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1.
J. nurs. health ; 10(4): 20104010, abr.2020.
Article in Portuguese | LILACS, BDENF - Nursing | ID: biblio-1097582

ABSTRACT

Objetivo: analisar na literatura as diferentes recomendações nacionais e internacionais sobre o uso de máscaras no ambiente hospitalar durante a pandemia ocasionada por Coronavírus. Método: reflexão teórica fundamentada em uma revisão bibliográfica de artigos e manuais sobre os temas "equipamento de proteção individual", "máscaras" "proteção pessoal" e "infecções por coronavirus". Resultados: diferentes tipos de máscaras vêm sendo empregadas como meio de proteção durante o enfrentamento da pandemia. Para sua utilização, os profissionais de saúde devem seguir boas práticas de uso, combinada as outras medidas de proteção. Conclusões: o vírus foi recentemente descoberto, protocolos são realizados e sofrem mudanças constantes. Existem lacunas referente às orientações e recomendações na literatura sobre o uso desses equipamentos de proteção durante uma pandemia desta magnitude.(AU)


Objective: to analyze in the literature the different national and international recommendations on the use of masks in the hospital environment during the pandemic caused by coronavirus. Method: theoretical reflection based on a bibliographic review of articles and manuals on the topics "personal protective equipment", "masks" "personal protection" and "coronavirus infections". Results: different types of masks have been used as a means of protection during the pandemic. For its use, health professionals must follow good usage practices, combined with other protective measures. Conclusions: the virus was recently discovered, protocols are carried out and undergo constant changes. There are gaps regarding the guidelines and recommendations in the literature on the use of such protective equipment during a pandemic of this magnitude.(AU)


Objetivo: analizar en la literatura las diferentes recomendaciones nacionales e internacionales sobre el uso de máscaras en el entorno hospitalario durante la pandemia causada por el coronavirus. Método: reflexión teórica basada en una revisión bibliográfica de artículos y manuales sobre los temas "equipo de protección personal", "máscaras", "protección personal" e "infecciones por coronavirus". Resultados: se han utilizado diferentes tipos de máscaras como medio de protección durante la pandemia. Para su uso, los profesionales de la salud deben seguir buenas prácticas de uso, combinadas con otras medidas de protección. Conclusiones: el virus fue descubierto recientemente, los protocolos se llevan a cabo y experimentan cambios constantes. Hay lagunas con respecto a las pautas y recomendaciones en la literatura sobre el uso de dicho equipo de protección durante una pandemia de esta magnitud.(AU)


Subject(s)
Humans , Coronavirus Infections , Personal Protection , Personal Protective Equipment , Masks
2.
J. nurs. health ; 10(4): 20104022, abr.2020.
Article in Portuguese | BDENF - Nursing, LILACS | ID: biblio-1129478

ABSTRACT

Objetivo: relatar a experiência da equipe interprofissional quanto às medidas de segurança adotadas frente a pandemia ocasionada pelo novo Coronavírus em um centro de oncologia e infusão no sul do Brasil. Método: relato de experiência da equipe interprofissional de oncologia relacionado às medidas adotadas entre os meses de março a abril de 2020 para manutenção das atividades de forma segura em meio a pandemia. Resultados: medidas de distanciamento, intensificação de higiene, redução de fluxo de pessoas e uso de equipamentos de proteção individual foram adotadas. Além destas, adequações na estrutura e fluxos operacionais do serviço foram implementados para otimização de novas práticas de atendimento que busquem minimizar a exposição do paciente ao vírus. Conclusões: ajustes nas práticas organizacionais e operacionais para um atendimento interprofissional organizado e seguro ao paciente em um centro de oncologia e infusão garante o atendimento na unidade e assegura o adequado tratamento aos pacientes.(AU)


Objective: to report the experience of the interprofessional team regarding the safety measures adopted in the face of the pandemic caused by the new Coronavirus in an oncology and infusion center in southern Brazil. Method: experience report of the interprofessional oncology team related to the measures adopted between the months of March to April 2020 to maintain activities safely in the middle of the pandemic. Results: distance measures, intensified hygiene, reduced flow of people and use of personal protective equipment were adopted. In addition to these, adjustments to the structure and operational flows of the service were implemented to optimize new care practices that seek to minimize patient exposure to the virus. Conclusions: adjustments in organizational and operational practices for organized and safe interprofessional care for patients in an oncology and infusion center guarantees care at the unit and ensures adequate treatment for patients.(AU)


