Genetic insight into a polygenic trait using a novel genome-wide association approach in a wild amphibian population.

Body size variation is central in the evolution of life-history traits in amphibians, but the underlying genetic architecture of this complex trait is still largely unknown. Herein, we studied the genetic basis of body size and fecundity of the alternative morphotypes in a wild population of the Greek smooth newt (Lissotriton graecus). By combining a genome-wide association approach with linkage disequilibrium network analysis, we were able to identify clusters of highly correlated loci thus maximizing sequence data for downstream analysis. The putatively associated variants explained 12.8% to 44.5% of the total phenotypic variation in body size and were mapped to genes with functional roles in the regulation of gene expression and cell cycle processes. Our study is the first to provide insights into the genetic basis of complex traits in newts and provides a useful tool to identify loci potentially involved in fitness-related traits in small data sets from natural populations in non-model species.

Advances in Small Angle Neutron Scattering on Polysaccharide Materials.

Polysaccharide materials and biomaterials gain the focus of intense research owing to their great versatility in chemical structures and modification possibilities, as well as their biocompatibility, degradability, and sustainability features. This review focuses on the recent advances in the application of SANS on polysaccharide systems covering a broad range of materials such as nanoparticulate assemblies, hydrogels, nanocomposites, and plant-originating nanostructured systems. It motivates the use of SANS in its full potential by demonstrating the features of contrast variation and contrast matching methods and by reporting the methodologies for data analysis and interpretation. As these soft matter systems may be organized in multiple length scales depending on the interactions and chemical bonds between their components, SANS offers exceptional and unique opportunities for advanced characterization and optimization of new nanostructured polysaccharide materials.

Expression patterns of heat-shock genes during stopover and the trade-off between refueling and stress response in a passerine migrant.

Migrating birds are often exposed to variable environments and face a multitude of stress exposures along their long-distance flights. During stopover refueling, migratory birds must balance the need to accumulate energy reserves to continue their migration with the need to respond to environmental and physiological stressors. We examined the gene expression patterns of different Heat Shock Proteins (HSPs) in migrating birds during stopover at different body condition states (lean vs. fat), to provide some first insights on the role of HSPs in bird migration and explore the concept of a trade-off between refueling and stress response. Our results showed upregulation of HSP expression at release that could be associated with muscle growth and increased cholesterol and lipid synthesis needed for birds to fuel their upcoming migration. On the other hand, during capture, upregulation of HSP5 could be attributed to physiological recovery from the non-stop endurance flight when crossing the Sahara Desert-Mediterranean Sea ecological barrier. All birds significantly increased their fuel loads up to 48% of lean body mass and we provide evidence for muscle rebuilding during stopover as flight muscle mass increased by 10%, highlighting the fact that stopover sites can play a major role in the physiological recovery of migrants.

Postural balance ability and vertical jumping performance in female veteran volleyball athletes and non-athletes.

Lifetime participation in sports is associated with improved components of physical conditioning. The main purpose was to cross-sectionally investigate postural balance and vertical jumping performance in athletes with different histories of sports participation and secondarily to examine the restriction of vision on balance ability. A final aim was to investigate possible associations between balance and jumping performance. We hypothesized higher balance and jumping performance in active veteran volleyball athletes compared to retired athletes and non-athletes, suggesting a positive effect of continuous systematic training in active veteran athletes. We also hypothesized greater negative effect of vision removal on balance in the veteran compared to non-athletes due to athletes' stronger reliance on visual information. Eighty-one healthy middle-aged women (mean (standard deviation) 50 (5) years) were assigned to three experimental groups, a retired (n = 39, recreationally active former athletes), an active (n = 27, training 2days/week x 1.5 h/session) veteran volleyball athletes' and a control group (n = 15, sedentary participants). Participants completed an assessment of single-legged quiet stance trials with either left or right leg with eyes open while standing barefoot on a force plate and two-legged trials with both eyes open or closed. They also executed a protocol of countermovement jumps. Statistical analyses included univariate and full factorial ANOVAs with group and vision as fixed and repeated-measures factors and simple linear regression analysis. In the single-legged balance task, solely the mediolateral sway range was greater for the active (p < 0.001) and retired athletes (p < 0.001) compared to non-athletes, whereas in the two-legged stance, no differences among groups were found (p > 0.05). Restriction of vision deteriorated balance performance similarly in the three groups as a significant vision effect was found for path length (p < 0.001), anteroposterior (p < 0.001) and mediolateral sway (p < 0.05). The active and retired athletes had significantly (p < 0.001) greater height, mean and maximal power in countermovement jump compared to non-athletes. Results showed weak associations (average R2 = 9.5%) of balance with jumping performance only in the veteran volleyball athletes' group. Overall, the findings showed that retired volleyball athletes exhibited similar balance ability and vertical jumping performance as the active ones, suggesting a positive impact of prior experience in systematic training.

