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1.
J Biol Inorg Chem ; 23(7): 1085-1092, 2018 10.
Article in English | MEDLINE | ID: mdl-30251130

ABSTRACT

The binding of neutral thiol (ethanethiol, EtSH) or thioether (tetrahydrothiophene, THT) to two types of heme proteins in their ferrous state has been investigated with UV-visible (UV-Vis) absorption and magnetic circular dichroism spectroscopy. For the second GAF (cGMP-specific phosphodiesterases, adenylyl cyclases, and FhlA) domain from the sensory kinase MsmS (sGAF2), stepwise additions of these respective two sulfur-donor ligands to its dithionite-reduced ferrous form generate homogeneous six-coordinate low-spin ferrous complexes at both pHs 7.0 and 5.4. Similar complexes were partially formed for deoxyferrous soybean leghemoglobin with EtSH or THT within their solubility limits in water. The titrations cause significant UV-Vis spectra changes attributable to a five-coordinate to six-coordinate heme iron coordination change. For sGAF2, the resulting spectra are essentially identical for the both ligands, clearly indicating the direct binding of neutral thiol/thioether to ferrous heme iron as the distal ligand. On the other hand, the thiol EtSH binds to ferric sGAF2 in the anionic thiolate form, while thioether THT forms its ferric sGAF2 complex as a neutral ligand. These observations provide compelling evidence that neutral cysteine is a plausible ligand for ferrous heme proteins.


Subject(s)
Coordination Complexes/chemistry , Ferrous Compounds/chemistry , Heme/chemistry , Sulfhydryl Compounds/chemistry , Coordination Complexes/chemical synthesis , Ligands , Molecular Structure , Spectrophotometry, Ultraviolet
2.
Nat Chem Biol ; 11(8): 598-605, 2015 Aug.
Article in English | MEDLINE | ID: mdl-26075523

ABSTRACT

Plants synthesize carotenoids, which are essential for plant development and survival. These metabolites also serve as essential nutrients for human health. The biosynthetic pathway for all plant carotenoids occurs in chloroplasts and other plastids and requires 15-cis-ζ-carotene isomerase (Z-ISO). It was not known whether Z-ISO catalyzes isomerization alone or in combination with other enzymes. Here we show that Z-ISO is a bona fide enzyme and integral membrane protein. Z-ISO independently catalyzes the cis-trans isomerization of the 15-15' carbon-carbon double bond in 9,15,9'-cis-ζ-carotene to produce the substrate required by the subsequent biosynthetic-pathway enzyme. We discovered that isomerization depends upon a ferrous heme b cofactor that undergoes redox-regulated ligand switching between the heme iron and alternate Z-ISO amino acid residues. Heme b-dependent isomerization of a large hydrophobic compound in a membrane was previously undescribed. As an isomerase, Z-ISO represents a new prototype for heme b proteins and potentially uses a new chemical mechanism.


Subject(s)
Arabidopsis Proteins/metabolism , Heme/metabolism , Iron/metabolism , Membrane Proteins/metabolism , Plant Proteins/metabolism , Zea mays/chemistry , cis-trans-Isomerases/metabolism , zeta Carotene/biosynthesis , Arabidopsis/chemistry , Arabidopsis/enzymology , Arabidopsis Proteins/chemistry , Arabidopsis Proteins/genetics , Chloroplasts/genetics , Chloroplasts/metabolism , Escherichia coli/genetics , Escherichia coli/metabolism , Gene Expression , Heme/chemistry , Hydrophobic and Hydrophilic Interactions , Iron/chemistry , Isoenzymes/chemistry , Isoenzymes/genetics , Isoenzymes/metabolism , Isomerism , Membrane Proteins/chemistry , Membrane Proteins/genetics , Models, Molecular , Oxidation-Reduction , Plant Proteins/chemistry , Plant Proteins/genetics , Recombinant Proteins/chemistry , Recombinant Proteins/genetics , Recombinant Proteins/metabolism , Zea mays/enzymology , Zea mays/genetics , cis-trans-Isomerases/chemistry , cis-trans-Isomerases/genetics
3.
Biochemistry ; 53(30): 4894-903, 2014 Aug 05.
Article in English | MEDLINE | ID: mdl-25046203

