Factors that influence trace element levels in blood and feathers of Pygoscelis penguins from South Shetland Islands, Antarctica.

Contaminant levels are lower in Antarctica than elsewhere in the world because of its low anthropogenic activities. However, the northern region of the Antarctic Peninsula, is close to South America and experiences the greatest anthropogenic pressure in Antarctica. Here, we investigated, in two Antarctic Peninsula islands, intra and interspecific factors that influence the concentrations of 17 trace elements (TEs) in blood and feathers of three penguin species breeding sympatrically in relation to their trophic ecology assessed via a stable isotopic approach (C, N and S). Geographical location, foraging zone (δ13C and δ34S) and diet influences the interspecific difference, and sex and maturity stage diet influence the intraspecific difference of Pygoscelis penguins. Penguins from Livingston showed higher values (mean, ng. g-1, dry weight - dw) of Zn (103), Mn (0.3), and Fe (95) than those from King George Island (Zn: 80, Mn: 1.9, and Fe: 11). Gender-related differences were observed, as males showed significantly higher values (mean, ng. g-1, dw) of Rb (3.4) and δ15N in blood of gentoo, and Ca (1344) in Adélie feathers. Chicks of gentoo and Adélie presented higher Zn, Mg, Ca, and Sr and lower 13C values in blood than adults. The highest concentrations (mean, ng. g-1, dw) of Cd (0.2) and Cu (26), and the lowest δ15N values were found in chinstrap. Geographical, intraspecific (i.e., ontogenetic and gender-related) and interspecific differences in feeding seemed to have influenced TE and stable isotope values in these animals. The TE bioaccumulation by penguins may have also been influenced by natural enrichment in environmental levels of these elements, which seems to be the case for Fe, Zn, and Mn. However, the high level of some of the TEs (Mn, Cd, and Cr) may reflect the increase of local and global human activities.

Biogenesis of circular RNAs and their role in cellular and molecular phenotypes of neurological disorders.

Circular RNA (circRNA) is an unusual class of RNA-like structures composed by exonic and/or intronic sequences that are regulated by the backsplicing mechanism and by the spliceosome-mediated machinery. These circular transcripts tend to accumulate during aging in several human tissues, especially in the mammalian brain, and their expression is correlated with the occurrence of several human pathologies, including a broad spectrum of neurological disorders. Previous findings have also shown that circRNAs are significantly present in the neuronal tissue and are up-regulated during neurogenesis, with a significant number been derived from neural genes, suggesting these circular molecules are involved in the cellular and molecular phenotype of our brain. However, the complete biogenesis, the many types of circRNA molecules, and their involvement with neuronal phenotype and with the occurrence of pathologies are still a challenging avenue for researchers. In this updated review, we discuss the current findings of the biogenesis and the diversity of cirRNAs and their molecular involvement in neurological tissue phenotype. We also discuss how some circRNAs can act as sponge molecules, regulating the activity of microRNA expression over gene translation. Finally, we also show the correlation of altered circRNA expression in neurological disorders.

The evolution of ADAM gene family in eukaryotes.

The ADAM (A Disintegrin And Metalloprotease) gene family encodes proteins with adhesion and proteolytic functions. ADAM proteins are associated with diseases like cancers. Twenty ADAM genes have been identified in humans. However, little is known about the evolution of the family. We analyzed the repertoire of ADAM genes in a vast number of eukaryotic genomes to clarify the main gene copy number expansions. For the first time, we provide compelling evidence that early-branching green algae (Mamiellophyceae) have ADAM genes, suggesting that they originated in the last common ancestor of eukaryotes, before the split of plants, fungi and animals. The ADAM family expanded in early metazoans, with the most significative gene expansion happening during the first steps of vertebrate evolution. We concluded that most of mammal ADAM diversity can be explained by gene duplications in early bone fish. Our data suggest that ADAM genes were lost early in green plant evolution.

Forage structure and performance of sheep raised on massai grass during the dry season in response to wet season management / Estrutura do pasto e desempenho de ovinos em capim-massai na época seca em resposta ao manejo do período das águas