Adaptive Regulation of Stopover Refueling during Bird Migration: Insights from Whole Blood Transcriptomics.

Migration is one of the most energy-demanding tasks in avian life cycle. Many birds might not have sufficient fuel stores to cover long distances, so they must stop to rest and refuel at stopover sites, especially after the crossing of large ecological barriers. There, birds undergo several behavioral, morphological, and physiological trait adjustments to recover from and prepare for their journey; however, regulation of such processes at the molecular level remains largely unknown. In this study, we used transcriptomic information from the whole blood of migrating garden warblers (Sylvia borin) to identify key regulatory pathways related to adaptations for migration. Birds were temporarily caged during spring migration stopover and then sampled twice at different refueling states (lean vs. fat), reflecting different migratory stages (stopover arrival vs. departure) after the crossing of an extended ecological barrier. Our results show that top expressed genes during migration are involved in important pathways regarding adaptations to migration at high altitudes such as increase of aerobic capacity and angiogenesis. Gene expression profiles largely reflected the two experimental conditions with several enzymes involved in different aspects of metabolic activity being differentially expressed between states providing several candidate genes for future functional studies. Additionally, we identified several hub genes, upregulated in lean birds that could be involved in the extraordinary phenotypic flexibility in organ mass displayed by avian migrants. Finally, our approach provides novel evidence that regulation of water homeostasis may represent a significant adaptive mechanism, allowing birds to conserve water during long-distance flight, mainly through protein catabolism.

Effects of Drop Jump Training from Different Heights and Weight Training on Vertical Jump and Maximum Strength Performance in Female Volleyball Players.

ABSTRACT: Sotiropoulos, K, Smilios, I, Barzouka, K, Christou, M, Bogdanis, G, Douda, H, and Tokmakidis, SP. Effects of drop jump training from different heights and weight training on vertical jump, maximum strength and change of direction performance in female volleyball players. J Strength Cond Res 37(2): 423-431, 2023-This study compared the effects of drop jump (DJ) training from different drop heights and weight training on vertical jump and maximum strength performance. Fifty-five female volleyball players (age: 23.8 ± 4.3 years) were randomly and equally allocated to a control group (volleyball training, CG); a volleyball and weight training group (WG); and 3 volleyball, weight, and drop jump training groups. One group performed DJ training from the optimal drop height, i.e., the height that elicited the highest ratio of jump height to contact time (OG), a second group from a drop height 25% higher than the optimal (HG), and a third group from a drop height 25% lower than the optimal (LG). Drop jump and weight training were performed 1-2 times per week, for 8 weeks for a total of 13 sessions. After training, vertical jump performance improved by 3.6-17.4% ( p < 0.05; effect size [ES]: 1.03-1.23) in the OG and the HG compared with the LG, WG, and CG ( p < 0.05; ES: 0.03-0.58). Drop jump height from drop heights 20-70 cm increased by 10.0-20.2% ( p < 0.05; ES: 0.59-1.13) for the OG and the HG, while reactive strength index increased ( p < 0.05; ES: 0.74-1.40) by 19.6-33.9% only in the HG compared with the CG. Half-squat maximum strength was increased in all experimental groups by 17.4-19% compared with the CG ( p < 0.05) with no differences ( p > 0.05) observed among them. The use of the optimal height or a moderately higher drop height by 25% for DJ training, combined with weight training, seems to be the most beneficial option to improve vertical jump and reactive strength index in female volleyball players.

Current Advances of Polysaccharide-Based Nanogels and Microgels in Food and Biomedical Sciences.

