Potential Challenges of the Extraction of Carotenoids and Fatty Acids from Pequi (Caryocar brasiliense) Oil.

Pequi is a natural source of bioactive compounds with wide versatility for fresh or processed fruit consumption, but it is still little explored economically. Functional foods are the subject of diverse scientific research since, in addition to being nourishing, they contain bioactive compounds capable of promoting several benefits to the human body. Pequi is a fruit species native to the Brazilian Cerrado, which is rich in oil and has components with a high nutritional value, such as unsaturated fatty acids (omega-3, omega-6, EPA, and DHA), antioxidants (carotenoids and phenolic compounds), and vitamins. Therefore, the present narrative review aims to compile and critically evaluate the methods used to extract oil from the pulp and almonds of pequi and describes the carotenoid separation from the oil because carotenoids are natural pigments of great interest in the pharmaceutical and food industries. It is emphasized that the main challenges linked to bioactive compound extraction are their susceptibility to degradation in the processing and storage stages of pequi and its derived products.

Implementation of the nursing process in an intermediate care unit / Implementación del proceso de enfermería en una unidad de cuidados intermedios / Implantação do processo de enfermagem em unidade de cuidados intermediários

Objective: to describe the implementation of the Nursing Process in an intermediate care unit. Method: action research conducted in a public teaching hospital from December 2019 to March 2020 with four training on Nursing Process and Nursing Theories; presentation of nursing process instruments, implementation and follow-up. Changes suggested by the nurses occurred through participant observation and field diary in in loco visits. Results: difficulties in initial filling of instruments were reported, as well as practical inexperience and lack of time and work overload. The benefits mentioned were related to the clear and orderly formatting of the instruments; ease in the application of steps and safety in care practice. Final considerations: the implementation of the Nursing Process in the sector occurred after the identification of the previous knowledge of the nursing team, followed by the survey of the profile of patients, creation of instruments, execution of permanent education of professionals, application and monitoring in the use of the instruments.

Objetivo: describir la implementación del Proceso de Enfermería en una unidad de cuidados intermedios. Método: investigación-acción realizada en un hospital docente público de diciembre de 2019 a marzo de 2020 con cuatro capacitaciones sobre Procesos de Enfermería y Teorías de Enfermería; presentación de instrumentos del proceso de enfermería, implementación y seguimiento. Los cambios sugeridos por las enfermeras ocurrieron a través de la observación participante y el diario de campo en las visitas in loco. Resultados: se reportaron dificultades en el llenado inicial de los instrumentos; inexperiencia práctica; falta de tiempo y sobrecarga de trabajo. Los beneficios mencionados estaban relacionados con el formato claro y ordenado de los instrumentos; facilidad en la aplicación de pasos y seguridad en la práctica asistencial. Consideraciones finales: la implementación del Proceso de Enfermería en el sector ocurrió después de la identificación de los conocimientos previos del equipo de enfermería, seguido de la encuesta del perfil de los pacientes, creación de instrumentos, realización de educación permanente de los profesionales, aplicación y seguimiento en el uso de los instrumentos.

Objetivo: descrever a implantação do Processo de Enfermagem em uma unidade de cuidados intermediários. Método: pesquisa-ação realizada em hospital público de ensino de dezembro de 2019 a março de 2020 contando com quatro capacitações sobre Processo de Enfermagem e Teorias de Enfermagem; apresentação dos instrumentos do Processo de Enfermagem, implantação e acompanhamento. Alterações sugeridas pelos enfermeiros ocorreram mediante observação participante e diário de campo nas visitas in loco. Resultados: relatadas dificuldades no preenchimento inicial dos instrumentos; inexperiência prática; falta de tempo e sobrecarga de trabalho. Os benefícios mencionados foram relacionados à formatação clara e ordenada dos instrumentos; facilidade na aplicação das etapas e segurança na prática assistencial. Considerações finais: a implantação do Processo de Enfermagem no setor ocorreu após a identificação do conhecimento prévio da equipe de Enfermagem, seguida do levantamento do perfil de pacientes, criação dos instrumentos, realização de educação permanente dos profissionais, aplicação e acompanhamento no uso dos instrumentos.

