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1.
Mol Biol Rep ; 40(1): 377-82, 2013 Jan.
Article in English | MEDLINE | ID: mdl-23054010

ABSTRACT

We tried to study the possible effects of lipoic acid (LA) on adhesion molecule expression and its underlying mechanism in the prevention and treatment of cardiovascular disorders. Intercellular adhesion molecule-1 (ICAM-1) expression and endothelial nitric oxide synthase (eNOS) activity were determined after endothelial cells were exposed to high glucose in the absence and presence of LA. Coincubation of endothelial cells with high glucose for 24 h resulted in a significant increase of monocyte-endothelial cell adhesion and the expression of ICAM-1 (P < 0.01). These effects were abolished by LA and LA significantly increased eNOS activities (P < 0.01). These findings suggested that LA may play a role in inhibiting expression of adhesion molecules by increasing eNOS activities.


Subject(s)
Antioxidants/pharmacology , Cell Adhesion Molecules/metabolism , Endothelial Cells/drug effects , Endothelial Cells/metabolism , Thioctic Acid/pharmacology , Cell Adhesion/drug effects , Cells, Cultured , Enzyme Activation , Glucose/metabolism , Humans , Intercellular Adhesion Molecule-1/metabolism , Monocytes/drug effects , Monocytes/metabolism , Nitric Oxide Synthase Type III/metabolism
2.
Chinese Medical Journal ; (24): 655-658, 2013.
Article in English | WPRIM (Western Pacific) | ID: wpr-342522

ABSTRACT

<p><b>BACKGROUND</b>Oral squamous cell carcinoma (OSCC) is one of the most common malignancies in the oral and maxillofacial region. Yes-associated protein 1 (YAP1) has been implicated as a bona fide oncogene in solid tumors. We seek to elucidate the role of YAP1 in OSCC tissue.</p><p><b>METHODS</b>We identified YAP1 gene and protein overexpression in 30 OSCC patients and 10 normal oral mucosa tissues by immunohistochemistry, Western blotting and reverse transcription polymerase chain reaction (RT-PCR).</p><p><b>RESULTS</b>In the normal oral mucosa by immunohistochemical staining, YAP1 mainly located in both the cytoplasm and nucleus mainly the nuclei of the basal cells. In OSCC, the expression of YAP1 translocated from the nucleus to cytoplasm; YAP1 being mainly located in both the cytoplasm and nucleus of the adjacent mucosa. The expression of YAP1 gradual increased in normal oral mucosa, tumor adjacent mucosa and low grade, middle grade, high grade OSCC tissue by Western blotting. Significant difference was found between the expressions of the normal oral mucosa and OSCC tissue (P < 0.05). The coincidence was detected between the normal oral mucosa and OSCC tissue by RT-PCR (P < 0.05).</p><p><b>CONCLUSIONS</b>YAP1 is involved in the carcinogenesis and development of OSCC. There is a transformation between nucleus and cytoplasm.</p>


Subject(s)
Humans , Adaptor Proteins, Signal Transducing , Genetics , Metabolism , Blotting, Western , Carcinoma, Squamous Cell , Genetics , Metabolism , In Vitro Techniques , Mouth Neoplasms , Genetics , Metabolism , Phosphoproteins , Genetics , Metabolism , Reverse Transcriptase Polymerase Chain Reaction
3.
Mol Biol Rep ; 39(12): 11005-9, 2012 Dec.
Article in English | MEDLINE | ID: mdl-23053990

ABSTRACT

Myocardial ischemia and reperfusion (MI/R) is associated with an intense inflammatory reaction, which may lead to myocyte injury. In this study, we investigated the effect of quercetin, an inhibitor of c-Jun N-terminal kinase on ischemia/reperfusion injury in isolated rat hearts. Rat models of MI/R were induced by coronary occlusion followed by reperfusion, treatment of rats with quercetin (1.0 mg/kg, i.v.) induced a significant reduction of infarct volume and improvements in baseline hemodynamic abnormalities (P < 0.05). Quercetin treatment also attenuated the expression of both TNF-alpha (TNF-α) and interleukin-10 (IL-10) and lowered the serum levels of inflammatory cytokine (P < 0.05). These findings suggested that quercetin treatment significantly attenuated MI/R injury primarily through anti-inflammatory effects.


