General low alertness in people with obstructive sleep apnea.

STUDY OBJECTIVES: In patients with obstructive sleep apnea (OSA), a previous study using a Go/No-Go task reported an average attention deficit. However, the temporal dynamics of such a deficit are unknown. Here, we investigated whether attention deficits in different subdomains increased as the test progressed. We also investigated the effect of target frequency and speed of stimulus presentation on performance. METHODS: Twenty-seven untreated people with OSA and 27 age- and sex-matched controls underwent a 15-minute Go/No-Go task, divided into 6 blocks. Each block was subdivided into 3 different interstimulus intervals (1, 2, and 4 seconds). Three blocks had a low and three had a high target probability (20% and 80%, respectively). Reaction time (alertness), variability of reaction time (sustained attention), commission errors (response inhibition), and omission errors (focused attention) were measured. RESULTS: Alertness was lower in the group with OSA compared with controls, as evidenced by a significantly higher average reaction time. This effect was seen from the start of the task and continued until the end but did not increase in test progression. The temporal pattern of intrinsic alertness deficits in patients with OSA was found to be independent of target frequency or interstimulus interval. CONCLUSIONS: The primary attention problem in OSA is on the alertness subdomain irrespective of the number of required responses or speed of stimulus presentation. The present results support the notion that OSA is distinct from other neurological and psychiatric conditions, such as depression or chronic pain. The results also suggest significant concerns regarding daily life activities (eg, driving). CITATION: de Souza Bezerra ML, van Duinkerken E, Simões E, Schmidt SL. General low alertness in people with obstructive sleep apnea. J Clin Sleep Med. 2024;20(5):689-698.

Performance of collagen-based matrices from Nile tilapia skin: A pilot proteomic study in a murine model of wound healing.

Full-thickness cutaneous trauma, due to the lack of dermis, leads to difficulty in epithelialization by keratinocytes, developing a fibrotic scar, with less elasticity than the original skin, which may have disorders in predisposed individuals, resulting in hypertrophic scar and keloids. Biomedical materials have excellent characteristics, such as good biocompatibility and low immunogenicity, which can temporarily replace traditional materials used as primary dressings. In this work, we developed two dermal matrices based on Nile tilapia collagen, with (M_GAG) and without (M) glycosaminoglycans, using a sugarcane polymer membrane as a matrix support. To assess the molecular mechanisms driving wound healing, we performed qualitative proteomic analysis on the wound bed in an in vivo study involving immunocompetent murine models at 14 and 21 days post-full-thickness skin injury. Gene Ontology and Pathway analysis revealed that both skins were markedly represented by modulation of the immune system, emphasizing controlling the acute inflammation response at 14 and 21 days post-injury. Furthermore, both groups showed significant enrichment of pathways related to RNA and protein metabolism, suggesting an increase in protein synthesis required for tissue repair and proper wound closure. Other pathways, such as keratinization and vitamin D3 metabolism, were also enriched in the groups treated with M matrix. Finally, both matrices improved wound healing in a full post-thick skin lesion. However, our preliminary molecular data reveals that the collagen-mediated healing matrix lacking glycosaminoglycan (M) exhibited a phenotype more favorable to tissue repair, making it more suitable for use before skin grafts.

Shrimp laccase degrades polycyclic aromatic hydrocarbons from an oil spill disaster in Brazil: A tool for marine environmental bioremediation.

Our work aims to purify, characterize and evaluate a laccase from by-products of the shrimp farming industry (Litopenaeus vannamei) for the degradation of Polycyclic Aromatic Hydrocarbons (PAHs) from 2019 oil spill in Brazilian coast. The enzyme was purified by affinity chromatography and characterized as thermostable, with activity above 90 °C and at alkaline pH. In addition, the laccase was also tolerant to copper, lead, cadmium, zinc, arsenic, hexane and methanol, with significant enzymatic activation in acetone and 10 mM mercury. Concerning PAHs' degradation, the enzyme degraded 42.40 % of the total compounds, degrading >50 % of fluorene, C4-naphthalenes, C3-naphthalenes, C2-naphthalenes, anthracene, acenaphthene, 1-methylnaphthalene and 2-methylnaphthalene. Thus, this laccase demonstrated important characteristics for bioremediation of marine environments contaminated by crude oil spills, representing a viable and ecological alternative for these purposes.

Space-environment relationship in the identification of potential areas of expansion of Trypanosoma cruzi infection in Didelphis aurita in the Atlantic Rainforest.

