Analysis of heavy metals in the endometrial tissue using electron microscope.

OBJECTIVE: This study was done to estimate whether heavy metals in the air may affect endometrial chemical composition. MATERIALS AND METHODS: A total of 30 albino Wistar rats were employed and randomly divided into three groups. The rats of Group 1 and 2 were acutely and sub-chronically exposed to the gas form of heavy metals, respectively. Group 3 was used as a control group. Endometrial tissue worth of heavy metals of the groups was measured in through using scanning electron microscope. RESULTS: A statistically no significant difference was found for endometrial chemical composition of all of the heavy metals (chrome, manganese, iron, cobalt, nickel, zinc and lead) between group 1 and 2 (p>0.05). On the other hand, there was statistically significant difference for all of the heavy metals between group 1 and group 3 (p<0.05), while there was statistically significant difference for chrome, manganese, iron, cobalt and nickel (p<0.05), but zinc and lead (p>0.05) between group 2 and 3. CONCLUSION: Air pollutants of Pb and Zn resulting mostly from combustion of fossil fuels and certain special industrial process in Kirikkale may be a risk factor for the high pregnancy loses by changing endometrial homeostasis.

Examination of the rescue effects of folic acid on derangement of the tubo-ovarian ultrastructural architecture caused by methotrexate.

The purpose of this examination was to observe the effects of folic acid (FA) on methotrexate (MTX)-induced derangements in the fallopian tubes. Investigators in this study sought to explore whether MTX-induced dysfunction in the fallopian tubes would be lessened by the addition of FA to MTX treatment. For this study, 18 albino Wistar rats were randomly divided into 6 groups, each of which comprised 3 rats; 0.1 mg/kg FA, 1 mg/kg MTX + 0.1 mg/kg FA, 5 mg/kg MTX + 0.1 mg/kg FA, 1 mg/kg MTX, and 5 mg/kg MTX were given to groups 2, 3, 4, 5,and 6, respectively; group 1 was the control group. After MTX injection, fallop-ian tube samples from all groups were prepared for examination under electron microscopy. The findings observed in groups 1 and 2 were similar. The level of cellular destruction was greater with the higher doses of MTX without FA; in particular, loss of cilia in the epithelium was prominent in groups 5 and 6. However, there was less cellular destruction observed in groups 3 and 4 than in groups 5 and 6. As a result, the addition of FA should not be overlooked, even when a single-dose MTX regimen is chosen for the treatment of patients with unruptured ectopic pregnancy.

Assessment of DNA damage in postmenopausal women under osteoporosis therapy.

OBJECTIVE: The following study was designed to examine possible DNA damage levels in peripheral blood leukocytes, using the alkaline Comet assay, isolated from postmenopausal women undergoing osteoporosis treatment. STUDY DESIGN: Thirty-two postmenopausal women were randomized into two groups of 16. A dosage of 2.5 mg/day of tibolone (Livial) and 10mg/day of alendronate sodium (Fosamax) were administered to Group 1 over a 12-month period while Group 2 took 10 mg/day of alendronate alone over the same period. The control group consisted of 16 postmenopausal women who did not receive any treatment. Genotoxicity was assessed by the standard method of alkaline Comet assay. RESULTS: When the results of the study groups were compared with those of the control group, significant differences in terms of DNA damage levels were found (p<0.05). However, no difference was detected between Groups 1 and 2 (p>0.05). CONCLUSION: Although, no statistical difference in terms of DNA damage levels between tibolone plus alendronate as opposed to alendronate alone was found, an increase in DNA damage levels was observed in Groups 1 and 2 compared with the control group. Consequently, it can be asserted that the frequency of DNA damage in postmenopausal women with osteoporosis increases under alendronate treatment with or without tibolone.