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Mem Inst Oswaldo Cruz ; 105(3): 318-21, 2010 May.
Article in English | MEDLINE | ID: mdl-20512247

ABSTRACT

Thirty-eight strains of Shiga toxin-producing Escherichia coli (STEC) were characterised in terms of biochemical properties, enterohaemolysin production and plasmid carriage. A wide variation in the biochemical properties was observed among the STEC, with 14 distinct biotypes identified. Biotype 1 was the most common, found in 29% of the strains. Enterohaemolysin production was detected in 29% of the strains. Most of the bacterial strains (95%) carried one or more plasmids and considerable heterogeneity in size and combinations was observed. Seven distinct plasmid profiles were identified. The most common profile, characterised by the presence of a single plasmid of ~90 kb, was found in 50% of these strains. These data indicate extensive diversity among STEC strains. No correlation was found among biotype, serotype, enterohaemolysin production and plasmid profile.


Subject(s)
DNA, Bacterial/genetics , Escherichia coli Proteins/biosynthesis , Hemolysin Proteins/biosynthesis , Plasmids/genetics , Shiga-Toxigenic Escherichia coli/genetics , Shiga-Toxigenic Escherichia coli/metabolism , Animals , Cattle , Child , Electrophoresis, Gel, Pulsed-Field , Humans
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