Ecological Niche Modeling is widely used for animals, but rarely for understanding the parasite ecology. Trypanosoma cruzi is a heterogeneous and widely dispersed multi-host parasite. Didelphis aurita is a generalist species, both in terms of diet and environments. We modeled the D. aurita niche and T. cruzi infection in the Brazilian Atlantic Rainforest, using the models of two common vector species (Triatoma vitticeps and Panstrongylus megistus) as biotic variables, predicting their occurrence. Records of T. cruzi infected and non-infected D. aurita were analyzed through climate and landscape approaches by the Ecoland method. Models for each triatomine species and infected and noninfected D. aurita were produced considering climate and landscape: resolution of ~1km2 selected by Pearson's correlation [-0.7≤α≤0.7]. For modeling, seven algorithms available in ModleR package were used. True Skill Statistic was used to evaluate the models' performance (≥ 0.7). T. vitticeps indicates that there is a spatial dependence with warm areas in the southeastern region while P. megistus presented a distribution with high environmental suitability concentrated in the Southeast. High values of climatic suitability, landscape and potential presence of T. vitticeps and P. megistus were considered necessary, but not sufficient for the presence of D. aurita infected by T. cruzi. Climate models showed an ecological niche with suitability variations homogeneous, and landscape models showed a distribution of habitat conditions along the biome, with a fragmented profile and heterogeneous between locations. Ecoland demonstrated that D. aurita has different degrees of impact on its role in the enzootic cycle in different locations of the Atlantic Rainforest. Associating the models with the Ecoland method allowed the recognition of areas where D. aurita are important T. cruzi reservoirs. Areas of high suitability for the presence of marsupials are a necessary, but not sufficient for D. aurita to act as a reservoir for T. cruzi.

Trajetórias juvenis e a construção de intelectuais orgânicos/as , / Young people's trajectories and the construction of organic intellectuals ,

Resumo A partir do acompanhamento de uma trajetória de vida juvenil, buscou-se apreender de que forma e quais experiências colaboram para constituir jovens mais ativos socialmente - em uma perspectiva gramsciniana: intelectuais orgânicos/as. Questiona-se, portanto: quais seriam as experiências que incidem/incidiram sobre as vidas juvenis que contribuem/contribuíram para a construção de processos na direção da emancipação social? Assim, utilizou-se do acompanhamento singular e territorial — tecnologia social descrita e elaborada pela terapia ocupacional social — resultando nas descrições e análises de situações para que uma jovem engendrasse movimentos de catarse, destacando sua participação em ações de extensão universitária, ambientes virtuais, grêmio estudantil e atividades vinculadas ao programa de iniciação científica do ensino médio (PIBIC-EM). Projeta-se que este estudo ofereça referências para estratégias de fortalecimento das redes de jovens e fomento a criação e ampliação de diferentes espaços coletivos por onde os jovens transitam, sejam eles presenciais ou virtuais, de tal modo que possam enfrentar as diferentes vulnerabilidades que cercam seus cotidianos.

Abstract Based on the follow-up of a young woman, we sought to understand how and what experiences contribute to the formation of more socially active young people - from a Gramscian perspective, organic intellectuals. The question is, therefore, which experiences have or have had an influence on the lives of young people that contribute or have contributed to constructing processes towards social emancipation? To this end, an individual territorial follow-up—a social technology developed by social occupational therapy—was used, resulting in descriptions and analyses of situations so that this young woman could engender movements of catharsis, highlighting her participation and protagonism in university extension actions, virtual environments, student guild events, and activities linked to a high school research program. It is expected that this study will provide references for strategies to strengthen youth networks and foster the creation and expansion of different collective spaces where young people move about, whether in person or virtually so that they can cope with the vulnerabilities that surround their everyday life.

Hiperplasia fibroepitelial mamária em um gato macho: relato de caso / Mammary fibroepithelial hyperplasia in a male cat: case report

As neoplasias mamárias representam a terceira neoplasia mais comum em gatas, enquanto nos machos a incidência é rara. Esse trabalho relata o caso de um gato macho, não castrado, atendido no Hospital Veterinário da Universidade Estadual do Ceará, com uma neoformação mamária. O diagnóstico inicial, obtido por meio de punção aspirativa por agulha fina, sugeriu a presença de carcinoma mamário. Exames de imagem como a radiografia torácica e a ultrassonografia abdominal não evidenciaram a presença de metástase no parênquima pulmonar e nos órgãos abdominais, respectivamente. O tratamento instituído foi a remoção cirúrgica da neoformação, pela técnica de mastectomia parcial bilateral. O exame histopatológico da massa evidenciou uma hiperplasia fibroepitelial. Diante disso, conclui-se que, apesar de raras, as neoformações mamárias em gatos do sexo masculino podem ocorrer, devendo, dessa forma, compor parte da lista de diagnósticos diferenciais para aumentos de volume na região ventral desses animais.(AU)

Mammary neoplasms represent the third most common neoplasm in queens, while in tomcats the incidence is rare. This study reports the case of a tomcat, not neutered, treated at the Veterinary Hospital of the State University of Ceará, with a mammary neoformation. The initial diagnosis, obtained through aspiration cytology, suggested the presence of mammary carcinoma. Imaging tests such as thoracic radiography and abdominal ultrasound, did not show the presence of metastasis in the lung parenchyma and abdominal organs, respectively. The chosen treatment was the surgical removal of the neoformation, using the bilateral partial mastectomy technique. Histopathological examination of the mass showed a mammary fibroepithelial hyperplasia. Therefore, it is concluded that, although rare, mammary neoformations in male cats can occur, thus, it should be part of the list of differential diagnoses for increases in volume in the ventral region of these animals.(AU)

The influence of mat Pilates training adherence on strength and flexibility parameters in breast cancer survivors undergoing hormone therapy.

