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1.
Methods ; 55(4): 415-20, 2011 Dec.
Article in English | MEDLINE | ID: mdl-22079407

ABSTRACT

Studies on four membrane protein systems, which combine information derived from crystal structures and biophysical studies have emphasized, as a precursor to crystallization, demonstration of functional activity. These assays have relied on sensitive spectrophotometric, electrophysiological, and microbiological assays of activity to select purification procedures that lead to functional complexes and with greater likelihood to successful crystallization: (I), Hetero-oligomeric proteins involved in electron transport/proton translocation. (1) Crystal structures of the eight subunit hetero-oligomeric trans-membrane dimeric cytochrome b(6)f complex were obtained from cyanobacteria using a protocol that allowed an analysis of the structure and function of internal lipids at specific intra-membrane, intra-protein sites. Proteolysis and monomerization that inactivated the complex and prevented crystallization was minimized through the use of filamentous cyanobacterial strains that seem to have a different set of membrane-active proteases. (2) An NADPH-quinone oxido-reductase isolated from cyanobacteria contains an expanded set of 17 monotopic and polytopic hetero-subunits. (II) ß-Barrel outer membrane proteins (OMPs). High resolution structures of the vitamin B(12) binding protein, BtuB, solved in meso and in surfo, provide the best example of the differences in such structures that were anticipated in the first application of the lipid cubic phase to membrane proteins [1]. A structure of the complex of BtuB with the colicin E3 and E2 receptor binding domain established a "fishing pole" model for outer membrane receptor function in cellular import of nuclease colicins. (III) A modified faster purification procedure contributed to significantly improved resolution (1.83Å) of the universal porin, OmpF, the first membrane protein for which meaningful 3D crystals have been obtained [2]. A crystal structure of the N-terminal translocation domain of colicin E3 complexed to OmpF established the role of OmpF as an import channel for colicin nuclease cytotoxins. (IV) α-Synuclein, associated with the etiology of Parkinson's Disease, is an example of a protein, which is soluble and disordered in solution, but which can assume an ordered predominantly α-helical conformation upon binding to membranes. When subjected in its membrane-bound form to a trans-membrane electrical potential, α-synuclein can form voltage-gated ion channels. Summary of methods to assay functions/activities: (i) sensitive spectrophotometric assay to measure electron transfer activities; (ii) hydrophobic chromatography to deplete lipids, allowing reconstitution with specific lipids for studies on lipid-protein interactions; (iii) microbiological screen to assay high affinity binding of colicin receptor domains to Escherichia coli outer membrane receptors; (iv) electrophysiology/channel analysis (a) to select channel-occluding ligands for co-crystallization with ion channels of OmpF, and (b) to provide a unique description of voltage-gated ion channels of α-synuclein.


Subject(s)
Bacterial Outer Membrane Proteins/chemistry , Cytochrome b6f Complex/chemistry , Escherichia coli Proteins/chemistry , Membrane Transport Proteins/chemistry , NADPH Dehydrogenase/chemistry , Porins/chemistry , alpha-Synuclein/chemistry , Crystallization , Crystallography, X-Ray , Cyanobacteria/enzymology , Enzyme Assays , Escherichia coli/enzymology , Humans , Models, Molecular , NADPH Dehydrogenase/isolation & purification , Protein Structure, Secondary , Protein Structure, Tertiary , Protein Subunits/chemistry
2.
Biochemistry ; 40(50): 15109-16, 2001 Dec 18.
Article in English | MEDLINE | ID: mdl-11735393