Objetivo: informar la experiencia del equipo interprofesional sobre las medidas adoptadas ante la pandemia provocada por el nuevo Coronavirus en un centro de oncología e infusión en Sul del Brasil. Método: informe de experiencia del equipo interprofesional de oncología relacionado con las medidas adoptadas entre los meses de marzo a abril de 2020 para mantener las actividades de forma segura en medio de la pandemia. Resultados: se adoptaron medidas de distancia, higiene intensificada, flujo reducido de personas y uso de equipo de protección personal. Además de estos, se implementaron ajustes a la estructura y flujos operativos del servicio para optimizar nuevas prácticas de atención que buscan minimizar la exposición del paciente al virus. Conclusiones: los ajustes en las prácticas organizativas y operativas para la atención interprofesional organizada y segura de los pacientes en un centro de oncología e infusión asegura un tratamiento adecuado a los pacientes.(AU)


Subject(s)
Oncology Nursing , Coronavirus Infections , Pandemics , Patient Safety
3.
Rev. enferm. UFPE on line ; 14: [1-7], 2020.
Article in Portuguese | BDENF - Nursing | ID: biblio-1096024

ABSTRACT

Objetivo: conhecer como os enfermeiros atuam na avaliação e tratamento de lesões por pressão no contexto da Estratégia Saúde da Família. Método: trata-se de um estudo qualitativo, descritivo e exploratório em unidades urbanas da Estratégia Saúde da Família. Coletaram-se os dados por meio de entrevistas, com um instrumento semiestruturado aplicado a dez enfermeiros, sendo posteriormente analisados pela técnica de Análise de Conteúdo na modalidade Análise Categorial. Resultados: elencaram-se as seguintes categorias: Participação em formação específica sobre lesão por pressão; Aplicação de método de avaliação de lesões por pressão; Indicação, utilização e disponibilidade de coberturas para a prevenção e tratamento de lesões por pressão e Orientações a pacientes e familiares sobre os cuidados com lesões por pressão. Conclusão: conclui-se que o enfermeiro necessita possuir o conhecimento teóricoprático para que, juntamente à equipe multiprofissional e à família, se promova o cuidado na prevenção e tratamento necessário a estes pacientes.(AU)


Objective: to know how nurses work in the evaluation and treatment of pressure ulcers in the context of the Family Health Strategy. Method: this is a qualitative, descriptive and exploratory study in urban units of the Family Health Strategy. The data was collected through interviews, with a semi-structured instrument applied to ten nurses, being subsequently analyzed by the Content Analysis technique in the Category Analysis modality. Results: the following categories were listed: Participation in specific training on pressure ulcers; Application of pressure ulcer assessment method; Indication, use and availability of coverings for the prevention and treatment of pressure ulcers and Guidance to patients and family members on care for pressure ulcers. Conclusion: it is concluded that the nurse needs to have the theoretical-practical knowledge so that, together with the multiprofessional team and the family, the care in the prevention and treatment necessary for these patients is promoted.(AU)


Objetivo: saber cómo trabajan los enfermeros en la evaluación y el tratamiento de las lesiones por presión en el contexto de la Estrategia de Salud Familiar. Método: se trata de un estudio cualitativo, descriptivo y exploratorio en unidades urbanas de la Estrategia de Salud Familiar. Los datos fueron recolectados a través de entrevistas, con un instrumento semiestructurado aplicado a diez enfermeros, analizándolos posteriormente por la técnica de Análisis de Contenido en la modalidad de Análisis de Categoría. Resultados: se enumeraron las siguientes categorías: participación en capacitación específica sobre lesiones por presión; Aplicación del método de evaluación de lesiones por presión; Indicación, uso y disponibilidad de recubrimientos para la prevención y el tratamiento de lesiones por presión y orientación a pacientes y familiares sobre la atención de lesiones por presión. Conclusión: se concluye que el enfermero necesita tener el conocimiento teórico-práctico para que, junto con el equipo multiprofesional y la familia, se promueva la atención en la prevención y el tratamiento necesarios para estos pacientes.(AU)


Subject(s)
Humans , Male , Female , Adult , Middle Aged , National Health Strategies , Pressure Ulcer , Pressure Ulcer/nursing , Pressure Ulcer/prevention & control , Pressure Ulcer/therapy , Patient Safety , Nurses , Health Education , Epidemiology, Descriptive , Qualitative Research
4.
Nursing (Ed. bras., Impr.) ; 22(257): 3220-3225, out.2019.
Article in Portuguese | BDENF - Nursing, LILACS | ID: biblio-1026095