The aim of this study was to evaluate the influence of adherence to mat Pilates training on lower and upper body strength and flexibility in breast cancer survivors. Breast cancer survivors (≥40 years) with cancer stages 0-III undergoing hormone therapy participated in this study. For this secondary investigation only the intervention group was analyzed, divided into low and high training adherence. Participants performed a 60-min session of mat Pilates, 3 times/wk, for 24 weeks. Concentric, eccentric, and isometric hip flexor-extensor muscle peak torque, and isometric maximal strength parameters of the shoulder abductors, trunk extensors, and handgrip were assessed. Physical activity level was analyzed as a control variable. The results showed that high training adherence improved (P<0.05) left shoulder abductor strength parameters and lower and upper body flexibility compared to baseline. The low training adherence group improved (P<0.05) trunk extensors, right and left shoulder abductor strength parameters, handgrip strength, and extensor-flexor peak torque compared to baseline. There were no differences (P>0.05) between high and low adherence for physical activity level before and after the intervention. Therefore, it appears that higher training adherence most influences some strength parameters and flexibility, while fewer sessions enable the achievement of significant results for shoulder abductor and hip extensor-flexor muscle strength parameters.

Effects of Feeding Frequency for Nile Tilapia Fingerlings (Oreochromis niloticus).

This study is aimed at assessing the feeding frequency for tilapia fingerlings. 240 fishes were randomly distributed in 24 containers. The feeding consisted of six frequencies: 4 (F4), 5 (F5), 6 (F6), 7 (F7), 8 (F8), and 9 (F9) times a day. The weight gain was significantly higher in F5 and F6 when compared to F4 (p = 0.0409 and 0.0306, respectively). Feed intake and apparent feed conversion did not differ between treatments (p = 0.129 and p = 0.451). About water quality, the nitrogen levels in the water from the treatments differed between F4 and F5 (p = 0.0478) and F4 and F6 (p = 0.0283) and for P between F4 and F6 (p = 0.0215) and F4 and F9 (p = 0.0432). The x 2 test showed a dependence relationship between feed frequencies and the muscle fibers frequency (p < 22 × 10-17), prevailing fibers between 10-20 micrometers in F4, F5, F6, and F7 and 30-40 in F8 and F9. The area of the hepatocytes differed only between F5 and F9, while the nucleus area did not. Partial net revenue differed in 10% between F5 and F4 (p = 0.0812) and between F6 and F4 (p = 0.0568). In conclusion, fingerlings fed 5 to 6 times a day have better zootechnical and partial recipes.

Binding Mechanism between Acetylcholinesterase and Drugs Pazopanib and Lapatinib: Biochemical and Biophysical Studies.

Tyrosine kinase inhibitors (TKIs) are antitumor compounds that prevent the phosphorylation of proteins in a biological environment. However, the multitarget performance of TKIs promotes them as possible candidates for drug repositioning. In this work, interaction and inhibition studies through spectroscopic and computational techniques to evaluate the binding effectiveness of lapatinib and pazopanib TKIs to acetylcholinesterase (AChE) are reported. The results indicated potent inhibition at the µM level. The types of inhibition were identified, with pazopanib acting through non-competitive inhibition and lapatinib through acompetitive inhibition. The fluorescence suppression studies indicate a static mechanism for lapatinib-AChE and pazopanib-AChE systems, with a binding constant in the order of 105 M-1. The obtained thermodynamic parameters reveal interactions driven by van der Waals forces and hydrogen bonds in the lapatinib-AChE system (ΔH° and ΔS° < 0). In contrast, the pazopanib-AChE system shows positive ΔH° and ΔS°, characteristic of hydrophobic interactions. The Foster resonance energy transfer study supports the fluorescence studies performed. The 3D fluorescence studies suggest changes in the microenvironment of the tryptophan and tyrosine residues of the protein in contact with lapatinib and pazopanib. The results suggest effective inhibition and moderate interaction of the drugs with AChE, making them interesting for conducting more in-depth repositioning studies as AChE inhibitors.

Non-clinical repeated dose 28-day oral toxicity, reproductive toxicity and cytotoxicity studies of the polar fraction of Parkinsonia aculeata aerial parts extract.

