Subject(s)
Drug Eruptions/immunology , Lorazepam/immunology , Aged , Benzodiazepines/immunology , Humans , MaleABSTRACT
No disponible
Subject(s)
Humans , Male , Aged , Drug Hypersensitivity/immunology , Lorazepam/adverse effects , Exanthema/chemically induced , Drug-Related Side Effects and Adverse Reactions/immunology , Benzodiazepines/adverse effects , Pruritus/etiologyABSTRACT
Several studies have demonstrated different cross-reactivity patterns between proton pump inhibitors (PPIs). The aim of this study was to investigate cross-reactivity between commercially available PPIs and establish a procedure for performing cutaneous tests for verifying PPI allergy. We performed skin prick and intradermal tests with all commercially available PPIs in 5 patients with clinical allergy to omeprazole and observed positive results in all patients. We report 5 cases of immunoglobulin (Ig) E-mediated allergy to omeprazole and document cross-reactivity by skin testing between all the PPIs studied. We also found that the probability of confirming an IgE-mediated mechanism with skin tests decreases with time. Finally, we propose a hypothesis that could explain the different PPI cross-reactivity patterns reported.
Subject(s)
Drug Hypersensitivity/immunology , Hypersensitivity, Immediate/immunology , Omeprazole/immunology , Proton Pump Inhibitors/immunology , Adult , Angioedema , Cross Reactions , Drug Hypersensitivity/diagnosis , Drug Hypersensitivity/etiology , Drug Hypersensitivity/physiopathology , Female , Humans , Hypersensitivity, Immediate/chemically induced , Hypersensitivity, Immediate/diagnosis , Hypersensitivity, Immediate/physiopathology , Male , Middle Aged , Models, Immunological , Omeprazole/adverse effects , Omeprazole/chemistry , Proton Pump Inhibitors/adverse effects , Proton Pump Inhibitors/chemistry , Skin Tests , UrticariaABSTRACT
No disponible
Subject(s)
Infant , Male , Humans , Dietary Proteins/adverse effects , Vomiting/etiology , Oryza/adverse effects , Allergens , Skin TestsABSTRACT
No disponible
Subject(s)
Adult , Male , Humans , Pulmonary Eosinophilia/diagnosis , Churg-Strauss Syndrome/diagnosis , Radiography, Thoracic , Bronchoscopy , Diagnosis, Differential , Immunoglobulin E/blood , Respiratory Function TestsABSTRACT
La sensibilización al fluido seminal humano (FSH) es una rara causa de anafilaxia. Se presenta un caso de anafilaxia por sensibilización al FSH en una mujer de 48 años con dos hijos y antecedentes de urticaria-angioedema tras la ingesta de crustáceos. Su marido había sido vasectomizado tres años antes. Treinta minutos después del coito presentó urticaria, prurito, dolor genital y disnea. Se demostró un mecanismo de hipersensibilidad tipo I mediante la realización de prick con FSH que fue positivo, determinación de IgE específica clase 1 (0,35 KU/l) e isoelectroenfoque-immunoblotting con fijación de IgE entre punto isoeléctrico (pI) 5,67-6,89 (AU)
Subject(s)
Female , Middle Aged , Humans , Anaphylaxis/etiology , Semen , Hypersensitivity, Immediate/etiology , Angioedema/etiology , Immunologic Tests/methodsABSTRACT
Desde la introducción de los anestésicos locales del grupo amida y a pesar de su uso, son pocos los casos descritos de sensibilización de contacto a este grupo. Al contrario, los anestésicos del grupo éster se consideran frecuentes sensibilizantes. Se presenta un caso de sensibilización a lidocaína en una paciente que tolera otro anestésico del grupo amida, como la articaína. Se realizaron test epicutáneos con estándar europeo, batería de colirios y esparadrapo de tela y papel con resultado negativo; asimismo, se realizaron pruebas epicutáneas con anestésicos locales del grupo amida y del grupo éster, resultando positivas a lidocaína, mepivacaína y bupivacaína, y negativas a articaína y a anestésicos locales del grupo éster. También se realizaron pruebas en intradermorreacción presentando reacción con lidocaína y mepivacaína, tolerando articaína y procaína (AU)
Subject(s)
Aged , Female , Humans , Lidocaine/adverse effects , Drug Hypersensitivity/immunology , Lens Implantation, Intraocular , Anesthetics, Local/adverse effects , Skin Tests/methods , Intradermal Tests/methodsABSTRACT
No disponible
Subject(s)
Adult , Male , Humans , Contrast Media/adverse effects , Hypersensitivity, Delayed/immunology , Drug Hypersensitivity/drug therapy , Adrenal Cortex Hormones/therapeutic use , Histamine H1 Antagonists/therapeutic use , Immunologic TestsABSTRACT
No disponible
Subject(s)
