extracted with 95% EtOH, and separated and purified by silica gel, RP 18 and Sephadex LH-20 col- umn chromatography. The structures were identified by spectroscopic analysis. RESULTS: A total of ten compounds were isolated and iden- tified as ß-sitosterol (1) ergosta-7, 22-dien-3-one (2), ß-ecdysone (3), kaempferol (4), daucosterol (5) luteolin (6) calonysterone (7), luteolin-7-O-glucoside (8), quercetin (9), and 3ß, 5α, 9α-trihydroxyergosta-7, 22-dien-6-one (10). CONCLUSION: Compounds 2,6 and 10 are isolated from Paris polyphylla var. chinensis for the first time.