This study was aimed to evaluate toxicity in repeated doses for 28 days, reproductive toxicity and cytotoxicity of a polar fraction obtained from the hydroethanolic extract of Parkinsonia aculeata (PfrHEPA) in experimental models. To perform the toxicity test in repeated doses for 28 days, male and female Wistar rats were treated via orogastric for 28 days with PfrHEPA (35, 70 or 140 mg/kg) according to the guidelines established by the Organisation for Economic Co-operation and Development (OECD) number 407 (1995). For assessment, the impact of PfrHEPA on the reproductive output various parameters were measured, including maternal weight, no. of pregnant females, female fertility index (%), gestation lengthtime, implantation sites, litter size and placental index of test animals. The cytotoxicity of PfrHEPA was performed on the tumor lines NCI-H292 (human lung carcinoma), HL-60 (human promyelocytic leukemia) and HCT-116 (colorectal cancer). In the repeated dose toxicity test for 28 days, no mortality was observed in the male and female rats treated with PfrHEPA as well as morphological changes and biochemical and hematological parameters. In the reproductive toxicity test, no abnormalities were observed related to the toxicological parameters in both mothers and offspring. Regarding the cytotoxicity assay, the PfrHEPA fraction did not demonstrate significant cytotoxic effect on the cell lines analyzed. The present results suggest the use of PfrHEPA is safe and well tolerated in rats. Further studies are planned to identify and purify the active compounds for subsequent in vivo evaluation.

A Hierarchical Grid Solver for Simulation of Flows of Complex Fluids.

Tree-based grids bring the advantage of using fast Cartesian discretizations, such as finite differences, and the flexibility and accuracy of local mesh refinement. The main challenge is how to adapt the discretization stencil near the interfaces between grid elements of different sizes, which is usually solved by local high-order geometrical interpolations. Most methods usually avoid this by limiting the mesh configuration (usually to graded quadtree/octree grids), reducing the number of cases to be treated locally. In this work, we employ a moving least squares meshless interpolation technique, allowing for more complex mesh configurations, still keeping the overall order of accuracy. This technique was implemented in the HiG-Flow code to simulate Newtonian, generalized Newtonian and viscoelastic fluids flows. Numerical tests and application to viscoelastic fluid flow simulations were performed to illustrate the flexibility and robustness of this new approach.

Concepções e práticas da vigilância em saúde no Rio Grande do Norte, Brasil: a voz dos gestores / Concepciones y prácticas de vigilancia en salud en Rio Grande do Norte, Brasil: la voz de los gerentes / Conceptions and practices of health surveillance in Rio Grande do Norte, Brazil: The voice of managers

Objetivo: compreender as concepções e as práticas da vigilância em saúde sob a ótica de seus gestores. Materiais e métodos: estudo qualitativo com a participação de 11 gestores estaduais e municipais do Rio Grande do Norte, Brasil, a partir de entrevistas semiestruturadas. Os dados foram construídos com apoio do programa Iramuteq, e a análise foi feita pela técnica de análise temática de conteúdo. Resultados: os gestores compreenderam a vigilância em saúde como um modelo indispensável para o Sistema Único de Saúde brasileiro. Encontraram-se contradições no modo de fazer das ações desenvolvidas e na efetividade da articulação intra e intersetorial. Destacam-se a precariedade das condições de trabalho, a insuficiência de recursos humanos e as fragilidades principalmente no setor da saúde do trabalhador. Conclusões: a compreensão sobre os conceitos e a missão da vigilância em saúde apresentou incoerências internas nos discursos sobre a efetivação do planejamento coletivo e das ações entre as diversas vigilâncias. Aponta-se a necessidade de repensar o itinerário formativo dos gestores na interação ensino-serviço para a transformação da prática.

Objetivo: comprender las concepciones y prácticas de la vigilancia en salud desde la perspectiva de sus gestores. Materiales y métodos: estudio cualitativo con la participación de once gestores estatales y municipales en Rio Grande do Norte, Brasil, a través de entrevistas semiestructuradas. Los datos se recolectaron con el apoyo del programa Iramuteq y fueron analizados con la metodología de análisis de contenido. Resultados: los participantes consideran a la vigilancia en salud como un modelo indispensable para el Sistema Único de Salud brasileño. No obstante, se identificaron contradicciones en la forma de implementar ciertas acciones y en la efectividad de la articulación intra e intersectorial. Así mismo, se identificó precariedad en las condiciones de trabajo, falta de recursos humanos y debilidades en el área de la salud ocupacional. Conclusiones: la comprensión de los conceptos y de la misión de la vigilancia en salud presentó inconsistencias internas en cuanto a los discursos sobre la efectividad de la planeación colectiva y las acciones entre las diversas dependencias a cargo de esta labor. Se señala la necesidad de replantear la agenda formativa de los gestores en la interacción enseñanza-servicio para la transformación de la práctica de vigilancia en salud.

Objective: To understand the conceptions and practices around health surveillance from the perspective of managers in charge of this process. Materials and methods: Qualitative study carried out with eleven health surveillance managers of Rio Grande do Norte, Brazil, using semi-structured interviews as data collection technique. Iramuteq software helped with the data collection process, while the thematic content analysis technique was used for its analysis. Results: Participants consider health surveillance as an essential model within the Brazilian health system. However, contradictions were found in the way of deploying the actions developed and on the effectiveness of intra and intersectoral articulation. Reports on the precariousness of working conditions, the lack of human resources, and weaknesses in the occupational health sector, particularly, were also identified. Conclusions: The understanding of the concepts and mission of health surveillance has internal inconsistencies in the discourses about the implementation of collective planning and actions among the various areas in charge of this process. Rethinking the formative agenda of managers in the teachingservice interaction is required for the transformation of health surveillance practice.