ABSTRACT

Plastoquinol oxidation and proton transfer by the cytochrome b(6) f complex on the lumen side of the chloroplast thylakoid membrane are mediated by high and low potential electron transport chains. The rate constant for reduction, k(bred), of cytochrome b(6) in the low potential chain at ambient pH 7.5-8 was twice that, k(fred), of cytochrome f in the high potential chain, as previously reported. k(bred) and k(fred) have a similar pH dependence in the presence of nigericin/nonactin, decreasing by factors of 2.5 and 4, respectively, from pH 8 to an ambient pH = 6, close to the lumen pH under conditions of steady-state photosynthesis. A substantial kinetic isotope effect, k(H2O)/k(D2O), was found over the pH range 6-8 for the reduction of cytochromes b(6) and f, and for the electrochromic band shift associated with charge transfer across the b(6)f complex, showing that isotope exchange affects the pK values linked to rate-limiting steps of proton transfer. The kinetic isotope effect, k(bred)(H2O)/k(bred) (D2O) approximately 3, for reduction of cytochrome b in the low potential chain was approximately constant from pH 6-8. However, the isotope effect for reduction of cytochrome f in the high potential chain undergoes a pH-dependent transition below pH 6.5 and increased 2-fold in the physiological region of the lumen pH, pH 5.7-6.3, where k(fred)(H2O)/k(fred)(D2O) approximately 4. It is proposed that a rate-limiting step for proton transfer in the high potential chain resides in the conserved, buried, and extended water chain of cytochrome f, which provides the exit port for transfer of the second proton derived from p-side quinol oxidation and a "dielectric well" for charge balance.


Subject(s)
Cytochrome b Group/chemistry , Plastoquinone/analogs & derivatives , Chloroplasts/chemistry , Chloroplasts/metabolism , Cytochrome b Group/metabolism , Cytochrome b6f Complex , Deuterium , Electrochemistry , Electron Transport , Hydrogen-Ion Concentration , Models, Biological , Oxidation-Reduction , Plastoquinone/chemistry , Plastoquinone/metabolism , Protons , Spinacia oleracea , Thermodynamics
3.
Biochemistry ; 39(31): 9164-73, 2000 Aug 08.
Article in English | MEDLINE | ID: mdl-10924110

ABSTRACT

The structure of cytochrome f includes an internal chain of five water molecules and six hydrogen-bonding side chains, which are conserved throughout the phylogenetic range of photosynthetic organisms from higher plants, algae, and cyanobacteria. The in vivo electron transfer capability of Chlamydomonas reinhardtii cytochrome f was impaired in site-directed mutants of the conserved Asn and Gln residues that form hydrogen bonds with water molecules of the internal chain [Ponamarev, M. V., and Cramer, W. A. (1998) Biochemistry 37, 17199-17208]. The 251-residue extrinsic functional domain of C. reinhardtii cytochrome f was expressed in Escherichia coli without the 35 C-terminal residues of the intact cytochrome that contain the membrane anchor. Crystal structures were determined for the wild type and three "water chain" mutants (N168F, Q158L, and N153Q) having impaired photosynthetic and electron transfer function. The mutant cytochromes were produced, folded, and assembled heme at levels identical to that of the wild type in the E. coli expression system. N168F, which had a non-photosynthetic phenotype and was thus most affected by mutational substitution, also had the greatest structural perturbation with two water molecules (W4 and W5) displaced from the internal chain. Q158L, the photosynthetic mutant with the largest impairment of in vivo electron transfer, had a more weakly bound water at one position (W1). N153Q, a less impaired photosynthetic mutant, had an internal water chain with positions and hydrogen bonds identical to those of the wild type. The structure data imply that the waters of the internal chain, in addition to the surrounding protein, have a significant role in cytochrome f function.


Subject(s)
Chlamydomonas reinhardtii/enzymology , Cytochromes/chemistry , Photosynthesis , Water/chemistry , Animals , Asparagine/genetics , Brassica/enzymology , Chlamydomonas reinhardtii/genetics , Chlamydomonas reinhardtii/growth & development , Crystallography, X-Ray , Cyanobacteria/enzymology , Cytochromes/genetics , Cytochromes f , Glutamine/genetics , Hydrogen Bonding , Leucine/genetics , Molecular Sequence Data , Mutagenesis, Site-Directed , Oxidation-Reduction , Phenylalanine/genetics , Photosynthesis/genetics , Plant Proteins/chemistry , Protozoan Proteins/chemistry , Protozoan Proteins/genetics , Sequence Homology, Amino Acid , Spectrophotometry, Ultraviolet , Structure-Activity Relationship
4.
J Bioenerg Biomembr ; 31(3): 201-13, 1999 Jun.
Article in English | MEDLINE | ID: mdl-10591526