ABSTRACT

The fleeting ferric peroxo and hydroperoxo intermediates of dioxygen activation by hemoproteins can be readily trapped and characterized during cryoradiolytic reduction of ferrous hemoprotein-O2 complexes at 77 K. Previous cryoannealing studies suggested that the relaxation of cryogenerated hydroperoxoferric intermediates of myoglobin (Mb), hemoglobin, and horseradish peroxidase (HRP), either trapped directly at 77 K or generated by cryoannealing of a trapped peroxo-ferric state, proceeds through dissociation of bound H2O2 and formation of the ferric heme without formation of the ferryl porphyrin π-cation radical intermediate, compound I (Cpd I). Herein we have reinvestigated the mechanism of decays of the cryogenerated hydroperoxyferric intermediates of α- and ß-chains of human hemoglobin, HRP, and chloroperoxidase (CPO). The latter two proteins are well-known to form spectroscopically detectable quasistable Cpds I. Peroxoferric intermediates are trapped during 77 K cryoreduction of oxy Mb, α-chains, and ß-chains of human hemoglobin and CPO. They convert into hydroperoxoferric intermediates during annealing at temperatures above 160 K. The hydroperoxoferric intermediate of HRP is trapped directly at 77 K. All studied hydroperoxoferric intermediates decay with measurable rates at temperatures above 170 K with appreciable solvent kinetic isotope effects. The hydroperoxoferric intermediate of ß-chains converts to the S = 3/2 Cpd I, which in turn decays to an electron paramagnetic resonance (EPR)-silent product at temperature above 220 K. For all the other hemoproteins studied, cryoannealing of the hydroperoxo intermediate directly yields an EPR-silent majority product. In each case, a second follow-up 77 K γ-irradiation of the annealed samples yields low-spin EPR signals characteristic of cryoreduced ferrylheme (compound II, Cpd II). This indicates that in general the hydroperoxoferric intermediates relax to Cpd I during cryoanealing at low temperatures, but when this state is not captured by reaction with a bound substrate, it is reduced to Cpd II by redox-active products of radiolysis.


Subject(s)
Cryopreservation/methods , Hemeproteins/chemistry , Hemeproteins/metabolism , Electron Spin Resonance Spectroscopy/methods , Ferric Compounds/analysis , Ferric Compounds/chemistry , Ferric Compounds/metabolism , Hemoglobins/chemistry , Hemoglobins/metabolism , Humans , Hydrogen Peroxide/analysis , Hydrogen Peroxide/chemistry , Hydrogen Peroxide/metabolism
4.
Biochemistry ; 53(30): 4956-69, 2014 Aug 05.
Article in English | MEDLINE | ID: mdl-24972312

ABSTRACT

The coelomic O2-binding hemoglobin dehaloperoxidase (DHP) from the sea worm Amphitrite ornata is a dual-function heme protein that also possesses a peroxidase activity. Two different starting oxidation states are required for reversible O2 binding (ferrous) and peroxidase (ferric) activity, bringing into question how DHP manages the two functions. In our previous study, the copresence of substrate 2,4,6-trichlorophenol (TCP) and H2O2 was found to be essential for the conversion of oxy-DHP to enzymatically active ferric DHP. On the basis of that study, a functional switching mechanism involving substrate radicals (TCP(•)) was proposed. To further support this mechanism, herein we report details of our investigations into the H2O2-mediated conversion of oxy-DHP to the ferric or ferryl ([TCP] < [H2O2]) state triggered by both biologically relevant [TCP and 4-bromophenol (4-BP)] and nonrelevant (ferrocyanide) compounds. At <50 µM H2O2, all of these conversion reactions are completely inhibited by ferric heme ligands (KCN and imidazole), indicating the involvement of ferric DHP. Furthermore, the spin-trapping reagent 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) effectively inhibits the TCP/4-BP (but not ferrocyanide)-triggered conversion of oxy-DHP to ferric DHP. These results and O2 concentration-dependent conversion rates observed in this study demonstrate that substrate TCP triggers the conversion of oxy-DHP to a peroxidase by TCP(•) oxidation of the deoxyferrous state. TCP(•) is progressively generated, by increasingly produced amounts of ferric DHP, upon H2O2 oxidation of TCP catalyzed initially by trace amounts of ferric enzyme present in the oxy-DHP sample. The data presented herein further address the mechanism of how the halophenolic substrate triggers the conversion of hemoglobin DHP into a peroxidase.


Subject(s)
Chlorophenols/chemistry , Ferric Compounds/chemistry , Hemoglobins/chemistry , Oxygen/chemistry , Peroxidase/chemistry , Animals , Hemoglobins/physiology , Oxygen/physiology , Peroxidase/physiology , Polychaeta , Substrate Specificity
5.
Arch Biochem Biophys ; 545: 108-15, 2014 Mar 01.
Article in English | MEDLINE | ID: mdl-24440609

ABSTRACT

Sea worm, Amphitrite ornata, has evolved its globin (an O(2) carrier) also to serves as a dehaloperoxidase (DHP) to detoxify haloaromatic pollutants generated by competing species. A previous mutagenesis study by our groups on both DHP and sperm whale myoglobin (SW Mb) revealed some structural factors that influence the dehaloperoxidase activities (significantly lower for Mb) of both proteins. Using an isocyanide/O(2) partition constant measurement method in this study, we have examined the effects of these structural factors on the O(2) equilibrium constants (KO2) of DHP, SW Mb, and their mutants. A clear trend of decreasing O(2) affinity and increasing catalytic activity along with the increase in the distal His N(ε)-heme iron distance is observed. An H93K/T95H Mb double mutant mimicking the DHP proximal His positioning exhibited markedly enhanced O(2) affinity, confirming the essential effect of proximal His rotation on the globin function of DHP. For DHP, the L100F, T56G and M86E variants showed the effects of distal volume, distal His flexibility and proximal electronic push, respectively, on the O(2) affinity. This study provides insights into how DHP has evolved its heme environment to gain significantly enhanced peroxidase capability without compromising its primary function as an O(2) carrier.


