Carex pulicaris abundance is positively associated with soil acidity, rainfall and floristic diversity in the eastern distribution range.

Carex pulicaris is considered an endangered species, and further losses are forecast under the influence of even moderate climate change. Local studies indicate that temporal declines in C. pulicaris abundance are positively correlated to decreases in precipitation and increases in air temperature. Determining ecological properties on larger scales than local ones can help develop effective protection programs for the species. We hypothesize that the local relationships observed between C. pulicaris abundance and precipitation, air temperature and soil properties will be confirmed in a spatially-oriented large-scale study performed in situ. Therefore, the present study takes a novel, large-scale integrated approach to (1) precisely characterize the ecological requirements of C. pulicaris within its eastern distribution range, and (2) determine the influence of its community type, soil properties and climatic conditions on its abundance. It was found that C. pulicaris is not a dominant or codominant species in the studied phytocoenoses in the eastern distribution range. Five natural vegetation groups including C. pulicaris, with significantly diverse species compositions, were resolved: well supported Estonian, Polish, Slovak and Radecz groups, and a weakly-supported Ambiguous group. The abundance of C. pulicaris was found to be positively correlated with the composition of the geographically-diversified plant communities and atmospheric precipitation, and to be also negatively associated with latitude and soil pH. Although the species is adapted to a relatively wide range of soil types, such adaptation requires appropriate substrate moisture level and light conditions. The species prefers moist organic and mineral soils and grows on both acid and neutral medium, characterized by a narrow C:N ratio, with various amounts of digestible total P, Mg and N, and low levels of digestible K. Climate change, manifested by reduced rainfall, may be one of the most important predictors negatively affecting the occurrence of C. pulicaris.

Bioaccumulation of Macronutrients in Edible Mushrooms in Various Habitat Conditions of NW Poland-Role in the Human Diet.

Recently, the interest in mushroom consumption has been growing, since their taste and low calorific value are appreciated, but also due to their nutritional value. In determining the usefulness of mushrooms in the human diet, it is important to consider the conditions of their occurrence to perform the assessment of bioaccumulation of minerals. The aim of the study was: (a) to determine the content of selected macronutrients (P, K, Ca, Mg, Na) in fruiting bodies of Boletus edulis, Imleria badia, Leccinum scabrum and the soils, (b) to determine their bioaccumulation potential taking into account the habitat conditions, and (c) an attempt to estimate their role in covering the requirement for macronutrients of the human organism. The research material was obtained in the NW of Poland: Uznam and Wolin, the Drawa Plain and the Insko Lakeland. In the soil, we determined the content of organic matter, pH, salinity and the content of absorbable and general forms of macronutrients. The content of macronutrients in mushrooms was also determined. Chemical analyses were performed using the generally accepted test methods. The study showed that in NW Poland, B. edulis grew on the acidic soils of Arenosols, and I. badia and L. scabrum grew on Podzols. The uptake of K, Mg and Ca by the tested mushrooms was positively, and P and Na negatively correlated with the content of these elements in the soil. The acidity of the soil affected the uptake of K and Mg by mushrooms. There was no effect of the amount of organic matter in the soil noticed on the content of macronutrients (except sodium) in mushrooms. Among the studied macronutrients, none of the mushrooms accumulated Ca, while P and K were generally accumulated in the highest amounts, regardless of the species. Each of the other elements was usually accumulated at a similar level in the fruiting bodies of the species we studied. The exception was I. badia, which accumulated higher amounts of Mg compared to B. edulis and L. scabrum. Mushrooms can enrich the diet with some macronutrients, especially in P and K.

Morphological variability and genetic diversity in Carex buxbaumii and Carex hartmaniorum (Cyperaceae) populations.