ABSTRACT

Structural alignment of the integral cytochrome b6-SU IV subunits with the solved structure of the mitochondrial bc1 complex shows a pronounced asymmetry. There is a much higher homology on the p-side of the membrane, suggesting a similarity in the mechanisms of intramembrane and interfacial electron and proton transfer on the p-side, but not necessarily on the n-side. Structural differences between the bc1 and b6f complexes appear to be larger the farther the domain or subunit is removed from the membrane core, with extreme differences between cytochromes c1 and f. A special role for the dimer may involve electron sharing between the two hemes b(p), which is indicated as a probable event by calculations of relative rate constants for intramonomer heme b(p) --> heme b(n), or intermonomer heme b(p) --> heme b(p) electron transfer. The long-standing observation of flash-induced oxidation of only approximately 0.5 of the chemical content of cyt f may be partly a consequence of the statistical population of ISP bound to cytfon the dimer. It is proposed that the p-side domain of cyt f is positioned with its long axis parallel to the membrane surface in order to: (i) allow its large and small domains to carry out the functions of cyt c1 and suVIII, respectively, of the bc1 complex, and (ii) provide maximum dielectric continuity with the membrane. (iii) This position would also allow the internal water chain ("proton wire") of cyt f to serve as the p-side exit port for an intramembrane H+ transfer chain that would deprotonate the semiquinol located in the myxothiazol/MOA-stilbene pocket near heme b(p). A hypothesis is presented for the identity of the amino acid residues in this chain.


Subject(s)
Cytochrome b Group/chemistry , Electron Transport Complex III/chemistry , Models, Molecular , Protein Conformation , Animals , Bacterial Proteins/chemistry , Cytochrome b6f Complex , Dicyclohexylcarbodiimide/pharmacology , Dimerization , Electron Transport , Heme/chemistry , Oxidation-Reduction , Protein Structure, Tertiary , Protons , Structure-Activity Relationship , Ubiquinone/chemistry
5.
Biofactors ; 9(2-4): 81-6, 1999.
Article in English | MEDLINE | ID: mdl-10416018

ABSTRACT

Cytochrome bc1, a multi-subunit integral membrane protein complex found in mammalian mitochondria, plays a central role in the transfer of electrons and protons generated by the oxidation of ubiquinol. According to the classical chemiosmotic theory, quinones shuttle protons across the hydrophobic membrane bilayer with the net result of H+ transfer to the aqueous side and generation of an electrochemical proton gradient. Recently, high-resolution structures of the mitochondrial bc1 complex showed quinone binding sites at one of the transmembrane helices of cytochrome b, and two potentially protonatable histidine residues on the Rieske iron-sulfur protein. The modern biochemical refinements of the original chemiosmotic theory require electron and proton transfer from quinones to particular residues/redox centers of integral membrane proteins and subsequent transfer of H+ to the bulk aqueous phase outside the membrane.


Subject(s)
Electron Transport Complex III/chemistry , Electron Transport Complex III/metabolism , Mitochondria/enzymology , Animals , Electron Transport , Energy Metabolism , Iron-Sulfur Proteins/chemistry , Iron-Sulfur Proteins/metabolism , Mammals , Models, Chemical , Models, Molecular , Protein Conformation , Protein Structure, Secondary , Ubiquinone/analogs & derivatives , Ubiquinone/metabolism
6.
Biochemistry ; 37(43): 15120-8, 1998 Oct 27.
Article in English | MEDLINE | ID: mdl-9790675