Perfil epidemiológico de Staphylococcus spp. isolados de hemoculturas de pacientes internados em um hospital de atenção terciária da rede pública do Ceará / Epidemiological profile of Staphylococcus spp. isolated from hemocultures of interned patients in a tertiary attention hospital of the Ceará public network

Staphylococcus spp. vem ganhando destaque em infecções na corrente sanguínea (ICS), apresentando alta prevalência, multirresistência e considerável poder de letalidade. O presente estudo teve como objetivo analisar a prevalência e traçar o perfil de sensibilidade das espécies de Staphylococcus spp. isoladas de amostras de hemoculturas positivas obtidas de um hospital de atenção terciária da rede pública do Ceará, no período de janeiro de 2015 a dezembro de 2018. Dos 3292 exames de hemocultura realizados, apenas 15,88% tiveram resultado positivo, dos quais 24,85% eram cocos Gram positivos. S. aureus representou 1,53% das hemoculturas positivas com 50% das cepas resistentes à oxacilina. Os isolados de Staphylococcus sp. coagulase negativo obtiveram prevalência de 10,89%, representados por: S. epidermidis (n=23), S. haemolyticus (n=17), S. hominis (n=13), S. saprophyticus (n=2) e S. warneri (n=2). Verificou-se multirresistência em diversos isolados analisados, apresentando variações intra e interespécies. Portanto, nossos achados melhoram o entendimento da epidemiologia das ICS causadas por Staphylococcus spp., na instituição de estudo, bem como seu perfil de sensibilidade. A identificação precoce do agente infeccioso auxilia na escolha adequada do tratamento, aumentando as chances de cura e reduzindo o tempo de internação do paciente. (AU)

Staphylococcus spp. has been highlighted among bloodstream infections (BI), presenting high prevalence, multidrug resistance and considerable lethality. The present study aimed to analyze the prevalence and the susceptibility profile of Staphylococcus spp. isolated from positive blood cultures from a tertiary care public hospital of Ceará, from January 2015 to December 2018. Of the 3292 blood cultures performed during this period, only 15.88% were positive, of which 24.85% were Gram positive cocci. S. aureus represented 1.53% of positive blood cultures, of which 50% were oxacilin resistant. Isolates of coagulase-negative Staphylococcus spp. showed a prevalence of 10.89%, represented by: S. epidermidis (n = 23), S. haemolyticus (n = 17), S. hominis (n = 13), S. saprophyticus (n = 2) and S. warneri (n = 2). Multiresistance occurrence was verified in several of the analyzed isolates, presenting intra and inters species variations. Therefore, our findings improve the understanding of the epidemiology of BI caused by Staphylococcus spp. in the studied institution, as well as its susceptibility profile. Early identification of the infectious agent might aid in the appropriate choice of treatment, increasing the chance of cure and reducing the patient length stay in hospital. (AU)

Evaluación de la precisión de impresiones sobre implantes usando guía multifuncional en el sistema all-on-four / Accuracy of implant impression using a multifunctional guide in the All-on-Four treatment concept

ABSTRACT Introduction: The passivity of prosthetic components is one of the most important prerequisites in oral implant rehabilitation for maintenance of osseointegration. Objective: Thus, the present study analyzed in vitro the accuracy of different molding techniques in prosthetic rehabilitation on angled implants installed in an anatomical model of a metallic edentulous maxilla. Methods: Laboratory experiment study. A reference metal model of an edentulous maxilla was used. A metal framework was used for the misadaptation assessment. Three groups (n= 10) were compared and impressions were made with vinyl-polysiloxane and casts were obtained with type IV stone: (1) Impression with a metallic open-tray without splinted transfers; (2) Impression with a metallic open-tray and metal splinted transfers; (3) Impression with a multifunctional guide and metal splinted transfers. Misadaptation was assessed with the aid of a stereomicroscope and measuring software. Data were submitted to ANOVA with Welch correction and the Games-Howell post-hoc test with the significance set at 5 percent. Results: Misadaptation (µm) was 110,23 ± 30,94, 37,53 ± 3,92, and 37,69 ± 2,79 for the the groups. Statistically significant differences between impression with a metallic open-tray with and without splinted transfers were observed (p < 0,001). No significant differences between the other types of impression. Conclusions: Impression with a metallic open-tray without splinted transfers and with a multifunctional guide splinted with metal transfers were the most precise methods resulting in higher accuracy in transferred implants(AU)