Pulp mill sludge-derived carbon activated with an alternative source of chemicals and its application in wastewater treatment - An approach for byproducts valorization.

The purpose of this study was to investigate pulp mill by-products valorization through activated carbon (AC) production and its application in wastewater treatment. Bio-sludge is a known promising precursor for AC production. The mill's chemical recovery stage generates an effluent (electrostatic precipitator ash leachate - EPAL) with high levels of potassium and sodium, which motivate studies regarding its viability as a chemical source for carbon activation. Bio-sludge and EPAL are problematic by-products and this research line would allow their return to the productive chain, as an adsorbent for the wastewater treatment. Two carbonization heating rates (3.5, 15 °C/min), three activation agents (NaOH, KOH, EPAL) and two activator:bio-sludge ratios (1:1, 2:1) were used for AC production. The best ACs in terms of surface area were those produced at 3.5 °C/min, with KOH or EPAL in 2:1 proportion. ACs produced under these conditions and commercial activated carbon (CAC) were used in adsorption tests with industrial wastewater. For color removal, KOH-activated carbon presented the greatest efficiency (80.45 %), followed by CAC (76.74 %) and EPAL-activated carbon (70.13 %). For COD removal, EPAL-activated carbon presented greater efficiency (53.49 %), followed by CAC (40.84 %) and KOH-activated carbon (36.86 %). Freundlich's model best described the experimental adsorption data. The KOH results were expected to be satisfactory, since KOH is proven to be effective for carbon activation. The EPAL-activated carbon results were remarkable, especially for COD removal, showing that EPAL can be used as an activator and that the by-products have potential for valorization according to the circular economy principles.

Simulation of ethanol recovery and economic analysis of pectin production on an industrial scale.

Taking into account that the industrial processing of passion fruit generates significant amounts of waste (only the peels represent 51% of the total mass of the fruit), in the present study an economic analysis was conducted to evaluate industrial line viability for pectin extraction from passion fruit peels. Knowing that absolute ethanol (99.50% purity), used in the precipitation and washing steps, has a higher cost, a simulation of extractive distillation was performed using solvents ethylene glycol and glycerol, in the software Aspen Plus v.11, being possible to recover 99.63% of ethanol for both solvents. The results of the economic evaluation showed that the process using ethylene glycol has an advantage, mainly due to its higher profitability (1.13 times higher), lower production cost (94.86% of the price using glycerol), and a lower breakeven point (around 3% smaller). The financial indicators showed profitability and attractiveness for the implementation of this processing line.

Understanding the Effect of Ionic Liquids as Adjuvants in the Partition of Biomolecules in Aqueous Two-Phase Systems Formed by Polymers and Weak Salting-Out Agents.

Ionic liquids (ILs) as adjuvants in polymer-salt aqueous two-phase systems (ATPS) have been used to improve the extraction of biomolecules. However, the impact of ILs as adjuvants on the partition of biomolecules is still poorly understood. Previous works mostly focused on ATPS based on strong salting-out agents, which may mask the IL effect. In this work, ATPS formed by polyethylene glycol (PEG 400) and a weak salting-out salt ((NH4)2SO4) with a wide number of ILs as adjuvants (chloride-based combined with cholinium, imidazolium, pyrrolidinium, piperidinium, tetralkylammonium and tetralkylphosphonium cations) were investigated. The respective phase diagrams were determined, and the systems extraction performance for a wide range of biomolecules (phenolic compounds, alkaloids and amino acids) was investigated. The results obtained show that ILs as adjuvants in polymer-salt ATPS modulate the partition of biomolecules. In particular, more hydrophobic ILs significantly enhance the partition of more hydrophobic biomolecules to the PEG-rich phase (where the IL is enriched). Furthermore, the intensity of the IL effect is more pronounced when using weak salting-out agents. A linear correlation between the biomolecules and the ILs partition coefficients, and with the biomolecules octanol-water partition coefficients, was found. In most ATPS formed by polymers and salts using ILs as adjuvants, the biomolecules partition is driven by the ILs partition and by the difference in hydrophobicity between the coexisting phases.

Potential of Aqueous Two-Phase Systems for the Separation of Levodopa from Similar Biomolecules.