ABSTRACT

Objetivo: identificar a percepção dos enfermeiros de unidades de internação clínica sobre a importância da Sistematização da Assistência de Enfermagem para o planejamento da assistência. Método: Estudo descritivo, qualitativo, realizado após aprovação do comitê de ética em pesquisa, em um hospital universitário de Porto Alegre. Resultados: foram entrevistados 18 enfermeiros. A análise temática dos dados revelou as seguintes áreas: Sistematização da Assistência de Enfermagem: Conhecimento e Aplicação na prática profissional: dificuldades encontradas. Conclusão: apesar das comprovações científicas de sua eficiência, a implementação da SAE necessita superar as limitações impostas pela falta de compreensão do papel do enfermeiro em sua prática assistencial. O aprimoramento científico torna-se imperativo no desenvolvimento de atitudes e habilidades direcionadas a um cuidado ético e responsável.(AU)


Objective: identify the perception of the nurses of clinical hospitalization units about the importance of Nursing Care Systematization for care planning. Method: Descriptive, qualitative study, carried out after approval of the research ethics committee, at a university hospital in Porto Alegre. Results: 18 nurses were interviewed. The thematic analysis of the data revealed the following areas: Systematization of Nursing Assistance: Knowledge and Application in professional practice: difficulties encountered. Conclusion: despite the scientific evidence of its efficiency, the SAE implementation needs to overcome the limitations imposed by the lack of understanding of the role of nurses in their care practice. The scientific improvement becomes imperative in the development of attitudes and abilities directed towards an ethical and responsible care.(AU)


Objetivo: identificar la percepción de las enfermeras de las unidades de hospitalización clínica sobre la importancia de la sistematización de la atención de enfermería para la planificación de la atención. Método: Estudio descriptivo, cualitativo, realizado después de la aprobación del comité de ética de la investigación, en un hospital universitario de Porto Alegre. Resultados: se entrevistó a 18 enfermeras. El análisis temático de los datos reveló las siguientes áreas: Sistematización de la asistencia de enfermería: conocimiento y aplicación en la práctica profesional: dificultades encontradas. Conclusión: a pesar de la evidencia científica de su eficiencia, la implementación de SAE debe superar las limitaciones impuestas por la falta de comprensión del papel de las enfermeras en su práctica de atención. La mejora científica se vuelve imperativa en el desarrollo de actitudes y habilidades dirigidas hacia un cuidado ético y responsable.(AU)


Subject(s)
Humans , Patient Care Planning , Professional Practice , Nursing Care/methods , Nursing Process , Inpatient Care Units
5.
Hypertens Res ; 42(12): 1883-1893, 2019 12.
Article in English | MEDLINE | ID: mdl-31506648

ABSTRACT

The aim of this study was to investigate whether treatment with diminazene aceturate (DIZE), a putative ACE2 activator, or with angiotensin-(1-7) during pregnancy could attenuate the development of cardiovascular dysfunction in the adult offspring of spontaneously hypertensive rats (SHRs). For this, pregnant SHRs received DIZE or Ang-(1-7) throughout gestation. The systolic blood pressure (SBP) was measured in the male offspring from the 6th to16th weeks of age by tail-cuff plethysmography. Thereafter, the left ventricular contractile function and coronary reactivity were evaluated by the Langendorff technique. Samples of the left ventricles (LVs) and kidneys were collected for histology and western blot assay in another batch of adult rat offspring. Maternal treatment with DIZE or Ang-(1-7) during pregnancy attenuated the increase in SBP in adult offspring. In addition, both DIZE and Ang-(1-7) treatments reduced the cardiomyocyte diameter and fibrosis deposition in the LV, and treatment with Ang-(1-7) also reduced the fibrosis deposition in the kidneys. Maternal treatment with DIZE, as well as Ang-(1-7), improved the coronary vasodilation induced by bradykinin in isolated hearts from adult offspring. However, no difference was observed in the contractile function of the LVs of these animals. The expression levels of AT1 and Mas receptors, ACE, ACE2, SOD, and catalase in the LV were not modified by maternal treatment with Ang-(1-7), but this treatment elicited a reduction in AT2 expression. These data show that treatment with DIZE or Ang-(1-7) during gestation promoted beneficial effects of attenuating hypertension and cardiac remodeling in adult offspring.