Subject(s)
Heme/chemistry , Myoglobin/metabolism , Oxygen/metabolism , Peroxidases/metabolism , Polychaeta/enzymology , Animals , Crystallography, X-Ray , Heme/metabolism , Models, Molecular , Myoglobin/chemistry , Peroxidases/chemistry , Polychaeta/chemistry , Polychaeta/metabolism , Protein Conformation , Sperm Whale/metabolism
6.
J Inorg Biochem ; 127: 238-45, 2013 Oct.
Article in English | MEDLINE | ID: mdl-23639797

ABSTRACT

To further investigate the properties of phosphines as structural and functional probes of heme proteins, mono- and bis-phosphine [tris(hydroxymethyl)phosphine, THMP] adducts of H93G myoglobin (Mb) have been prepared by stepwise THMP titrations of exogenous ligand-free ferric and ferrous H93G Mb, respectively. Bubbling with CO or stepwise titration with imidazole (Im) of the bis-THMP-ligated ferrous protein generated a mixed ligand (THMP/CO or THMP/Im, respectively) ferrous complexes. Stable oxyferrous H93G(THMP) Mb was formed at -40°C by bubbling the mono-THMP-Fe(II) protein with O2. A THMP-ligated ferryl H93G Mb moiety has been partially formed upon addition of H2O2 to the ferric mono-THMP adduct. All the species prepared above have been characterized with UV-visible (UV-vis) absorption and magnetic circular dichroism (MCD) spectroscopy in this study. The six-coordinate ferrous bis-phosphine and mono-phosphine/CO complexes of H93G Mb exhibit characteristic spectral features (red-shifted Soret/unique-shaped MCD visible bands and hyperporphyrin spectra, respectively) that only have been seen for the analogous phosphine or CO-complexes of thiolate-ligated heme proteins such as cytochrome P450 (P450) and Caldariomyces fumago chloroperoxidase (CPO). However, such resemblance is not seen in phosphine-ligated ferric H93G Mb even though phosphine-bound ferric P450 and CPO display hyperporphyrin spectra. In fact, bis-THMP-bound ferric H93G Mb exhibits MCD and UV-vis absorption spectra that are similar to those of bis-amine- and bis-thioether-ligated H93G Mb complexes. This study also further demonstrates the utility of the H93G cavity mutant for preparing novel heme iron coordination structures.


Subject(s)
Carbon Monoxide/chemistry , Coordination Complexes/chemistry , Cytochrome P-450 Enzyme System/chemistry , Ferrous Compounds/chemistry , Models, Biological , Phosphines/chemistry , Molecular Structure
7.
J Inorg Biochem ; 117: 316-21, 2012 Dec.
Article in English | MEDLINE | ID: mdl-23102773

ABSTRACT

Horseradish peroxidase (HRP) catalyzes the oxidative para-dechlorination of the environmental pollutant/carcinogen 2,4,6-trichlorophenol (2,4,6-TCP). A possible mechanism for this reaction is a direct oxygen atom transfer from HRP compound I (HRP I) to trichlorophenol to generate 2,6-dichloro 1,4-benzoquinone, a two-electron transfer process. An alternative mechanism involves two consecutive one-electron transfer steps in which HRP I is reduced to compound II (HRP II) and then to the ferric enzyme as first proposed by Wiese et al. [F.W. Wiese, H.C. Chang, R.V. Lloyd, J.P. Freeman, V.M. Samokyszyn, Arch. Environ. Contam. Toxicol. 34 (1998) 217-222]. To probe the mechanism of oxidative halophenol dehalogenation, the reactions between 2,4,6-TCP and HRP compounds I or II have been investigated under single turnover conditions (i.e., without excess H(2)O(2)) using rapid scan stopped-flow spectroscopy. Addition of 2,4,6-TCP to HRP I leads rapidly to HRP II and then more slowly to the ferric resting state, consistent with a mechanism involving two consecutive one-electron oxidations of the substrate via a phenoxy radical intermediate. HRP II can also directly dechlorinate 2,4,6-TCP as judged by rapid scan stopped-flow and mass spectrometry. This observation is particularly significant since HRP II can only carry out one-electron oxidations. A more detailed understanding of the mechanism of oxidative halophenol dehalogenation will facilitate the use of HRP as a halophenol bioremediation catalyst.


Subject(s)
Chlorophenols/chemistry , Horseradish Peroxidase/chemistry , Biodegradation, Environmental , Catalysis , Halogenation , Horseradish Peroxidase/metabolism , Oxidation-Reduction
8.
J Inorg Biochem ; 105(12): 1786-94, 2011 Dec.
Article in English | MEDLINE | ID: mdl-22104301