Polysaccharides are natural polymers with hydrophilic, biocompatible and biodegradable characteristics and have many opportunities in the food and pharmaceutical sectors. This review focuses on the field of nano and microstructures whose internal structure is based on networked polysaccharide chains in 3D i.e., polysaccharide nanogels (NGs) and microgels (MGs). As it is observed the number of articles on NGs and MGs in peer reviewed scientific journals has been increasing over the last two decades. At the same time, the relative contribution of polysaccharides in this field is gaining place. This review focuses on the different applied methods for the fabrication of a variety of polysaccharide-based NGs and MGs and aims to highlight the recent advances on the subject and present their potentials and properties with regards to their integration in aspects of medicinal and food sciences. The presentation of the recent advances in the application of polysaccharide NGs and MGs is divided in materials with potential as emulsion stabilizers and materials with potential as carriers of bioactives. For applications in the medical sector the division is based on the fabrication processes and includes self-assembled, electrostatically complexed/ionically crosslinked and chemically crosslinked NGs and MGs. It is concluded that many advances are expected in the application of these polysaccharide-based materials and in particular as nutrient-loaded emulsion stabilizers, viscosity modifiers and co-assembled structures in combination with proteins.

Phylogeographic analyses point to long-term survival on the spot in micro-endemic Lycian salamanders.

Lycian salamanders (genus Lyciasalamandra) constitute an exceptional case of micro-endemism of an amphibian species on the Asian Minor mainland. These viviparous salamanders are confined to karstic limestone formations along the southern Anatolian coast and some islands. We here study the genetic differentiation within and among 118 populations of all seven Lyciasalamandra species across the entire genus' distribution. Based on circa 900 base pairs of fragments of the mitochondrial 16SrDNA and ATPase genes, we analysed the spatial haplotype distribution as well as the genetic structure and demographic history of populations. We used 253 geo-referenced populations and CHELSA climate data to infer species distribution models which we projected on climatic conditions of the Last Glacial Maximum (LGM). Within all but one species, distinct phyloclades were identified, which only in parts matched current taxonomy. Most haplotypes (78%) were private to single populations. Sometimes population genetic parameters showed contradicting results, although in several cases they indicated recent population expansion of phyloclades. Climatic suitability of localities currently inhabited by salamanders was significantly lower during the LGM compared to recent climate. All data indicated a strong degree of isolation among Lyciasalamandra populations, even within phyloclades. Given the sometimes high degree of haplotype differentiation between adjacent populations, they must have survived periods of deteriorated climates during the Quaternary on the spot. However, the alternative explanation of male biased dispersal combined with a pronounced female philopatry can only be excluded if independent nuclear data confirm this result.

Anthropometric and Motor Performance Variables Are Decisive Factors for The Selection of Junior National Female Volleyball Players.

This study examined whether anthropometric and fitness tests might successfully predict selection of young female volleyball players for a junior national team. Sixty four female players (age: 14.4 ± 0.5 y, body height: 1.76 ± 0.05 m, body mass: 63.9 ± 6.4 kg) underwent a selection procedure for the junior national team. Anthropometric data and speed and power test results were obtained and players were graded for their performance in a volleyball tournament. Selected players differed from the non-qualified in body height (3.4%; p = 0.001), standing reach height (2.6%; p = 0.001), the sum of skinfolds (15.4%; p = 0.035), body mass index (BMI; 7.1%; p = 0.005) and spike jump and reach (SJR) (2.5%; p = 0.001). Selected players were classified in the 99.2 ± 1.6 percentile in body height and in the 51.4 ± 20.6 percentile in the BMI, which were significantly different from those of the non-qualified players (95.4 ± 7.0 and 66.7 ± 18.6, p = 0.02 and p = 0.004, respectively). Stepwise discriminant analysis yielded a discriminant function (p < 0.001, η2 = 0.78) that was highly loaded by height, SJR and the BMI (r = 0.79, r = 0.74 and r = -0.53, respectively). Cross validation results showed that selection was correctly predicted in 15 out of the 20 selected players (predictive accuracy: 75.0%) and in 35 out of the 44 non-qualified players (predictive accuracy: 79.5%). In conclusion, body height, the BMI and SJR height successfully discriminated between selected and non-qualified elite young female junior national team volleyball players. The equal vertical jump, sprint and agility of selected and non-qualified players, highlight the importance of body height and the BMI for selection of elite junior female volleyball players.

Upper and Lower Body Power Are Strong Predictors for Selection of Male Junior National Volleyball Team Players.