Subject(s)
Angiotensin I/pharmacology , Cardiovascular Diseases/prevention & control , Diminazene/analogs & derivatives , Enzyme Activators/pharmacology , Hypertension, Pregnancy-Induced/drug therapy , Peptide Fragments/pharmacology , Peptidyl-Dipeptidase A/drug effects , Angiotensin-Converting Enzyme 2 , Animals , Blood Pressure/drug effects , Diminazene/pharmacology , Female , Heart/drug effects , Kidney/drug effects , Male , Myocardial Contraction , Pregnancy , Prenatal Exposure Delayed Effects , Rats , Ventricular Function, Left
6.
Nursing (Ed. bras., Impr.) ; 21(240): 2178-2183, maio 2018.
Article in Portuguese | LILACS, BDENF - Nursing | ID: biblio-907902

ABSTRACT

Objetivo: Investigar práticas de educação em saúde de enfermeiros na Estratégia de Saúde da Família voltadas para portadores de hipertensão e diabetes...


Objective: To investigate the health education practices of nurses in the Family Health Strategy aimed at patients with hypertension and diabetes...


Objetivo: Investigar prácticas de educación en salud de enfermeros en la Estrategia de Salud de la Familia dirigidas a portadores de hipertensión y diabetes...


Subject(s)
Humans , Diabetes Mellitus , Health Education , Hypertension , Primary Health Care , Public Health
7.
Biosci. j. (Online) ; 34(2): 423-434, mar./apr. 2018. tab
Article in English | LILACS | ID: biblio-966679

ABSTRACT

The present study aimed measure self-esteem, anxiety and depression and activation scores in individuals with cancer; compare these scores according to whether or not chemotherapy was performed and verify the influence of these constructs in the self-management in health. This study is a Cross-sectional study with a quantitative approach, performed at the outpatient clinic of a school hospital. The instrument was answered by adult patients, diagnosed with cancer for at least 6 months and in outpatient follow-up. We used the Pearson correlation test and the Student t test in order to evaluate the variables of interest. The level of significance was 0.05. Seventy patients participated in the study; forty six of them were women. The average age was 50.17 years (SD = 11.26) and the average of the education level in years was 8.27 (SD = 3.73). The most prevalent cancer was the breast cancer. The average score obtained by the instruments were: 32.71 (SD = 4.64) for self-esteem; 6.07 (SD = 3.84) for anxiety; 4.90 (SD = 3.76) for depression and 64.24 (SD = 15.21) for activation. We observed weak correlations of these constructs with activation (p <0.05) and that the use of chemotherapy did not interfere with the score of these measures (p> 0.05). The education level presented a weak correlation with the measure of activation (r = 0.240, p <0.05), and comorbidities presented weak correlation with the measure of self-esteem (r = -0.274, p <0.05). In conclusion, the cancer patients presented high selfesteem, low anxiety and depression symptoms and high activation. There is a weak relationship between measures evaluated and the self-management in health.


O presente estudo objetivou mensurar os escores de autoestima, ansiedade e depressão e de ativação em indivíduos com câncer, comparar esses escores segundo a realização ou não de quimioterapia e verificar a influência desses constructos na autogestão em saúde. É um estudo transversal com abordagem quantitativa, realizado no ambulatório de um hospital Universitário. Pacientes adultos, com diagnóstico de câncer, há pelo menos 6 meses e em acompanhamento ambulatorial, responderam os Instrumentos. Utilizamos testes de correlação de Pearson e t de student para avaliar as variáveis de interesse. Nível de significância de 0,05. Participaram 70 pacientes, sendo 46 mulheres. A idade média foi 50,17 anos (DP=11,26) e o tempo médio de escolaridade foi 8,27 anos (DP=3,73). O tipo de câncer mais prevalente foi o de mama. Os escores médios obtidos pelos instrumentos foram: 32,71 (DP=4,64) para autoestima, 6,07 (DP=3,84) para ansiedade, 4,90 (DP=3,76) para depressão e de 64,24 (DP=15,21) para ativação. Verificamos correlações fracas desses constructos com ativação (p<0,05) e que a realização ou não de quimioterapia não interferiu com as pontuações dessas medidas (p>0,05). A escolaridade apresentou correlação fraca com a medida de ativação (r=0,240, p<0,05), e o número de comorbidades apresentou correlação de magnitude fraca com a medida de autoestima (r= -0,274, p<0,05). Concluímos que os pacientes com câncer apresentaram autoestima elevada, sintomas de ansiedade e depressão baixos e ativação elevado. Há uma relação fraca entre as medidas avaliadas com a autogestão em saúde.