RESUMEN Introducción: La pasividad de los componentes protésicos es uno de los prerrequisitos más importantes para el mantenimiento de la osteointegración en la rehabilitación oral sobre implantes. Objetivo: Evaluar in vitro la precisión de diferentes técnicas de impresión en rehabilitación sobre implantes inclinados instalados en un modelo anatómico de un maxilar metálico desdentado. Métodos: Estudio de laboratorio de tipo experimental. Se utilizó un modelo metálico de referencia de un maxilar desdentado. Fue utilizada una barra metálica para la evaluación de la desadaptación. Se compararon tres grupos (n = 10), se realizaron impresiones con polivinilsiloxano y se obtuvieron modelos con yeso piedra tipo IV: (1) impresión con una cubeta metálica abierta sin unión de los transferentes; (2) impresión con una cubeta metálica abierta y unión de los transferentes con cilindros metálicos; (3) impresión con una guía multifuncional y unión de los transferentes con cilindros metálicos. La desadaptación se evaluó con la ayuda de un microscopio estereoscópico y un software de medición. Los datos se enviaron a ANOVA con la corrección de Welch y la prueba post-hoc de Games-Howell con la significación establecida en 5 por ciento. Resultados: La desadaptación (µm) fue de 110,23 ± 30,94; 37,53 ± 3,92 y 37,69 ± 2,79 para los grupos, respectivamente. Se observaron diferencias estadísticamente significativas entre la impresión con cubeta abierta metálica con y sin unión de los transferentes (p <0,001). No hubo diferencias significativas entre los otros tipos de impresiones. Conclusiones: La impresión con cubeta abierta metálica sin unión de los transferentes y con una guía multifuncional con unión de los transferentes con cilindros metálicos fueron los métodos más precisos que dieron como resultado una mayor precisión en la transferencia de la posición de los implantes(AU)

Effects of Concurrent Training with Elastic Tubes in Hypertensive Patients: A Blind Controlled Randomized Clinical Trial.

BACKGROUND: Concurrent training (CT) has been recommended to minimize the deleterious effects of aging. However, few studies have investigated whether this type of training reduces blood pressure in the elderly. Therefore, our objective was to evaluate the effects of CT on the hemodynamic, cardiorespiratory, and muscle strength responses in medicated hypertensive patients. METHODS: Twenty-three hypertensives (62.65 ± 6.4 years) of both sexes were allocated to the concurrent training group (CTG) or control group (CG). The CTG performed aerobic training (70-85% of reserve heart rate) combined with resistance training with elastic tubes (2sets × 15 repetitions) for 8 weeks. Resting blood pressure (BP), peak oxygen consumption (VO2peak), and right knee and elbow flexion strength were evaluated. RESULTS: A reduction of 6.37% was observed in BP and increases of 16.68% in VO2peak and 16% in muscle strength for right elbow flexion in the CTG compared to CG (p < .05). Intragroup comparisons showed reduction of 5% for BP, and increases of 6.79% for VO2peak, 24.79% for elbow flexion, and 16.47% for knee flexion in the CTG (p < .05), without significant improvement in the CG. CONCLUSION: CT promoted a reduction in BP, and increased cardiorespiratory fitness and muscular strength of the upper limbs in the hypertensive older adults.

Use of alveolar distraction osteogenesis for anterior maxillary defect reconstruction.

Alveolar osteogenic distraction (AOD) is a biological process through which new bone formation occurs between bone segments that are gradually separated by incremental traction. This case report described the oral rehabilitation with dental implants of a patient with a vertical bone defect in the maxillary anterior region using the AOD technique. The patient presented with absence of the teeth 22, 21, 11, and 12 associated with a vertical bone defect. The AOD was performed using a supported osteodistractor device surgically installed with subsequent daily activations. After 21 days, the ideal positioning of bone fragment was confirmed and activation was ceased. Five months after the initial surgery, two dental implants were installed in the region of teeth 12 and 22. An FP3 metal-ceramic prosthesis was installed offering satisfactory esthetic results. In conclusion, the use of AOD to increase the alveolar ridge was effective and ensured rehabilitation with dental implants.

Análise da associação dos aspectos clínicos, histopatológicos e terapêuticos em crianças e adolescentes com osteossarcoma / Analysis of the association of clinical, histopathological e therapeutic aspects, in children and teenagers with osteosarcoma