BACKGROUND: Carex buxbaumii and C. hartmaniorum are sister species of the clade Papilliferae within the monophyletic section Racemosae. An unambiguous identification of these species is relatively difficult due to the interspecific continuum of some morphological characters as well as the intraspecific variability. The study was aimed at determining the range of variability, both morphological and genetic, within and between these two closely related and similar species. METHODS: The sedges were collected during botanical expeditions to Armenia, Estonia, the Netherlands, and Poland. The morphological separation of the two species and their populations was tested using the Discriminant Function Analysis (DFA). The genetic variability of the 19 Carex populations was assessed in the presence of eight Inter Simple Sequence Repeat (ISSR) primers. RESULTS: Results of the study indicate a considerable genetic affinity between the two sedge species (mean Si = 0.619). However, the populations of C. hartmaniorum are, morphologically and genetically, more homogenous than the populations of C. buxbaumii. Compared to C. hartmaniorum, C. buxbaumii usually has wider leaf blades, a shorter inflorescence, a lower number of spikes which are shorter, but wider, and longer bracts and utricles. The AMOVA showed a larger variation between the populations of C. buxbaumii, representing 25.65% of the total variation in the taxon. Two populations of C. buxbaumii (from Poland and Estonia) are separated from the remaining populations, both genetically and morphologically; their individuals show shorter utricles and glumes, compared to the typical specimens of C. buxbaumii, and correspond with the morphology of putative infraspecific taxa described by Cajander (var. brevisquamosa and var. confusa). CONCLUSIONS: The taxonomic status of the putative infraspecific taxa within C. buxbaumii requires further studies throughout the distribution range of C. buxbaumii, addressing habitats, morphology and genetics (including a chromosome count or a combination of different genetic methods), particularly as the variability in C. buxbaumii may be associated with the species' polyploid origin.

Phytochemical parasite-host relations and interactions: A Cistanche armena case study.

The holoparasitic genus Cistanche (Orobanchaceae) has been the most widely used and well known genus in traditional Chinese medicine for centuries. This is the first study that reports the phytochemical profile of Cistanche armena - an endemic species from Armenia and evaluates the composition and biological activity in relation to specific organs of the parasite (flowers vs stem with tuber) and its interaction with two host species: Alhagi maurorum (Fabaceae) and Salsola dendroides (Chenopodiaceae). We identified polyphenolic compounds using the UPLC-PDA-MS/MS method and quantified the antioxidative effects; inhibitory activities; polyphenol, nitrate III and nitrate V contents; ABTS+, DPPH, and FRAP activities; and colour parameters. A total of 28 polyphenolic compounds were tentatively identified. In C. armena, 9 compounds belonged to the phenylethanoid glycosides, mainly acteoside, B-hydroxyverbascoside and echinacoside, and in its hosts, 19 compounds belonged mainly to hydroxycinnamic acid and the flavanols. The profile of polyphenols in the host species was qualitatively and quantitatively different than the profile of the compounds in the parasite; this indicates the existence of a unique pathway of compound biosynthesis in the parasite. The colour and the amount and bioactivity of the polyphenolic compounds found in Cistanche were very diverse and depended on both the host plant and their location (organs) in the parasite. The stem and tuber of Cistanche hosted by Salsola had the highest polyphenol content, which was approximately 4 times higher than that in the stem and flowers of Cistanche individuals that parasitized A. maurorum. In addition, the stem and tuber of Cistanche that parasitized S. dendroides was characterized by the highest antioxidant activity (ABTS+, DPPH and FRAP) and high inhibitory activities. Conversely, the amount of polyphenols in the host Alhagi was 12 times higher than that in S. dendroides. These results highlight the importance of C. armena as a promising source of functional and bioactive ingredients (harvested from potential cultivation, not from natural endangered localities) and also draws the attention of future researchers to an important aspect regarding the parasite organ and the host's influence on the harvested material of various parasitic herbs.

Simple yet effective: Historical proximity variables improve the species distribution models for invasive giant hogweed (Heracleum mantegazzianum s.l.) in Poland.

Species distribution models are scarcely applicable to invasive species because of their breaking of the models' assumptions. So far, few mechanistic, semi-mechanistic or statistical solutions like dispersal constraints or propagule limitation have been applied. We evaluated a novel quasi-semi-mechanistic approach for regional scale models, using historical proximity variables (HPV) representing a state of the population in a given moment in the past. Our aim was to test the effects of addition of HPV sets of different minimal recentness, information capacity and the total number of variables on the quality of the species distribution model for Heracleum mantegazzianum on 116000 km2 in Poland. As environmental predictors, we used fragments of 103 1×1 km, world- wide, free-access rasters from WorldGrids.org. Single and ensemble models were computed using BIOMOD2 package 3.1.47 working in R environment 3.1.0. The addition of HPV improved the quality of single and ensemble models from poor to good and excellent. The quality was the highest for the variants with HPVs based on the distance from the most recent past occurrences. It was mostly affected by the algorithm type, but all HPV traits (minimal recentness, information capacity, model type or the number of the time periods) were significantly important determinants. The addition of HPVs improved the quality of current projections, raising the occurrence probability in regions where the species had occurred before. We conclude that HPV addition enables semi-realistic estimation of the rate of spread and can be applied to the short-term forecasting of invasive or declining species, which also break equal-dispersal probability assumptions.