Subject(s)
Anxiety , Patients , Depression , Neoplasms , Self Care , Self Concept
8.
Pesqui. prát. psicossociais ; 11(3): 570-582, dez. 2016.
Article in Portuguese | LILACS | ID: biblio-955322

ABSTRACT

Este artigo versa sobre as possibilidades de uma formação para atenção aos usuários de drogas que seja consonante aos princípios da Política do Ministério da Saúde para atenção integral de usuários de álcool e outras drogas, no sentido de qualificar as propostas de formação dos profissionais. Trata-se de em estudo exploratório, cujo objetivo é apresentar uma proposta de formação implementada pelo Centro de Referência Regional para formação em álcool e outras drogas da Universidade Federal Fluminense. O artigo aponta eixos estruturantes para uma formação diferenciada, como a questão do estigma e a parceria com os serviços de saúde. Para a produção de atos de cuidado, é necessário dimensionar a importância de processos formativos diferenciados para que novos modos de produção de saúde possam ser adotados.


This study presentssomepossibilities for proposing a professional training on attention to drug users that is in accordancewith the principles of the National Health Ministry Policy to the attentionof drug users, in order to qualify the proposed training models. This is an exploratorystudy aiming to present a proposal ofthe training implemented by the Regional Reference Center (CRR) of Fluminense Federal University (UFF) for medical training in alcohol and other drugs. The article points out structural bases for a different formation such as crossing the stigma issue and the partnership with the health system. For the production of health care activities, we need to measure the importance of different training processes sonew health production methods can be adopted.


Este artículo se ocupa de las posibilidades de formación para la atención a los usuarios de drogas que estéen consonancia con los principios de la política de salud para la atención integral de los usuarios de alcohol y otras drogas, con el fin de mejorarlas propuestas de formación profesional. Se trata de un estudio exploratorio cuyo objetivo es presentar una propuesta para la formación implementadapor el Centro Regional de Referencia para la formación en alcohol y otras drogas de la Universidad Federal Fluminense. El artículo señala líneas de construcción para unaformación diferenciada, como el tema del estigma y la colaboracióncon los servicios de salud. Para la producción de las actividades de cuidado es necesario dimensionar la importancia de los diferentes procesos de formación para que nuevos métodos de producción de la salud puedan ser adoptados.


Subject(s)
Illicit Drugs , Education , Comprehensive Health Care , Professional Training , Health Services
9.
Appl Biochem Biotechnol ; 169(6): 1952-64, 2013 Mar.
Article in English | MEDLINE | ID: mdl-23354500

ABSTRACT

The metabolism of residual glycerol from biodiesel synthesis by Klebsiella pneumoniae BLh-1 was investigated in this study. Batch and fed-batch cultivations were performed in bioreactors under anaerobic and oxygen limitation conditions. Results of batch cultivations showed that the main product was 1,3-propanediol (1,3-PD) in both conditions, although the higher yields and productivities (0.46 mol mol(-1) glycerol and 1.22 g L(-1) h(-1), respectively) were obtained under anaerobic condition. Large amounts of ethanol were also produced under batch anaerobic condition, peaking at 12.30 g L(-1). Batch cultivations under oxygen limitation were characterized by faster growth kinetics, with higher biomass production but lower conversions of glycerol into 1,3-PD, with yields and productivities of 0.33 mol mol(-1) glycerol and 0.99 g L(-1) h(-1), respectively. The fed-batch cultivations were carried out in order to investigate the effects of feeding of raw glycerol on cells. Fed-batch under anaerobiosis showed that 1,3-PD and ethanol concentrations increased with the feeding rate, with maximal productions of 26.12 and 19.2 g L(-1), respectively. The oxygen limitation conditions diverted the bacterium metabolism to an elevated lactic acid formation, reaching 59 g L(-1) in higher feeding rates of glycerol, but lowering the production of ethanol.


