A randomized clinical trial evaluating maxillary sinus augmentation with different particle sizes of demineralized bovine bone mineral: histological and immunohistochemical analysis.

This study was performed to investigate sinus floor augmentation with two different particle sizes of demineralized bovine bone mineral (DBBM) by means of histological and immunohistochemical (IHC) analysis. A randomized clinical trial was conducted involving 10 individuals requiring two-stage bilateral maxillary sinus augmentation for implant installation. The patients were randomly divided into two groups following a split-mouth design: the maxillary sinus on one side was filled with small-sized particles (0.25-1mm) and on the contralateral side with large-sized particles (1-2mm). After a healing period of 8 months, 25 implants were placed. During implant site preparation, bone biopsies were obtained from each sinus, perpendicular to the long axis of the implant (buccal-palatal direction), for descriptive and histomorphometric analyses. IHC staining for protein expression of osteocalcin (OCN), vascular endothelial growth factor (VEGF), and tartrate-resistant acid phosphatase (TRAP) was also performed. Histomorphometric analysis revealed no statistically significant difference in the percentage of biomaterial (32.4±8.56% and 38.0±6.92%), newly formed bone (36.1±9.60% and 36.7±5.79%), or connective tissue (30.4±8.63% and 23.8±6.16%) between the small- and large-sized particle groups, respectively. IHC analysis did not reveal differences in the expression of OCN, VEGF, or TRAP. These findings suggest that both particle sizes of DBBM are effective for bone augmentation in the maxillary sinus.

O-naphthoquinone isolated from Capraria biflora L. induces selective cytotoxicity in tumor cell lines.

Biflorin is an o-naphthoquinone isolated from the roots of the plant Capraria biflora L. (Scrophulariaceae). In this study, the cytotoxic effects of biflorin were verified, and late apoptosis was detected in various cancer cell lines by in situ analysis. The cytotoxicity was further evaluated exclusively for 48 h of treatment in different tumor and non-tumor cell lines (Hep-2, HeLa, HT-29, A-375, and A-549, and HEK-293, respectively). The results indicated that biflorin induced selective cytotoxicity in tumor cells. HeLa cells were more susceptible to biflorin, followed by HT-29, A-549, A-375, and Hep-2 at all concentrations (range 5-50 µg/mL), and the highest half-maximal inhibitory concentration IC50 (56.01 ± 1.17 µg/mL) was observed in HEK-293 cells. Late apoptotic/necrotic events, observed by in situ immunostaining with Annexin V, varied with each cell line; an increase in late apoptotic events was observed corresponding to the increase in biflorin dosage. Hep-2 cells showed a greater percentage of late apoptotic events among the tumor cell lines when treated with higher concentrations of biflorin (69.63 ± 2.28%). The non-tumor HEK-293 line showed greater resistance to late apoptotic events, as well as a lower level of cytotoxicity (77.69 ± 6.68%) than the tested tumor lines. The data presented indicate that biflorin showed an important, possibly selective, cytotoxicity against tumor cell lines, thereby revealing a promising novel substance with potential anticancer activity for tumor therapy.

Development of topotecan loaded lipid nanoparticles for chemical stabilization and prolonged release.

Topotecan is an important cytotoxic drug that has gained broad acceptance in clinical use for the treatment of refractory ovarian and small-cell lung cancer. The lactone active form of topotecan can be hydrolyzed in vivo, decreasing the drug's therapeutic efficacy. Lipid encapsulation may promote in vivo stabilization by removing topotecan from aqueous media. Earlier reports of topotecan lipid nanoencapsulation have focused on liposomal encapsulation; however, the higher stability and cost-effectiveness of solid lipid nanoparticles (SLN) highlight the potential of these nanoparticles as an advantageous carrier for topotecan. The initial motivation for this work was to develop, for the first time, solid lipid nanoparticles and nanostructured lipid carriers (NLC) with a high drug loading for topotecan. A microemulsion technique was employed to prepare SLNs and NLCs and produced homogeneous, small size, negatively charged lipid nanoparticles with high entrapment efficiency and satisfactory drug loading. However, low recovery of topotecan was observed when the microemulsion temperature was high and in order to obtain high quality nanoparticles, and precise control of the microemulsion temperature is critical. Nanoencapsulation sustained topotecan release and improved its chemical stability and cytotoxicity. Surprisingly, there were no significant differences between the NLCs and SLNs, and both are potential carriers for topotecan delivery.

Ticks (Acari: Ixodidae) on dogs from Uberlândia, Minas Gerais, Brazil.

Uberlândia in Minas Gerais State, southeastern Brazil, has 622 000 inhabitants and is located in the Cerrado biome, the South American savannah. The city dog population is estimated at 82 000 and identification of tick species and infestation prevalence on this host has not been determined. A major infectious disease of dogs in the city, canine ehrlichiosis, is transmitted by Rhipicephalus sanguineus ticks. At the same time, autochthonous leishmaniosis has been recently described in the city and a role for dog ticks in the disease transmission has been supposed in Brazil. In this work, we present general information on dog ticks in Uberlândia municipality and region. Dogs from 33 farms and 31 districts were examined for ticks from July 2007 to February 2009. On the whole, 413 dogs were examined, 311 (75.3%) from the city and 102 (24.6%) from rural area. Overall infestation rate of dogs from Uberlândia was 37.3% and the mean infestation intensity was 3.25 parasites per dog. In the urban area, 100 dogs (32.2%) had ticks whereas 54 dogs (52.9%) from rural areas were infested. Four tick species were found: Rhipicephalus sanguineus, Amblyomma cajennense, Amblyomma ovale and Boophilus microplus. In the city, only R. sanguineus and one A. cajennense was found on dogs and R. sanguineus and A. ovale were the main dog ticks in rural areas.