Tsoukos, A, Drikos, S, Brown, LE, Sotiropoulos, K, Veligekas, P, and Bogdanis, GC. Upper and lower body power are strong predictors for selection of male junior National volleyball team players. J Strength Cond Res 33(10): 2760-2767, 2019-The purpose of this study was to determine whether a battery of anthropometric and lower and upper body strength and speed and power tests predicted selection of young volleyball players for a Junior National Team by expert coaches. Fifty-two male junior volleyball players (14.8 ± 0.5 years, height: 1.84 ± 0.05 m, body mass: 72.5 ± 7.1 kg) took part in a training camp and underwent a selection procedure by expert coaches' of the junior national team. Anthropometric data and fitness tests results were obtained and players were graded on a scale from 0 to 100 on the basis of their performance in a volleyball tournament. Selected players were superior in the majority of measured variables (p ≤ 0.017) and had higher grading scores compared with nonselected players (85.3 ± 4.1 vs. 70.5 ± 5.6, respectively, p < 0.01). The combination of spike jump and reach (SJR) test and 3-kg medicine ball throw (MB3) velocity explained 63.5% of the variance in expert coaches' grading (p < 0.001). A multivariate discriminant analysis yielded a significant discriminant function (Wilk's lambda = 0.55, χ = 29.324, p < 0.001, η = 0.82). Spike jump and reach and MB3 were the only variables that contributed to the discriminant function (standardized function coefficients: SJR = 0.68, MB3 = 0.67). Cross-validation results showed that selection was correctly predicted in 14 of the 16 selected players (predictive accuracy: 87.5%) and in 32 of the 36 nonselected players (predictive accuracy: 88.9%). The SJR and MB3 fitness tests can predict a large portion of the variance of expert coaches' grading and successfully discriminate elite young male volleyball players for selection vs. nonselection for a junior national team. This result is very important as performance testing during a selection process may be reduced to only 2 measurements.

Using genetic markers to unravel the origin of birds converging towards pre-migratory sites.

Identifying patterns of individual movements in spatial and temporal scales can provide valuable insight into the structure of populations and the dynamics of communities and ecosystems. Especially for migrating birds, that can face a variety of unfavorable conditions along their journey, resolving movements of individuals across their annual cycle is necessary in order to design better targeted conservation strategies. Here, we studied the movements of a small migratory falcon, the Lesser Kestrel (Falco naumanni), by genetically assigning feathers from individuals of unknown origin that concentrate in large roosts during the pre-migratory period. Our findings suggest that birds from multiple breeding populations in the Central and Eastern Mediterranean region move towards two pre-migratory sites in the Balkans, some of them detouring greatly from their expected flyways, travelling more than 500 km to reach these sites and prepare for the post-nuptial migration. By identifying the origin of individuals using the pre-migratory sites, not only we provide a better understanding of the possible impact of local threats at these sites on multiple breeding populations but also inform the design of effective conservation actions for the species.

Big Data Clustering via Community Detection and Hyperbolic Network Embedding in IoT Applications.

In this paper, we present a novel data clustering framework for big sensory data produced by IoT applications. Based on a network representation of the relations among multi-dimensional data, data clustering is mapped to node clustering over the produced data graphs. To address the potential very large scale of such datasets/graphs that test the limits of state-of-the-art approaches, we map the problem of data clustering to a community detection one over the corresponding data graphs. Specifically, we propose a novel computational approach for enhancing the traditional Girvan-Newman (GN) community detection algorithm via hyperbolic network embedding. The data dependency graph is embedded in the hyperbolic space via Rigel embedding, allowing more efficient computation of edge-betweenness centrality needed in the GN algorithm. This allows for more efficient clustering of the nodes of the data graph in terms of modularity, without sacrificing considerable accuracy. In order to study the operation of our approach with respect to enhancing GN community detection, we employ various representative types of artificial complex networks, such as scale-free, small-world and random geometric topologies, and frequently-employed benchmark datasets for demonstrating its efficacy in terms of data clustering via community detection. Furthermore, we provide a proof-of-concept evaluation by applying the proposed framework over multi-dimensional datasets obtained from an operational smart-city/building IoT infrastructure provided by the Federated Interoperable Semantic IoT/cloud Testbeds and Applications (FIESTA-IoT) testbed federation. It is shown that the proposed framework can be indeed used for community detection/data clustering and exploited in various other IoT applications, such as performing more energy-efficient smart-city/building sensing.

Modification of xanthan solution properties by the cationic surfactant DTMAB.