Subject(s)
Batch Cell Culture Techniques/methods , Biofuels , Glycerol/metabolism , Klebsiella pneumoniae/growth & development , Klebsiella pneumoniae/metabolism , Oxygen/metabolism , Anaerobiosis , Glycerol/pharmacology , Klebsiella pneumoniae/drug effects
10.
Molecules ; 16(12): 9827-37, 2011 Nov 25.
Article in English | MEDLINE | ID: mdl-22117172

ABSTRACT

The chemical composition and antimicrobial activity of essential oils obtained from leaves of two Myrtaceae species-Eugenia uniflora L. and Plinia trunciflora (O. Berg) Kausel-were determined. Analysis by GC/MS as well as determination of Kovatz indexes indicated atractylone (26.78%) and curzerene (17.96%) as major constituents of E. uniflora oil and α-cadinol (19.15%), apiole (11.15%) and cubenol (5.43%) as main components in P. trunciflora oil. Both essential oils were tested for antimicrobial activity against yeasts and bacteria. E. uniflora and P. trunciflora essential oils were active towards two Gram-positive bacteria, Streptococcus equi and Staphylococcus epidermis. In addition, biological activity of both essential oils was detected for pathogenic yeasts of the genus Candida and Cryptococcus. E. uniflora was active towards all yeast tested and exhibited interesting minimal inhibitory concentrations (0.11 to 3.75 mg/mL) across a broad spectrum of activity.


Subject(s)
Myrtaceae/chemistry , Oils, Volatile/chemistry , Oils, Volatile/pharmacology , Syzygium/chemistry , Anti-Bacterial Agents/pharmacology , Bacteria/drug effects , Fungi/drug effects , Microbial Sensitivity Tests , Plant Leaves/chemistry , Species Specificity
11.
Article in English | MEDLINE | ID: mdl-21967246

ABSTRACT

In this research the production of hydrogen by Klebsiella pneumoniae BLb01 using residual glycerol discharged from a biodiesel fuel production plant was investigated. Klebsiella pneumoniae BLb01 was isolated from a bacteria-rich sludge of an upflow anaerobic sludge blanket reactor (UASB) of a soybean processing plant. A Plackett-Burman design (P-B) and Response Surface Methodology (RSM) were employed to determine the optimal condition for enhanced hydrogen production. The maximal hydrogen production, which was 45.0 mol % and with 98% of glycerol degradation, was achieved with the optimized medium with the following composition: 30 g L(-1) glycerol; 3 g L(-1) yeast ex tract 3 g L(-1) K(2)HPO(4); 1 g L(-1) KH(2)PO(4); temperature 39°C and pH 9.0. These results show the ability of this new strain of effectively converting residual glycerol into value-added energy products.


Subject(s)
Bioelectric Energy Sources , Biofuels/analysis , Bioreactors , Glycerol/metabolism , Hydrogen/metabolism , Klebsiella pneumoniae/metabolism , Glycerol/chemistry , Hydrogen-Ion Concentration , Temperature
12.
Enzyme Res ; 2011: 679624, 2011.
Article in English | MEDLINE | ID: mdl-21845217

ABSTRACT

Composting is a way of transforming the organic waste into fertilizer, minimizing the use of inorganic compounds that may contaminate the environment. This transformation is the result of the microorganism action, converting complex carbon sources into energy. Enzymes that are exported by the microorganisms to the surrounding environment mediate this process. The aiming of the present work is to prospect the compost produced by the organic composting unit (OCU) of the Fundação Parque Zoológico de São Paulo (FPZSP) to find novel starch hydrolyzing organisms (SHO) that secrete large amounts of amylases under harsh conditions, such as high temperature. We found five bacterial isolates that have amylolytic activity induced by soluble starch and 39°C temperature of growth. These bacterial strains were identified by MALDI-TOF (Matrix-assisted laser desorption/ionization-Time of Flight) analysis, a rapid and efficient methodology for microbe identification in large scale. Our results present amylolytic strains that belong to diverse taxonomic groups (Solibacillus silvestris, Arthrobacter arilaitensis, Isoptericola variabilis, and Acinetobacter calcoaceticus); some of them have never been associated with this kind of hydrolytic activity before. The information regarding enzyme induction will be important to optimize the production by the bacterial isolates, which may be a great value for biotechnological applications.

13.
Acta sci., Biol. sci ; 31(2): 195-201, 2009.
Article in Portuguese | LILACS-Express | LILACS, VETINDEX | ID: biblio-1460582

ABSTRACT

This paper verified possible alterations caused by a genotype II Toxoplasma gondii strain with respect to the total number and morphometry of the myenteric neurons in the terminal ileum and descending colon of rats. Eight rats were divided into two groups: control (n = 4) and experimental (n = 4). This group was inoculated orally with 20 tissue cysts of T. gondii from a genotype II strain (ME-49). Whole mounted from the terminal ileum and the descending colon were stained with Giemsa. There was not any neuronal loss on both organs. The neurons became hypertrophied in the terminal ileum, whereas morphometric alterations were not observed for the neurons in the descending colon.