Subject(s)
Myocardial Reperfusion Injury/drug therapy , Protective Agents/therapeutic use , Quercetin/therapeutic use , Animals , Gene Expression Regulation/drug effects , Hemodynamics/drug effects , Interleukin-10/metabolism , Male , Myocardial Infarction/drug therapy , Myocardial Infarction/genetics , Myocardial Infarction/pathology , Myocardial Infarction/physiopathology , Myocardial Reperfusion Injury/genetics , Myocardial Reperfusion Injury/physiopathology , Myocardium/metabolism , Myocardium/pathology , Protective Agents/pharmacology , Quercetin/pharmacology , RNA, Messenger/genetics , RNA, Messenger/metabolism , Rats , Rats, Sprague-Dawley , Tumor Necrosis Factor-alpha/genetics , Tumor Necrosis Factor-alpha/metabolism
4.
Inflammation ; 35(6): 1867-71, 2012 Dec.
Article in English | MEDLINE | ID: mdl-22814938

ABSTRACT

Downstream regulatory element antagonistic modulator (DREAM) is a critical transcriptional repressor for pain modulation. The role of nitric oxide (NO) plays in modulating DREAM pain pathway in the periphery is unclear. Therefore, we investigated the role of the NO in modulation of the expression of DREAM in formalin-induced rat inflammatory pain models. Male Sprague-Dawley rats were randomly distributed into four groups: the normal group, formalin test group, Nω-nitro-L-arginine (l-NNA) group, and morphine group. One hundred microliters of 2.5 % formalin was injected into the plantar surface of the right hindpaw of rats. l-NNA (40 nmol/L) and morphine (40 nmol/L) were injected intrathecally in the hindpaw before formalin injection. The nociceptive behavioral reaction was recorded. After the formalin test, the expression of DREAM mRNA and protein in the spinal cord of the four groups were measured. The nociceptive reaction induced by injection of formalin exhibited two phases. Morphine and l-NNA significantly decreased pain scores of the second phase. The expression of DREAM was significantly increased in the rat spinal cord after formalin-induced pain. Morphine significantly upregulated the expression of DREAM, and the formalin-induced upregulation was significantly attenuated by l-NNA. NO may play an important role in the DREAM pathway modulation of inflammatory pain.


Subject(s)
Inflammation/physiopathology , Kv Channel-Interacting Proteins/metabolism , Nitric Oxide/metabolism , Pain/physiopathology , Repressor Proteins/metabolism , Animals , Formaldehyde , Inflammation/chemically induced , Injections, Spinal , Kv Channel-Interacting Proteins/genetics , Male , Morphine/pharmacology , Morphine/therapeutic use , Nitroarginine/pharmacology , Nitroarginine/therapeutic use , Pain/chemically induced , Pain/drug therapy , Pain/metabolism , Pain Measurement , RNA, Messenger/genetics , RNA, Messenger/metabolism , Random Allocation , Rats , Rats, Sprague-Dawley , Repressor Proteins/genetics , Spinal Cord/metabolism
5.
Article in Chinese | WPRIM (Western Pacific) | ID: wpr-271514

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the expression of JAK, ERK and Cyclin D proteins in squamous-cell carcinoma of tongue.</p><p><b>METHODS</b>The expression of JAK, ERK and Cyclin D1 proteins was determined with SP immunohistochemical method in 30 cases of lingual Squamous cell carcinoma, 20 of normal lingual mucosa, 10 of mild epithelial dysplasia and 20 of severe epithelial dysplasia.</p><p><b>RESULTS</b>The expression of pJAK in lingual squamous-cell carcinoma and epithelial dysplasia was stronger than that of normal lingual mucosa (chi2=37.54, P<0.01), and the expression of pJAK in lingual squamous-cell carcinoma was significantly higher than that of the epithelial dysplasia (chi2=6.83, P<0.05). pJAK expression in squamous-cell carcinoma of low-middle differentiation was stronger than that of high differentiation. There was no significant difference in pERK expression among lingual squamous-cell carcinoma, normal lingual mucosa and epithelial dysplasia. There was a significantly positive correlation between pJAK and Cyclin D1 expression in SCC (r=0.619, P<0.05). There was no significant correlation between the expression of pERK and Cyclin D1 (r=0.231, P>0.05).</p><p><b>CONCLUSION</b>Over-expression of pJAK and Cyclin D1 may be associated with the occurrence and development of squamous-cell carcinoma of the tongue.</p>


Subject(s)
Humans , Carcinoma, Squamous Cell , Metabolism , Pathology , Cyclin D1 , Metabolism , Extracellular Signal-Regulated MAP Kinases , Metabolism , Immunohistochemistry , Janus Kinases , Metabolism , Phosphorylation , Tongue Neoplasms , Metabolism , Pathology
6.
Article in English | WPRIM (Western Pacific) | ID: wpr-316346

ABSTRACT

<p><b>OBJECTIVE</b>To assess the effect of temporary occlusion of hepatic blood inflow on hepatic cancer treated with diode-laser induced thermocogation (LITT).</p><p><b>METHODS</b>The carcinoma Walker-256 was implanted in 40 SD rat livers. Twelve days later, the animals were randomly divided into 4 groups. Group A received LITT alone; group B received hepatic artery temporary occlusion during LITT; group C received portal vein temporary occlusion during LITT; group D received hepatic artery and portal vein temporary occlusion during LITT. Tumors were exposed to 810 nm diode-laser light at 0.95 watts for 10 min from a scanner tip applicator placed in the tumor. At the same time, the intrahepatic temperature distribution in rats with liver tumors was measured per 2 min during thermocoagulation. Tumor control was examined immediately 7 and 14 d after thermocoagulation.</p><p><b>RESULTS</b>There was significant difference of intrahepatic temperature distribution in rats with liver tumors among the 4 groups (P<0.05) except when group C samples were compared with group D samples at each time point, and group B samples were compared with group C samples at 120 s (P>0.05). Light microscopic examination of the histologic section samples revealed three separate zones: regular hyperthermic coagulation necrosis zone, transition zone and reference zone. Compared with the samples in group A and group B, group C and group D samples had more clear margin among the three zones.</p><p><b>CONCLUSION</b>The hepatic blood inflow occlusion, especially portal vein hepatic blood inflow occlusion, or all hepatic blood inflow occlusion considerably increased the efficacy of LITT in the treatment of liver cancer.</p>