BACKGROUND: Levodopa is a precursor of several neurotransmitters, such as dopamine, and is used in the treatment of the Parkinson's disease. In this work, an alternative strategy was studied to separate levodopa from similar biomolecules using aqueous two-phase systems (ATPS). RESULTS: Ternary ATPS composed of polyethylene glycol (PEG) 400 or ionic liquids (ILs), citrate buffer (K3C6H5O7/C6H8O7) at pH 7.0 and water, and quaternary ATPS composed of PEG 400, K3C6H5O7/C6H8O7 at pH 7.0, water and the same ILs at 5 wt%, were studied. The respective liquid-liquid phase diagrams were determined at 298 K to appraise the mixture compositions required to form two-phase systems, followed by studies of the partition of levodopa and structurally similar biomolecules (dopamine, L-phenylalanine, and L-tyrosine). Their partition coefficients and extraction efficiencies have been determined, and the selectivity of the ATPS to separate levodopa from the remaining biomolecules evaluated. CONCLUSION: The results obtained indicated that PEG-based ATPS were the most effective to separate levodopa from L-phenylalanine while the separation from the other biomolecules was better using IL-based ATPS, in particular those based on [P4444]Cl and [N4444]Cl, with extraction efficiencies of levodopa to the salt-rich phase ranging between 62.7 and 74.0%, and of the remaining biomolecules to polymer/IL-rich phase up to 91.5%.

Particularidades de idosos hipertensos à adesão ao tratamento medicamentoso / Particularities of hypertensive elderly people to medicinal treatment adherence

Objetivo: verificar em idosos hipertensos as particularidades que envolvem a adesão ao tratamento medicamentoso. Método: estudo qualitativo, descritivo e exploratório, com 20 idosos hipertensos, participantes de um Projeto de Extensão desenvolvido por uma Instituição de Ensino Particular, a partir de entrevista semiestruturada analisada pela Técnica de Análise de Conteúdo. Resultados: existe conhecimento dos idosos acerca da hipertensão e suas repercussões no organismo, como também há adesão ao tratamento perante o autocuidado e cuidados familiares. Conclusão: a compreensão em relação ao processo saúde e doença permite que intervenções terapêuticas sejam realizadas de forma menos biologicista, priorizando a interação entre os profissionais da saúde, idosos e familiares que convivem com a hipertensão dentro de um sistema de saúde funcional.(AU)

The Process of Separating Bovine Serum Albumin Using Hydroxyapatite and Active Babassu Coal (Orbignya martiana).

Bovine serum albumin is one of the major serum proteins; it plays an important role as a result of its functional and nutritional properties which have bioactive peptides. Adsorption method was used to separate protein, which involves hydroxyapatite, synthetic hydroxyapatite, and active babassu coal. Initially, characterization was carried out using the zeta potential of the adsorbents. Kinetic pseudo-first- and pseudo-second-order models were applied. For isotherms, equilibrium data studies were carried out using the Langmuir and Freundlich models, in addition to determining the efficiency of adsorptive process. The results of the zeta potential showed loads ranging from +6.9 to -42.8 mV. The kinetic data were better represented in the pseudo-second-order model with chemisorption characteristics. The adsorption capacity of the adsorbents decreased as pH increased, indicating that the electrostatic bonds and some functional groups of active babassu coal contributed to the reduction of adsorption, especially oxygen linked to carbon atoms. The value of pH 4.0 showed the best results of adsorption, being obtained as the maximum adsorption capacity (q m ) and yield (%) (where q m = 87.95 mg g(-1) and 74.2%; 68.26 mg g(-1) and 68.6%; and 36.18 mg g(-1), 37.4%) of hydroxyapatite, synthetic hydroxyapatite, and active babassu coal, respectively.

[Latinamerican guidelines of RIICER for diagnosis of tick-borne rickettsioses]. / Guías Latinoamericanas de la RIICER para el diagnóstico de las rickettsiosis transmitidas por garrapatas.

Tick-borne rickettsioses are worldwide infectious diseases that are considered emerging and re-emerging. Until recently the only tick-borne rickettsiosis present in Latin America was Rickettsia rickettsii infection, but to date, with the incorporation of new tools as PCR and sequencing and the quick cellular close tube cultures (Shell-vial), new species has been involved as human pathogens. In these guidelines, we offer an update of the microbiological assays for diagnosing rickettsioses. Besides we have included a section in which the most important hard ticks involved in human rickettsioses in Latinoamerica are detailed.