This paper verified possible alterations caused by a genotype II Toxoplasma gondii strain with respect to the total number and morphometry of the myenteric neurons in the terminal ileum and descending colon of rats. Eight rats were divided into two groups: control (n = 4) and experimental (n = 4). This group was inoculated orally with 20 tissue cysts of T. gondii from a genotype II strain (ME-49). Whole mounted from the terminal ileum and the descending colon were stained with Giemsa. There was not any neuronal loss on both organs. The neurons became hypertrophied in the terminal ileum, whereas morphometric alterations were not observed for the neurons in the descending colon.

14.
Arq. ciênc. vet. zool. UNIPAR ; 11(1): 5-10, Jan-Jul. 2008. tab, graf
Article in Portuguese | LILACS | ID: lil-523318

ABSTRACT

Objetivou-se avaliar os efeitos da desnutrição protéica sobre os aspectos morfológicos e quantitativos do plexo mientérico do colón ascendente de ratos adultos. Os animais controle (n=5) receberam ração comercial com 26% de proteína e os animais experimentais (n=4) ração com teor protéico reduzido para 4%, durante 90 dias. Por dissecção, elaboramos preparados totais, que foram corados pela técnica de Giemsa. Os ratos desnutridos apresentaram peso corporal 43,14% menor. A média da área do colón ascendente e a densidade neuronal estavam, respectivamente, 38,90% menor e 25,79% maior, em relação aos animais do grupo controle. Não foi observada redução no número total de neurônios mientéricos do cólon nos animais desnutridos. Dados morfométricos demonstraram aumento na área do pericário destes neurônios.


The effects of protein malnutrition on morphological and quantitative aspects of the myenteric plexus of adult rat ascending colon. The control animals (n=5) received 26%-protein commercial feed while the experimental (n=4) received 4%-protein commercial feed for 90 days. Whole mount preparations were Giemsa stained for dissection. Malnourished rats presented 43.14% lighter body weight. The means of the ascending colon area and the neuronal density were 38.90% lower and 25.79% higher, respectively, in relation to the Control Group. No reduction of the total number of colon myenteric neurons of malnourished animals was noticed. Morphometric data showed an increase of the perikarium of these neurons.


Esta investigación tuvo como objeto evaluar los efectos de la desnutrición proteica sobre los aspectos morfológicos y cuantitativos del plexo mientérico del colon ascendiente de ratones adultos. Los animales control (n=5) recibieron ración comercial con 26% de proteína y los animales experimentales (n=4) ración con tenor proteico reducido para 4%, durante 90 días. Por disección, elaboramos preparados totales, que fueron coloreados por la técnica de Giemsa. Los ratones desnutridos presentaron peso corporal 43,14% menor. La media del área del colon ascendiente y la densidad neuronal estaban, respectivamente, 38,90% menor y 25,79% mayor, en relación a los animales del grupo control. No fue observada reducción en el número total de neuronas del plexo mientérico del colon, en los animales desnutridos. Datos morfométricos demostraron aumento en el área del pericario de estas neuronas.


Subject(s)
Animals , Colon, Ascending , Protein Deficiency , Dissection , Neurons , Myenteric Plexus/anatomy & histology , Rats
15.
Psicol. hosp. (São Paulo) ; 4(2): 1-39, ago. 2006. ilus, tab
Article in Portuguese | Index Psychology - journals | ID: psi-57577

ABSTRACT

O presente estudo teve como objetivo principal investigar, a partir do referencial psicanalítico, as representações de qualidade de vida entre idosos atendidos no Serviço de Geriatria do ICHC-FMUSP (Ambulatório, Enfermaria, Gamia). Foram utilizados: Entrevista semi-dirigida e Procedimento de Desenho-Estória com Tema, conforme técnica proposta por Vaisberg (1997). Os dados foram analisados quantitativamente por meio da Análise de Freqüência de Conteúdos e qualitativamente por meio do referencial psicanalítico. Na enfermaria constatou-se que a vivência da internação interfere significativamente na representação de qualidade de vida. No Gamia, a exigência por uma vida com qualidade é maior por serem idosos mais críticos. No ambulatório, a representação de qualidade de vida está relacionada a características de personalidade e não à vivência ambulatorial. Embora tal estudo tenha sido realizado no âmbito hospitalar quase não apareceram questões clínicas, mas sim questões sociais e emocionais caracterizando a necessidade de um trabalho interdisciplinar.(AU)