ABSTRACT

Bovine liver catalase (BLC), catalase-related allene oxide synthase (cAOS) from Plexaura homomalla, and a recently isolated protein from the cattle pathogen Mycobacterium avium ssp. paratuberculosis (MAP-2744c (MAP)) are all tyrosinate-ligated heme enzymes whose crystal structures have been reported. cAOS and MAP have low (<20%) sequence similarity to, and significantly different catalytic functions from, BLC. cAOS transforms 8R-hydroperoxy-eicosatetraenoic acid to an allene epoxide, whereas the MAP protein is a putative organic peroxide-dependent peroxidase. To elucidate factors influencing the functions of these and related heme proteins, we have investigated the heme iron coordination properties of these tyrosinate-ligated heme enzymes in their ferric and ferrous states using magnetic circular dichroism and UV-visible absorption spectroscopy. The MAP protein shows remarkable spectral similarities to cAOS and BLC in its native Fe(III) state, but clear differences from ferric proximal heme ligand His93Tyr Mb (myoglobin) mutant, which may be attributed to the presence of an Arg(+)-N(ω)-H···¯O-Tyr (proximal heme axial ligand) hydrogen bond in the first three heme proteins. Furthermore, the spectra of Fe(III)-CN¯, Fe(III)-NO, Fe(II)-NO (except for five-coordinate MAP), Fe(II)-CO, and Fe(II)-O(2) states of cAOS and MAP, but not H93Y Mb, are also similar to the corresponding six-coordinate complexes of BLC, suggesting that a tyrosinate (Tyr-O¯) is the heme axial ligand trans to the bound ligands in these complexes. The Arg(+)-N(ω)-H to ¯O-Tyr hydrogen bond would be expected to modulate the donor properties of the proximal tyrosinate oxyanion and, combined with the subtle differences in the catalytic site structures, affect the activities of cAOS, MAP and BLC.


Subject(s)
Anthozoa/enzymology , Bacterial Proteins/chemistry , Catalase/chemistry , Iron/chemistry , Lipoxygenase/chemistry , Liver/enzymology , Mycobacterium avium subsp. paratuberculosis/enzymology , Peroxidases/chemistry , Amino Acid Substitution , Animals , Carbon Monoxide/chemistry , Catalytic Domain , Cattle , Circular Dichroism , Coordination Complexes/chemistry , Ferric Compounds/chemistry , Ferrous Compounds/chemistry , Humans , Hydrogen Bonding , Myoglobin/chemistry , Myoglobin/genetics , Oxidation-Reduction
9.
Coord Chem Rev ; 255(7-8): 700-716, 2011 Apr 01.
Article in English | MEDLINE | ID: mdl-21423881

ABSTRACT

Preparation of heme model complexes is a challenging subject of long-standing interest for inorganic chemists. His93Gly sperm whale myoglobin (H93G Mb) has the proximal His replaced with the much smaller non-coordinating Gly. This leaves a cavity on the proximal side of the heme into which a wide variety of exogenous ligands can be delivered. The end result is a remarkably versatile scaffold for the preparation of model heme adducts to mimic the heme iron coordination structure of native heme proteins. In this review, we first summarize the quantitative evidence for differential ligand binding affinities of the proximal and distal pockets of the H93G Mb cavity mutant that facilitates the preparation of mixed-ligand derivatives. Then we review our use of magnetic circular dichroism and electronic absorption spectroscopy to characterize nitrogen-, oxygen-, and sulfur-donor-ligated H93G Mb adducts with an emphasis on species not easily prepared by other heme model system approaches and those that serve as spectroscopic models for native heme proteins.

10.
Arch Biochem Biophys ; 507(1): 119-25, 2011 Mar 01.
Article in English | MEDLINE | ID: mdl-21147058

ABSTRACT

All cytochrome P450s (CYPs) contain a cysteinate heme iron proximal ligand that plays a crucial role in their mechanism of action. Conversion of the proximal Cys436 to Ser in NH(2)-truncated microsomal CYP2B4 (ΔCYP2B4) transforms the enzyme into a two-electron NADPH oxidase producing H(2)O(2) without monooxygenase activity [K.P. Vatsis, H.M. Peng, M.J. Coon, J. Inorg. Biochem. 91 (2002) 542-553]. To examine the effects of this ligation change on the heme iron spin-state and coordination structure of ΔC436S CYP2B4, the magnetic circular dichroism and electronic absorption spectra of several oxidation/ligation states of the variant have been measured and compared with those of structurally defined heme complexes. The spectra of the substrate-free ferric mutant are indicative of a high-spin five-coordinate structure ligated by anionic serinate. The spectroscopic properties of the dithionite-reduced (deoxyferrous) protein are those of a five-coordinate (high-spin) state, and it is concluded that the proximal ligand has been protonated to yield neutral serine (ROH-donor). Low-spin six-coordinate ferrous complexes of the mutant with neutral sixth ligands (NO, CO, and O(2)) examined are also likely ligated by neutral serine, as would be expected for ferric complexes with anionic sixth ligands such as the hydroperoxo-ferric catalytic intermediate. Ligation of the heme iron by neutral serine vs. deprotonated cysteine is likely the result of the large difference in their acidity. Thus, without the necessary proximal ligand push of the cysteinate, although the ΔC436S mutant can accept two electrons and two protons, it is unable to heterolytically cleave the O-O bond of the hydroperoxo-ferric species to generate Compound I and hydroxylate the substrate.