ABSTRACT

The prominent basic patch seen in the atomic structure of the lumen-side domain of turnip cytochrome f, consisting of Arg209 and Lys187, 58, 65, and 66, was proposed to be an electrostatically complementary docking site for its physiological electron acceptor, plastocyanin [Martinez, S. E., Huang, D., Szczepaniak, A., Cramer, W. A., and Smith, J. L. (1994) Structure 2, 95-105]. This proposal agrees with solution studies on the cytochrome f/plastocyanin electron-transfer reaction that showed a major contribution of electrostatic interactions to the docking, but not with studies on the oxidation rate of cyt f in vivo using mutants in which the basic patch of cyt f was neutralized. The apparent contradiction might be explained by an unknown electron acceptor protein for cyt f. However, (i) flash-induced oxidation of cyt f is absent in a PC-deficient mutant. (ii) Lys58, 65, and 66 in the large domain and Lys188 and 189 in the small domain are major contributors to the ionic strength dependence of the electron-transfer reaction in solution. Replacement of Lys58 and 65 by neutral residues and of Lys66 by the acidic residue Glu66 resulted in a >10-fold decrease in the rate of electron transfer in solution and complete loss of its ionic strength dependence. Replacement of Lys188 and Lys189 in the small domain of cyt f resulted in a 3-4-fold decrease in the second-order rate constant and a smaller dependence of the overall rate of electron transfer on ionic strength, corresponding to a loss of two positive charges. (iii) Acidification of the thylakoid lumen cannot explain the absence of electrostatic interactions. (iv) Changing the five lysines to acidic residues did not result in any significant retardation of the rate of cyt f oxidation in vivo. If the docking of cyt f and plastocyanin in vivo is mediated by basic residues of cyt f, they are different from those that mediate electron transfer in vitro or that are implicated by simulations of electrostatic interactions of the docking. Alternatively, docking of cyt f/PC in vivo is limited by spatial constraints or release of PC from P700 that precludes a rate-limiting mediation of the cyt f/PC reaction by specific electrostatic interactions. The cyt f/PC system in Chlamydomonas reinhardtii is the first electron-transfer couple for which the role of electrostatics in mediating the docking reaction has been studied both in vitro and in vivo.


Subject(s)
Cytochromes/metabolism , Plastocyanin/metabolism , Animals , Chlamydomonas reinhardtii/enzymology , Chloroplasts/enzymology , Cytochromes/chemistry , Cytochromes/genetics , Cytochromes f , Electron Transport , Hydrogen-Ion Concentration , Intracellular Membranes/enzymology , Kinetics , Models, Molecular , Mutagenesis, Site-Directed , Oxidation-Reduction , Plastocyanin/chemistry , Static Electricity
7.
Biophys J ; 73(6): 3265-76, 1997 Dec.
Article in English | MEDLINE | ID: mdl-9414237

ABSTRACT

In a complex of two electron-transfer proteins, their redox potentials can be shifted due to changes in the dielectric surroundings and the electrostatic potentials at each center caused by the charged residues of the partner. These effects are dependent on the geometry of the complex. Three different docking configurations (DCs) for intracomplex electron transfer between cytochrome f and plastocyanin were studied, defined by 1) close contact of the positively charged region of cytochrome f and the negatively charged regions of plastocyanin (DC1) and by (2, 3) close contact of the surface regions adjacent to the Fe and Cu redox centers (DC2 and DC3). The equilibrium energetics for electron transfer in DC1-DC3 are the same within approximately +/-0.1 kT. The lower reorganization energy for DC2 results in a slightly lower activation energy for this complex compared with DC1 and DC3. The long heme-copper distance (approximately 24 A) in the DC1 complex drastically decreases electronic coupling and makes this complex much less favorable for electron transfer than DC2 or DC3. DC1-like complexes can only serve as docking intermediates in the pathway toward formation of an electron-transfer-competent complex. Elimination of the four positive charges arising from the lysine residues in the positive patch of cytochrome f, as accomplished by mutagenesis, exerts a negligible effect (approximately 3 mV) on the redox potential difference between cyt f and PC.


