ABSTRACT
The complete mitochondrial genome of the Ruby-Topaz Hummingbird, Chrysolampis mosquitus, was determined using 1/11 of an Illumina Hi-seq lane ran with a Nextera kit. We assembled the mitogenome in a two-step approach using both (i) de novo (SOAPdenovo-Trans) and (ii) reference-based (MITObim) genome assembly software. A circular molecule containing 17,767 bp was assembled. As expected for most vertebrates, the C. mosquitus mitogenome contained 13 protein-coding genes, 22 transfer RNA, 2 ribosomal RNA genes, and 1 non-coding control region. We assembled the whole mitogenome using 0.45% of the total amount of reads and produced a high-coverage mitochondrial genome (>1000×). We deposited the assembled mitogenome into GenBank (accession number KJ619585).
Subject(s)
Birds/genetics , Genome, Mitochondrial , Sequence Analysis, DNA/methods , Animals , Base Composition/genetics , Base Sequence , DNA, Mitochondrial/geneticsABSTRACT
The genome of the versicoloured emerald hummingbird (Amazilia versicolor) was partially sequenced in one-sixth of an Illumina HiSeq lane. The mitochondrial genome was assembled using MIRA and MITObim software, yielding a circular molecule of 16,861 bp in length and deposited in GenBank under the accession number KF624601. The mitogenome contained 13 protein-coding genes, 22 transfer tRNAs, 2 ribosomal RNAs and 1 non-coding control region. The molecule was assembled using 21,927 sequencing reads of 100 bp each, resulting in â¼130 × coverage of uniformly distributed reads along the genome. This is the forth mitochondrial genome described for this highly diverse family of birds and may benefit further phylogenetic, phylogeographic, population genetic and species delimitation studies of hummingbirds.
Subject(s)
Biodiversity , Birds/classification , Birds/genetics , Genome, Mitochondrial , Animals , Base Composition , Codon , Computational Biology/methods , Genes, Mitochondrial , Genome Size , Open Reading Frames , Sequence Analysis, DNA , Whole Genome SequencingABSTRACT
Cerca de 90 a 95% de todos os tipos de câncer estão associados ao estilo de vida, sendo que um terço se correlaciona à alimentação, incluindo bebidas alcoólicas. Neste estudo, procuramos identificar a percepção pública de brasileiros sobre álcool e câncer. Foi realizada uma pesquisa construída com o auxílio da ferramenta online SurveyMonkey® divulgada pelo Facebook®. A amostra contou com 231 participantes entre 13 e 45anos, de 25 diferentes estados brasileiros e de todas as classes sociais. A maioria dos participantes (83%) acredita que a propaganda de bebidas alcóolicas influencia os outros, mas não a si próprios (87%). Baseadonos resultados, sugerimos uma estratégia de comunicação em saúde...
Approximately 90-95% of all cancers are associated with lifestyle, and a third of them is correlated to food,including alcohol consumption. In this study, we try to identify public perception of Brazilian alcohol consumptionand cancer. We did a survey constructed with an online tool: the SurveyMonkey® and announcedby Facebook®. The sample included 231 participants between 13 and 45 years old, from 25 different Brazilianstates and from all social classes. Brazilian people (83%) believe that alcohol advertisements influenceothers, but not themselves (87%). Based on the results, we suggest a strategy for health communication...
Aproximadamente 90 a 95% de todos los cánceres están asociados con el estilo de vida, y un tercio serelaciona con los alimentos, incluidas las bebidas alcohólicas. Este estudio trata de identificar la percepciónpública de los brasileños acerca del alcohol y del cáncer. Se realizó una búsqueda construida con la ayuda dela herramienta online SurveyMonkey® lanzada por el Facebook®. La muestra incluyó 231 participantesentre 13 y 45 años, procedentes de 25 estados brasileños diferentes y de todas las clases sociales. La mayoríade los participantes (83%) cree que la publicidad de la bebida alcohólica afecta a los demás, pero no a símismos (87%). Con base en los resultados, sugerimos una estrategia de comunicación en salud...