The objective of this study was to evaluate the canopy structure and performance of sheep raised on massai grass during the dry season in response to the management adopted during the wet season. The treatments consisted of two targets of light interception (90 and 95%) and two post-grazing heights (15 and 25 cm) in a 2 x 2 factorial scheme. Pastures were managed during the wet season, while continuous stocking was used during the dry season. The following pasture characteristics were evaluated: canopy height, forage mass, leaf blade, stem and dead matter mass, leaf blade:stem ratio, crude protein, neutral detergent fiber, acid detergent fiber and acid detergent lignin content, and dead matter of morphological components. Sixteen sheep with an initial body weight of 19 ± 4.7 kg were used and average daily gain, weight gain per hectare and stocking rate were evaluated. The interactions between sources of variation were not significant for any of the variables. Post-grazing heights modified total forage and dead matter mass in the dry season, with the highest values of 2912.5 and 1840.9 kg/ha of dry matter, respectively, being observed when the pastures were managed at 25 cm during the wet season. There was no effect of the management adopted during the wet season on the chemical composition of leaf blades during the dry season...(AU)

O objetivo do trabalho foi avaliar a estrutura do dossel e o desempenho de ovinos durante a estação seca em resposta ao manejo adotado nas águas em pastos de capim-massai. Os tratamentos consistiram do arranjo fatorial 2 x 2, duas metas de interceptação de luz (90% e 95%) e duas alturas de pós-pastejo (15 e 25 cm). Os manejos foram realizados no período chuvoso enquanto que no período seco o pasto foi manejado sob lotação contínua. No pasto foi avaliada a altura do dossel, massa de forragem, massa de lâmina foliar, massa de colmo, massa de material morto, relação lâmina foliar:colmo, os teores de proteína bruta, fibra em detergente neutro, fibra em detergente ácido, lignina em detergente ácido dos componentes morfológicos. Foram utilizados 16 ovinos com peso corporal inicial de 19 ± 4,7 kg, e avaliado o ganho de peso médio diário, o ganho de peso por hectare e a taxa de lotação. As interações entre as fontes de variações não foram significativas para nenhuma das variáveis. As alturas de pós-pastejo modificaram as massas de forragem total e de material morto na época de seca, quando manejados nas águas a 25 cm foram observados os maiores valores de 2912,5 e 1840,9 kg/ha de matéria seca, respectivamente. Não houve efeito dos manejos adotados no período das águas na composição química das lâminas foliares no período seco...(AU)

Forage structure and performance of sheep raised on massai grass during the dry season in response to wet season management / Estrutura do pasto e desempenho de ovinos em capim-massai na época seca em resposta ao manejo do período das águas

The objective of this study was to evaluate the canopy structure and performance of sheep raised on massai grass during the dry season in response to the management adopted during the wet season. The treatments consisted of two targets of light interception (90 and 95%) and two post-grazing heights (15 and 25 cm) in a 2 x 2 factorial scheme. Pastures were managed during the wet season, while continuous stocking was used during the dry season. The following pasture characteristics were evaluated: canopy height, forage mass, leaf blade, stem and dead matter mass, leaf blade:stem ratio, crude protein, neutral detergent fiber, acid detergent fiber and acid detergent lignin content, and dead matter of morphological components. Sixteen sheep with an initial body weight of 19 ± 4.7 kg were used and average daily gain, weight gain per hectare and stocking rate were evaluated. The interactions between sources of variation were not significant for any of the variables. Post-grazing heights modified total forage and dead matter mass in the dry season, with the highest values of 2912.5 and 1840.9 kg/ha of dry matter, respectively, being observed when the pastures were managed at 25 cm during the wet season. There was no effect of the management adopted during the wet season on the chemical composition of leaf blades during the dry season...

O objetivo do trabalho foi avaliar a estrutura do dossel e o desempenho de ovinos durante a estação seca em resposta ao manejo adotado nas águas em pastos de capim-massai. Os tratamentos consistiram do arranjo fatorial 2 x 2, duas metas de interceptação de luz (90% e 95%) e duas alturas de pós-pastejo (15 e 25 cm). Os manejos foram realizados no período chuvoso enquanto que no período seco o pasto foi manejado sob lotação contínua. No pasto foi avaliada a altura do dossel, massa de forragem, massa de lâmina foliar, massa de colmo, massa de material morto, relação lâmina foliar:colmo, os teores de proteína bruta, fibra em detergente neutro, fibra em detergente ácido, lignina em detergente ácido dos componentes morfológicos. Foram utilizados 16 ovinos com peso corporal inicial de 19 ± 4,7 kg, e avaliado o ganho de peso médio diário, o ganho de peso por hectare e a taxa de lotação. As interações entre as fontes de variações não foram significativas para nenhuma das variáveis. As alturas de pós-pastejo modificaram as massas de forragem total e de material morto na época de seca, quando manejados nas águas a 25 cm foram observados os maiores valores de 2912,5 e 1840,9 kg/ha de matéria seca, respectivamente. Não houve efeito dos manejos adotados no período das águas na composição química das lâminas foliares no período seco...