Subject(s)
Animals , Rats , Laser Coagulation , Liver Circulation , Physiology , Liver Neoplasms , General Surgery , Temperature , Time Factors
7.
Article in Chinese | WPRIM (Western Pacific) | ID: wpr-355191

ABSTRACT

<p><b>OBJECTIVE</b>To study the expressions of matrix metalloproteinase 2 (MMP2) and E-cadherin (E-CD) in salivary mucoepidermoid carcinoma, and their relationship with clinical stages, pathological grading, lymph node metastasis and prognosis.</p><p><b>METHODS</b>Surgical specimens of salivary mucoepidermiod carcinoma and normal salivary gland tissue were collected. MMP-2 and E-CD were stained immunohistochemically with streptavidin peroxidase method.</p><p><b>RESULTS</b>The expression of MMP-2 was increased and the expression of E-CD was reduced or negative in salivary mucoepidemoid carcinoma compared with those of the normal salivary gland. Expression of MMP-2 and E-CD was closely correlated with lymph node metastasis of the mucoepidermoid carcinoma. MMP-2 was positively correlated with the prognosis of mucoepidermoid carcinoma, and E-CD was negatively correlated to the prognosis of mucoepidermoid carcinoma.</p><p><b>CONCLUSION</b>The expression of MMP-2 and E-CD is closely correlated with the metastasis and prognosis of salivary mucoepidermoid carcinoma.</p>


Subject(s)
Humans , Cadherins , Genetics , Carcinoma, Mucoepidermoid , Metabolism , Pathology , Matrix Metalloproteinase 2 , Genetics , Neoplasm Invasiveness , Neoplasm Metastasis , Prognosis , Salivary Gland Neoplasms , Metabolism , Pathology
8.
Chinese Journal of Surgery ; (12): 849-851, 2003.
Article in Chinese | WPRIM (Western Pacific) | ID: wpr-311192

ABSTRACT

<p><b>OBJECTIVE</b>To seek a safe, efficient, and cost-effective technique for local thermo-ablation of hepatic cancer.</p><p><b>METHODS</b>The livers from 16 healthy rabbits were thermocoagulated by diode-laser with scanner fiber tip, 6 w for 10 mins. At the same time, the temperatures were measured at 0, 5 and 10 mm from laser tip. The pre-thermocoagulative liver function was compared with that of 7 days post-thermocoagulation. The pathologic changes were also observed 1 month after laser thermocoagulation.</p><p><b>RESULTS</b>All the rabbits survived and hepatic tissue temperatures at 0, 5, 10 mm from laser tip reached 96.39 degrees C +/- 3.97 degrees C, 60.79 degrees C +/- 6.21 degrees C, 46.10 degrees C +/- 4.58 degrees C respectively after 10 minutes of thermocoagulation. There were no significant differences in liver function parameters between rabbits of pre-laser thermocoagulation and of post-laser thermocoagulation. Thermocoagulated necrosis of liver tissue with surrounding fibrosis in a diameter of 26.0 mm was formed. Light microscopy revealed coagulative necrosis in the center of the coagulated area without surviving hepatic cells.</p><p><b>CONCLUSION</b>The hepatic tissue can be coagulated safely and effectively by diode-laser with scanner fibertip, and such a technique may provide a new method for the treatment of hepatic carcinoma.</p>


Subject(s)
Animals , Female , Male , Rabbits , Laser Coagulation , Methods , Liver Neoplasms , Pathology , General Surgery
9.
Article in Chinese | WPRIM (Western Pacific) | ID: wpr-289330

ABSTRACT

OBJECTIVE: To study the effect of basic fibroblast growth factor(b-FGF) on revascularization and bone remodeling of allogeneic mandible transplantation in repair of mandible defects in rabbits. METHODS: The mandible defects of 20 adult rabbits were created in both sides. The defects on the left side were implanted with allogeneic bone and local administration of b-FGF; the defects on the right side were only repaired with allogeneic bone as control group. At 1, 3 months after operation, the revascularization and bone remodeling were observed by ink-gelation vascular perfusion-transparency and histological examination. RESULTS: The allogeneic bone and b-FGF group had more marked vascularization and more quick and complete bone formation than control group. CONCLUSION: b-FGF can improve revascularization and bone formation after allogeneic mandible transplantation; allogeneic bone combined with b-FGF is a promising bone substitute in clinical uses.

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