The objective of the study was to investigate the representations about quality of life among elders in the Geriatric Service of the ICHC-FMUSP. An Semi-structured and a story drawing procedure as proposed by Vaisberg (1997) were used. The data was analyzed by the Frequency Analysis of Content, and qualitatively by psychological reference. With the in-pacients, it was that the experience of the hospitalization has a significant impact on their perception about quality of life. At the Multidisciplinary Outpatient Care for the Elderly (Acronym in Portuguese: Gamia), the demand for quality of life is greater because the elderly are more critical. At the ambulatory, the representations of quality of life is related to personality characteristics and not to the outpatient experience. Although such study have been done in hospitals, not many clinical issues showed up, but rather social and emotional issues showing the necessity of interdisciplinary work.(AU)

16.
REME rev. min. enferm ; 7(2): 82-88, jul.-dez. 2003.
Article in Portuguese | LILACS, BDENF - Nursing | ID: lil-479295

ABSTRACT

Trata-se de um estudo qualitativo, na vertente fenomenológica, que visa compreender a experiência da paternidade vivenciada pelo adolescente. Foi realizado na Fundação de Assistência Integral à Saúde - Hospital Sofia Feldman. Os sujeitos foram adolescentes, companheiros das gestantes atendidas no pré-natal. Os dados foram coletados através de entrevista aberta, utilizando-se de uma questão norteadora. A partir dos aspectos essenciais emergidos dos depoimentos foram construídas cinco unidades temáticas: A espera do filho como uma experiência agradável; amadurecendo com a paternidade; assumindo a paternidade; a família como suporte necessário; ambivalência de sentimentos. Os resultados obtidos contribuem para direcionar a assistência ao pai adolescente


This is a qualitative phenomenological study which examines the experience of being a father as lived by teenagers. It was carried out at the Comprehensive Health Assistance Foundation of the Sofia Feldman Hospital. The subjects were teenagers aged between 16 and 20, partners of pregnant girls who went for their antenatal appointments. The data was collected through an open interview, guided by a questionnaire. We report five themes arising from the interviews: expecting the baby was a pleasant experience; becoming more mature through pregnancy; becoming more responsible; taking on the role of father; family as a necessary support; ambivalent feelings. The results of this work help guide assistance to teenage fathers


Se trata de un estudio cualitativo con enfoque fenomenológico que busca comprender la experiencia de la paternidad del adolescente. Fue realizado en la Fundación de Asistencia Integral a la Salud - Hospital Sofia Feldman. Los sujetos eran adolescentes, compañeros de las embarazadas atendidas en el prenatal. Los datos se colectaron en entrevista abierta, con un cuestionario que sirvió de guía. Se identificaron los aspectos esenciales de las declaraciones y se elaboraron cinco unidades temáticas: la espera del hijo como una experiencia agradable; madurando con la paternidad; asumiendo la paternidad; la familia como soporte necesario; ambivalencia de sentimientos. Los resultados obtenidos contribuyen a orientar la asistencia al padre adolescente


Subject(s)
Humans , Female , Pregnancy , Pregnancy in Adolescence , Paternity , Interviews as Topic , Existentialism
17.
Arq. biol. tecnol ; 39(4): 831-9, dez.1996. tab, graf
Article in Portuguese | LILACS | ID: lil-238883

ABSTRACT

A strain of Rhizopus sp. screened among more than 800 filamentous fungi showed great ability to produce a thermostable alfa-amylase by solid state fermentation. The best production was obtained with a bran moisture content of 40 per cent when the enzyme activity reached 60 EU/g. of medium. During the purification procedures, a column of DEAE- Sephadex A-50 separeted the enzyme in two fractions and the larger (85 per cent of the total activity) showed optimum pH in a range from 4.0 to 5.6. Optimum temperature was found at 60-65§ C and in this range no loss of activity was observed after 60 min. of treatment in pH 5,0. Its Km and Vm are, respectively, of 5.0 mg/ml of starch and 10,01 uMol of reducing sugar/min./mg. of protein. Its molecular weight was calculated in 64.000 by gel filtration in Sephadex G-200. The dextrinization power of the enzyme was observed preferentialy on substrates compound by chain with higher ramifications, traht is: amylopectin > starch> amylose. Other aspects of the enzyme pattern action are also discussed


Subject(s)
alpha-Amylases , Amylopectin , Amylose , Enzyme Induction , Food Technology , Rhizopus , Starch
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