Germinação de sementes de Aspidosperma subincanumMart. ex A. DC em diferentes temperaturas / Germination of Aspidosperma subincanum Mart. ex A. DC seeds at different temperatures

RESUMO : Aspidosperma subincanum é uma espécie arbórea utilizada em projetos de recuperação de áreas degradadas e como planta medicinal. Levando-se em consideração a importância da espécie, o presente trabalho teve como objetivo definir a temperatura ideal para a germinação de suas sementes. O material vegetal foi coletado na região do Pantanal de Miranda, Mato Grosso do Sul. As sementes foram colocadas em caixas plásticas transparentes em câmara de germinação e submetidas às temperaturas constantes de 20, 25, 30 e 35°C e alternadas de 20-30 e 25-35°C. O delineamento experimental foi inteiramente casualizado, com quatro repetições por tratamento, sendo as médias comparadas pelo teste de Tukey a 5%. A temperatura ideal para a germinação de Aspidosperma subincanum foi a 25°C, com o maior índice de velocidade de germinação, 18,8. Temperaturas mais elevadas (30 e 35°C) causaram a morte dos embriões

ABSTRACT Germination of Aspidosperma subincanum Mart. ex A. DC seeds at different temperatures. The Aspidosperma subincanumem is a tree species used in the recovery of degraded areas projects and as a medicinal plant. Taking into account the importance of the species, the current study aimed to determine which would be the ideal temperature for the germination of their seeds. The plant material was collected in the Pantanal of Miranda, Mato Grosso do Sul. The seeds were placed in transparent boxes in germination chambers and submitted to constant temperatures of, 20, 25, 30 and 35°C and alternated at 20-30 and 25- 35°C, with four replicates per treatment. The statistical design was a completely randomized one. The averages were compared by Tukey test at 5%. The ideal temperature for germination of Aspidosperma subincanum was 25°C. The high temperatures (30 and 35°C) have caused the death of embryos

First Report of Anthracnose on Pepper Fruit Caused by Colletotrichum scovillei in Brazil.

Anthracnose is major disease of pepper (Capsicum annum) in the tropics and causes severe damage both in the field and postharvest. In Brazil, this disease is caused by Colletotrichum acutatum, C. boninense, C. capsici, C. coccodes, and C. gloeosporioides, where the first species is responsible for 70% of all occurrences (3). Recently, C. acutatum has been considered a species complex (1); thus, the aim of this study was to verify the etiology of anthracnose on peppers using a morphological and molecular approaches. In 2011, pepper fruits with typical symptoms of anthracnose (dark, sunken spots with concentric rings of orange conidial masses) were collected in Viçosa, Minas Gerais, Brazil. A single spore isolate was obtained on potato dextrose agar (PDA), and the derived culture was deposited in the Coleção de Culturas de Fungos Fitopatogênicos "Prof. Maria Menezes" (code CMM-4200). The upper side colonies on PDA were gray, cotton-like, and pale gray to pale orange. Conidia were hyaline, aseptate, smooth, straight, cylindrical with round ends or occasionally with end ± acute, 12.5 to 17 µm long and 3.5 to 4 µm wide on synthetic nutrient deficient agar. The isolate was morphologically typical of species belonging to the C. acutatum complex. Molecular identification of the pathogen was carried out and sequences of the regions internal transcribed spacer (ITS), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and ß-tubulin (ßt) were obtained and deposited in GenBank (Accession Nos. KJ541821 to KJ541823). A search in the Q-bank fungi database using the ITS, ßt, and GAPDH sequences retrieved C. scovillei with 100% identity for all three genes. This pathogen was previously reported in Capsicum spp. only in Thailand, Indonesia, and Japan (1,2). To confirm pathogenicity, drops with 105 spores/ml were deposited in 10 artificially wounded fruits (cv. Itapuã 501 and Melina). In control fruits, drops of sterilized water were deposited onto wounds. The fruits were covered for one day with a transparent plastic bag with moisture supplied by a wet filter paper. The fruits were detached and mature. The bags were removed, and the fruits were incubated for 10 days in a growth chamber at 25°C with a photoperiod of 12 h. After 4 days, gray-brown to black sunken spots with concentric rings were observed on 100% of the wounded fruits that had been inoculated. No disease was observed on the control fruits. The fungus C. scovillei was successfully re-isolated from symptomatic fruits to fulfill Koch's postulates. To our knowledge, this is the first report of anthracnose on pepper fruit caused by C. scovillei in Brazil. Due to the diversity of species that cause anthracnose in Capsicum, future studies using morphological and molecular tools are essential for the correct identification of Colletotrichum spp. on pepper in Brazil. References: (1) U. Damm et al. Stud. Mycol. 73:37, 2012. (2) T. Kanto et al. J. Gen. Plant. Pathol. 80:73, 2014. (3) M. J. Z. Pereira et al. Hortic. Bras. 29:569, 2011.