Subject(s)
Aryl Hydrocarbon Hydroxylases/genetics , Aryl Hydrocarbon Hydroxylases/metabolism , Cysteine/metabolism , Heme/metabolism , Oxygen/metabolism , Point Mutation , Animals , Aryl Hydrocarbon Hydroxylases/chemistry , Carbon Monoxide/metabolism , Circular Dichroism , Cysteine/genetics , Cytochrome P450 Family 2 , Heme/chemistry , Iron/metabolism , Ligands , Models, Molecular , Nitrogen Oxides/metabolism , Spectrophotometry , Sulfur/metabolism
11.
Biochim Biophys Acta ; 1814(1): 69-75, 2011 Jan.
Article in English | MEDLINE | ID: mdl-20637316

ABSTRACT

Mammalian cytochrome P450 2B4 (CYP2B4) is a phenobarbital-inducible rabbit hepatic monooxygenase that catalyzes the N-demethylation of benzphetamine and metabolism of numerous other compounds. To probe the interactions of the heme environment and bound benzphetamine with the dioxygen (O2) complex of CYP2B4, homogeneous O2 complexes of the wild-type enzyme and three mutants at sites of conserved amino acids, two on the heme distal side (T302A and E301Q) and one on the proximal side (F429H), have been prepared and stabilized at ~-50°C in mixed solvents (60-70% v/v glycerol). We report that the magnetic circular dichroism and electronic absorption spectra of wild-type oxyferrous CYP2B4, in the presence and absence of substrate, are quite similar to those of the dioxygen complex of bacterial cytochrome P450-CAM (CYP101). However, the oxyferrous complexes of the T302A and E301Q CYP2B4 mutants have significantly perturbed electronic structure (~4 nm and ~3 nm red-shifted Soret features, respectively) compared to that of the wild-type oxyferrous complex. On the other hand, the heme proximal side mutant, CYP2B4 F429H, undergoes relatively facile conversion to a partially (~50%) denatured (P420) form upon reduction. The structural changes in the heme pocket environments of the CYP2B4 mutants that lead to the spectroscopic distinctions reported herein can be related to the differences in oxidation activities of wild-type CYP2B4 and its E301Q, T302A and F429H mutants.


Subject(s)
Aryl Hydrocarbon Hydroxylases/genetics , Aryl Hydrocarbon Hydroxylases/metabolism , Mutation , Oxygen/metabolism , Amino Acid Substitution , Animals , Aryl Hydrocarbon Hydroxylases/chemistry , Benzphetamine/chemistry , Benzphetamine/metabolism , Catalytic Domain , Circular Dichroism , Cold Temperature , Cytochrome P450 Family 2 , Heme/chemistry , Heme/metabolism , Iron/chemistry , Iron/metabolism , Models, Molecular , Organometallic Compounds/chemistry , Organometallic Compounds/metabolism , Oxygen/chemistry , Protein Binding , Protein Structure, Tertiary , Rabbits , Spectrophotometry , Substrate Specificity
12.
Biochemistry ; 49(29): 6064-9, 2010 Jul 27.
Article in English | MEDLINE | ID: mdl-20565134

ABSTRACT

Amphitrite ornata dehaloperoxidase (DHP) is the first heme-containing globin possessing a native peroxidase enzymatic activity. DHP catalyzes the H(2)O(2)-dependent dehalogenation of halophenols. By possessing this detoxifying enzymatic activity, these organisms are able to thrive in an environment contaminated with toxic haloaromatics. It has been proposed that DHP evolved from a dioxygen carrier globin protein and therefore possesses dual physiological roles of O(2) carrier and dehaloperoxidase. Although DHP is isolated in the catalytically inactive oxyferrous state (oxy-DHP), we find that the combination of H(2)O(2) and the substrate 2,4,6-trichlorophenol (TCP) brings about facile switching of oxy-DHP to the enzymatically active ferric state via a process likely involving substrate radicals (TCP*). In contrast, in the absence of TCP, H(2)O(2) alone converts oxy-DHP to an inactive state (compound RH) instead of oxidizing the enzyme to the ferric state. Further, although the rate of autoxidation of oxy-DHP is somewhat enhanced by the presence of TCP, the effect is too small to be the functional switch. Instead, both substrate and H(2)O(2) are needed to convert oxy-DHP to the catalytically active ferric state. These observations provide a physiological link between the O(2) carrier role of the ferrous protein and the peroxidase activity of the ferric enzyme in this bifunctional protein.


Subject(s)
Chlorophenols/chemistry , Globins/chemistry , Hemoglobins/chemistry , Hydrogen Peroxide/chemistry , Oxygen/chemistry , Peroxidases/chemistry , Polychaeta/enzymology , Animals , Chromatography , Enzyme Activation , Halogens/chemistry , Heme/chemistry , Oxidation-Reduction
13.
J Inorg Biochem ; 104(3): 357-64, 2010 Mar.
Article in English | MEDLINE | ID: mdl-20110129