Introdução: O osteossarcoma é um tumor maligno raro, tipo mais comum de câncer ósseo, com maior frequência em crianças e adolescentes. É um tumor agressivo que evolue rapidamente, porém quando diagnósticado precocemente aumenta as chances de cura e, por conta da evolução das técnicas cirurgicas e de novas drogas quimioterapicas, vem a permitir um tratamento mais especifico. Objetivo: Avaliar, no osteossarcoma, a associação entre as caracteristicas sociodemográficas, clínicas-patológicas e terapêuticas em crianças e adolescentes com a evolução da doença. Metodologia: Estudo de coorte retrospectivo entre os anos de 2010 a 2017, onde foram incluidos um total de 64 pacientes com osteossarcoma de ambos os sexos com idade ≤ 21 anos. Os dados foram provenientes de prontuários e para a análise estatísticas de associação foram utilizados os testes exato de Fischer e Qui-quadrado, sendo considerado significativo p<0,05. A estimativa do tempo de sobrevida foi realizada pela curva de Kaplan-Meier e teste de Log-rank. Resultados: A mediana da idade foi 14 anos, a maioria do sexo masculino (57,8%) e raça não branca (89,1%). No sexo feminino, houve uma maior frequência de tumor no fêmur (66,7%), de localização distal (66,7%) e tamanho menor que 15 cm (85,2%) quando comparado ao grupo masculino (p=0,041; p=0,043; p=0,025, respectivamente). No sexo masculino, houve uma maior frequência de óbito (59,5%; p=0,023). Na faixa etária <13, a quimioterapia metronômica foi realizada em 79,7% dos casos (p<0,0001). No grupo com tamanho tumoral ≥ 15, obteve uma maior frequência de estádio III (94,4%), cirurgias com amputação (72,0%), quimioterapia metronômica (70,8%), metástases e óbitos (72,0%) (p<0,05). O grupo com aparecimento de metástases, verificou-se uma maior frequência de recidiva local (30,0%). A mediana de sobrevida global (SG) foi 4,08 anos dos paciente com o mínimo de 0,15 anos e máximo de 9,55 anos. A mediana de SG no grupo do sexo masculino foi de 2,5 anos com taxa de SG de 50,6%, e no sexo feminino foi de 73,2% (p=0,0188). A mediana de SG no grupo com tumor < 15 cm foi de 1,86 anos com taxa de SG de 83,6% e de 46,6% no grupo com tumores ≥ 15 cm (p=0,0002). Conclusão: No osteossarcoma, os principais fatores associados ao prognóstico desfavorável foram ser do sexo masculino e ter tamanho tumoral acima de 15 cm (AU)

Introduction: Osteosarcoma is a rare malignant tumor and the most prevalent bone malignant tumor in children and teenagers. They are agressive and grow rapidly but when diagnosed early, chances of cure raise and, due to appropriate polichemotherapy and surgical management limb salvage is more frequent than amputation. Objective: Analize the clinical follow up of pediatric patients with osteosarcoma treated at the Cancer Hospital of Pernambuco.Methods: This is a retrospective cohort study where 64 patients with osteosarcoma of both genders were included. Age varied from > 4 to _< 21 years old during the period from 2010 to 2017. All data were collected from patients hospital files and aubmitted to Fischer and Chi Square tests, being considered significative a p<0,05. The overall survival and free disease survival rates were represented by a Kaplan-Meyer curve and compared to the Log Rank. For the analysis of death association, the Hazard ratio was applyed. Results: the median age was 14 and the majority of males (57,8%) and non white. Most came from the countryside of Pernambuco and neighbor states (68,8%) and Recife and its metropolitan area (31,3%). Analysing the clinical aspects, the most frequent sympton was a growing mass (40,6%) and intensive pain (34,4%). The histologycal subtype was the osteoblastic (77,1%) and most frequent tumor site was the femur (p=0,041) distally (p=0,043) and size <15cm (p=0,025) all significative and associated to the female gender. In the association survival status and gender, the male one was significative to death (p=0,023). In relation to TNM staging the most prevalent was stage III (73,4%) being significative to tumors <15cm (p=0,040) and Enneking stage most prevalent was IIB (90,5%). Significane was observed in the association of tumors <15cm, limb salvage surgery (p=0,037), metronomic polichemotherapy (p=0,024), overall survival (p=0,004) and metastasis (p=0,004). Follow up with metastasis during treatment was observed in 46,9% of all cases and associated to local recurrence (p=0,005) being the lungs the most frequent site (76,7%). The treatment aplyed was polichemotherapy (95,3%), surgery (96,9%) being 54,9% amputation and radiotherapy 1,6%. Metronomic chemotherapy was used in 79,7% of all patients showing significancy for the age ranging from >4 to <13 (p=0,0001). Local recurrence ocurred in 15,9% of cases and among them 50% died. The overall survival median was of 4,08 years with a minimum of 0,15 years and free overall survival and disease progression was greater for those using methrotexate in high doses. Conclusion: It was conclude that tumors < 15cm, female gender and a metronomic polichemotherapy were positive prognostic factors for osteosarcoma and overall survival. The male gender showed to be a negative risk factor leading to death (AU)

Characterization of brain acetylcholinesterase of bentonic fish Hoplosternum littorale: Perspectives of application in pesticides and metal ions biomonitoring.