Guías Latinoamericanas de la RIICER para el diagnóstico de las rickettsiosis transmitidas por garrapatas / Latinamerican guidelines of RIICER for diagnosis of tick-borne rickettsioses

Tick-borne rickettsioses are worldwide infectious diseases that are considered emerging and re-emerging. Until recently the only tick-borne rickettsiosis present in Latin America was Rickettsia rickettsii infection, but to date, with the incorporation of new tools as PCR and sequencing and the quick cellular close tube cultures (Shell-vial), new species has been involved as human pathogens. In these guidelines, we offer an update of the microbiological assays for diagnosing rickettsioses. Besides we have included a section in which the most important hard ticks involved in human rickettsioses in Latinoamerica are detailed.

Las rickettsiosis transmitidas por garrapatas son afecciones de distribución mundial, que por diferentes motivos se pueden considerar emergentes y reemergentes. Hasta hace escasos años la única rickettsiosis transmitida por garrapatas en Latinoamérica era la infección por Rickettsia rickettsii, pero en la actualidad y fundamentalmente, gracias a la incorporación de nuevas herramientas para el diagnóstico microbiológico como la reacción en cadena de la polimerasa y secuenciación o el cultivo celular rápido en tubo cerrado, se han descrito e involucrado otras especies de Rickettsia en la producción de patología humana. En estas guías se detallan y describen las diferentes técnicas utilizadas para el diagnóstico microbiológico de las rickettsiosis. Además, se incluye una sección en la que se detallan las especies más importantes de garrapatas duras relacionadas con las rickettsiosis en Latinoamérica, con claves para su clasificación taxonómica.

Thermodynamic studies of partitioning behavior of lysozyme and conalbumin in aqueous two-phase systems.

The objective of this study was to determine the thermodynamic parameters (Delta(tr)G, Delta(tr)H and Delta(tr)S) associated with lysozyme and conalbumin partitioning in aqueous two-phases systems (ATPS). Influence of salt type and polyethylene glycol (PEG) concentrations on the partition coefficient of lysozyme and conalbumin from egg white was studied. The evaluated ATPS were composed of PEG 1500 and inorganic salts (sodium citrate and sodium sulfate) at a temperature of 25 degrees C and pH 7.0, with PEG 1500 g mol(-1) concentrations of 14%, 16% and 18% (mass basis). Partitioning of lysozyme in PEG-citrate ATPS was enthalpically driven, however the PEG-sulfate ATPS was entropically driven. The tested systems can be employed for the separation of these two proteins in egg white, due to the fact that lysozyme migrates toward the polymeric phase and conalbumin to the saline phase in both ATPS. A high recovery of conalbumin in the saline phase of the PEG-sulfate ATPS was determined to be enthalpically driven.

Flora arbustivo-arbórea do Parque Estadual do Jaraguá, São Paulo - SP / Floristic composition of tree and shrub species of the Jaraguá State Park, São Paulo - SP, Brazil

O objetivo deste trabalho foi caracterizar a flora arbustivo-arbórea do Parque Estadual do Jaraguá (PEJ), a fim de contribuir para o conhecimento de sua diversidade. Foram feitas coletas mensais de material reprodutivo dos indivíduos arbustivos e arbóreos ao longo de três trilhas, durante um período de 12 meses. O levantamento resultou em uma lista florística contendo 262 espécies nativas, de 153 gêneros e 55 famílias. As famílias mais representativas foram Fabaceae, com 30 espécies, Myrtaceae (26), Asteraceae (24), Melastomataceae (20), Lauraceae (16) e Rubiaceae (15). Assim como relatado para outros remanescentes da região metropolitana de São Paulo e entorno, a vegetação do PEJ está situada em uma zona de transição, onde predomina a floresta ombrófila densa, com a presença de algumas espécies de floresta estacional semidecidual. Destaca-se ainda a existência de uma outra fitofisionomia em uma região de afloramentos de rocha e solo raso, onde ocorrem espécies de cerrado, e que merece estudos específicos. Nessa área, a vegetação é predominantemente herbácea, com árvores e arbustos esparsos, em geral de pequeno porte e com tronco suberoso. Especificamente nessa formação, foram registradas 55 espécies pertencentes a 41 gêneros e 18 famílias, das quais 22 não ocorreram nos outros locais amostrados. A família com maior riqueza foi Asteraceae (15 espécies), seguida por Myrtaceae (7), Fabaceae (5) e Melastomataceae (4). Em todo o levantamento, foram contabilizadas 20 espécies exóticas. Apesar de grande parte da vegetação do Parque ser secundária e estar exposta a fortes pressões antrópicas, foram encontradas 14 espécies presentes em alguma das categorias existentes nas listas oficiais de espécies ameaçadas, reforçando a importância do PEJ para a conservação da biodiversidade.

