ABSTRACT
Accumulation of oxidized proteins (evaluated by the levels of carbonyl and SH groups) in tissues of 2-24-month-old rats (spleen>myocardium>testicles>liver>skeletal muscles) has been demonstrated. Exogenous delta sleep-inducing peptide injected subcutaneously to rats of different age in a dose of 100 µg/kg by monthly 5-day courses protected proteins of the studied tissues from oxidation; its effect was tissue-specific. Delta sleep-inducing peptide exhibited a hypoglycemic effect: it prevented nonenzymatic glycosylation of hemoglobin and reduced the level of defective protein molecules during aging.
Subject(s)
Aging/metabolism , Delta Sleep-Inducing Peptide/pharmacology , Animals , Liver/drug effects , Liver/metabolism , Male , Muscle, Skeletal/drug effects , Muscle, Skeletal/metabolism , Myocardium/metabolism , Oxidation-Reduction/drug effects , Rats , Spleen/drug effects , Spleen/metabolism , Testis/drug effects , Testis/metabolismABSTRACT
Certain carbohydrates and polyols are used at various stages of the production of immunobiological preparations as stabilizers of biological activity, particularly in the production of lactoglobulin (against opportunistic pathogens) using membrane ultrafiltration. This study concerns the effect of these substances on changes in the amide content in proteins of this lactoglobulin. Lactoglobulin was incubated in near-physiological (0.9% NaCl, pH 5.5) 10% solutions of glucose, fructose, and sorbitol at 4 and 35 degrees C for 7, 14, and 28 days. A lactoglobulin solution in 0.9% NaCl, pH 5.5, was used as the control. All substances studied suppressed the reduction of the amide group content of asparagine and, in contrast, increased the rate of amide group removal from glutamine residues in proteins of lactoglobulin preparations.
Subject(s)
Amides/chemistry , Hydrocarbons/chemistry , Lactoglobulins/chemistry , Polymers/chemistry , Hydrogen-Ion Concentration , Immunologic Factors/chemistryABSTRACT
Spontaneous deamidation of lactoferrin preparations from women's milk was found during incubation for 28 days under simulated physiological conditions (0.85% NaCl, pH 7.0, 37 degrees C). After 28 days of incubation, this deamidation was associated with a 12% decrease in the total amide content in the protein. Addition of deamidated preparation to a suspension of lipoproteins from egg yolk in the presence of Rhodamine 6G decreased the total intensity of rapid and slow emission and also the intensity of the slow emission of Fe2+-induced chemiluminescence by 37, 48, and 53%, respectively, suggesting an increase in the antioxidative activity of lactoferrin during deamidation. Deamidation obviously stimulated the nonspecific interaction of lactoferrin with iron ions and, consequently, increased the antioxidant effect of the protein in this way. This was supported by the finding of decreased antioxidative effectiveness of lactoferrin during its complete saturation with iron under the incubation conditions.
Subject(s)
Antioxidants/chemistry , Lactoferrin/chemistry , Milk, Human/metabolism , Animals , Antioxidants/metabolism , Female , Humans , Lactoferrin/metabolism , Luminescent Measurements , Milk, Human/chemistry , Oxidation-ReductionABSTRACT
The paper focuses on the membrane lipid complex status and lymphocyte metabolic activity in IHD patients to elaborate new approaches toward treatment and prevention of immune disorders in the above pathology. The study was made in blood specimens from 53 coronary patients presenting with progressive unstable angina. The diagnosis was made on the basis of a complex clinical-and-laboratory and electrocardiographic evaluation. The composition of the lymphocyte membrane lipid complex and blood serum was studied with the aid of the gas chromatography techniques. Three variants were identified of change in the lipid composition of lymphocyte membranes in IHD patients having undergone the above evaluation. We consider it expediant to use criteria for the state of lymphocyte membranes in IHD patients in order that we might properly deal with issues concerning diagnosis, prognosis and selection of optimum pathogenetic therapy of the medical condition in question.
Subject(s)
Lymphocytes/metabolism , Myocardial Ischemia/blood , Adult , Antioxidants/metabolism , Cell Membrane/metabolism , DNA/metabolism , Humans , Membrane Lipids/metabolism , Middle Aged , Myocardial Ischemia/metabolism , RNA/metabolismABSTRACT
To examine the impact of low-intensive laser radiation (LILR) on endocrine function in patients with pulmonary tuberculosis, the time course of blood levels of thyroid hormones, cortisol, and insulin was studied in 117 patients of whom 64 received complex treatment by using LILR. Pulmonary tuberculosis was ascertained to be characterized by a marked hormonal imbalance, including impaired metabolism of thyroid hormones, hypercorticism, altered pancreatic incretion, which negatively affects the efficiency of antituberculosis therapy. Intravenous and epicutaneous therapy promotes recovery of endocrine responsiveness and enhances the efficiency of treatment in patients with pulmonary tuberculosis.
Subject(s)
Endocrine Glands/radiation effects , Laser Therapy , Tuberculosis, Pulmonary/radiotherapy , Antitubercular Agents/therapeutic use , Combined Modality Therapy , Humans , Hydrocortisone/blood , Insulin/blood , Thyrotropin/blood , Thyroxine/blood , Time Factors , Triiodothyronine/blood , Tuberculosis, Pulmonary/blood , Tuberculosis, Pulmonary/drug therapyABSTRACT
Low-intensive laser radiation used by topical, intravenous, and transendoscopic routes was employed in the complex antituberculosis therapy of 120 patients with pulmonary tuberculosis concurrent with chronic nonspecific respiratory diseases (n = 48), diabetes mellitus (n = 20 patients), gastrointestinal diseases (n = 45). The results of treatment were compared with the parameters of 62 patients with the similar clinical and X-ray characteristics of a tuberculosis process and concomitant diseases. It was ascertained that laser therapy might enhance the efficiency of treatment of patients with concurrent abnormality statistically significantly, by reducing the time of bacterial isolation, closure of decay cavities in the lung tissue, hospital treatment and by accelerating the onset of stable remission of concomitant diseases.
Subject(s)
Diabetes Complications , Gastrointestinal Diseases/complications , Laser Therapy , Respiratory Tract Diseases/complications , Tuberculosis, Pulmonary/radiotherapy , Adolescent , Adult , Chronic Disease , Humans , Middle Aged , Treatment Outcome , Tuberculosis, Pulmonary/complicationsSubject(s)
Leukemia, Myelomonocytic, Acute/complications , Opportunistic Infections/etiology , Tuberculosis, Pulmonary/etiology , Acute Disease , Adolescent , Diagnosis, Differential , Fatal Outcome , Humans , Leukemia, Myelomonocytic, Acute/diagnosis , Male , Opportunistic Infections/diagnosis , Tuberculosis, Pulmonary/diagnosisABSTRACT
The influence of low-intensity garnet laser radiation a wavelength of 0.53 microns (green spectrum) on Mycobacterium tuberculosis (MT), nonspecific microflora and regeneration of aseptic and purulent wounds has been studied experimentally for the first time. Irradiation of MT and nonspecific microflora inoculations has shown that this type of laser radiation fails to stimulate growth of MT and nonspecific microflora and has an inhibiting action on MT manifested by delayed MT growth, a lower number of dimensions of the growth colonies as compared to those of the control inoculations and has a bacteriostatic action on micrococci and Neisseria organisms. Influence of this type of laser on the aseptic and purulent wounds in 50 guinea pigs has revealed a more rapid regeneration of aseptic wounds (by 3-4 days) as compared to the control.