Subject(s)
Cytochromes/chemistry , Cytochromes/metabolism , Plastocyanin/chemistry , Plastocyanin/metabolism , Biophysical Phenomena , Biophysics , Cytochromes/genetics , Cytochromes f , Electron Transport , Kinetics , Macromolecular Substances , Models, Molecular , Mutagenesis, Site-Directed , Oxidation-Reduction , Protein Conformation , Static Electricity , Thermodynamics
8.
Biochemistry ; 35(46): 14590-8, 1996 Nov 19.
Article in English | MEDLINE | ID: mdl-8931557

ABSTRACT

The prominent interdomain basic surface region seen in the high-resolution structure of the active lumen-side C-terminal fragment of turnip cytochrome f, containing the conserved Lys58,65,66 (large domain) and Lys187 (small domain), has been inferred from in vitro studies to be responsible for docking of its physiological oxidant, plastocyanin. The effect of the putative docking region of cyt f on its reactivity in vivo was tested by site-directed mutagenesis in Chlamydomonas reinhardtii. Three charge-neutralizing mutants were constructed involving: (i)the two lysines (Lys188Asn-Lys189Gln) in the small domain, (ii) the three lysines (Lys58Gln-Lys65Ser-Lys66Glu) in the large domain, and (iii) all five of these lysines spanning both domains. All mutants grew phototrophically. The mutants displayed a 20-30% increase in average generation time, and comparable decreases in rates of steady-state oxygen evolution and the slow (millisecond) electrochromic 515 nm band shift. The magnitude of the changes was greatest in the 5-fold Lys-minus mutant (Lys58Gln-Lys65Ser-Lys66Glu-Lys188Asn-Lys189G ln). The mutants showed a small increase (approximately 25%) in the t1/2, from 0.2 to 0.25 ms, of cyt f photooxidation, far less than anticipated (ca. 100-fold) from in vitro studies of the effect of high ionic strength on the cyt f-PC interaction. The t1/2 of cyt f dark reduction via the Rieske protein increased from 5-6 ms in the wild type to 11-12 ms in the 5-fold Lys-minus mutant. Cells grown phototrophically in the absence of Cu, where cyt c6 is the electron acceptor of cyt f, displayed net rates of cytochrome photooxidation that were slightly faster than those in the presence of Cu, which also decreased by a factor of < or = 25% in the Lys-minus mutants. It was concluded that (a) the net effect of electrostatic interaction between cytochrome f and its electron acceptor in vivo is much smaller than measured in vitro and is not rate-limiting. This may be a consequence of a relatively high ionic strength environment and the small diffusional space available for collision and docking in the internal thylakoid lumen of log phase C. reinhardtii. (b) The efficiency of electron transfer to cytochrome f from the Rieske protein is slightly impaired by the neutralization of the lysine-rich domain.


Subject(s)
Cytochromes/chemistry , Animals , Binding Sites , Chlamydomonas reinhardtii , Cytochromes/genetics , Cytochromes f , Electron Transport , Models, Molecular , Mutagenesis, Site-Directed , Oxidation-Reduction , Oxygen , Static Electricity , Structure-Activity Relationship
9.
Article in English | MEDLINE | ID: mdl-15012298

ABSTRACT

The cytochrome b6f complex functions in oxygenic photosynthetic membranes as the redox link between the photosynthetic reaction center complexes II and I and also functions in proton translocation. It is an ideal integral membrane protein complex in which to study structure and function because of the existence of a large amount of primary sequence data, purified complex, the emergence of structures, and the ability of flash kinetic spectroscopy to assay function in a readily accessible ms-100 mus time domain. The redox active polypeptides are cytochromes f and b6 (organelle encoded) and the Rieske iron-sulfur protein (nuclear encoded) in a mol wt = 210,000 dimeric complex that is believed to contain 22-24 transmembrane helices. The high resolution structure of the lumen-side domain of cytochrome f shows it to be an elongate (75 A long) mostly beta-strand, two-domain protein, with the N-terminal alpha-amino group as orthogonal heme ligand and an internal linear 11-A bound water chain. An unusual electron transfer event, the oxidant-induced reduction of a significant fraction of the p (lumen)-side cytochrome b heme by plastosemiquinone indicates that the electron transfer pathway in the b6f complex can be described by a version of the Q-cycle mechanism, originally proposed to describe similar processes in the mitochondrial and bacterial bc1 complexes.

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