Adult , Aged , Female , Male , Middle Aged , Humans , Dermatitis, Allergic Contact/immunology , Metals/adverse effects , Dental Implants/adverse effects , Vanadium/adverse effects , Nickel/adverse effects , Palladium/adverse effects , Cadmium/adverse effects , Skin Tests/methodsABSTRACT
Fundamento: Las dermatitis de contacto por textiles se producen fundamentalmente por sensibilización a los productos de acabado o a los tintes utilizados sobre las fibras textiles. El objetivo de este estudio es valorar la importancia de esta enfermedad en nuestro medio, cuáles son los antígenos más frecuentemente implicados y cómo están éstos representados en la serie estándar. Métodos: Se estudiaron 18 pacientes en los que se sospechó la existencia de dermatitis de contacto por textiles. Se efectuaron pruebas epicutáneas con la batería estándar y con una batería de contactantes textiles. Resultados: El 88 por ciento de los 18 pacientes recogidos presentaron lesiones en zonas típicas (cuello, axilas, muslos, zonas de mayor fricción y sudación) y el 72 por ciento las relacionaba con el uso de prendas textiles. Sólo tres pacientes presentaron sensibilización frente a PPD y dos al formaldehído. Los alergenos textiles más frecuentemente implicados fueron los tintes tipo disperse, sobre todo los pertenecientes al grupo azo (Disperse blue-124, Disperse blue-106, Disperse orange-1 y Disperse yellow-3). Tres pacientes presentaron sensibilización de contacto a resinas de formaldehído (Kaurit y Fix). Conclusiones: La dermatitis de contacto por textiles está probablemente infravalorada, debido a que no siempre se presenta de forma típica y a que carecemos de un marcador adecuado en la serie estándar. (AU)
Subject(s)
Adolescent , Adult , Female , Male , Middle Aged , Humans , Dermatitis, Allergic Contact/etiology , Coloring Agents/adverse effects , Azo Compounds/adverse effects , Formaldehyde/adverse effects , Skin Tests/methods , Perfume/adverse effectsABSTRACT
We report the case of a woman who developed generalized dermatitis after 1 week of treatment with ebrotidine, a new H2-receptor antagonist taken to prevent gastroduodenal lesions caused by nonsteroidal inflammatory drugs. Patch tests with ebrotidine and other H2-receptor antagonists ranitidine, cimetidine and famotidine were negative. Oral challenge test with ebrotidine showed the development of lesions similar to those appearing previously. Oral challenge test with ranitidine and cimetidine were negative, possibly due to the difference in the side chain chemical structure of ebrotidine and other H2-receptor antagonists. This is the first reported case of allergic dermatitis caused by ebrotidine.
Subject(s)
Benzenesulfonates/adverse effects , Dermatitis/etiology , Drug Eruptions/etiology , Drug Hypersensitivity/etiology , Thiazoles/adverse effects , Administration, Oral , Anti-Ulcer Agents/adverse effects , Benzenesulfonates/administration & dosage , Female , Histamine H2 Antagonists/adverse effects , Humans , Middle Aged , Thiazoles/administration & dosageABSTRACT
Monocytes play a key role in inflammation, tissue injury and remodelling and wound healing, and most monocyte effector functions are dependent on adhesive interactions. We have analyzed the changes in the pattern of beta 1 integrin expression that take place during monocyte activation and demonstrated that lipopolysaccharide (LPS) and interferon (IFN)-gamma specifically induce the expression of the alpha 1/beta 1 integrin, which was detectable on the monocyte membrane as early as 12 h after monocyte activation. The up-regulated alpha 1/beta 1 expression was not dependent on monocyte adherence to solid surfaces, and Northern blot analysis revealed that LPS and IFN-gamma induce the alpha 1 mRNA de novo. Monocyte deactivating cytokines such as interleukin (IL)-4 or IL-10, could only minimally inhibit the LPS- or IFN-gamma mediated up-regulation of alpha 1/beta 1, suggesting that cytokine release subsequent to monocyte activation does not play a major role in the integrin induction. Interestingly, the LPS-induced expression of alpha 1/beta 1 was found to be dependent on the redox state of the cell, since it was inhibited by antioxidants which also altered the morphological changes that take place during monocyte culture in vitro. The rapid induction of alpha 1 in LPS-activated monocytes suggests that alpha 1/beta 1 might be involved not only in monocyte/extracellular matrix interactions during inflammatory reactions, but also in contributing to further monocyte activation and cytokine production during septic shock syndrome.