The objective of this study was to characterize the floristic composition of trees and shrubs of the Jaraguá State Park (PEJ), contributing to the knowledge of its biodiversity. Reproductive botanic material was collected along three trails during a 12-month period. The field survey resulted in a flora of 262 native species, 153 genera and 55 families. Fabaceae was the richest family (30 species), followed by Myrtaceae (26), Asteraceae (24), Melastomataceae (20), Lauraceae (16) and Rubiaceae (15). As mentioned for other forest remnants in São Paulo metropolitan region and surroundings, the PEJ vegetation is in a transition zone, where the main formation is the ombrophilous dense forest, but where species of seasonal semi-deciduous forest also occur. Besides that, there is a completely different phytophysiognomy occurring in a region with rocky outcrops and low depth soils, in which cerrado species were found, and that deserves more specific studies. In this site, the vegetation of grasses is the most abundant, and there are also shrubs and trees sparsely distributed, many of them with low height and suberous trunk. Most specifically in this area there were 55 species of 41 genera and 18 families, from which 22 did not occur in the other sites visited. Asteraceae was the family with the higher number of species (15), followed by Myrtaceae (7), Fabaceae (5) and Melastomataceae (4). Twenty exotic species were found along the trails. Despite the fact that PEJ has a big area of secondary vegetation and that it is exposed to strong anthropogenic pressures, there were 14 species belonging to some threat category defined at the official red lists, reinforcing the importance of the Park for biodiversity conservation.

PCR-based survey of Anaplasma phagocytophilum in Portuguese ticks (Acari: Ixodidae).

A total of two-thousand and six ticks, collected from 2002 to 2006 in areas belonging to seven districts of Mainland Portugal and also in Madeira Island, were examined by polymerase chain reaction (PCR) for the presence of Anaplasma phagocytophilum. Active infections were detected exclusively in Ixodes species, including six questing I. ricinus nymphs from Madeira Island, one questing I. ventalloi nymph from Setúbal District, and two I. ventalloi adults found parasitizing domestic cats in both Setúbal and Santarém District. These findings confirm prior observations and suggest the persistence of A. phagocytophilum on Madeira Island. Moreover, it adds I. ventalloi and domestic cats to the list of potential elements of the agent's enzootic cycles in Portugal. Molecular analysis of PCR amplicons suggests the existence of two A. phagocytophilum genotypes in Portugal, one of which is identical or very similar to North American strains implicated in human disease.

Detection of Ehrlichia canis by polymerase chain reaction in dogs from Portugal.

Antibodies against Ehrlichia canis, the cause of canine monocytic ehrlichiosis, have been reported previously in clinically ill and stray dogs from Portugal. In this study, the 16S rRNA gene of E. canis was detected by the polymerase chain reaction (PCR) in 12/55 (22%) of dogs with suspected tick-borne disease in the Algarve region in Portugal.

Host- and microbe-related risk factors for and pathophysiology of fatal Rickettsia conorii infection in Portuguese patients.