Acetylcholinesterase (AChE; EC 3.1.1.7) is a serine hydrolase, whose main function is to modulate neurotransmission at cholinergic synapses. It is, therefore, the primary target of some pesticides and heavy metals. Its inhibition in aquatic organisms has been used as an indicator of the presence of these pollutants in water bodies. The present study aimed to characterize physicochemical and kinetic parameters of brain AChE in the benthic fish Hoplosternum littorale and to analyze the in vitro effects of pesticides (dichlorvos, diazinon, chlorpyrifos, parathion-methyl, temephos, carbaryl, carbofuran, aldicarb, diflubenzuron, novaluron and pyriproxyfen) and metal ions (As3+, Cd2+, Cu2+, Fe2+, Mn2+, Mg2+, K+, Pb2+, Hg2+, Zn2+) investigating the potential of this enzyme as environmental biomarker based on current regulations. Specific substrates and inhibitors have indicated AChE to be the predominant cholinesterase (ChE) in the brain of H. littorale. Peak activity was observed at pH 8.0 and 30 °C. The enzymatic activity is otherwise moderately thermostable (≈ 50% activity at 45 °C). The enzyme can reduce the activation energy of acetylthiocholine hydrolysis reaction to 8.34 kcal mol-1 while reaching a rate enhancement of 106. Among the pesticides under study, dichlorvos presented an IC50 value below the maximum concentrations allowed by legislation. This study presents the first report on the inhibition of brain AChE activity from Siluriformes by the pesticides novaluron and pyriproxyfen. Mercury ion also exerted a strong inhibitory effect on its enzymatic activity. The H. littorale enzyme thus has the potential to function as an in vitro biomarker for the presence of the pesticide dichlorvos as well as mercury in areas of mining and industrial discharge.

Functional resistance training can increase strength, knee torque ratio, and functional performance in elderly women.

Functional resistance training can increase strength, knee torque ratio, and functional performance in elderly women. The aim of the present study was to understand the effects of closed kinetic chain exercises with constant load on muscular strength, the knee torque conventional ratio (hamstring:quadriceps - H:Q), and functional capacity in the elderly. Nine untrained healthy elderly women participated in experimental resistance training. Ten-repetition maximum (10RM) for the deadlift, isokinetic maximum voluntary concentric contraction, and functional capacity were assessed before and after the 7-week resistance-training program. Magnitude based inference analysis was used to examine the differences in muscle strength and functional performance. Therefore, the smallest worthwhile change was calculated and 90% confidence intervals were also determined to characterize muscle strength and functional performance. The analysis demonstrated an increase in the deadlift and knee flexor torque (60o/sec) after the experimental intervention. Furthermore, stair ascent, knee extensor torque (120o/sec), knee flexor torque (120o/sec), and knee ratio (60o/sec) also presented a positive effect in the same training period. Conversely, knee extensor torque (60o/sec), and knee ratio (120o/sec) did not show conclusive responses. In conclusion, resistance training with functional closed kinetic chain exercises and constant volume load (i.e., 65% of 1-repetition maximum) can increase deadlift 10RM, isokinetic concentric torque, the conventional H:Q ratio, and stair ascent performance in elderly women.

Acetylcholinesterase of mangrove oyster Crassostrea rhizophorae: A highly thermostable enzyme with promising features for estuarine biomonitoring.

Enzyme biomarkers from several aquatic organisms have been used for assessing the exposure to contaminants at sublethal levels. Amongst them, the cholinesterases are commonly extracted from several organisms to evaluate/measure organophosphate and carbamate neurotoxic effects. Acetylcholinesterase (AChE; EC 3.1.1.7) is an enzyme of the group of serine esterases that acts on the hydrolysis of the neurotransmitter acetylcholine allowing the intermittence of the nerve impulses responsible for the neuronal communication. This enzyme is the main target for the action of some pesticides and the inhibition of its activity in bivalve mollusks may be used as biomarker due to their filter-feeding habit. In this context, the present study aimed to characterize physicochemical and kinetic parameters of the AChE extracted from gills and viscera of the oyster Crassostrea rhizophorae and investigate the in vitro effect of pesticides (dichlorvos, diazinon, chlorpyrifos, methyl-parathion, temephos, carbaryl, carbofuran, aldicarb, diflubenzuron and novaluron) in search for assessing its potential as biomarker. Specific substrates and inhibitors evidenced the predominance of AChE in both tissues. The optimum pH found for gills and viscera AChE were 8.0 and 8.5, respectively. The maximum peak of activity occurred at 70 °C for gill AChE and 75 °C for viscera AChE. The enzymes of both tissues presented remarkable thermostability. The Michaelis-Menten constant for both enzymes were 1.32 ±â€¯0.20 mM for gills and 0.43 ±â€¯0.12 mM for viscera. The Vmax values for gills and viscera were 53.57 ±â€¯1.72 and 27.71 ±â€¯1.15 mU/mg, respectively. The enzymes were able to reduce the activation energy to 9.75 kcal mol-1 (gills) and 11.87 kcal mol-1 (viscera) obtaining rate enhancements of 3.57 × 105 and 1.01 × 104, respectively, in relation to non-catalyzed reactions. Among the pesticides under study, the carbamates carbaryl and carbofuran exerted the strongest inhibitory effects on the enzyme activity achieving important degrees of inhibition at concentrations below national and international current regulations. The first observation of the effects of benzoylurea pesticides (diflubenzuron and novaluron) on AChE from mollusks is reported here. The gills AChE of C. rhizophorae showed potential to be specific biomarker for the carbamate carbaryl while the viscera AChE showed it for carbofuran. According to their features, these enzymes may be proposed as promising tools for estuarine monitoring as well as biocomponent of biosensor devices.