The interactions between xanthan gum (XG) and dodecyltrimethylammonium bromide (DTMAB) are studied by static and dynamic light scattering, microrheology and viscometry within the overlapping regime of aqueous xanthan solutions. In the absence of salt mixing with surfactant transforms the complex fluid of interconnected xanthan chains into diffusing aggregates because of the reduction of the polysaccharide's effective charge and the introduction of hydrophobic units. In the presence of salt the effect of complexation is weaker. Mixing the two components while XG is in its denatured state results to a network with reduced interchain connections. Linear (microrheological) and nonlinear (steady shear) viscoelastic experiments show that there is a significant compromise of the viscoelastic moduli which is remarkably more intense when mixing DTMAB with XG in its denatured state. These findings prove that xanthan's viscoelastic and morphological properties may be tuned by addition of surfactants and that the conformational state of XG during complexation is a defining parameter.

Hidden diversity in the Podarcis tauricus (Sauria, Lacertidae) species subgroup in the light of multilocus phylogeny and species delimitation.

The monophyletic species subgroup of Podarcis tauricus is distributed in the western and southern parts of the Balkans, and includes four species with unresolved and unstudied inter- and intra-specific phylogenetic relationships. Using sequence data from two mitochondrial and three nuclear genes and applying several phylogenetic methods and species delimitation approaches to an extensive dataset, we have reconstructed the phylogeny of the Podarcis wall lizards in the Balkans, and re-investigated the taxonomic status of the P. tauricus species subgroup. Multilocus analyses revealed that the aforementioned subgroup consists of five major clades, with P. melisellensis as its most basal taxon. Monophyly of P. tauricus sensu stricto is not supported, with one of the subspecies (P. t. ionicus) displaying great genetic diversity (hidden diversity or cryptic species). It comprises five, geographically distinct, subclades with genetic distances on the species level. Species delimitation approaches revealed nine species within the P. tauricus species subgroup (P. melisellensis, P. gaigeae, P. milensis, and six in the P. tauricus complex), underlining the necessity of taxonomic re-evaluation. We thus synonymize some previously recognized subspecies in this subgroup, elevate P. t. tauricus and P. g. gaigeae to the species level and suggest a distinct Albanian-Greek clade, provisionally named as the P. ionicus species complex. The latter clade comprises five unconfirmed candidate species that call for comprehensive studies in the future.

Contrast Loading Increases Upper Body Power Output in Junior Volleyball Athletes.

PURPOSE: This study examined the acute effects of contrast loading on mechanical power output during bench-press throws in junior volleyball players. METHOD: Eleven males (age: 16.5 ± 0.5 years) performed a contrast loading and a control protocol. The contrast protocol included the execution of 3 bench-throws with a 30% load of 1RM, after 3 min a conditioning set of 5 bench-throws with a 60% load of 1RM and after 3 and 5 min two more sets of 3 bench-throws with a 30% load of 1RM. The control protocol included the execution of 3 sets of 3 bench-throws with a 30% load of 1RM at the same time points as in the contrast protocol without the execution of the conditioning set. RESULTS: Mechanical power with a 30% load was higher (p < .05) 3 and 5 min following the conditioning set at the contrast protocol compared with the control protocol (8.7 ± 7.5 and 10.4 ± 3.4%, respectively). High correlations (p < .05) were obtained between participant's relative maximal strength (r = .87) and power (r = .82) and the increases in power output. CONCLUSION: Contrast loading increases upper body power output produced with a light load by junior athletes. The potential for increased upper body performance is more evident in stronger or more powerful individuals.

Red cell distribution width and mortality in acute heart failure patients with preserved and reduced ejection fraction.