ABSTRACT

Nitric oxide synthase (NOS) catalyzes the NADPH- and O(2)-dependent oxidation of l-arginine (l-Arg) to nitric oxide (NO) and citrulline via an N(G)-hydroxy-l-arginine (NHA) intermediate. Mammalian NOSs have been studied quite extensively; other eukaryotes and some prokaryotes appear to express NOS-like proteins comparable to the oxygenase domain of mammalian NOSs. In this study, a recombinant NOS-like protein from the thermostable bacterium Geobacillus stearothermophilus (gsNOS) has been characterized using magnetic circular dichroism (MCD) and UV-Vis absorption spectroscopic techniques. Spectral comparisons of ligand complexes (with O(2), NO and CO) of substrate-bound (l-Arg or NHA) gsNOS, including the key oxyferrous complex studied at -50 degrees C in cryogenic mixed solvents, with analogous mammalian NOS complexes indicate overall spectroscopic similarities between gsNOS and mammalian NOSs. However, more detailed spectral comparisons reflect subtle structural differences between gsNOS and mammalian NOSs. This may be due to an incomplete tetrahydrobiopterin (BH(4))-binding site and low BH(4)-binding affinity, which may become even lower in the presence of cryosolvent in gsNOS. Although BH(4)-binding may be altered, gsNOS appears to require the pterin for NO production since formation of the stable ferric-NO product complex was only observed when excess BH(4) (>150muM) over gsNOS was present upon single turnover reaction in which O(2) was bubbled into dithionite-reduced NHA-bound protein solution at -35 degrees C or -50 degrees C.


Subject(s)
Bacterial Proteins/chemistry , Circular Dichroism/methods , Geobacillus stearothermophilus/enzymology , Nitric Oxide Synthase/chemistry , Animals , Bacterial Proteins/genetics , Bacterial Proteins/metabolism , Iron/chemistry , Iron/metabolism , Ligands , Nitric Oxide/biosynthesis , Nitric Oxide Synthase/genetics , Nitric Oxide Synthase/metabolism , Oxidation-Reduction , Oxygen/chemistry , Oxygen/metabolism , Recombinant Proteins/chemistry , Recombinant Proteins/genetics , Recombinant Proteins/metabolism , Spectrophotometry, Ultraviolet
14.
Arch Biochem Biophys ; 489(1-2): 68-75, 2009 Sep.
Article in English | MEDLINE | ID: mdl-19622342

ABSTRACT

The heme iron coordination of ferric myoglobin (Mb) in the presence of 9.0M urea and 8.0M acetic acid at acidic pH values has been probed by electronic absorption, magnetic circular dichroism and resonance Raman spectroscopic techniques. Unlike Mb at pH 2.0, where heme is not released from the protein despite the acid denaturation and the loss of the axial ligand, upon increasing the concentration of either urea or acetic acid, a spin state change is observed, and a novel, non-native six-coordinated high-spin species prevails, where heme is released from the protein.


Subject(s)
Acetic Acid/chemistry , Heme/chemistry , Iron/chemistry , Myoglobin/chemistry , Urea/chemistry , Animals , Cattle , Horses , Hydrogen-Ion Concentration , Molecular Structure , Protein Denaturation
15.
Dalton Trans ; (21): 3464-9, 2005 Nov 07.
Article in English | MEDLINE | ID: mdl-16234926

ABSTRACT

We recently used cryoreduction EPR/ENDOR techniques to show that a substrate can modulate the properties of both the monooxygenase active-oxygen intermediates and of the proton-delivery network which encompasses them. In the present report we use Q-band pulsed 19F ENDOR (Mims 3-pulse sequence) to examine the substrate binding geometries of camphor, through use of the 5,5'--difluorocamphor, and 13C ENDOR to examine the binding of 5-methylenyl camphor labeled with 13C at C11. These probes are examined in multiple states of the catalytic cycle of P450cam and its T252A mutant. As part of this investigation we further report a new cryoreduction reaction, the reduction of a ferroheme to the EPR-visible Fe(I) state, and use it to probe the substrate binding to the EPR-silent ferroheme state. Finally we report the solvent kinetic isotope effect on the decay of the camphor complex of the hydroperoxo-ferric intermediate, the first such measurement on an individual step within the P450cam reaction cycle. Following reduction of oxyferrous-P450cam, this step is the rate-limiting step in camphor hydroxylation, and its solv-KIE of 1.8 at 190 K establishes that it involves activation of the hydroperoxo moiety by transfer of the 'second' proton of catalysis. We suggest that the finding that the heme pocket can exist in multiple substates, including multiple substrate binding locations, even in P450cam, along with the established possibility that the hydroperoxo-ferriheme intermediate can react with substrate, may explain the formation of multiple products by P450s.


Subject(s)
Camphor 5-Monooxygenase/chemistry , Camphor 5-Monooxygenase/metabolism , Mutant Proteins/chemistry , Mutant Proteins/metabolism , Alanine/genetics , Alanine/metabolism , Camphor 5-Monooxygenase/genetics , Carbon Isotopes , Catalysis , Electron Spin Resonance Spectroscopy , Fluorine/analysis , Fluorine/chemistry , Heme/chemistry , Heme/metabolism , Iron/chemistry , Iron/metabolism , Kinetics , Models, Molecular , Mutant Proteins/genetics , Mutation/genetics , Oxidation-Reduction , Oxygen/chemistry , Oxygen/metabolism , Protein Binding , Protein Structure, Tertiary , Solvents/chemistry , Substrate Specificity , Threonine/genetics , Threonine/metabolism
16.
Biochem Biophys Res Commun ; 338(1): 365-71, 2005 Dec 09.
Article in English | MEDLINE | ID: mdl-16197919

