ABSTRACT
The study was undertaken to know the current status of prevalence of gastrointestinal parasites of cattle and buffaloes in Bangalore, Karnataka. An overall prevalence of gastrointestinal parasites among cattle (75.2 %) and buffalos (76.8 %) was determined by coprological examination. The gastrointestinal parasites detected in cattle and buffalo were Strongyle (39.8 and 29.1 %), followed by Amphistome (24.4 and 23.1 %), Moniezia spp. (5.3 and 5.9 %), Fasciola spp. (4.1 and 15.6 %), Trichuris spp. (1.4 and 2.9 %), Buxtonella spp. (36.6 and 37.3 %) and Eimeria spp. (26.7 and 29.8 %) respectively. The percentage prevalence of mixed helminth and protozoan infections was 20.2 and 26.1 % in cattle and buffaloes, respectively.
ABSTRACT
The enzyme-linked immunosorbent assay (ELISA) was evaluated for the diagnosis of Fasciola gigantica infection in cattle and buffaloes. The excretory-secretory (E-S Ag) antigen of F. gigantica adult flukes obtained after invitro incubation was used as an antigen. The test was conducted with 276 sera collected from cattle and buffaloes which included 22 sera each from naturally infected cattle and buffaloes (known positive serum) and with similar number of samples with healthy cattle and buffaloes (known negative serum). The positive results were observed in 18 and 19 of the sera from naturally infected cattle and buffaloes with sensitivity of 81.8 and 86.3 % respectively. Out of 188 serum samples which were found negative on faecal examination 32 (34 %) sera of cattle and 40 (42.5 %) sera of buffaloes were found positive by ELISA respectively. The sensitivity of the test was found to be 91.6 and 95.6 % in cattle and buffaloes respectively.