Subject(s)
Integrins/biosynthesis , Interferon-gamma/pharmacology , Lipopolysaccharides/pharmacology , Monocytes/metabolism , Cell Adhesion , Cells, Cultured , Humans , Integrin alpha1beta1 , Monocytes/immunology , Oxidation-Reduction , Up-RegulationABSTRACT
To study the influence of the cellular environment on the functional activity of leukocyte integrins and to analyze their involvement in hematopoietic cell differentiation, we have developed stable transfectants of LFA-1, Mac-1, and p150,95 (CD11a-c/CD18) leukocyte integrins in cultured cell lines whose differentiation can be induced in vitro. As on circulating leukocytes, the integrins expressed on U937 or K562 cells were expressed in a constitutively inactive state, as demonstrated by the lack of adhesion to their cellular counterreceptors or soluble ligands, the absence of CD18-dependent intercellular aggregation, and their inability to mediate adhesion to protein-coated plates. However, while leukocyte integrin adhesive functions in U937 cells were induced upon treatment with cellular agonists (e.g., PMA), their function in K562 cells could be upregulated only with activating monoclonal antibodies, demonstrating the cell-type-specific regulation of the adhesive capabilities of the three leukocyte integrins in hematopoietic cellular environment. On the other hand, the expression of either CD11b/CD18 or CD11c/CD18 in U937 myeloid cells before induction of differentiation greatly affected the adhesive phenotype of differentiating cells by abrogating the CD11a/CD18-CD54-dependent homotypic aggregation. Unlike that of mock-transfected U937 cells, differentiation of CD11b/CD18- or CD11c/CD18-transfected U937 cells led to cell adhesion and spreading on the tissue culture plates, with an almost total absence of homotypic aggregates. These results confirm the role of CD11b/CD18 and CD11c/CD18 in myeloid cell adhesion and spreading and suggest that the CD11b/- and CD11c/CD18-mediated recognition of substrate-bound ligands competes or interferes with LFA-1-dependent intercellular adhesion.
Subject(s)
Hematopoietic Stem Cells/physiology , Integrin alphaXbeta2/metabolism , Integrins/metabolism , Lymphocyte Function-Associated Antigen-1/metabolism , Macrophage-1 Antigen/metabolism , Cell Adhesion , Cell Aggregation , Cell Differentiation , Cell Line , Humans , In Vitro Techniques , Leukemia, Erythroblastic, Acute/pathologyABSTRACT
Mukali (Aningeria robusta) is an exotic wood from the Sapotaceae family. We describe the case of a 31-year-old woman who developed bronchial asthma and contact urticaria after occupational exposure to mukali. A positive immediate response was observed in the skin prick test, bronchial challenge and the rubbing test performed with a mukali wood extract. We suggest that an IgE-mediated immunological mechanism could explain these findings. Our case report identifies another wood dust capable of inducing occupational asthma.
Subject(s)
Asthma/etiology , Dust , Occupational Diseases/etiology , Urticaria/etiology , Wood , Adult , Female , Humans , Industry , Interior Design and FurnishingsABSTRACT
We report on a patient with asthma induced by occupational exposure to egg used to spray cakes before baking. A type I hypersensitivity to egg white was demonstrated by means of skin test, immunoassay for specific IgE, and immediate bronchial provocation test response to an egg white extract.
Subject(s)
Asthma/etiology , Egg White/adverse effects , Food Hypersensitivity/etiology , Occupational Diseases/etiology , Adult , Humans , MaleABSTRACT
We report the case of a grain worker who had immediate nasal and conjunctival symptoms and late-phase asthmatic reactions after handling stored grain. Allergy to one storage mite, Lepidoglyphus destructor, was demonstrated by skin testing, specific IgE, and bronchial provocation tests. Bronchial challenge with L. destructor provoked only a late phase asthmatic reaction without an immediate reaction despite the fact that immediate skin tests and specific IgE had been positive. Pretreatment with cromolyn sodium prevented any asthmatic reaction after bronchial challenge. An IgE immunologic mechanism could explain the clinical findings.
Subject(s)
Asthma/etiology , Mites/immunology , Occupational Exposure , Administration, Inhalation , Adult , Allergens/administration & dosage , Animals , Bronchial Provocation Tests , Edible Grain , Humans , Immunoglobulin E/analysis , Male , Precipitin Tests , Radioallergosorbent Test , Skin TestsABSTRACT
We report on a patient with asthma induced by occupational exposure to alpha-amylase derived from Aspergillus oryzae, which is a component of bread additives. A type I hypersensitivity to this enzyme was demonstrated by means of skin test, immunoassay for specific IgE, and immediate bronchial provocation test response to an alpha-amylase extract.