ABSTRACT

We describe herein for the first time the formation and spectroscopic characterization of homogeneous oxyferrous complexes of the cytochrome P450 BM3 (CYP102) holoenzyme and heme domain (BMP) at -55 degrees C using a 70/30 (v/v) glycerol/buffer cryosolvent. The choice of buffer is a crucial factor with Tris [tris(hydroxymethyl)aminomethane] buffer being significantly more effective than phosphate. The oxyferrous complexes have been characterized with magnetic circular dichroism spectroscopy and the resulting spectra compared to those of the more well-characterized oxyferrous cytochrome P450-CAM. The formation of a stable substrate-bound oxyferrous CYP BM3 holoenzyme, despite the fact that it has the necessary reducing equivalents for turnover, indicates that electron transfer from the flavin domain to the oxyferrous center is very slow at this temperature. The ability to prepare stable homogeneous oxyferrous derivatives of both BMP and the CYP BM3 holoenzyme will enable these species to be used as starting materials for mechanistic investigation of dioxygen activation.


Subject(s)
Bacterial Proteins/chemistry , Cytochrome P-450 Enzyme System/chemistry , Freezing , Iron/metabolism , Mixed Function Oxygenases/chemistry , Oxygen/metabolism , Bacterial Proteins/metabolism , Catalytic Domain , Circular Dichroism , Cytochrome P-450 Enzyme System/metabolism , Enzyme Stability , Mixed Function Oxygenases/metabolism , NADPH-Ferrihemoprotein Reductase , Protein Structure, Tertiary , Recombinant Proteins/chemistry , Recombinant Proteins/metabolism , Spectrophotometry , Thermodynamics
17.
Arch Biochem Biophys ; 436(1): 40-9, 2005 Apr 01.
Article in English | MEDLINE | ID: mdl-15752707

ABSTRACT

To probe whether the nature of the substrate can directly influence the spectral properties of oxyferrous cytochrome P450-CAM, the complex has been investigated in the absence and in the presence of the natural substrate (1R)-camphor (camphor) and of several camphor analogs. The oxyferrous complex of T252A P450-CAM, a mutant lacking the hydroxyl group that forms a hydrogen bond to the heme iron-coordinated dioxygen, has also been studied to gauge the influence of this hydrogen bond. UV-visible absorption and magnetic circular dichroism (MCD) spectra of these oxyferrous adducts prepared and stabilized at -40 degrees C in 60% (v/v) ethylene glycol are generally similar, exhibiting absorption bands at approximately 355, approximately 420, approximately 554, and approximately 585 nm (shoulder) and a characteristic MCD trough at approximately 585 nm. The MCD spectrum of camphor-bound oxyferrous P450-CAM is similar to that of the substrate-free oxyferrous enzyme, but the spectrum of the oxyferrous enzyme differs detectably in the presence of substrate analogs. The spectra of the oxyferrous T252A mutant and wild-type enzyme are overall similar except for Soret band position blue shifts by 2-6 nm for the mutant. 5-Methylenylcamphor (epoxidation substrate) appears to have an anomalous binding mode for the mutant compared with that for the wild-type enzyme. The present results indicate that the structures of the camphor analogs can sensitively influence the physical (spectroscopic) properties of the P450 dioxygen complex and could also affect its reactivity. The ability of substrate to modulate the reactivity of P450 intermediates could be a relevant factor in explaining the remarkable diversity of reactions catalyzed by the enzyme.


Subject(s)
Cytochrome P-450 Enzyme System/metabolism , Ferrous Compounds/metabolism , Binding Sites , Camphor/analogs & derivatives , Camphor/metabolism , Camphor 5-Monooxygenase/chemistry , Camphor 5-Monooxygenase/metabolism , Catalysis , Cytochrome P-450 Enzyme System/chemistry , Ferrous Compounds/chemistry , Heme/chemistry , Heme/metabolism , Hydrogen Bonding , Models, Molecular , Mutation , Oxidation-Reduction , Oxygen/chemistry , Oxygen/metabolism , Spectrophotometry , Substrate Specificity
18.
J Am Chem Soc ; 127(5): 1403-13, 2005 Feb 09.
Article in English | MEDLINE | ID: mdl-15686372

ABSTRACT

EPR/ENDOR studies have been carried out on oxyferrous cytochrome P450cam one-electron cryoreduced by gamma-irradiation at 77 K in the absence of substrate and in the presence of a variety of substrates including its native hydroxylation substrate, camphor (a), and the alternate substrates, 5-methylenyl-camphor (b), 5,5-difluorocamphor (c), norcamphor (d), and adamantanone (e); the equivalent experiments have been performed on the T252A mutant complexed with a and b. The present study shows that the properties and reactivity of the oxyheme and of both the primary and the annealed intermediates are modulated by a bound substrate. This includes alterations in the properties of the heme center itself (g tensor; (14)N, (1)H, hyperfine couplings). It also includes dramatic changes in reactivity: the presence of any substrate increases the lifetime of hydroperoxoferri-P450cam (2) no less than ca. 20-fold. Among the substrates, b stands out as having an exceptionally strong influence on the properties and reactivity of the P450cam intermediates, especially in the T252A mutant. The intermediate, 2(T252A)-b, does not lose H(2)O(2), as occurs with 2(T252A)-a, but decays with formation of the epoxide of b. Thus, these observations show that substrate can modulate the properties of both the monoxygenase active-oxygen intermediates and the proton-delivery network that encompasses them.