Comparação de metodologias de seleção sob oscilação genética / Comparison of selection methodologies under genetic drift

Determinou-se o número adequado de repetições na comparação de métodos de seleção tradicionais e associados a marcadores moleculares, com diferentes tamanhos efetivos e sob diferentes sistemas de acasalamento dos reprodutores selecionados, usando simulação com o programa GENESYS. Para comparar os diferentes métodos de seleção utilizaram-se populações com tamanhos efetivos de 18,18 (TE1) e de 66,66 (TE2) e uma, 10 e 30 repetições por geração, avaliando-se os valores fenotípicos médios. Para as situações com apenas uma repetição, os resultados apresentaram incoerências, independentemente do tamanho efetivo (TE1 ou TE2) ou do sistema de acasalamento (RAA - reprodutores acasalados aleatoriamente, EIC - exclusão de irmãos completos ou EICMI - exclusão de irmãos completos e meio-irmãos). Observou-se que a oscilação genética influencia o ganho genético, principalmente, em populações com pequeno tamanho efetivo e que um valor mínimo de 10 repetições por geração é necessário para assegurar a consistência dos resultados obtidos pelos métodos de seleção.

This work was carried out to determine the required number of replicates for comparison of conventional and molecular marker-associated selection methods in distinct effective population sizes and different mating systems, by simulations using the software GENESYS. Effective populational size of 18.18 (ES1) and 66.66 (ES2), and one, 10, and 30 replicates per generation were used to compare the different selection methods, based on mean phenotypic values. Incongruences results were observed when a single replicate was considered independently of effective size (ES1or ES2) and the mating system (random mating; exclusion of full-sibs or exclusion of both full and half-sibs). Genetic oscillation influenced the genetic gain, mainly in populations of small effective size. Furthermore, at least 10 replicates per generation were required to obtain sound consistent results for both selection methods.

The 22-mm vs the 32-mm femoral head in cemented primary hip arthroplasty long-term clinical and radiological follow-up study.

A retrospective clinical and radiological analysis was performed on 117 patients (138 hips) with a mean age of 75 years and a mean follow-up of 11 years. The prostheses used were 2 similar monoblock femoral components with 22- and 32-mm head sizes; their effects on the wear of the high-density polyethylene cup were studied. Wear of component was determined by evaluating the standardized initial and follow-up radiographs. The mean rate of linear wear was higher in the 22-mm femoral head, whereas the volumetric wear was higher in the 32-mm femoral head. In both groups, volumetric wear was significantly correlated with cup lysis. Increasing body weight in both groups was associated with more volumetric wear. The radiological evidence of prosthetic loosening did not correlate well with clinical evaluation of the hip.

[Demographic profile of the Xavánte Indian population in Sangradouro-Volta Grande, Mato Grosso]. / Perfil demográfico da população indígena Xavánte de Sangradouro-Volta Grande, Mato Grosso (1993-1997), Brasil.

This paper analyzes the demographic profile of the Xavánte population at the Sangradouro-Volta Grande Indigenous Reserve in Mato Grosso, Brazil, from 1993 to 1997. The survey included annual censuses and vital statistics from 7 Xavánte villages. Permanent contact with Brazilian national society, established in the 1940s and 50s, caused a population drop due to epidemics and clashes. In 1995 there were 825 individuals in the community. The crude birth rate (57.7/1,000) and death rate (9.1/1,000) were higher than the national averages. The majority (56%) of the population is under 15 years of age (median: 13 years) and the infant mortality rate is high (87.1 per thousand live births), probably resulting from precarious sanitary conditions in the villages. Other results included the persistence of polygyny; low levels of migration; a dynamic of splits and formation of new villages; traditional housing patterns maintained in the old villages and abandoned in the new ones. The recent demographic recovery in the data from Sangradouro-Volta Grande is similar to that observed in the Pimentel Barbosa community. The study highlights the importance of systematically collecting and analyzing demographic data from indigenous populations.

The bicycle spoke injury: an avoidable accident?

A prospective study of the bicycle spoke injury over a 1-year period included a total of 71 spoke injuries. Of these, 67 injuries occurred on an adult bicycle and four occurred on a child's bicycle. All children sustained ankle and foot injuries, which consisted of contusion and superficial abrasion (N = 45), skin loss (N = 10), skin laceration (N = 4), and undisplaced fractures (N = 12). A biomechanical study was conducted to investigate the use of a protective cover over the wheel to prevent the foot from slipping between the spokes. Wind resistance studies showed that a cover with a mesh size of 10 mm hexagonal could prevent this and at the same time stop the cover from acting as a sail if a flat cover without holes was used instead. The mesh cover, however, will prevent the toes from entering between the spokes but will not prevent the foot from becoming jammed between the wheel and the fork. To prevent this, a plastic shield to bridge the gap between the fork and the horizontal upright has been designed. With these modifications, the bicycle spoke injury can become an injury of the past.

Irreducible dislocation of the fifth metatarsophalangeal joint: a case report.

Metatarsophalangeal joint dislocations are unusual in children and usually occur in the hallux. We are reporting a case of irreducible traumatic dislocation of the fifth metatarsophalangeal joint. At open reduction, the metatarsal head was incarcerated under the flexor digitorum longus. After returning the flexor digitorum longus tendon to its anatomical position, the metatarsophalangeal joint reduced and was stable.