Análise de medicamentos antiinflamatórios não esteroidais manipulados empregando espectroscopia de reflexão difusa no infravermelho com transformada de Fourier (DRIFTS) / Analysis of nonsteroidal antiinflammatory drugs in compounded medicines by diffuse-reflectance infrared Fourier transform spectroscopy (DRIFTS)

A análise de dois fármacos diferentes, mas estruturalmente semelhantes, foi realizada, empregando espectros de reflexão difusa no infravermelho médio com transformada de Fourier (DRIFTS), em associação com a técnica de análise por agrupamentos hierárquicos (AAH). Amostras de 3 diferentes farmácias de manipulação, contendo diclofenaco de sódio 50mg, diclofenaco de potássio 50mg e seus respectivos excipientes, foram analisadas em duplicata por dois analistas. Para a análise multivariada foi empregado o aplicativo Pirouette R 2.7 da Infometrix, selecionando-se o conjunto de regiões dos espectros com maior número de informações. Os dendogramas foram construídos com os dados auto-escalados e correção do espalhamento de luz (MSC), utilizando três tipos de construções: simples, flexível e incremental. Com a aplicação da AAH, constatou-se a formação de diferentes grupos obedecendo à discriminação de cada princípio ativo, indicando seus fornecedores e, em separado, outro grupo. Estes resultados demonstram que a técnica DRIFTS, em conjunto com ferramentas quimioterápicas, constitui uma excelente opção para a caracterização de fármacos, gerando uma inovadora metodologia para auxiliar no controle da qualidade de processos industriais de produção de medicamentos

Pneumotórax espontâneo e câncer de mamam / Spontaneous pneumothorax and breast cancer

Pneumotórax espontâneo (PE) concomitante às doenças neoplásicas é incomum. Destas, as que mais se associam ao PE säo o câncer de pulmäo e o sarcoma osteogênico com metástases pulmonares. Objetivo. O presente artigo tem o intuito de registrar a ocorrência de PE relacionado a neoplasia de mama e discutir os principais fatores etiopatogênicos envolvidos no evento. Material e Métodos. Os autores descrevem caso de mulher com 43 anos, parda, näo tabagista e protadora de carcinoma ductal infiltrante de mama esquerda, com metástases pulmonares disseminadas bilateralmente. O PE ocorreu na vigência de poliquimioterapia e näo pode ser relacionado a outras causas. Conclusäo. Embora o pulmäo apresente metástases conseqüentes a neoplasia mamária em até 60 por cento dos casos, a coexistência de PE e carcinoma de mama é muito rara. Em revisäo de literatura dos últimos cinco anos, näo se encontraram relatos desta concomitância. Quanto à etiopatogenia do pneumotórax, acredita-se que os fatores relacionados ao episódio sejam múltimos, e os autores fazem comentários a respeito

[Spontaneous pneumothorax and breast cancer]. / Pneumotórax espontâneo e câncer de mama.

BACKGROUND: Spontaneous pneumothorax with concomitant neoplastic diseases is unusual. Lung cancer and osteogenic sarcoma with pulmonary metastasis are the neoplasms that most frequently accompany the spontaneous pneumothorax. This article's purposes are to relate the occurrence of spontaneous pneumothorax associated with breast neoplasms and to discuss the main etiopathogenic factors involved in the process. MATERIAL AND METHODS: The authors report a case of a 43-year-old black woman carrier of a left breast ductal carcinoma with bilateral pulmonary metastasis. Spontaneous pneumothorax appeared during multidrug chemotherapy, so it cannot be related to other causes. CONCLUSIONS: Even though breast neoplasms cause lung metastases in about 60% of the cases, the coexistence of spontaneous pneumothorax and breast carcinoma is very rare. There was no report of this topic in the medical literature review during the last five years. The authors believe and discuss the multiple pneumothorax etiopathogenic factors related to this case.

Fratura do penis durante intercurso sexual: relato de um caso. / Fracture of the penis during sexual intercourse: report of a case

Os autores apresentam um caso de fratura de penis em paciente jovem durante o intercurso sexual. Comentam sobre a etiologia, quadro clinico e o tratamento, ressaltando a importancia da cirurgia imediata