Subject(s)
Adamantane/analogs & derivatives , Camphor 5-Monooxygenase/chemistry , Ferric Compounds/chemistry , Ferrous Compounds/chemistry , Adamantane/chemistry , Adamantane/metabolism , Binding Sites , Camphor/analogs & derivatives , Camphor/metabolism , Camphor 5-Monooxygenase/metabolism , Electron Spin Resonance Spectroscopy , Ferric Compounds/metabolism , Ferrous Compounds/metabolism , Hydroxylation , Models, Molecular , Recombinant Proteins/chemistry , Recombinant Proteins/metabolism , Substrate Specificity
19.
Proc Natl Acad Sci U S A ; 100(7): 3641-6, 2003 Apr 01.
Article in English | MEDLINE | ID: mdl-12655049

ABSTRACT

Cysteine plays a key role as a metal ligand in metalloproteins. In all well-recognized cases, however, it is the anionic cysteinate that coordinates. Several cysteinate-ligated heme proteins are known, but some fail to retain thiolate ligation in the ferrous state, possibly following protonation to form neutral cysteine. Ligation by cysteine thiol in ferrous heme proteins has not been documented. To establish spectroscopic signatures for such systems, we have prepared five-coordinate adducts of the ferrous myoglobin H94G cavity mutant with neutral thiol and thioether sulfur donors as well as six-coordinate derivatives such as with CO and, when possible, with NO and O(2). A thiol-ligated oxyferrous complex is reported, to our knowledge for the first time. Further, a bis-thioether ferrous H93G model for bis-methionine ligation, as found in Pseudomonas aeruginosa bacterioferritin heme protein, is described. Magnetic CD spectroscopy has been used due to its established ability in axial ligand identification. The magnetic CD spectra of the H93G complexes have been compared with those of ferrous H175CD235L cytochrome c peroxidase to show that its proximal ligand is neutral cysteine. We had previously reported this cytochrome c peroxidase mutant to be cysteinate-ligated in the ferric state, but the ferrous ligand was undetermined. The spectral properties of ferrous liver microsomal cytochrome P420 (inactive P450) are also consistent with thiol ligation. This study establishes that neutral cysteine can serve as a ligand in ferrous heme iron proteins, and that ferric cysteinate-ligated heme proteins that fail to retain such ligation on reduction may simply be ligated by neutral cysteine.


Subject(s)
Cysteine/chemistry , Hemeproteins/chemistry , Myoglobin/chemistry , Oxygen/chemistry , Sulfhydryl Compounds/chemistry , Circular Dichroism , Cytochrome-c Peroxidase/chemistry , Cytochrome-c Peroxidase/metabolism , Electron Transport Complex IV/chemistry , Electron Transport Complex IV/metabolism , Myoglobin/genetics , Oxidation-Reduction , Protein Binding , Protein Conformation , Spectrophotometry
20.
Biochemistry ; 42(8): 2475-84, 2003 Mar 04.
Article in English | MEDLINE | ID: mdl-12600215

ABSTRACT

Nitric oxide synthases (NOS) are a family of cysteine thiolate-ligated heme-containing monooxygenases that catalyze the NADPH-dependent two-step conversion of L-arginine to NO and L-citrulline. During the catalysis, a portion of the NOS heme forms an inhibitory complex with self-generated NO that is subsequently reverted back to NO-free active enzyme under aerobic conditions, suggesting a downstream regulator role of NO. Recent studies revealed that mutation of a conserved proximal tryptophan-409, which forms one of three hydrogen bonds to the heme-coordinated cysteine thiolate, to tyrosine or phenylalanine considerably increases the turnover number of neuronal NOS (nNOS). To further understand these properties of nNOS on its active site structural level, we have examined the oxygenase (heme-containing) domain of the two mutants in close comparison with that of wild-type nNOS with UV-visible absorption, magnetic circular dichroism, and electron paramagnetic resonance spectroscopy. Among several oxidation and ligation states examined, only the ferrous-NO adducts of the two mutants exhibit spectra that are markedly distinct from those of parallel derivatives of the wild-type protein. The spectra of the ferrous-NO mutants are broadly similar to those of known five-coordinate ferrous-NO heme complexes, suggesting that these mutants are predominantly five coordinate in their ferrous-NO states. The present results are indicative of cleavage of the Fe-S bond in the nNOS mutants in their ferrous-NO state and imply a significant role of the conserved tryptophan in stabilization of the Fe-S bond.


Subject(s)
Cysteine/chemistry , Ferrous Compounds/chemistry , Heme/chemistry , Iron-Sulfur Proteins/chemistry , Mutagenesis, Site-Directed , Nitric Oxide Synthase/chemistry , Nitric Oxide/chemistry , Circular Dichroism , Electron Spin Resonance Spectroscopy , Ferric Compounds/chemistry , Humans , Ligands , Nitric Oxide Synthase/genetics , Nitric Oxide Synthase Type I , Oxidation-Reduction , Phenylalanine/genetics , Protein Structure, Tertiary , Solvents , Spectrophotometry, Ultraviolet , Tryptophan/genetics , Tyrosine/genetics
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