BACKGROUND: Elevated red blood cell distribution width (RDW) is a valid predictor of outcome in acute heart failure (AHF). It is unknown whether elevated RDW remains predictive in AHF patients with either preserved left ventricular ejection fraction (LVEF) ≥50% or reduced LVEF (<50%). METHODS AND RESULTS: Prospective local registry including 402 consecutive hospitalized AHF patients without acute coronary syndrome or need of intensive care. The primary outcome was all-cause mortality (ACM) at 1 year after admission. Demographic and clinical data derive from admission, echocardiographic examinations (n = 269; 67%) from hospitalization. The Cox proportional hazard model including all patients (P < 0.001) was adjusted for age, gender, and RDW quartiles. Independent predictors of 1-year ACM were cardiogenic shock (HR 2.86; CI: 1.3-6.4), male sex (HR 1.9; CI: 1.2-2.9), high RDW quartile (HR 1.66; CI: 1.02-2.8), chronic HF (HR 1.61; CI: 1.05-2.5), valvular heart disease (HR 1.61; CI: 1.09-2.4), increased diastolic blood pressure (HR 1.02 per mmHg; CI: 1.01-1.03), increasing age (HR 1.04 by year; CI: 1.02-1.07), platelet count (HR 1.002 per G/l; CI: 1.0-1.004), systolic blood pressure (HR 0.99 per mmHg; CI: 0.98-0.99), and weight (HR 0.98 per kg; CI: 0.97-0.99). A total of 114 patients (28.4%) died within the first year; ACM of all patients increased with quartiles of rising RDW (χ2 18; P < 0.001). ACM was not different between RDW quartiles of patients with reduced LVEF (n = 153; χ2 6.6; P = 0.084). In AHF with LVEF ≥50% the probability of ACM increased with rising RDW (n = 116; χ2 9.9; P = 0.0195). CONCLUSIONS: High RDW is associated with increased ACM in AHF patients with preserved but not with reduced LVEF in this study population.

Scattering investigation of multiscale organization in aqueous solutions of native xanthan.

The hierarchical morphology of xanthan solutions is analyzed by light and neutron scattering in a broad range of concentrations in order to connect their morphology to their well-documented dynamic properties. Static light scattering inside the semidilute regime is dominated by the form factor of individual xanthan chains while at higher concentrations chain interconnections appear to modify the low wave vector scattering. Dynamic light scattering reveals the self-similar nature of the solutions caused by interchain associations as intensity autocorrelation functions present power-law behaviour. Small angle neutron scattering is dominated by the fractal scattering from the formed network at intermediate length scales. At small length scales the rigid structure of xanthan is revealed and the molecular weight per unit length is extracted. No detectable morphological alterations for shear rates up to 1000rad/s are observed revealing that the shear thinning behaviour of xanthan is related to the disruption of chain-chain associations.

Disseminated cutaneous infection with Mycobacterium chelonae in a renal transplant recipient.

Mycobacterium chelonae belongs to a rapidly growing group of nontuberculous mycobacteria (NTM). These organisms are environmental saprophytes that can cause infection in humans. Nontuberculous mycobacteria infections have been described in immunosuppressed patients (eg, in the setting of AIDS or immunotherapy following solid organ transplantation) as well as in immunocompetent patients with certain predisposing factors (eg, recent history of a traumatic wound, recent drug injections, impaired cell-mediated immunity). Due to the increasing prevalence of immune deficiency disorders as well as the rising number of cosmetic procedures performed on healthy individuals, NTM may become a frequent cause of serious morbidity, causing chronic infections of the skin, soft tissue, and lungs. We report a case of M chelonae infection in a 61-year-old woman who was receiving immunosuppressive therapy following renal transplantation 6 years prior to presentation. It is important for clinicians to consider NTM in the differential diagnosis for patients who present with chronic skin or soft tissue infections.

Contrast loading: power output and rest interval effects on neuromuscular performance.

PURPOSE: This study examined the effect of rest interval after the execution of a jump-squat set with varied external mechanical-power outputs on repeated-jump (RJ) height, mechanical power, and electromyographic (EMG) activity. METHODS: Twelve male volleyball players executed 6 RJs before and 1, 3, 5, 7, and 10 min after the execution of 6 repetitions of jump squats with a load: maximized mechanical-power output (Pmax), 70% of Pmax, 130% of Pmax, and control, without extra load. RESULTS: RJ height did not change (P = .44) after the jump squats, mechanical power was higher (P = .02) 5 min after the 130%Pmax protocol, and EMG activity was higher (P = .001) after all exercise protocols compared with control. Irrespective of the time point, however, when the highest RJ set for each individual was analyzed, height, mechanical power, and EMG activity were higher (P = .001-.04) after all loading protocols compared with control, with no differences observed (P = .53-.72) among loads. CONCLUSIONS: Rest duration for a contrast-training session should be individually determined regardless of the load and mechanical-power output used to activate the neuromuscular system. The load that maximizes external mechanical-power output compared with a heavier or a lighter load, using the jump-squat exercise, is not more effective for increasing jumping performance afterward.