BACKGROUND: The pathophysiologic mechanisms that determine the severity of Mediterranean spotted fever (MSF) and the host-related and microbe-related risk factors for a fatal outcome are incompletely understood. METHODS: This prospective study used univariate and multivariate analyses to determine the risk factors for a fatal outcome for 140 patients with Rickettsia conorii infection admitted to 13 Portuguese hospitals during 1994-2006 with documented identification of the rickettsial strain causing their infection. RESULTS: A total of 71 patients (51%) were infected with the Malish strain of Rickettsia conorii, and 69 (49%) were infected with the Israeli spotted fever (ISF) strain. Patients were admitted to the intensive care unit (40 [29%]), hospitalized as routine inpatients (95[67%]), or managed as outpatients (5[4%]). Death occurred in 29 adults (21%). A fatal outcome was significantly more likely for patients infected with the ISF strain, and alcoholism was a risk factor. The pathophysiology of a fatal outcome involved significantly greater incidence of petechial rash, gastrointestinal symptoms, obtundation and/or confusion, dehydration, tachypnea, hepatomegaly, leukocytosis, coagulopathy, azotemia, hyperbilirubinemia, and elevated levels of hepatic enzymes and creatine kinase. Some, but not all, of these findings were observed more often in ISF strain-infected patients. CONCLUSIONS: Although fatalities and similar clinical manifestations occurred among both groups of patients, the ISF strain was more virulent than the Malish strain. Multivariate analysis revealed that acute renal failure and hyperbilirubinemia were most strongly associated with a fatal outcome.

[Rickettsial diseases in Brazil and Portugal: occurrence, distribution and diagnosis]. / Riquetsioses no Brasil e Portugal: ocorrência, distribuição e diagnóstico.

The present study is an update review on the occurrence and diagnosis of rickettsial diseases in Brazil and Portugal, aiming at promoting their epidemiological surveillance in both countries. A literature review was carried out and unpublished data of laboratories and surveillance systems were presented. The results described the occurrence of rickettsial diseases and infections in Brazil and Portugal, including other new and still poorly understood rickettsial infections. Current diagnostic methods were discussed. As in many other countries, rickettsial diseases and infections seem to be an emerging public health problem. Treated as a minor problem for many decades, the interest in these infections has increased in both countries but further studies are needed to establish their role as a public health problem.

Riquetsioses no Brasil e Portugal: ocorrência, distribuição e diagnóstico / Rickettsial diseases in Brazil and Portugal: occurrence, distribution and diagnosis

O presente artigo é uma atualização sobre a ocorrência e diagnóstico das riquetsioses existentes no Brasil e Portugal, com o objetivo de incentivar e incrementar a vigilância epidemiológica dessas doenças nos dois países. Realizou-se levantamento bibliográfico e foram apresentados dados não publicados de laboratórios e serviços de epidemiologia. Os resultados descreveram a ocorrência das riquetsioses no Brasil e Portugal, inclusive aquelas recém-descritas, advindas de riquétsias de potencial patogênico ainda incerto. Os métodos diagnósticos atualmente empregados foram discutidos. Como em outros países, as riquetsioses parecem assumir crescente importância em saúde pública. Relegadas a um plano secundário por muitas décadas, o interesse por essas infecções tem aumentado nos dois países, mas ainda carece de investigação para esclarecer seu real significado em saúde pública.

[Epidemiologic features of Mediterranean spotted fever in Portugal]. / Sobre a realidade da febre escaro-nodular em Portugal.

Mediterranean spotted fever (MSF) is a tick-borne rickettsial disease. It is endemic in Portugal and Ricardo Jorge recognized it in 1930. Rhipicephalus sanguineus is the main vector and reservoir of the disease. In Portugal the etiologic agents are two strains of Rickettsia conorii complex: R. conorii Malish and "Israeli tick typhus". MSF is clinically characterized by a vasculitis process with the classical clinical triad of fever, rash, and lesion at the site of tick bite. Although the majority MSF cases are considered benign, last years some districts presented more severe cases than expected. The reported incidence of MSF in Portugal for 1989 to 2000 was 9.8/10(5) inhabitants, the highest incidence compared to other mediterranean countries. Alentejo was the region who presents the highest incidence with 31/10(5) inhabitants. Nevertheless, Bragança was the district which appears with greater number of cases, 62/10(5) inhabitants. The highest annual incidence rate reported was among children, peaking in those 1-4 years of age with 60.2/10(5) inhabitants. Observation of mortality rates have shown that Beja district have the highest mortality rates for MSF in Portugal, but in 1997 in this district, mortality have unexpected values with 7.6 deaths per 100,000 inhabitants have occurred. Although is an obligatory notifiable disease, high number of underreported cases are detected.