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1.
J Proteome Res ; 8(1): 256-67, 2009 Jan.
Article in English | MEDLINE | ID: mdl-19032026

ABSTRACT

Several toxicological and environmental problems are associated with the extensive use of agricultural pesticides, such as herbicides. Nevertheless, little is known about the toxic effects of formulated herbicides, since many studies have been carried out using pure active molecules alone. In this work, we used as an eukaryotic model system an autochthonous wine yeast strain to investigate the effects of three commercial herbicides, currently used in the same geographical area from where this strain had been isolated. We carried out a comparative proteomic analysis to study the effects at the protein level of the herbicide-related stress, and found that the herbicides tested can alter the yeast proteome producing responses that share homologies with those observed treating yeast cells with the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) or with well-known oxidizing agents. We evaluated, through redox-proteomic techniques, protein carbonylation as a biomarker of oxidative stress. This analysis showed that herbicide-induced carbonylation is a dynamic phenomenon with degrees of selectivity.


Subject(s)
Herbicides/pharmacology , Oxidation-Reduction , Proteomics/methods , Saccharomyces cerevisiae/metabolism , 2,4-Dichlorophenoxyacetic Acid/chemistry , Carbon/chemistry , Electrophoresis, Gel, Two-Dimensional , Ethanol/chemistry , Fungal Proteins/chemistry , Isoelectric Focusing/methods , Models, Biological , Oxidative Stress , Oxygen/chemistry , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization , Wine
2.
J Agric Food Chem ; 54(8): 3163-72, 2006 Apr 19.
Article in English | MEDLINE | ID: mdl-16608247

ABSTRACT

Herbicides are released to the environment with potential ecotoxicological risks for mammals. Yeast is a good model to elucidate toxicity mechanisms. We investigated how three commercial herbicides (Proper Energy, Pointer, and Silglif) and their active ingredients (respectively, fenoxaprop-P-ethyl, tribenuron methyl, and glyphosate) can affect biological activities of an oenological Saccharomyces cerevisiae strain, which may be resident on grape vineyards of the same geographical areas where herbicides are used. The use of commercial grade herbicides employed in Italy allowed us to reproduce the same conditions applied in crops; at the same time, assaying pure single active compounds made it possible to compare the effects obtained with commercial formulations. Interestingly, we found that while pure active compounds affect cell growth and metabolism at a lower extent, commercial preparations have a significant major negative influence on yeast biology.


Subject(s)
Herbicides/chemistry , Herbicides/pharmacology , Saccharomyces cerevisiae/drug effects , Wine/microbiology , Fermentation , Saccharomyces cerevisiae/growth & development , Saccharomyces cerevisiae/metabolism
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